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Analysis of Monosaccharide Composition of Pu-erh Tea Polysaccaride by Pre-column Derivatization HPLC 被引量:2
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作者 郭威 周斌星 +4 位作者 罗玲 李扬 柴洁 杨超 马存强 《Agricultural Science & Technology》 CAS 2013年第4期556-558,572,共4页
[Objective] This study aimed to establish a pre-column derivatization HPLC method for the identification and analysis of monosaccharide composition of Pu-erh tea polysaccharide. [Method] Pu-erh tea polysaccharide was ... [Objective] This study aimed to establish a pre-column derivatization HPLC method for the identification and analysis of monosaccharide composition of Pu-erh tea polysaccharide. [Method] Pu-erh tea polysaccharide was extracted using the wa- ter extraction method, further isolated and purified by DEAE cellulose-52 columns. The obtained tea polysaccharide and four components TPS1, TPS2, TPS3 and TPS, were first derived by 1-phenyl-3-methyl-5-pyrazolone (PMP), and then the PMP derivatives of monosaccharide were analyzed by high performance liquid chromatog- raphy (HPLC). [Result] Pu-erh tea polysaccharide contained eight kinds of monosac- chaddes (mannose, rhamnose, glucuronic acid, galacturonic acid, grucose, galactose, arabinose, fucose), without xylose; so it was the same with TPS1; each of TPS2, TPS3 and TPS4 contained seven monosaccharides, while no fucose. [Conclusion] This method is simplified and rapid, which can be used to determine the monosac- charide composition of Pu-erh tea polysaccharide and monosaccharide content. 展开更多
关键词 Pu-erh tea POLYSACCHARIDE HPLC pre-column derivatization
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Ionic Group Derivitized Nano Porous Carbon Electrodes for Capacitive Deionization 被引量:4
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作者 Marc Andelman 《Journal of Materials Science and Chemical Engineering》 2014年第3期16-22,共7页
Capacitance for electrostatic adsorption forms primarily within a Debye length of the electrode surface. Capacitive carbon electrodes were derivatized with ionic groups by means of adsorbing a surfactant in order to t... Capacitance for electrostatic adsorption forms primarily within a Debye length of the electrode surface. Capacitive carbon electrodes were derivatized with ionic groups by means of adsorbing a surfactant in order to test the theory that attached ionic groups would exclude co-ions and increase coulombic efficiency without the need for an added charge barrier membrane. It has been discovered that capacitive electrodes surface derivatized with ionic groups become polarized and intrinsically more coulombically efficient. 展开更多
关键词 Capacitive DEIONIZATION Flow Through Capacitor Polarized Electrode NANOPOROUS CARBON derivitized CARBON ELECTRODES DEIONIZATION DESALINATION Water Purification IONIC Groups
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Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization 被引量:1
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作者 Li Ming DU Zhe Feng FAN +1 位作者 Jin Li QIAO Jing Ping WANG 《Chinese Chemical Letters》 SCIE CAS CSCD 2001年第11期1007-1010,共4页
Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new met... Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%similar to 95.7% and the RSD is 1.2%similar to4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine. 展开更多
关键词 SPARFLOXACIN HPLC pre-column derivatization human urine
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Determination of 18 Kinds of Amino Acids in Fresh Tea Leaves by HPLC Coupled with Pre-column Derivatization 被引量:2
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作者 Shangwen DONG Tengfei LIU Minghui DONG 《Asian Agricultural Research》 2018年第2期55-58,63,共5页
