长链非编码RNA POT1-AS1在宫颈癌顺铂耐药中的作用研究

背景同步放化疗是晚期宫颈癌(cervical cancer,CC)的治疗方法,顺铂(cisplatin,CDDP)是目前临床化疗使用的主要药物之一,CDDP耐药的发生严重制约了其临床应用,因此针对CDDP耐药发生机制的研究对CC的治疗至关重要。目的探索长链非编码RNA(long non-coding RNA,lncRNA)POT1-AS1在宫颈癌CDDP耐药中的作用。方法基于TCGA数据库,分析POT1-AS1在CC组织及正常组织中的表达情况,并分析POT1-AS1的表达量与预后的关系。采用CCK-8实验评估CDDP处理后的耐药细胞系和亲本细胞系、siPOT1-AS1组和siNC组的活力并测定相应的半抑制浓度(the half maximal inhibitory concentration,IC50)。实时荧光定量PCR(quantitative real-time PCR,qPCR)检测POT1-AS1在耐药细胞系及相应亲本细胞系中的表达以及POT1-AS1表达与CDDP作用时间的相关性。克隆形成实验检测POT1-AS1对CDDP耐药细胞增殖情况的影响。PI/DAPI双染荧光用于评估POT1-AS1与CDDP诱导的细胞死亡的相关性。结果POT1-AS1在CC肿瘤组织中高表达,并与不良预后相关。耐药细胞系的细胞活力及IC50值显著高于亲本细胞系(P<0.001),POT1-AS1沉默组的细胞活力及IC50值显著低于阴性对照(negative control,NC)组(P<0.001),差异均有统计学意义。POT1-AS1在耐药细胞系中的表达显著高于亲本细胞系(P<0.001),且随着CDDP给药时间的延长而增加。沉默POT1-AS1后可显著抑制耐药细胞的增殖能力(P<0.01)。与NC组相比,POT1-AS1沉默组的CC耐药细胞死亡率显著增加(P<0.01),尤其是在加入CDDP作用后(P<0.01)。结论POT1-AS1与宫颈癌CDDP耐药密切相关,并通过抑制CDDP诱导的细胞死亡发挥促癌作用。

基于改进POT模型的混凝土浇筑仓温度双控指标拟定

基于小概率法或最大熵法拟定浇筑仓温度双控指标时对样本数据的极端值保留程度欠缺,而现有文献关于温度双控指标拟定所选取的典型龄期没有明确的物理意义。为避免混凝土浇筑仓温度超过容许最高温度,结合某混凝土坝在高温季节浇筑仓实测温度,采用POT模型拟定了混凝土浇筑仓在绝热温升半熟龄期下的温度双控指标。为了提高结果可靠性,选用基于3σ准则改进的阈值确定方法,并提出了一种改进的POT模型条件概率确定方法。工程实例分析表明:采用改进POT模型与典型小概率法拟定的温度双控指标较为接近,但由于POT模型重点考虑了极端条件下的浇筑仓温度,对极端事件概率下的估计更为准确,由此求出的温度双控指标更合理。此外,选取混凝土浇筑仓在绝热温升半熟龄期作为拟定温度双控指标的典型龄期更利于工程推广。

极值BMM模型与POT模型对面板堆石坝安全监控指标拟定的比较研究

研究极值理论中极值BMM模型和POT模型在大坝安全监控指标拟定上的运用,通过引用经济学上的分位数和极端风险(VaR)值的概念,根据对应公式计算出相应数值进行量化比较分析。研究结果表明,极值BMM模型和POT模型在高置信水平下均能反映出数据的极端风险状况;在99%置信水平下两模型VaR值均大于在95%置信水平下的VaR值;在99%置信水平下极值BMM模型计算出的VaR值比POT模型下的VaR值更大,且接近于拟定指标,表明极值BMM模型下VaR值更能反映出数据的极值状态;在指标拟定方面,极值BMM模型较为适合拟定数据范围内的警戒指标,而POT模型指标拟定结果是超越样本数据本身,具有一定的预测性,适合拟定为样本数据的预测指标。

