On Potentially Graphical Sequences of G-E(H)

A loopless graph on n vertices in which vertices are connected at least by a and at most by b edges is called a(a,b,n)-graph. A(b,b,n)-graph is called(b,n)-graph and is denoted by K_n^b(it is a complete graph), its complement by K_n^b. A non increasing sequence π =(d_1,…,d_n) of nonnegative integers is said to be(a,b,n) graphic if it is realizable by an(a,b,n)-graph. We say a simple graphic sequence π=(d_1,…,d_n) is potentially K_4-K_2∪K_2-graphic if it has a a realization containing an K_4-K_2∪K_2 as a subgraph where K_4 is a complete graph on four vertices and K_2∪K_2 is a set of independent edges. In this paper, we find the smallest degree sum such that every n-term graphical sequence contains K_4-K_2∪K_2 as subgraph.

Characterizing C6＋P2-graphic Sequences

For a given graph H, a graphic sequence π =(d1, d2, ···, dn) is said to be potentially H-graphic if π has a realization containing H as a subgraph. In this paper, we characterize the potentially C6+ P2-graphic sequences where C6+ P2 denotes the graph obtained from C6 by adding two adjacent edges to the three pairwise nonadjacent vertices of C6. Moreover, we use the characterization to determine the value of σ(C6+ P2, n).

The transcriptome analysis of cleft lip/palate-related PTCH1 variants in GMSM-K cells show carcinogenic potential

Cancer progression involves the sonic hedgehog(SHH)pathway,in which the receptor PTCH1 actives the downstream pathways.Dysfunction of PTCH1 can lead to nevoid basal cell carcinoma Syndrome(NBCCs)including neoplastic disease and congenital disorder.To evaluate the relationship between PTCH1 and cancer,we applied the CRISPR/Cas9 system to knock out PTCH1 in oral nontumorous epithelial cells(GMSM-K).Then we screened six PTCH1 variants associated with cleft lip/palate(CL/P),one of the congenital disorders in NBCCs,and generated PTCH1 variant and wild-type recombinant PTCH1^(−/−)GMSM-K cell lines.Transcriptome sequencing was conducted in these cell lines.The results revealed that differentially expressed genes(DEGs)in PTCH1^(−/−)GMSM-K were enriched in extracellular compartments,contributing epithelial diseases by pathway enrichment analysis.RT-PCR confirmed that KRT34,KRT81,KRT86,PDGFB,and WNT10B genes,associated with extracellular compartments were highly expressed in PTCH1^(−/−).The Kyoto Encyclopedia of Genes and Genomes analysis also suggested that DEGs are closely related to focal adhesion,transcriptional misregulation,and proteoglycans in breast and gastric cancers.Comparative analysis of samples revealed that the CL/P-associated PTCH1 variants A443G and V908G are potentially carcinogenic.These findings provide new insights into the carcinogenic potential of PTCH1 dysfunction.

一种适用三电平并联系统的虚拟矢量调制策略

直流侧中点电位平衡和零序环流抑制是保证三电平逆变器并联系统正常运行的重要问题,然而基于冗余小矢量对调节的环流抑制方案进一步增加空间矢量脉宽调制(space vector pulse width modulation,SVPWM)中点电流无法对称消除的场景,加剧双机系统中点电位不平衡程度。该文基于虚拟矢量合成思路,提出一种满足零序环流抑制和中点电位平衡需求的双机虚拟矢量调制方法。该方法重新定义虚拟中矢量,通过缩短中矢量作用时间和增加参与合成的对称冗余小矢量以减少中点电流不平衡场景;分析不同扇区不同控制目标下虚拟矢量合成规则,利用相占空比法优化虚拟矢量的作用顺序,减少矢量合成过程中的开关次数。与传统SVPWM策略下中点电压变化量的对比分析表明,所提方法能够削弱环流抑制和中点电位平衡的耦合关系。最后,利用Starsim平台实验验证该策略的有效性。