A rapid and accurate quantitative method of high performance liquid chromatography( HPLC) with fluorescence detector has been developed for the analysis of 18 kinds of amino acids in fresh tea leaves. The samples were... A rapid and accurate quantitative method of high performance liquid chromatography( HPLC) with fluorescence detector has been developed for the analysis of 18 kinds of amino acids in fresh tea leaves. The samples were minced and mixed,and extracted with ultra pure water at 90℃ for 20 min. The 6-aminoquinolyl N-hydroxy-succinimidyl carbamate( AQC) was used as pre-column derivatization reagent. Gradient HPLC separation was performed on a C_(18) column( Symmetry C_(18),3. 9 mm × 15 cm,4 μm). Good linearity between concentrations and peak areas was achieved in the concentration range of 5. 0-250 μmol/L for 18 kinds of amino acids. The method was validated by the analysis of five replicates. The 18 kinds of amino acid standards were spiked in fresh tea leaf samples and the average recovery rate was 86. 25%-109. 05% with relative standard deviations( n = 5) ranging from 6. 03% to 10. 56%. The limit of detection( LOD) for the analytes was0. 05-1. 27 μmol/L. The method was successfully applied to the analysis of the 18 kinds of amino acids in fresh tea leaves from east Dongting and west Dongting mountains in Suzhou. The results indicate that the method is simple,rapid,precise and reliable. 展开更多
关键词 Fresh tea leaves Free amino acids pre-column derivatization High performance liquid chromatography(HPLC)
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Preparative Resolution of Gatifloxacin Enantiomers with Pre-Column Esterification Strategy and Comparing Their Enantioselectivity to Bacteria and Antibody 被引量:1
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作者 ZHANG Chi Jian LEI Hong Tao +4 位作者 HUANG Xiao Long LIU Ying Ju CAI Kai XU Zhen Lin SUN Yuan Ming 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第2期157-160,共4页
Gatifloxacin (GFX) is a kind of chiral fluoroquinolones compound due to the methyl group at the C-3 position of the piperazine ring[1]. Although the enantiomers of GFX show similar levels of antimicrobial activity a... Gatifloxacin (GFX) is a kind of chiral fluoroquinolones compound due to the methyl group at the C-3 position of the piperazine ring[1]. Although the enantiomers of GFX show similar levels of antimicrobial activity and pharmacokinetics[2], the other biological activities (i.e., toxicity or enantioselective recognition to various receptors in vivo) of GFX enantiomers have not yet been studied. With this in mind, we developed a rapid and cost-effective high performance liquid chromatographic (HPLC) separation procedure for GFX enantiomers with a pre-column esterification strategy. 展开更多
关键词 Preparative Resolution of Gatifloxacin Enantiomers with pre-column Esterification Strategy and Comparing Their Enantioselectivity to Bacteria and Antibody DEA than
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Enantioresolution of a Series of Chiral Benzyl Alcohols by HPLC on a Dinitrobenzoylphenylglycine Stationary Phase after Achiral Pre-Column Derivatization
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作者 Svilen P. Simeonov Anton P. Simeonov +1 位作者 Aleksandar R. Todorov Vanya B. Kurteva 《American Journal of Analytical Chemistry》 2010年第1期1-13,共13页
High performance liquid chromatography method for the separation of a series of chiral benzyl alcohols on N-(3,5-dinitrobenzoyl)-D-phenylglycine stationary phase (Macherey Nagel, Chiral-2) after pre-column achiral der... High performance liquid chromatography method for the separation of a series of chiral benzyl alcohols on N-(3,5-dinitrobenzoyl)-D-phenylglycine stationary phase (Macherey Nagel, Chiral-2) after pre-column achiral derivatization was developed. Cheap and easy available aromatic acid chlorides were used as derivatization agents. Good to excellent separations of the enantiomers were achieved in all cases in relatively short analytical runs. It was shown that the enantiorecognition depends on the substituents both in the starting alcohol and in the acid chloride. The method presents an efficient alternative to the direct analyses on polysaccharide and cyclodextrine-derived stationary phases. 展开更多
关键词 HPLC DNBPG ENANTIOSEPARATION BENZYL ALCOHOLS Achiral pre-column DERIVATIZATION BENZOATES Chlorobenzoates Naphthoates
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Pre-column Derivatization-High Performance Liquid Chromatography for the Detection of Monensin in Livestock and Poultry Meat