Study on a Bowl-based Mechanism for Transplanting Potted Strawberry Seedlings

To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to achieve rapid fetching and pushing bowl movements.According to the working principle of the slewing mechanism,a kinematics model and the optimization goal were established,respectively.Based on visual auxiliary analysis software,optimal parameters were obtained.A three-dimensional model was established to obtain a simulation trajectory by means of a virtual simulation design analysis.Three-dimensional printing technology was used to manufacture the test prototype,and the actual working trajectories of the test prototype were extracted using high-speed photography technology,which verified the consistency of the actual trajectory with the theoretical and simulated trajectories.A prototype transplanting experiment was performed with the success rate of seedling extraction of 91.2%and excellent planting rate of 82.8%,which met the requirements for integrated strawberry harvesting,planting and transplanting.The experimental results verified the correctness and feasibility of the design of integrated transplanting mechanism.

The optimal drainage line of potted flowers of soilless culture

Water is the source of life. China ranks No.13 among the water shortage countries. The most of the water is utilized in agriculture, while the utilization rate of irrigation water is only 40%, so it is very important to study on the theory and technology on high efficient water utilization. The purpose for this study is to find out the optimal drainage line on potted flowers through the analysis of experimental results of Aglaonema modestum and Rhododendron simsii Planch in different drainage lines.

Research on Potted-Tray Grown Rice Seedling Transplanting by Machine

[Objective] In order to cultivate seedlings that are adaptable to farming system and transplanter, the technology for transplanting potted-tray grown rice seedlings with machine was introduced and studied. [Method] The transplanter-spe-cific seedlings were cultivated with potted-tray, bowl-shaped blanket tray and soft plastic flat plate respectively. The seeding rate and 20-50-day-old seedling height were investigated for the 3 sowing patterns. After the transplanting, the missing transplanting rate, upright seedling rate, seedling injury rate and green-returning pe-riod were investigated. [Result] Nursing seedlings with potted-tray had the advan-tages of saving seeds, high sowing precision, high seedling rate and high seedling rate. The 20-40-day-old seedlings had relatively high leaf age, seedling height and root number. However, 40 d after the transplanting, the growth of seedlings was slowed. The quality difference was smal est among the 20-day-old seedlings that were nursed with 3 different patterns, but since then the quality advantage of pot-ted-tray grown seedlings had been more and more prominent. There was smal dif-ference in quality among the 30-day-old seedlings. However, for the seedlings that were older than 40 d, the transplanting quality of potted-tray grown seedlings was higher than that of bowl-shaped blanket tray grown seedlings and soft plastic flat plate grown seedlings. In addition, the green-returning period of the potted-tray grown seedlings was significantly shortened. [Conclusion] Compared to the bowl-shaped blanket tray grown seedlings and soft plastic flat plate grown seedlings, the potted-tray grown seedlings were more suitable to transplant by machine in the wheat-rice double cropping areas of Chengdu Plain.

Preliminary Study on the Accumulation Characteristics of Mineral Elements in Potted Poa crymophila under Drought and Water Stress

[Objective] This study was conducted to explain the mechanism of the accumulation characteristics of mineral elements in alpine grassland plants. [Method] Cultivated alpine grassland plant, Poa crymophila, was treated with drought and wa- ter stress, and then the samples were collected and analyzed. [Result] Compared with the control group, under drought and water stress, multiple mineral elements tended to accumulate and increase, and there were significant differences in the contents of Cu, Mn, Ni and P （P〈0.05）. [Conclusion] Under drought and water stress, mineral elements in potted Poa crymophila tended to accumulate and in- crease, which is the adaption and response of Poa crymophila to drought and water stress, as well as the re-verification of the starvation effect hypothesis of mineral effects. The starvation effect of mineral elements is one of the endogenic forces for the accumulation and differentiation of mineral elements in grassland plants.