水华对中华绒螯蟹(Eriocheir sinensis)池塘养殖系统细菌群落及潜在致病菌的影响

近年来,水华频发制约着中华绒螯蟹养殖业的发展,环境微生物群落响应水华发生与消退机制是预防与治理水华的重要组成部分。为了解析中华绒螯蟹养殖系统细菌群落对水华的响应特征,采用16S rRNA基因扩增测序技术对拟浮丝藻水华下中华绒螯蟹养殖池塘细菌群落的组成、结构和潜在病原菌的变化进行了研究。结果显示,水体中的优势细菌类群为伯克霍尔德菌科(Burkholderiaceae)和微杆菌科(Microbacteriaaceae),底泥中的优势类群为Steroidobacteraceae菌科和Burkholderiaceae等;科水平下的蓝细菌门类群和其他大部分细菌群落呈显著正相关。养殖水体中潜在病原菌的丰度与水华的生消呈现出相反的趋势,OTU365和OTU1614(均属于Candidatus Similichlamydia epinepheli)是其主要的潜在病原菌,而在底泥中潜在致病菌表现出富集的特征,以OTU1280(大肠杆菌,Escherichia coli)为主。CCA结果显示,蓝藻暴发过程中,Tem、NO3--N、NO2--N及NH4+-N驱使水体蓝藻门细菌类群的变异,NO2--N是驱动养殖水体潜在致病菌群落变异的主导因子。研究结果为防治中华绒螯蟹养殖水华发生提供了宝贵的数据支撑。

基于潜力类型差异化的农村居民点建设用地整治潜力测算及释放——以华南丘陵区某县为例

从其他闲散用地、闲置建设用地及超标准宅基地3个角度对农村居民点建设用地整治潜力进行测算,从经济角度探索区域整治效益,并结合整治潜力、效益确定建设用地整治时序。结果表明,研究区农村居民点建设用地整治潜力充足,其中超标准宅基地整治潜力显著;其他闲散用地及闲置建设用地整治效益优越,超标准宅基地整治收支差异明显;农村居民点建设用地整治潜力释放大小与整治面积、效益、时序相关,由地区能力与需求决定。

转录组测序分析黄连总碱抗脑缺血的作用机制

背景:黄连清热燥湿、泻火解毒,黄连及其成分对脑缺血有显著的保护作用,基于网络药理学和转录组测序探究黄连总碱抗脑缺血的作用机制。目的:在明确黄连总碱对脑缺血大鼠保护作用的基础上,基于网络药理学和转录组测序技术探讨黄连总碱干预脑缺血的作用机制。方法:将SD大鼠随机分为假手术组、缺血再灌注组、阳性药物组和黄连总碱组,后3组采用改良线栓法制备大脑中动脉阻塞的缺血再灌注模型,假手术组不插入线栓,其余手术操作相同。通过TTC染色、Longa 5神经功能缺损评分、苏木精-伊红染色、尼氏染色评价黄连总碱对脑缺血再灌注模型大鼠的脑保护作用。对假手术组、缺血再灌注组、黄连总碱组大鼠脑组织进行转录组测序,通过差异表达基因筛选、基因本体论分析、京都基因与基因组百科全书分析以及转录组学与网络药理学关联分析,阐明黄连总碱干预脑缺血的作用特点,最后通过ELISA检测及免疫荧光染色对黄连总碱干预脑缺血的关键靶点进行验证。结果与结论:①黄连总碱可降低缺血再灌注模型大鼠Longa 5神经功能缺损评分及脑梗死面积,增加神经元、尼氏小体数目;②黄连总碱治疗后差异表达基因的基因本体论功能富集分析包括炎症反应、分裂原活化蛋白激酶级联的正调控等生物学过程;京都基因与基因组百科全书通路富集分析主要涉及白细胞介素17信号通路、神经活性配体-受体相互作用、环磷腺苷信号通路等通路;③转录组分析发现黄连总碱主要调控的基因有前列腺素内过氧化物合酶2、脑源性神经营养因子、瞬间受体电位A1等;④采用网络药理学分析发现,黄连总碱中9个成分可能通过87个成分靶点关联到过氧化物合酶2、脑源性神经营养因子及瞬间受体电位A1而发挥作用;⑤ELISA检测及免疫荧光染色结果进一步证实,黄连总碱调控脑缺血再灌注模型大鼠过氧化物合酶2、脑源性神经营养因子和瞬间受体电位A1的表达;⑥提示黄连总碱能明显改善脑缺血再灌注模型大鼠损伤,可能通过调控过氧化物合酶2、脑源性神经营养因子和瞬间受体电位A1而发挥作用。

The Erds-Jacobson-Lehel conjecture on potentially P_k-graphic sequence is true

A variation in the classical Turn extremal problem is studied. A simple graph G of order n is said to have property P k if it contains a clique of size k+1 as its subgraph. An n term nonincreasing nonnegative integer sequence π=(d 1,d 2,...,d n) is said to be graphic if it is the degree sequence of a simple graph G of order n and such a graph G is referred to as a realization of π . A graphic sequence π is said to be potentially P k graphic if it has a realization G having property P k . The problem: determine the smallest positive even number σ(k,n) such that every n term graphic sequence π=(d 1,d 2,...,d n) without zero terms and with degree sum σ(π)=d 1+d 2+...+d n at least σ(k,n) is potentially P k graphic has been proved positive.

