A water soluble crude polysaccharide has been isolated from the fruiting body of cultivated Cordyceps militarvs(L.) Link. The GC analysis indicated that it contained the monosaccharides of Gal, Man, Glc and GlcA. Thei...A water soluble crude polysaccharide has been isolated from the fruiting body of cultivated Cordyceps militarvs(L.) Link. The GC analysis indicated that it contained the monosaccharides of Gal, Man, Glc and GlcA. Their molar ratio is 1 00∶1 58∶7 89∶0 19. The crude polysaccharide was fractionated with ethanol to get three fractions, which were CM Ⅰ, CM Ⅱ and CM Ⅲ. CM Ⅱ was further purified to get one homogeneous fraction named CMB. The molecular weight was estimated to be 60000 by means of gel filtration. CMB is a greyish white powder. The GC analysis indicated that it was composed of Gal, Man, Glc and the molar ratio is 1 00∶1 38∶5 10. Partial hydrolysis with an acid, periodate oxidation, Smith degradation, IR, GC and methylation analyses, GC/MS analyses were used for the structure analyses of CMB. The results reveal that CMB is of a lower branched structure. The main chain is composed of (1→6) linked Man, (1→6) linked Glc, (1→4) linked Glc and (1→4) linked Gal. Some of (1→4) linked Glc and all (1→4) linked Gal residues are substituted at 3 O and 6 O respectively. (1→4) Linked Glc residues without branch probably exist with other monosaccharides alternately. On an average, there is one branch every ten hexose residues. The side chain is composed of major (1→4) linked Glc and minor (1→6) linked Glc. The nonreduced end is composed of Gal and Glc. The main glycosidic link of CMB is α configuration.展开更多
Pollen viability study on Piper colubrinum Link. carried out to develop interspecific hybridization between P. nigrum L. and P. colubrinum Link.. This plant has good potential as a donor plant in breeding programme fo...Pollen viability study on Piper colubrinum Link. carried out to develop interspecific hybridization between P. nigrum L. and P. colubrinum Link.. This plant has good potential as a donor plant in breeding programme for Phytophthora to foot rot resistance in the cultivated species, P. nigrum. This project was carried out to reveal the optimum time to collect pollen of P. colubrinum for artificial pollination. Through this study, anther dehiscence was proved occurred around 9 am. Based on the result obtained from study on the time of anther dehiscence, the pollen was classified into five stages for testing on the viability, i.e., stage 1, before anther dehiscence, i.e., around 7 am （pollen obtained by crushing on the anther）; stage 2： immediately after anther dehiscence, i.e., around 9 am; stage 3： two hours after anther dehiscence, i.e., around 11 am; stage 4： four hours after anther dehiscence, i.e., around 1 pm; and stage 5： six hours after anther dehiscence, i.e., around 3 pm. Data obtained from pollen germination and pollen tube growth study showed that pollen of P. colubrinum was the most viable at two hours after anther dehiscence and onward. It was also proved that the viability can be maintained up to at least eight hours. Pollen obtained before anther dehiscence by crushing on the anther was found less viable with lower percentage of pollen germination and retarded pollen tube growth. In other words, the experiment enlightened that artificial pollination between P. nigrum L. and P. colubrinum Link was ideally to be carded out approximately starting from 11 am until 5 pm, considering the viability of pollen ofP. colubrinum.展开更多
Multilayer film was fabricated on an electrode surface by alternate layer-by-layer（LBL） adsorption of polycationic redox polymer（PEI-Fc） and dye-linked L-proline dehydrogenase（L-proDH）.The electrochemistry of th...Multilayer film was fabricated on an electrode surface by alternate layer-by-layer（LBL） adsorption of polycationic redox polymer（PEI-Fc） and dye-linked L-proline dehydrogenase（L-proDH）.The electrochemistry of the PEI-Fc/L-proDH multilayer modified electrode was investigated by cyclic voltammetry,and the enzyme catalysis mediated by the redox polymer was studied in a solution containing L-proline.It was observed that electron communication between L-proDH and the electrode was achieved with the help of PEI-Fc.A mathematical expression for the current response was evaluated based on the Michaelis-Menten kinetics mode,and the calculated currents fitted well with the experimental data.The kinetic analysis indicates that only a small fraction of the immobilized enzyme was efficiently electrically wired by the redox polymer.展开更多
Marker-aided selection has received more attention in recent years. This relies on the exploitation of dose linkage between molecular markers and target gene(s). We report here a randomly amplified polymorphic DNA (RA...Marker-aided selection has received more attention in recent years. This relies on the exploitation of dose linkage between molecular markers and target gene(s). We report here a randomly amplified polymorphic DNA (RAPD) marker tightly linked to the blast resistance gene Pi-ll(t) derived from Hongjiaozhan, which confers the resistance to race ZB1 of Pyricularia oryzae Cav.展开更多