高层建筑围护结构风压系数的概率特征及其极值POT估计

为获得高层建筑围护结构设计风荷载,通常需要考虑其表面风压系数的概率特征,进而进行极值估计。针对当前基于超越阈值模型的风压系数极值估计方法存在阈值选取困难,需要较大样本的不足,基于高层建筑标准模型进行风洞试验,首先研究其表面风压系数的概率特征,结果表明迎风区测点接近高斯分布,分离区测点风压系数母体接近Gamma分布,风压系数极小值接近GEV(general extreme value,GEV)分布;提出一种改进的POT(peak over threshold,POT)极值估计方法进行表面风压系数极值估计,进而与几种传统极值估计方法进行对比,结果表明改进POT极值估计方法可实现小样本的风压系数极值估计,其估计结果与大样本容量的标准极值偏差小于5%,且稳定性较好;最后给出了标准高层建筑模型表面极值风压系数。

基于POT模型的股票市场风险度量研究

股票市场风险度量是规避股市风险的关键所在,文章基于广义帕累托分布的POT模型对股票市场风险度量进行了研究。针对POT模型中阈值确定困难的问题,在对峰度法、Hill估计法分析的基础上,提出了斜率变点检测的阈值确定方法。研究发现,将POT模型应用于股票市场分析中,95%CI与90%CI相比,VaR更具可信度,且股市的流动性较低,其市场风险也就越大。

基于2σ-chauvenet准则的POT模型的膨胀土渠坡位移监控指标拟定

在深挖方膨胀土渠坡的长期服役过程中,借助位移监控指标实时辨识渠坡服役性态是确保渠坡安全运行的重要手段,因此拟定合理的监控指标对于保障渠坡工程的安全具有重要意义。在POT模型的基础上,引入2σ准则和chauvenet准则,建立了阈值与监控指标之间的对应关系,通过求解满足该对应关系下的最佳阈值,建立了膨胀土渠坡位移监控指标拟定的改进POT模型,并应用于南水北调中线工程陶岔段的深挖方膨胀土渠坡工程中。结果表明,相较传统POT模型,基于2σ-chauvenet准则的改进POT模型可有效避免主观性与随机误差,具有更高的计算精度,拟定的位移监控指标更偏于安全,对防范渠坡风险、确保渠坡安全长效运行具有更强的指导意义。

Required amounts of medium and fertilizer for potted culture of zucchini

In Japan, zucchini culture has yet to get underway, and the current costs of zucchini can be attributed to damage from soil-borne disease and the unstable yields due to seasonal change of female flowers. Eradication of these problems will lead to stable supply and a consequent price reduction of zucchini fruits. We previously clarified the efficacy of potted culture as a new culture method for zucchini, but potted culture can be burdensome as the weak water and nutrient retention capacity of the medium warrants its regular replacement. To solve this problem, in this study, we investigated the blend ratio for mixing rice husk charcoal with peat and the amount of fertilizer required for potted culture of zucchini. Results revealed no significant differences in the length of the largest leaf, total number of flowers, number of female flowers, and the ratio of female flowers to total flowers with different blend ratios of rice husk charcoal to peat. However, the number of harvested fruits increased with higher ratios of rice husk charcoal to peat and was highest at 80:20. The length of the largest leaf increased with increased amounts of fertilizer, with the best response was at 200 g. No significant differences were noted between the ratio of female flowers to total flowers in any treatments. In addition, the number of harvested fruits was highest with 160 g and 200 g of fertilizer. Taken together, the optimal blend ratio of rice husk charcoal to peat is 80:20, and the optimal amount of fertilizer with this ratio is 160 g.

