OBJECTIVE To study the protective effect of potassium 2-(l-hydroxypentyl)-benzoate(PHPB) on hippocampal neurons,synapses and dystrophic axons in APP/PS1 mice.METHODS Ten-month-old male APP/PS1 transgenic mice and age-...OBJECTIVE To study the protective effect of potassium 2-(l-hydroxypentyl)-benzoate(PHPB) on hippocampal neurons,synapses and dystrophic axons in APP/PS1 mice.METHODS Ten-month-old male APP/PS1 transgenic mice and age-matched wild-type mice were randomly divided into three groups:wild-type group(WT Con group,n=10),APP/PS1 group(Tg Con group,n=10) and PHPB treated APP/PS1 group(PHPB group,n=10).PHPB group received 30 mg · kg-1 PHPB by oral gavage once daily for 3 months.WT Con group and Tg Con group received the same volume of water.Three months later,mice were sacrificed for biochemical and pathological testing such as transmission electron microscopy,Golgi staining and Western boltting analysis.RESULTS Under the transmission electron microscope,most hippocampal neurons and subcel ular organel es in WT Con group exhibited normal morphology.However,the degenerative changes were observed in Tg Con group such as nuclear fragmentation,mitochondrial swelling,ribosomes detachment and autophagic vacuoles accumulation.The hippocampal synapses number and the thickness of postsynaptic density(PSD) were significantly decreased in Tg Con group compared with the WT Con group(P<0.05).After PHPB treatment,the degenerative changes in APP/PS1 mice were alleviated to some extent.The synapse number has been elevated significantly(P<0.05) and the PSD has been thickened as well.Golgi staining showed that the spine density of secondary and tertiary apical dendritic branches was significantly decreased in CA1 and DG areas of Tg Con group(P<0.05).Sholl analysis revealed a decrease of dendritic complexity in Tg Con group compared with WT Con group(P<0.05).These abnormalities were alleviated to some extent after PHPB treatment.Western blotting study showed that the protein levels of synaptic marker PSD-95 and synaptophysin were significantly decreased in the hippocampus of Tg Con group(P<0.05).A significant increase of PSD-95(P<0.05) and a slight increase of SYP were observed after the PHPB treatment.Besides,we found a significant increase in the ratio of LC3-Ⅱ/LC3-Ⅰ in Tg Con group compared with the WT Con group(P<0.01) and the relevant improvement after PHPB treatment(P<0.05),which showed the regulatory effect of PHPB on autophagy impairment.CONCLUSION PHPB showed protective effects on hippocampal neurons,synapses and dystrophic axons in APP/PS1 mice,which might help explain its role on cognitive improvement in Alzheimer disease treatment.展开更多
特定模数硅酸钾溶液（PS）已广泛的用于我国西北干旱地区土遗址加固保护工程中,并且取得了显著的效果。但是PS对潮湿地区遗址土加固效果,目前尚缺乏成熟的理论和实验验证。本文从室内试验的资料出发,研究PS对潮湿地区土遗址非饱和土的...特定模数硅酸钾溶液（PS）已广泛的用于我国西北干旱地区土遗址加固保护工程中,并且取得了显著的效果。但是PS对潮湿地区遗址土加固效果,目前尚缺乏成熟的理论和实验验证。本文从室内试验的资料出发,研究PS对潮湿地区土遗址非饱和土的增强效应。本次研究土样取自位于潮湿环境中的浙江杭州良渚土遗址（3000 B C.）,在实验室内对高含水量（13%~25%）的重塑土样进行了PS渗透加固。通过研究对比加固前后重塑土样的固结特性和抗剪强度,发现PS对潮湿地区遗址土也有一定的加固效果。加固效果随初始饱和度的不同而不同,但初始含水量达25%的土在PS作用下仍可能得到增强。经PS渗透的土样不仅提高了初始固结性能和抗剪强度,而且加固土样而且呈现出一定的弹脆性特征,呈现出塑脆性转化,表明PS渗透可能产生对土初始结构的稳固作用。这一探索研究将为扩大PS在土遗址加固保护工程的应用提供一定的理论支持。展开更多
基于室内物理模型试验和原位锚固试验,对基于高模数硅酸钾溶液-（粉煤灰＋粉土）（PS-（F＋C））浆液的木锚杆锚固系统在夯土介质中进行了拉拔测试与杆体-浆体界面应变监测,研究了该锚固系统的锚固性能与破坏模式、杆体-浆体界面剪应力...基于室内物理模型试验和原位锚固试验,对基于高模数硅酸钾溶液-（粉煤灰＋粉土）（PS-（F＋C））浆液的木锚杆锚固系统在夯土介质中进行了拉拔测试与杆体-浆体界面应变监测,研究了该锚固系统的锚固性能与破坏模式、杆体-浆体界面剪应力分布与传递特征。结果表明：该锚固系统室内试验极限锚固力（24～38 k N）远大于现场试验值（2.5～8 k N）;锚固系统具有低弹性强塑性特征,表现出极强的延性;在荷载进程中杆体-浆体界面的应力分布与传递特征具有多峰值分布、高值往往出现在锚固末端、压应力出现等特征,表明该锚固系统兼有拉力型和压力型全长黏结性锚固系统的特点;该系统适合于夯筑土遗址锚固,与遗址具有良好的物理力学兼容性。展开更多
The phase behavior and property of aqueous two-phase system formed by SS(PS)/CTAB/NaBr/H2O mixture had been studied.The results indicated that there was only one ATPS region in the both systems,and the system with PS ...The phase behavior and property of aqueous two-phase system formed by SS(PS)/CTAB/NaBr/H2O mixture had been studied.The results indicated that there was only one ATPS region in the both systems,and the system with PS showed a peculiar inversion of ATPS.As a function of composition,the volume ratio appeared a turn on its relation curve.It was surposed that one side of the turn was ATPS which contains cubic liquid crystal,the other dosen’t.When the ratio of top vulume to bottom volume was close to 1,the ATPS forming time was the shortest,and vice versa.展开更多
基金National Natural Sciences Foundation of China(8147320081673420)CAMS InnovationFund for Medical Sciences (2017-I2M-2-004).