The smallest degree sum that yields potentially K_(r,r)-graphic sequences

We consider a variation of a classical Turán-type extremal problem as follows: Determine the smallest even integer σ(Kr,r,n) such that every n-term graphic sequence π = (d1,d2,...,dn) with term sum σ(π) = d1 + d2 + ... + dn ≥ σ(Kr,r,n) is potentially Kr,r-graphic, where Kr,r is an r × r complete bipartite graph, i.e. π has a realization G containing Kr,r as its subgraph. In this paper, the values σ(Kr,r,n) for even r and n ≥ 4r2 - r - 6 and for odd r and n ≥ 4r2 + 3r - 8 are determined.

The Smallest Degree Sum That Yields Potentially Kr＋1 - K3-Graphic Sequences

Let a（Kr,＋1 - K3,n） be the smallest even integer such that each n-term graphic sequence п= （d1,d2,…dn） with term sum σ（п） = d1 ＋ d2 ＋…＋ dn 〉 σ（Kr＋1 -K3,n） has a realization containing Kr＋1 - K3 as a subgraph, where Kr＋1 -K3 is a graph obtained from a complete graph Kr＋1 by deleting three edges which form a triangle. In this paper, we determine the value σ（Kr＋1 - K3,n） for r ≥ 3 and n ≥ 3r＋ 5.

On-line Monitoring for Phosphorus Removal Process and Bacterial Community in Sequencing Batch Reactor

For efficient energy consumption and control of effluent quality, the cycle duration for a sequencing batch reactor (SBR) needs to be adjusted by real-time control according to the characteristics and loading of waste-water. In this study, an on-line information system for phosphorus removal processes was established. Based on the analysis for four systems with different ecological community structures and two operation modes, anaerobic-aerobic process and anaerobic-anaerobic process, the characteristic patterns of oxidation-reduction potential (ORP) and pH were related to phosphorous dynamics in the anaerobic, anoxic and aerobic phases, for determination of the end of phosphorous removal. In the operation mode of anaerobic-aerobic process, the pH profile in the anaerobic phase was used to estimate the relative amount of phosphorous accumulating organisms (PAOs) and glycogen accumulat-ing organisms (GAOs), which is beneficial to early detection of ecology community shifts. The on-line sensor val-ues of pH and ORP may be used as the parameters to adjust the duration for phosphorous removal and community shifts to cope with influent variations and maintain appropriate operation conditions.

Knowledge-based potentials in bioinformatics: From a physicist's viewpoint

Biological raw data are growing exponentially, providing a large amount of information on what life is. It is believed that potential functions and the rules governing protein behaviors can be revealed from analysis on known native structures of proteins. Many knowledge-based potentials for proteins have been proposed. Contrary to most existing review articles which mainly describe technical details and applications of various potential models, the main foci for the discussion here are ideas and concepts involving the construction of potentials, including the relation between free energy and energy, the additivity of potentials of mean force and some key issues in potential construction. Sequence analysis is briefly viewed from an energetic viewpoint.

On a Problem of Potentially Pancyclic-graphic Sequences Due to S.B. Rao

A non-increasing sequenceπ=(d_1,d_2,···,d_n)of nonnegative integers is said to be potentially hamiltonian-graphic(resp.potentially pancyclic-graphic)if it is realizable by a simple graph on n vertices containing a hamiltonian cycle(resp.containing cycles of every length from 3 to n).A.R.Rao and S.B.Rao(J.Combin.Theory Ser.B,13(1972),185–191)and Kundu(Discrete Math.,6(1973),367–376)presented a characterization ofπ=(d_1,d_2,···,d_n)that is potentially hamiltonian-graphic.S.B.Rao(Lecture Notes in Math.,No.855,Springer Verlag,1981,417–440,Unsolved Problem 2)further posed the following problem:present a characterization ofπ=(d_1,d_2,···,d_n)that is potentially pancyclic-graphic.In this paper,we first give solution to this problem for the case of 4≤n≤11.Moreover,we also show that a near regular graphic sequenceπ=(d_1,d_2,···,d_n)with dn≥3 is potentially pancyclic-graphic.