基于POT模型的泄水闸检修门库裂缝开合度安全监控指标拟定

针对传统混凝土裂缝开合度安全监控指标拟定中对监测数据利用率不高,对数据尾部特征刻画差,导致拟定监控指标精度低的问题,本文结合王甫洲水利枢纽泄水闸检修门库近一年的裂缝实测资料,首先选取涉及汛期、高低温季节等不利工况作用下的典型裂缝开合度监测资料系列作为样本数据,然后引入POT模型拟定了裂缝开合度监控指标,最后对所拟定的裂缝开合度监控指标进行分析评价.结果表明,相对于典型小概率法拟定的监控指标,由于POT模型具有对采集信息利用率高、能够更好地刻画数据分布的尾部特征的优点,该方法拟定的裂缝开合度监控指标精度更高.

基于POT和BMM模型的甘河洪水演变特征分析

全球气候变化引起极端洪水频发和强度增大,增加流域洪水风险。甘河是嫩江尼尔基水库上游最大的支流,其洪水演变关乎下游尼尔基水库的防洪调度。本研究基于甘河1958~2018年的逐日气温、降水和径流等数据,利用块最大值模型和超阈值模型,在对洪水事件提取的基础上,分析了洪水事件发生次数、发生时间和洪峰流量的演变特征,并探究了洪水演变与降水、气温关系。结果表明:甘河流域洪水频发,61 a间共发生了124次洪水事件,且主要集中在5~10月。年最大洪峰流量总体呈增加趋势,增加速率为4.6 m^(3)/(s·a);年最大洪峰发生时间均有提前趋势,增加速率为0.37 d/a。洪水与气温相关性较弱,与降水密切相关,甘河流域易形成暴雨型洪水;洪峰发生前10 d的降水频率和强度与洪水的相关性最为密切,且年最大7日洪量和最大7日降水量变化趋势大致相当。研究成果可为甘河洪水风险管理和下游尼尔基水库的防洪调度与水安全保障提供参考。

Effects of Exogenous Phenolic Acids on Allelopathy of Potted Soybean Seedlings

In this study, several general y reported exogenous phenolic acids were selected as regents for potting experiments, aiming at revealing the effects of differ-ent concentrations of exogenous phenolic acids on the growth and development of soybean seedlings. The growth and development indicators of soybean seedlings treated with five different concentrations of exogenous phenolic acids, such as plant height, leaf area, root nodule number and root-shoot ratio, were determined and compared. According to the experimental results, three exogenous phenolic acids with different concentrations exhibited slight effects on plant height of potted soy-bean seedlings. The leaf area of potted soybean seedlings treated with 1.0 g/kg benzoic acid and cinnamic acid varied extremely significantly compared with the control. Root nodule number of potted soybean seedlings treated with 1.0 g/kg ben-zoic acid and vanil ic acid increased remarkably, but 1.0 g/kg cinnamic acid had lit-tle effects on root nodule number. When the concentration of phenolic acids was 1.0 g/kg, three phenolic acids exhibited the maximum effects on root-shoot ratio of potted soybean seedlings. Overal , low concentrations of exogenous phenolic acids exhibited no significant effects on the growth and development of potted soybean seedlings; high concentrations of exogenous phenolic acids inhibited the growth and development of potted soybean shoots, increased root nodule number and improved root-shoot ratio.

Effect of Spraying Liquid Mineral Mixed Fertilizers on the Mineral Elements of Potted Poa crymophila cv. Qinghai

Objective] The aim was to explain the accumulation characteristic of mineral elements in alpine grassland plants and the effect of supplementary supply on the nutrient changes of mineral elements. [Method] Potted Poa crymophila cv. Qinghai plants were regularly applied with liquid mineral mixed fertilizers, and samples were collected for analysis and detection. [Result] After the regular spraying of liquid mineral mixed fertilizers, the mineral elements in potted P. crymophila and its soil reduced （ P 〈0.05）, but the accumulation of corresponding mineral elements of P. crymophila in the control group was increased due to the ＂Starvation Effect＂, which was one of the endogenetic forces driving the increase in the accumulation of mineral elements. [Conclusion] This study is of great significance for the study of the accumulation of mineral elements in degraded grassland plants and its dynamic mechanism, as well as testing the hypothesis of the Starvation Effect of mineral elements. In addition, it also provides scientific bases and technical support for the restoration and remediation of degraded grassland and the development of ecological livestock husbandry.

