Amh (anti-Müllerian hormone) is a single copy gene which is expressed strongly in Sertoli cells in the foetal testis and participates in the onset of sexual differentiation. Its promoter driving the expression of...Amh (anti-Müllerian hormone) is a single copy gene which is expressed strongly in Sertoli cells in the foetal testis and participates in the onset of sexual differentiation. Its promoter driving the expression of a reporter gene (d2EGFP) has been used to analyse the role of certain defined putative elements and a downstream enhancer element in gene expression. These experiments were carried out in vitro using a line of pre-pubertal mouse Sertoli cells, transienly transfected with circular DNA constructs with variously mutated promoter elements. A downstream enhancer element, situated immediately 3’ of the polyadenylation (PA) signal for Amh, has been inserted in an equivalent position in the d2EGFP construct. When the Amh promoter is unmodified, the downstream enhancer (DE) is positively associated with a large increase in EGFP expression. This is at least partly the consequence of an increased rate of expression by individual cells. Experiments using variously truncated Amh promoters indicate that an upstream region (-214 to -336) may play a minor role in facilitating enhancement. However mutation of the Wilms tumour factor-1 element, situated between the tata box and the start of translation, results in an almost complete suppression of enhancement.展开更多
The situations of four major earthquakes occurred in Chongqing in modern times were analyzed in this paper.According to the study of the relationship between drought and earthquake and the study on five abnormal indic...The situations of four major earthquakes occurred in Chongqing in modern times were analyzed in this paper.According to the study of the relationship between drought and earthquake and the study on five abnormal indices of meteorological elements,the pre-earthquake abnormal meteorological elements of these earthquakes occurred in Tongjing Town of Yubei,Chongqing in 1989,Xuxi of Rongchang in Chongqing in 1997,Rongchang County of Chongqing in 1999 respectively were systemically tested.Finally,the assumption of establishing an earthquake warning system of abnormal meteorological elements in the whole nation,especially in some high-risk earthquake areas was proposed.展开更多
文摘Amh (anti-Müllerian hormone) is a single copy gene which is expressed strongly in Sertoli cells in the foetal testis and participates in the onset of sexual differentiation. Its promoter driving the expression of a reporter gene (d2EGFP) has been used to analyse the role of certain defined putative elements and a downstream enhancer element in gene expression. These experiments were carried out in vitro using a line of pre-pubertal mouse Sertoli cells, transienly transfected with circular DNA constructs with variously mutated promoter elements. A downstream enhancer element, situated immediately 3’ of the polyadenylation (PA) signal for Amh, has been inserted in an equivalent position in the d2EGFP construct. When the Amh promoter is unmodified, the downstream enhancer (DE) is positively associated with a large increase in EGFP expression. This is at least partly the consequence of an increased rate of expression by individual cells. Experiments using variously truncated Amh promoters indicate that an upstream region (-214 to -336) may play a minor role in facilitating enhancement. However mutation of the Wilms tumour factor-1 element, situated between the tata box and the start of translation, results in an almost complete suppression of enhancement.
文摘The situations of four major earthquakes occurred in Chongqing in modern times were analyzed in this paper.According to the study of the relationship between drought and earthquake and the study on five abnormal indices of meteorological elements,the pre-earthquake abnormal meteorological elements of these earthquakes occurred in Tongjing Town of Yubei,Chongqing in 1989,Xuxi of Rongchang in Chongqing in 1997,Rongchang County of Chongqing in 1999 respectively were systemically tested.Finally,the assumption of establishing an earthquake warning system of abnormal meteorological elements in the whole nation,especially in some high-risk earthquake areas was proposed.
