To study the differences in homing potential between bone marrow cells and umbilical blood cells, CD34 positive cells were obtained from bone marrow (BM) and umbilical blood (UB) by the direct cell separation with dom...To study the differences in homing potential between bone marrow cells and umbilical blood cells, CD34 positive cells were obtained from bone marrow (BM) and umbilical blood (UB) by the direct cell separation with domestic immunomagnetic beads. The expression of the two adhesion molecules CD11a/CD18 and CD44 were examined. After separation, CD34 positive cells accounted for 51 %-82 % of the harvested cells and dye-resistance rate was 82 %-88 %. The expression of CD11a/CD18 and CD44 on the surfaces of UB cells was 49. 6 % 1 10. 2 % and 37. 7 % ± 10. 3 % respectively. On BM cells they were 50. 2 % ± 6. 2 % and 34 % ± 13. 3 % respectively. There were no significant differences in the expression of these two molecules. It was concluded .that the cell separation method with domestic immunomagnetic beads was effective and the stem cells from UB could serve as an alternative source for transplantation.展开更多
An insoluble β cyclodextrin polymer cross linked with epichlorohydrin was prepared, and its structure was identified with infrared spectrum. Colloid stationary phase was prepared by dissolving the polymer in the mixe...An insoluble β cyclodextrin polymer cross linked with epichlorohydrin was prepared, and its structure was identified with infrared spectrum. Colloid stationary phase was prepared by dissolving the polymer in the mixed solvent of diisopropyl ether, methylene dichloride and benzene and treated for 0.5 h by ultrasonication, and then was coated on a fused silica capillary column. The optimun reaction conditions are as follows: the mole ratio of epichlohydrin to β cyclodextrin is 12.1∶1, reacting at 65 ℃ for 24 h. The Chromatographic performance such as column efficiency, thermal stabilities and polarity were studied, two kinds of disubstituted benzene isomers and eight pairs of enantiomers were separated on the capillary column. The results show that the β cyclodextrin polymer is suitable for use as a capillary gas chromatographic stationary phase, and possess excellent chromatographic properties in separating enantiomers and position isomers.展开更多
The problem of separating gravitation from inertia is discussed in very general sense, and the conclusion is positive: man can separate gravitation from inertia, if various observation techniques are applied for. The ...The problem of separating gravitation from inertia is discussed in very general sense, and the conclusion is positive: man can separate gravitation from inertia, if various observation techniques are applied for. The accelerometer's position problem in satellite gravimetry is investigated, and the additional acceleration effect due to the position error of an instrument as well as the difference between the mass center and the gravity center is explored.展开更多
A sufficient and necessary criterion for separability of bipartite quantum states is presented. We construct a class of mixed states in 2 k quantum systems and in 3 k quantum systems, respectively and show that thes...A sufficient and necessary criterion for separability of bipartite quantum states is presented. We construct a class of mixed states in 2 k quantum systems and in 3 k quantum systems, respectively and show that these states are separable if and only if they are positive partial transpositions.展开更多
文摘To study the differences in homing potential between bone marrow cells and umbilical blood cells, CD34 positive cells were obtained from bone marrow (BM) and umbilical blood (UB) by the direct cell separation with domestic immunomagnetic beads. The expression of the two adhesion molecules CD11a/CD18 and CD44 were examined. After separation, CD34 positive cells accounted for 51 %-82 % of the harvested cells and dye-resistance rate was 82 %-88 %. The expression of CD11a/CD18 and CD44 on the surfaces of UB cells was 49. 6 % 1 10. 2 % and 37. 7 % ± 10. 3 % respectively. On BM cells they were 50. 2 % ± 6. 2 % and 34 % ± 13. 3 % respectively. There were no significant differences in the expression of these two molecules. It was concluded .that the cell separation method with domestic immunomagnetic beads was effective and the stem cells from UB could serve as an alternative source for transplantation.
文摘An insoluble β cyclodextrin polymer cross linked with epichlorohydrin was prepared, and its structure was identified with infrared spectrum. Colloid stationary phase was prepared by dissolving the polymer in the mixed solvent of diisopropyl ether, methylene dichloride and benzene and treated for 0.5 h by ultrasonication, and then was coated on a fused silica capillary column. The optimun reaction conditions are as follows: the mole ratio of epichlohydrin to β cyclodextrin is 12.1∶1, reacting at 65 ℃ for 24 h. The Chromatographic performance such as column efficiency, thermal stabilities and polarity were studied, two kinds of disubstituted benzene isomers and eight pairs of enantiomers were separated on the capillary column. The results show that the β cyclodextrin polymer is suitable for use as a capillary gas chromatographic stationary phase, and possess excellent chromatographic properties in separating enantiomers and position isomers.
基金Funded by the National Natural Science Foundation of China (No.40374004)the Foundation of the Key Laboratory of Geo-space Environment and Geodesy, Ministry of Education, China (No.03-04-13).
文摘The problem of separating gravitation from inertia is discussed in very general sense, and the conclusion is positive: man can separate gravitation from inertia, if various observation techniques are applied for. The accelerometer's position problem in satellite gravimetry is investigated, and the additional acceleration effect due to the position error of an instrument as well as the difference between the mass center and the gravity center is explored.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11101017 and 11275131)Beijing Natural Science Foundation Program,and the Scientific Research Key Program of Beijing Municipal Commission of Education(Grant No.KZ201210028032)the Importation and Developmentof High-Caliber Talents Project of Beijing Municipal Institutions(Grant No.CIT&TCD201404067)
文摘A sufficient and necessary criterion for separability of bipartite quantum states is presented. We construct a class of mixed states in 2 k quantum systems and in 3 k quantum systems, respectively and show that these states are separable if and only if they are positive partial transpositions.