Determination of Monitoring Control Value for Concrete Gravity Dam Spatial Deformation Based on POT Model

Deformation can directly reflect the working behavior of the dam,so determining the deformation monitoring control value can effectively monitor the safety of dam operation.The traditional dam deformation monitoring control value only considers the single measuring point.In order to overcome the limitation,this paper presents a new method to determine the monitoring control value for concrete gravity dam based on the deformations of multi-measuring points.A dam’s comprehensive deformation displacement is determined by the measured values at different measuring points on the positive inverted vertical line and the corresponding weight of eachmeasuring point.The projection pursuit method(PPM)combined with the grey wolf optimization(GWO)algorithm is used to determine the weight of each measuring point according to the spatial correlation distribution characteristics of dam deformation.The peaks over threshold(POT)model based on the extreme value theory is adopted to determine the monitoring control value with the obtained dam comprehensive deformation displacement.In addition,the POTmodel is improved with the automatic threshold determinationmethod based on the 3σcriterion in probability theory and the GWO algorithm,which can avoid subjectivity and randomness in determining the threshold.The results of the engineering application show the feasibility and applicability of the proposed method.

Elaboration of Ceramic Pot Filter from Kaolinite (Cameroon Clay) for the Elimination of Suspended Particles from Domestic Drinking Water

The objective of this work was to elaborate ceramic water filters from Kaolinite (Cameroon) clay for the elimination of suspended particles from domestic drinking water. In Sub-Sahara Africa and in Cameroon in particular health issues have been linked to the consummation of domestic tap water of high turbidity values both in the rural and urban areas. In order to remedy these problems, ceramic water pot filters have been elaborated in a pilot scale unit with aim of putting in place a unit production. The chemical composition, the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analyses of the raw materials (clay and rice husks) was determined. The crystal phases and scanning electron microscope of Wack clay was also determined. The ceramic pot filter membranes were fabricated from the formulations 70/20/10 of clay/porogen/chamotte respectively with the particle size of the raw material less than or equal to 500 μm. The formulated ceramic pot filters were then sintered at 900˚C in a furnace. These ceramic pot filters were characterized by determining their porosity, withdrawal percentages, water permeability, mechanical and chemical resistance. The study of the efficiency consisted in evaluating the retention rate and permeate flux with respect to time (days) with synthetic water suspensions of turbidity 100 NTU and particle size of 2 μm. The ceramic pot filters were made aiming at studying the efficiency after physical defouling of filters. Physical defouling consisted in brushing the inner surface of the ceramic pot filters with water and drying them at ambient temperature after being used for 11 days and reusing them under the same initial conditions. The produced ceramic pot filter had a volume of 4 L, an average porosity of 36.15%, shrinkage in mass or withdrawal percentage of 18.23%, a water permeability of 59.6 × 103 L∙h−2∙m−2, mechanical resistance of 6.8 MPa and corrosion resistance of 1.6% in acidic medium and 0.8% in alkaline medium. The evaluation of the retention efficiency reveals that the retention rate of 99.9% was obtained from the 9th day of filtration reducing the turbidity value from 100 NTU to less than 0.1 NTU. From the filtration test carried out during the 11 consecutive days, the flow rate varied between 1.46 L∙h−1 to 2.63 L∙h−1. Similar results of retention and flow rate were obtained after physical defouling of the ceramic pot filter membranes and re-using for 11 consecutive days, showing the efficiency of the ceramic pot filter membranes in eliminating suspended particles from drinking water. Cost evaluation for the production unit reveals a total cost of production for 50 ceramic pot filters of 1593.6 USD consisting of fixed assets and variable assets. An estimated selling price of 3.3 USD was obtained which is affordable for both the urban and rural population in Cameroon and in sub-Saharan Africa.

