Pancreatic insulin-secretingβcells are essential in maintaining normal glucose homeostasis accomplished byhighly specialized transcription of insulin gene,of which occupies up to 40%their transcriptome.Deficiency of ...Pancreatic insulin-secretingβcells are essential in maintaining normal glucose homeostasis accomplished byhighly specialized transcription of insulin gene,of which occupies up to 40%their transcriptome.Deficiency of these cells causes diabetes mellitus,a global public health problem.Although tremendous endeavors have been made to generate insulin-secreting cells from human pluripotent stem cells(i.e.,primitive cells capable of giving rise to all cell types in the body),a regenerative therapy to diabetes has not yet been established.Furthermore,the nomenclature ofβcells has become inconsistent,confusing and controversial due to the lack of standardized positive controls of developmental stagematched in vivo cells.In order to minimize this negative impact and facilitate critical research in this field,a postgenomic concept of pancreaticβcells might be helpful.In this review article,we will briefly describe howβcells were discovered and islet lineage is developed that may help understand the cause of nomenclatural controversy,suggest a post-genomic definition and finally provide a conclusive remark on future research of this pivotal cell.展开更多
While the importance of molecular marker technology was realized more than two decades ago,high-throughput marker development came into vogue only after the availability of hundreds of thousands of sequences in public...While the importance of molecular marker technology was realized more than two decades ago,high-throughput marker development came into vogue only after the availability of hundreds of thousands of sequences in public databases.Many examples now exist where markers are being used routinely in breeding programs for marker-assisted selection(MAS) of traits of interest or marker assisted recovery of genome of interest.Genetic analysis with thousands to tens of thousands of markers is now possible due to the...展开更多
基金The Juvenile Diabetes Research Foundation(4-2006-1025)Medical Research Foundation of Royal Perth HospitalPerth Children’s Hospital Research Fund(TPCHRF2013)Grant(to F.X.Jiang)partially
文摘Pancreatic insulin-secretingβcells are essential in maintaining normal glucose homeostasis accomplished byhighly specialized transcription of insulin gene,of which occupies up to 40%their transcriptome.Deficiency of these cells causes diabetes mellitus,a global public health problem.Although tremendous endeavors have been made to generate insulin-secreting cells from human pluripotent stem cells(i.e.,primitive cells capable of giving rise to all cell types in the body),a regenerative therapy to diabetes has not yet been established.Furthermore,the nomenclature ofβcells has become inconsistent,confusing and controversial due to the lack of standardized positive controls of developmental stagematched in vivo cells.In order to minimize this negative impact and facilitate critical research in this field,a postgenomic concept of pancreaticβcells might be helpful.In this review article,we will briefly describe howβcells were discovered and islet lineage is developed that may help understand the cause of nomenclatural controversy,suggest a post-genomic definition and finally provide a conclusive remark on future research of this pivotal cell.
文摘While the importance of molecular marker technology was realized more than two decades ago,high-throughput marker development came into vogue only after the availability of hundreds of thousands of sequences in public databases.Many examples now exist where markers are being used routinely in breeding programs for marker-assisted selection(MAS) of traits of interest or marker assisted recovery of genome of interest.Genetic analysis with thousands to tens of thousands of markers is now possible due to the...
