Medicinal plants are popular and widely used as a major source of herbal drugs and pharmaceutical compounds. Ever-growing demands make medicinal plants faced to several problems including efficacy and safety to meet b...Medicinal plants are popular and widely used as a major source of herbal drugs and pharmaceutical compounds. Ever-growing demands make medicinal plants faced to several problems including efficacy and safety to meet business requirements, conservation, and artificially assisted breeding. As a powerful molecular tool, microsatellites offer the great potentials for various purposes in plants. This review provides a scenario of microsatellites in medicinal plants including development from genomic or expressed sequence tag libraries, cross-species transferability, genotyping, and potential applications. We emphasized on the authentication of medicinal plants by microsatellite markers.展开更多
Objective：To test whether Shenfu Injection（参附注射液,SFI）might attenuate the impact of cerebral energy dysfunction after resuscitation in a pig model of cardiac arrest（CA）.Methods：Thirty-four Wuzhishan miniatur...Objective：To test whether Shenfu Injection（参附注射液,SFI）might attenuate the impact of cerebral energy dysfunction after resuscitation in a pig model of cardiac arrest（CA）.Methods：Thirty-four Wuzhishan miniature inbred pigs were randomly divided into three groups：the SFI group（n=12）,the saline group（SA group,n=12）,and the sham-operated group（sham group,n=10）.Following successful return of spontaneous circulation（ROSC）from 8-min untreated ventricular fibrillation,animals received a continuous infusion of either SFI（0.2 mL/min）or saline for 6 h.Cerebral performance category score was evaluated at 24and 48 h after ROSC,followed by positron emission tomography and computed tomography scans of cerebral glucose uptake.Surviving pigs were euthanized 48 h after ROSC,and the brains were removed for detecting mitochondrial function.Results：Compared with the SA group,SFI treatment produced a better neurologic outcome48 h after ROSC（P〈0.05）.However,there was no significant difference of survival rate between the SA and SFI groups（83.3%vs.81.8%,P〉0.05）.After ROSC,the SA group showed a decrease in the maximum standardized uptake value of different regions in the brain tissue,where SFI treatment can ameliorate these decreases（P〈0.01or P〈0.05）.Improved mitochondrial respiratory properties and higher mitochondrial membrane potential were also found following SFI treatment compared with the SA group at 48 h after ROSC（P〈0.05 or P〈0.01）.Conclusion：SFI treatment after resuscitation has significant neuroprotective effects against disruption of cerebral energy metabolism from CA by improving glucose uptake and by normalizing mitochondrial function.展开更多
基金National Natural Science Foundation of China(31270340,31200225,30800624)Instrument Developing Project of the Chinese Academy of Sciences(YZ201227)+1 种基金the Key Research Program of the Chinese Academy of Sciences(Grant NO.KSZD-EW-Z-004)Grant from Zhongning County
文摘Medicinal plants are popular and widely used as a major source of herbal drugs and pharmaceutical compounds. Ever-growing demands make medicinal plants faced to several problems including efficacy and safety to meet business requirements, conservation, and artificially assisted breeding. As a powerful molecular tool, microsatellites offer the great potentials for various purposes in plants. This review provides a scenario of microsatellites in medicinal plants including development from genomic or expressed sequence tag libraries, cross-species transferability, genotyping, and potential applications. We emphasized on the authentication of medicinal plants by microsatellite markers.
基金Supported by the Beijing Natural Science Foundation(No.7132092)Beijing Scientific Research Project for Outstanding Doctoral Thesis Guidance Teacher(No.20121002501)
文摘Objective：To test whether Shenfu Injection（参附注射液,SFI）might attenuate the impact of cerebral energy dysfunction after resuscitation in a pig model of cardiac arrest（CA）.Methods：Thirty-four Wuzhishan miniature inbred pigs were randomly divided into three groups：the SFI group（n=12）,the saline group（SA group,n=12）,and the sham-operated group（sham group,n=10）.Following successful return of spontaneous circulation（ROSC）from 8-min untreated ventricular fibrillation,animals received a continuous infusion of either SFI（0.2 mL/min）or saline for 6 h.Cerebral performance category score was evaluated at 24and 48 h after ROSC,followed by positron emission tomography and computed tomography scans of cerebral glucose uptake.Surviving pigs were euthanized 48 h after ROSC,and the brains were removed for detecting mitochondrial function.Results：Compared with the SA group,SFI treatment produced a better neurologic outcome48 h after ROSC（P〈0.05）.However,there was no significant difference of survival rate between the SA and SFI groups（83.3%vs.81.8%,P〉0.05）.After ROSC,the SA group showed a decrease in the maximum standardized uptake value of different regions in the brain tissue,where SFI treatment can ameliorate these decreases（P〈0.01or P〈0.05）.Improved mitochondrial respiratory properties and higher mitochondrial membrane potential were also found following SFI treatment compared with the SA group at 48 h after ROSC（P〈0.05 or P〈0.01）.Conclusion：SFI treatment after resuscitation has significant neuroprotective effects against disruption of cerebral energy metabolism from CA by improving glucose uptake and by normalizing mitochondrial function.