A Characterization for a Sequence to be Potentially K_(r+1) — e-graphic

Let n 〉 r, let lr --- （dl,d2,-,dn） be a non-increasing sequence of nonnegative integers and let Kr＋l - e be the graph obtained from Kr＋l by deleting one edge. If zr has a realization G containing Kr＋l - e as a subgraph, then r is said to be potentially Kr＋l - e-graphic. In this paper, we give a characterization for a sequence π to be potentially Kr＋l - e-graphic.

A neutral-point potential balance control strategy for three-level inverter based on VSVPWM

The topology of diode neutral-point-clamped(NPC)three-level inverter is prone to neutral-point potential offset.When the sum of three-phase current is zero,the virtual space vector pulse width modulation(VSVPWM)scheme does not cause the neutral-point voltage offset,but it lacks the ability to balance the deviation.For this reason,a neutral-point potential control strategy combining virtual space vector modulation and loop width control is proposed.The neutral-point potential is balanced by introducing the distribution factor for the regions with redundant vectors.For other regions,the potential is controlled by selecting a suitable switching sequence.Meanwhile,the effect on the virtual vector modulation is reduced within the loop width by setting an appropriate loop width,thereby improving the balance effect.The simulation results show that the proposed method has a strong ability to control the offset and has excellent potential balance performance under the conditions of balanced load,unbalanced load and asymmetric capacitance parameters.

靶向二代测序在骨髓增生异常综合征患者中的诊断及预后意义

目的探讨靶向二代测序(next-generation sequencing,NGS)在骨髓增生异常综合征(myelodysplastic syndrome,MDS)患者中的诊断及预后意义。方法回顾性分析淮安市第一人民医院血液科2018年1月—2021年9月收治的205例疑诊MDS的血细胞减少患者的靶向NGS数据资料,观察患者的诊断分布、髓系克隆性疾病患者的基因突变特点;采用ROC曲线分析基因突变数目及等位基因变异频率(variant allele fraction,VAF)预测MDS和MDS/MPN的诊断潜能;并采用Kaplan-Meier生存分析法研究突变数目和VAF在初诊MDS患者中的预后意义。结果205例患者包括40例意义未明的特发性血细胞减少症(idiopathic cytopenia of undetermined significance,ICUS)、47例再生障碍性贫血(aplastic anemia,AA)、23例意义未明的克隆性血细胞减少症(clonal cytopenias of undetermined significance,CCUS)、79例MDS、13例骨髓增生异常-骨髓增殖性肿瘤(myelodysplastic/myeloproliferative neoplasms,MDS/MPN)和3例MPN患者。其中,以髓系克隆造血为表现的MDS/MPN、MDS和CCUS 3组患者的VAF的平均值分别为45.12%(7.90%~93.60%)、44.79%(8.70%~99.70%)和34.83%(4.40%~94.60%)(P=0.1778);3组突变数目的平均值分别为2.462(0~7)、1.886(0~7)和1.826(1~4)(P=0.4593)。诊断方面:VAF预测MDS及MDS/MPN的敏感度为78.9%,特异度为80.0%,最佳截断值=6.6%;基因突变数目的敏感度为78.9%,特异度为78.2%,最佳截断值=1;然而2个指标联合并不能提高MDS及MDS/MPN的诊断效能。MDS预后方面:VAF≥57.93%组(n=13)患者较VAF<57.93%组(n=66)的患者预后更差;中位OS显著缩短(9.0个月vs未达到,P=0.0005);中位PFS也显著缩短(11.0个月vs未达到,P=0.0079)。基因突变数目≥2(n=42)的患者较突变数目<2(n=37)的患者预后差;中位OS显著缩短(33.0个月vs未达到,P=0.0265);而中位PFS差异无统计学意义(P=0.6067)。结论本研究的靶向NGS谱简洁实用,基因突变数目和VAF对MDS患者的诊断预测和预后判断具有重要的临床意义。

西准噶尔地区地球化学元素序结构特征及其地质意义

地球化学元素序结构分析是本团队研制的一套用于地球化学数据信息挖掘与地质分析的新技术方法。本文利用西准噶尔地区1∶20万化探数据,从地球化学元素序结构特征的角度,分析了该区的成矿地球化学背景、主要成矿元素异常分布及异常地质特征等,并对其找矿潜力作出评价。分析认为,西准噶尔地区主要矿床类型应为中低温热液矿床,区内Au、Ag、As-Sb-Hg、Mo-W-Bi和U-Be等元素或元素组合异常表现较为强烈,具有较好的找矿潜力。