目的:研究小鼠风寒表证康复过程中IL-10的变化规律。方法:采用酶联免疫吸附实验(ELISA)法定量检测小鼠风寒表症动物疾病模型实验组和对照组1周内每日11时与23时的血清IL-10的表达水平。结果:与对照组相比,日间1～4 d IL-10水平上升,第3...目的:研究小鼠风寒表证康复过程中IL-10的变化规律。方法:采用酶联免疫吸附实验(ELISA)法定量检测小鼠风寒表症动物疾病模型实验组和对照组1周内每日11时与23时的血清IL-10的表达水平。结果:与对照组相比,日间1～4 d IL-10水平上升,第3d达高峰(P<0.01),以后逐渐下降;夜间2d后水平升高(P<0.01),5 d后水平逐渐下降。结论:小鼠风寒表证康复中IL-10的周期性变化表现为实验组水平高于对照组(P<0.01);对照组IL-10存在有昼低夜高的日节律变化,实验组日节律变化不明显,可能与免疫功能的介入与调控和疾病康复相关。展开更多
文摘A water soluble crude polysaccharide has been isolated from the fruiting body of cultivated Cordyceps militarvs(L.) Link. The GC analysis indicated that it contained the monosaccharides of Gal, Man, Glc and GlcA. Their molar ratio is 1 00∶1 58∶7 89∶0 19. The crude polysaccharide was fractionated with ethanol to get three fractions, which were CM Ⅰ, CM Ⅱ and CM Ⅲ. CM Ⅱ was further purified to get one homogeneous fraction named CMB. The molecular weight was estimated to be 60000 by means of gel filtration. CMB is a greyish white powder. The GC analysis indicated that it was composed of Gal, Man, Glc and the molar ratio is 1 00∶1 38∶5 10. Partial hydrolysis with an acid, periodate oxidation, Smith degradation, IR, GC and methylation analyses, GC/MS analyses were used for the structure analyses of CMB. The results reveal that CMB is of a lower branched structure. The main chain is composed of (1→6) linked Man, (1→6) linked Glc, (1→4) linked Glc and (1→4) linked Gal. Some of (1→4) linked Glc and all (1→4) linked Gal residues are substituted at 3 O and 6 O respectively. (1→4) Linked Glc residues without branch probably exist with other monosaccharides alternately. On an average, there is one branch every ten hexose residues. The side chain is composed of major (1→4) linked Glc and minor (1→6) linked Glc. The nonreduced end is composed of Gal and Glc. The main glycosidic link of CMB is α configuration.
文摘Pollen viability study on Piper colubrinum Link. carried out to develop interspecific hybridization between P. nigrum L. and P. colubrinum Link.. This plant has good potential as a donor plant in breeding programme for Phytophthora to foot rot resistance in the cultivated species, P. nigrum. This project was carried out to reveal the optimum time to collect pollen of P. colubrinum for artificial pollination. Through this study, anther dehiscence was proved occurred around 9 am. Based on the result obtained from study on the time of anther dehiscence, the pollen was classified into five stages for testing on the viability, i.e., stage 1, before anther dehiscence, i.e., around 7 am （pollen obtained by crushing on the anther）; stage 2： immediately after anther dehiscence, i.e., around 9 am; stage 3： two hours after anther dehiscence, i.e., around 11 am; stage 4： four hours after anther dehiscence, i.e., around 1 pm; and stage 5： six hours after anther dehiscence, i.e., around 3 pm. Data obtained from pollen germination and pollen tube growth study showed that pollen of P. colubrinum was the most viable at two hours after anther dehiscence and onward. It was also proved that the viability can be maintained up to at least eight hours. Pollen obtained before anther dehiscence by crushing on the anther was found less viable with lower percentage of pollen germination and retarded pollen tube growth. In other words, the experiment enlightened that artificial pollination between P. nigrum L. and P. colubrinum Link was ideally to be carded out approximately starting from 11 am until 5 pm, considering the viability of pollen ofP. colubrinum.
基金Supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars of the Ministry of Education,Chinathe Natural Science Foundation of Tianjin City,China(No.06YFJMJC15000)
文摘Multilayer film was fabricated on an electrode surface by alternate layer-by-layer（LBL） adsorption of polycationic redox polymer（PEI-Fc） and dye-linked L-proline dehydrogenase（L-proDH）.The electrochemistry of the PEI-Fc/L-proDH multilayer modified electrode was investigated by cyclic voltammetry,and the enzyme catalysis mediated by the redox polymer was studied in a solution containing L-proline.It was observed that electron communication between L-proDH and the electrode was achieved with the help of PEI-Fc.A mathematical expression for the current response was evaluated based on the Michaelis-Menten kinetics mode,and the calculated currents fitted well with the experimental data.The kinetic analysis indicates that only a small fraction of the immobilized enzyme was efficiently electrically wired by the redox polymer.
文摘Marker-aided selection has received more attention in recent years. This relies on the exploitation of dose linkage between molecular markers and target gene(s). We report here a randomly amplified polymorphic DNA (RAPD) marker tightly linked to the blast resistance gene Pi-ll(t) derived from Hongjiaozhan, which confers the resistance to race ZB1 of Pyricularia oryzae Cav.
文摘目的:研究小鼠风寒表证康复过程中IL-10的变化规律。方法:采用酶联免疫吸附实验(ELISA)法定量检测小鼠风寒表症动物疾病模型实验组和对照组1周内每日11时与23时的血清IL-10的表达水平。结果:与对照组相比,日间1～4 d IL-10水平上升,第3d达高峰(P<0.01),以后逐渐下降;夜间2d后水平升高(P<0.01),5 d后水平逐渐下降。结论:小鼠风寒表证康复中IL-10的周期性变化表现为实验组水平高于对照组(P<0.01);对照组IL-10存在有昼低夜高的日节律变化,实验组日节律变化不明显,可能与免疫功能的介入与调控和疾病康复相关。