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作者 Jiao WANG Xiujuan WANG +2 位作者 Lingtong HU Guixia YANG Dandan HU 《Agricultural Biotechnology》 CAS 2021年第2期93-94,共2页
[Objectives]A method for the detection of monensin in poultry and livestock meat by pre-column derivatization-high performance liquid chromatography was established.[Methods]The sample was extracted with chloroform,de... [Objectives]A method for the detection of monensin in poultry and livestock meat by pre-column derivatization-high performance liquid chromatography was established.[Methods]The sample was extracted with chloroform,derivatized with trichloroacetic acid and 2,4-dinitrophenylhydrazine,and centrifuged to obtain a purified solution.A C18 chromatographic column(4.6 mm×150 mm,5μm)was used for separation with(1.5%)acetic acid water∶methanol(volume ratio)=1∶9 as the mobile phase using a DAD detector for detection,and the external standard method was adopted for peak area quantification.[Results]Monensin had good linearity in the concentration range of 5.00-200 mg/L,with the linear correlation coefficient r 2>0.999;the detection limit was 5.00 mg/kg;the relative standard deviation was smaller than 10%;and the recoveries of standard addition experiment were in the range of 75%-110%.[Conclusions]The method has the advantages of simple pretreatment operation,good derivatization effect and fast detection speed,and is suitable for detecting monensin in poultry and livestock meat. 展开更多
关键词 pre-column derivatization-high performance liquid chromatography Livestock and poultry meat MONENSIN
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The Fate of Chromium in Weathered Ultramafic Rocks and Their Derivitative Soils in Cuba: Clues from Spectroscopic Studies
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作者 Andrea L.FOSTER 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2017年第S1期12-,共1页
In its cationic,trivalent form,Chromium(Cr)it is a micronutrient,and exhibits low environmental mobility.In hexavalent form,however,it is a human carcinogen and also highly mobile.Climate is a key environmental factor... In its cationic,trivalent form,Chromium(Cr)it is a micronutrient,and exhibits low environmental mobility.In hexavalent form,however,it is a human carcinogen and also highly mobile.Climate is a key environmental factor controlling weathering rates and stability of primary and secondary Cr-bearing minerals.Knowledge of Cr oxidation state and mineral residence is therefore essential to estimating the risk posed by Cr in serpentinites,chromite mine wastes,and soils developed on these parent materials.X-ray absorption spectroscopy(XAS)is currently the best available technique for determination of the relative abundance of Cr(III)and Cr(VI)in situ(that is,without digestion of solid phases).A brief review of relevant XAS studies of is presented below,focusing on studies in tropical climates1,as they will be most relevant to eastern Cuba’s extensively serpentinized ophiolite belt.Cr(III)-bearing spinels are usually the dominant and most refractory Cr host in ultramafic rocks.Previous XAS studies2 indicate that in tropical climates,Cr-spinels weather rapidly to form Cr(III)-bearing secondary Fe(III)(hydr)oxides(goethite,hematite).Manganese(Mn)is also enriched in ultramafic rocks2;as Mn(IV),it can also co-precipitate with Fe(III)(hydr)oxides,or form its own secondary(hydr)oxides.A previous study found up to 20%Cr(VI)in in a tropical,serpentine soil that contained substantial Mn,and a strong correlation between the*amounts of Cr(VI)and Mn(IV)in the soil profile2.Theresults of several XAS studies suggest that a close association of Mn(IV)and Cr(III)in secondary Fe(hydr)oxides is necessary for oxidation of Cr(III)to Cr(VI)via electron transfer reactions with Mn(IV);however,additional XAS studies have shown that organic matter3and Cr-bearing aluminosilicates4 may also be important sources of Cr(III)to the environment under specific conditions.The stability and fate of Cr has not been studied in detail for these two host phase types,to the best of our knowledge.Access to XAS facilities to perform Cr geochemical experiments is limited and will only become more so in the future.We are working to develop and apply(micro)Raman spectroscopy to evaluate Cr oxidation state and mineral residence(in crystalline and amorphous materials).In addition to standard Raman scattering,we are employing resonance Raman(785 nm laser)to enhance signal from Cr(VI)-bearing phases and laser-stimulated photoluminescence to identify Cr(III)associated with Al-rich alteration products 展开更多