文摘OBJECTIVE To study the protective effect of potassium 2-(l-hydroxypentyl)-benzoate(PHPB) on hippocampal neurons,synapses and dystrophic axons in APP/PS1 mice.METHODS Ten-month-old male APP/PS1 transgenic mice and age-matched wild-type mice were randomly divided into three groups:wild-type group(WT Con group,n=10),APP/PS1 group(Tg Con group,n=10) and PHPB treated APP/PS1 group(PHPB group,n=10).PHPB group received 30 mg · kg-1 PHPB by oral gavage once daily for 3 months.WT Con group and Tg Con group received the same volume of water.Three months later,mice were sacrificed for biochemical and pathological testing such as transmission electron microscopy,Golgi staining and Western boltting analysis.RESULTS Under the transmission electron microscope,most hippocampal neurons and subcel ular organel es in WT Con group exhibited normal morphology.However,the degenerative changes were observed in Tg Con group such as nuclear fragmentation,mitochondrial swelling,ribosomes detachment and autophagic vacuoles accumulation.The hippocampal synapses number and the thickness of postsynaptic density(PSD) were significantly decreased in Tg Con group compared with the WT Con group(P<0.05).After PHPB treatment,the degenerative changes in APP/PS1 mice were alleviated to some extent.The synapse number has been elevated significantly(P<0.05) and the PSD has been thickened as well.Golgi staining showed that the spine density of secondary and tertiary apical dendritic branches was significantly decreased in CA1 and DG areas of Tg Con group(P<0.05).Sholl analysis revealed a decrease of dendritic complexity in Tg Con group compared with WT Con group(P<0.05).These abnormalities were alleviated to some extent after PHPB treatment.Western blotting study showed that the protein levels of synaptic marker PSD-95 and synaptophysin were significantly decreased in the hippocampus of Tg Con group(P<0.05).A significant increase of PSD-95(P<0.05) and a slight increase of SYP were observed after the PHPB treatment.Besides,we found a significant increase in the ratio of LC3-Ⅱ/LC3-Ⅰ in Tg Con group compared with the WT Con group(P<0.01) and the relevant improvement after PHPB treatment(P<0.05),which showed the regulatory effect of PHPB on autophagy impairment.CONCLUSION PHPB showed protective effects on hippocampal neurons,synapses and dystrophic axons in APP/PS1 mice,which might help explain its role on cognitive improvement in Alzheimer disease treatment.
文摘特定模数硅酸钾溶液（PS）已广泛的用于我国西北干旱地区土遗址加固保护工程中,并且取得了显著的效果。但是PS对潮湿地区遗址土加固效果,目前尚缺乏成熟的理论和实验验证。本文从室内试验的资料出发,研究PS对潮湿地区土遗址非饱和土的增强效应。本次研究土样取自位于潮湿环境中的浙江杭州良渚土遗址（3000 B C.）,在实验室内对高含水量（13%~25%）的重塑土样进行了PS渗透加固。通过研究对比加固前后重塑土样的固结特性和抗剪强度,发现PS对潮湿地区遗址土也有一定的加固效果。加固效果随初始饱和度的不同而不同,但初始含水量达25%的土在PS作用下仍可能得到增强。经PS渗透的土样不仅提高了初始固结性能和抗剪强度,而且加固土样而且呈现出一定的弹脆性特征,呈现出塑脆性转化,表明PS渗透可能产生对土初始结构的稳固作用。这一探索研究将为扩大PS在土遗址加固保护工程的应用提供一定的理论支持。
文摘基于室内物理模型试验和原位锚固试验,对基于高模数硅酸钾溶液-（粉煤灰＋粉土）（PS-（F＋C））浆液的木锚杆锚固系统在夯土介质中进行了拉拔测试与杆体-浆体界面应变监测,研究了该锚固系统的锚固性能与破坏模式、杆体-浆体界面剪应力分布与传递特征。结果表明：该锚固系统室内试验极限锚固力（24～38 k N）远大于现场试验值（2.5～8 k N）;锚固系统具有低弹性强塑性特征,表现出极强的延性;在荷载进程中杆体-浆体界面的应力分布与传递特征具有多峰值分布、高值往往出现在锚固末端、压应力出现等特征,表明该锚固系统兼有拉力型和压力型全长黏结性锚固系统的特点;该系统适合于夯筑土遗址锚固,与遗址具有良好的物理力学兼容性。
文摘The phase behavior and property of aqueous two-phase system formed by SS(PS)/CTAB/NaBr/H2O mixture had been studied.The results indicated that there was only one ATPS region in the both systems,and the system with PS showed a peculiar inversion of ATPS.As a function of composition,the volume ratio appeared a turn on its relation curve.It was surposed that one side of the turn was ATPS which contains cubic liquid crystal,the other dosen’t.When the ratio of top vulume to bottom volume was close to 1,the ATPS forming time was the shortest,and vice versa.