番茄钵苗移栽探出式取钵机构设计与试验

为了降低番茄钵苗移栽过程取钵机构对秧苗钵土根系的损伤,同时避免机械式钵苗移栽机构设计特殊取苗轨迹与姿态的优化难题,提出了一种可与系列移栽机构配合使用的番茄钵苗探出式取钵机构,实现取苗各关键位置机构秧针以固定角度完成探出入钵、移动送苗及收回推秧工序。根据钵苗移栽取钵过程分析与设计要求,建立了探出式取钵机构力学分析模型,并获得影响秧针扎入钵土时驱动杆受最小驱动力的因素。基于Matlab App Designer平台开发了取钵机构计算机辅助分析设计软件,获得满足番茄钵苗移栽要求的取钵机构设计参数集。采用三因素五水平二次回归正交旋转中心组合试验方法,以驱动杆斜杆夹角、钵体含水率、入钵深度为试验因素,以钵体完整率和取苗成功率为评价指标,试制样机并搭建台架实施参数组合优化及验证试验,结果表明:探出式取钵机构可有效地配合取苗机构完成各项性能工作要求,在参数组合为驱动斜杆间夹角112°、钵体含水率57.5%、入钵深度28.4 mm时作业效果最佳,钵体完整率为96.44%,取苗成功率为97.06%,满足钵苗移栽作业性能。

Elaboration of Ceramic Pot Filter from Kaolinite (Cameroon Clay) for the Elimination of Suspended Particles from Domestic Drinking Water

The objective of this work was to elaborate ceramic water filters from Kaolinite (Cameroon) clay for the elimination of suspended particles from domestic drinking water. In Sub-Sahara Africa and in Cameroon in particular health issues have been linked to the consummation of domestic tap water of high turbidity values both in the rural and urban areas. In order to remedy these problems, ceramic water pot filters have been elaborated in a pilot scale unit with aim of putting in place a unit production. The chemical composition, the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analyses of the raw materials (clay and rice husks) was determined. The crystal phases and scanning electron microscope of Wack clay was also determined. The ceramic pot filter membranes were fabricated from the formulations 70/20/10 of clay/porogen/chamotte respectively with the particle size of the raw material less than or equal to 500 μm. The formulated ceramic pot filters were then sintered at 900˚C in a furnace. These ceramic pot filters were characterized by determining their porosity, withdrawal percentages, water permeability, mechanical and chemical resistance. The study of the efficiency consisted in evaluating the retention rate and permeate flux with respect to time (days) with synthetic water suspensions of turbidity 100 NTU and particle size of 2 μm. The ceramic pot filters were made aiming at studying the efficiency after physical defouling of filters. Physical defouling consisted in brushing the inner surface of the ceramic pot filters with water and drying them at ambient temperature after being used for 11 days and reusing them under the same initial conditions. The produced ceramic pot filter had a volume of 4 L, an average porosity of 36.15%, shrinkage in mass or withdrawal percentage of 18.23%, a water permeability of 59.6 × 103 L∙h−2∙m−2, mechanical resistance of 6.8 MPa and corrosion resistance of 1.6% in acidic medium and 0.8% in alkaline medium. The evaluation of the retention efficiency reveals that the retention rate of 99.9% was obtained from the 9th day of filtration reducing the turbidity value from 100 NTU to less than 0.1 NTU. From the filtration test carried out during the 11 consecutive days, the flow rate varied between 1.46 L∙h−1 to 2.63 L∙h−1. Similar results of retention and flow rate were obtained after physical defouling of the ceramic pot filter membranes and re-using for 11 consecutive days, showing the efficiency of the ceramic pot filter membranes in eliminating suspended particles from drinking water. Cost evaluation for the production unit reveals a total cost of production for 50 ceramic pot filters of 1593.6 USD consisting of fixed assets and variable assets. An estimated selling price of 3.3 USD was obtained which is affordable for both the urban and rural population in Cameroon and in sub-Saharan Africa.