以广泛多房囊性结构为特征的肾肿瘤的临床病理观察

目的探讨以广泛多房囊性结构为特征的肾肿瘤的临床病理特征。方法回顾性分析本院以广泛多房囊性结构为特征的肾肿瘤,包括:1例MED15-TFE3融合基因的Xp11易位肾细胞癌(RCC)、1例管状囊性肾细胞癌(TCRCC)及6例低度恶性潜能的多房囊性肾肿瘤(MCRN-LMP)的临床资料、病理检查及分子检测结果。结果入组的3种肾肿瘤大体均为境界清楚的广泛多房囊性结构。其中MED15-TFE3融合基因的RCC镜下可见胞质透明到嗜酸性的低级别肿瘤细胞呈乳头状、腺泡状或者巢状分布于纤维囊壁;砂砾体易见;免疫组化特征性显示TFE3强阳性表达;高通量测序结果显示MED15-TFE3基因融合。TCRCC巨检有特征性的海绵状外观,镜下可见肿瘤由厚薄不一的纤维囊壁分隔成大小不等的管囊结构,囊腔壁可见扁平至靴钉样的嗜酸性肿瘤细胞;免疫组化染色Cathepsin K、CD10、P504s、Pax-8、Vimentin阳性表达,TFE3和TFEB均为阴性表达。MCRN-LMP镜下观察肿瘤也是由纤细的纤维间隔分隔,囊壁被覆单层透明低级别肿瘤细胞,纤维间隔内无膨胀性生长肿瘤细胞结节;免疫组化显示肿瘤细胞PAX8和CA9强阳性表达,TFE3和TFEB均为阴性表达。结论MED15-TFE3融合基因的Xp11易位RCC、TCRCC和MCRN-LMP大体上均是广泛多房囊状结构,组织学形态也部分相似,但是免疫组化及分子结果有所不同,有助于鉴别诊断。

基于CEEMDAN的压裂装备潜在性故障诊断模型

压裂装备的运行受力学负载作用,易出现潜在性故障。为保证压裂装备稳定运行,构建一种基于CEEMDAN的压裂装备潜在性故障诊断模型。按照CEEMDAN分解原则求解排列熵参数,确定压裂装备潜在性故障序列分解条件。提取潜在性故障的鲁棒局部均值,定义诊断模型的瞬态特征,并联合其他关联诊断条件完成压裂装备潜在性故障诊断。实验结果表明,应用构建模型后,故障灯亮起时的力学载荷作用仅为5.7×10^(9)N,诊断结果与发生潜在性故障时的压裂装备额定载荷作用条件相同,符合精确诊断的实际应用需求。

亚成体与老年体圈养大熊猫的口腔菌群多样性及潜在致病菌分析

采用高通量测序技术对6只亚成体和老年体的圈养大熊猫口腔唾液样本进行测序,分析圈养大熊猫口腔细菌的菌群多样性、结构和潜在致病菌及其在2组不同年龄群体间的差异。结果显示:大熊猫口腔中的细菌以变形菌门(Proteobacteria)和蓝细菌门(Cyanobacteria)等为主,相对丰度分别为70.4%、13.4%,在属水平上主要有放线杆菌属(Actinobacillus)、劳特罗普氏菌属(Lautropia)、假单胞菌属(Pseudomonas)和莫拉氏菌属(Moraxella)等,相对丰度分别为10.5%、5.4%、3.9%、3.8%;亚成体大熊猫口腔菌群的多样性和丰富度高于老年体的,但差异无统计学意义;β多样性分析结果显示,2组大熊猫口腔菌群结构间的差异无统计学意义;LEfSe分析发现2组之间的差异细菌在亚成体组中有Niveispirillum、固氮螺菌属(Azospirillum)与根瘤菌属(Rhizobium),在老年体组中则有气单胞菌属(Arenimonas)和二氧化碳噬纤维菌属(Capnocytophaga),但它们的相对丰度均不高;圈养大熊猫口腔中有链球菌属(Streptococcus)、放线菌属(Actinomyces)、卟啉单胞菌属(Porphyromonas)和葡萄球菌属(Staphylococcus)等致病菌,其中与龋齿相关的菌群相对丰度较高,这可能与圈养大熊猫摄入了较多的碳水化合物有关。