关键词 Cr Clues from Spectroscopic Studies The Fate of Chromium in Weathered Ultramafic Rocks and Their derivitative Soils in Cuba
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Coupling of on-Line Pre-Column Oxidative Cleavage and Solid-Phase Enrichment with Liquid Chromatography Using an Eco-Friendly Analytical Procedure to Determine Low Levels of Methotrexate
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作者 Samy Emara Walaa Zarad +1 位作者 Maha Kamal Ramzia EL-Bagary 《Journal of Analytical Sciences, Methods and Instrumentation》 2012年第4期194-202,共9页
A simple, sensitive and precise green high-performance liquid chromatographic method including on-line pre-column oxidation combined by column switching with a short Hypersil ODS analytical column (100 mm × 4.0 m... A simple, sensitive and precise green high-performance liquid chromatographic method including on-line pre-column oxidation combined by column switching with a short Hypersil ODS analytical column (100 mm × 4.0 mm i.d.) for enrichment and separation was developed and validated to determine low levels of methotrexate (MTX). The method was based on oxidative cleavage of MTX into highly fluorescence products, 2,4-diaminopteridine-6-carboxaldehyde and the corresponding 2,4-diaminopteridine-6-carboxylic acid, during the flow of phosphate buffer (0.04 M, pH 3.4) containing the analyte through the packed reactor of cerium (IV) trihydroxyhydroperoxide (CTH) at a flow-rate of 0.2 mL/min and 40℃. The fluorescent products were enriched on the head of ODS analytical column for the final separation. The separation was performed at room temperature using an environmentally friendly mobile phase consisting of ethanol and phosphate buffer (0.04 M, pH 3.4) in the ratio of 10:90 (v/v). The eluent was monitored at emission and excitation wavelengths of 463 and 367 nm, respectively. The method was successfully applied, without any interference from the excipients, for the determination of drug in tablets and vials with a detection limit of 0.06 ng/mL from 500 ?L of sample MTX. 展开更多
关键词 Cerium (IV) Trihydroxyhydroperoxide HPLC METHOTREXATE ODS ANALYTICAL Column ON-LINE pre-column Oxidative CLEAVAGE
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HPLC of Amino Acids and Oligopeptides by Pre-Column Fluorescence Derivatization with 9-Acridine Formyl Chloride
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作者 Jin Mao YOU Xin Jun FAN Qing Yu OU(Lanzhou Institute of Chemical Physics. Chinese Academy of Sciences 730000) 《Chinese Chemical Letters》 SCIE CAS CSCD 1997年第10期875-878,共4页
A highly sensitive HPLC method for the detection of amino acids and oligopeptides with 9-acridine formyl chloride by pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, histidine, trigly... A highly sensitive HPLC method for the detection of amino acids and oligopeptides with 9-acridine formyl chloride by pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, histidine, triglycine and glutathione were separated on a reversed-phase C-18 column with methanol-water-triethylamine eluent, derivatization and chromatographic conditions were optimized. The five derivatives were eluted in 28 min with a good reproducibility. Linear range of the calibration graph was 0.08-260 nmol/ml(-1). The relative standard deviations(n=6) are < 5%. Detection limits (signal-to-noise ratio=3) for the five derivatives are 20-40 fmol. 展开更多
关键词 HPLC of Amino Acids and Oligopeptides by pre-column Fluorescence Derivatization with 9-Acridine Formyl Chloride
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柱前衍生非水毛细管电泳分离钴、镍和铜 被引量:6
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作者 刘笔锋 刘良斌 程介克 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 1998年第8期1232-1235,共4页
提出柱前衍生非水毛细管电泳分离金属离子的方法.应用新合成的2-(6-甲基-2-苯并噻唑偶氮)-5-二乙胺基酚作为柱前衍生试剂,乙醇和N,N-二甲基甲酰胺为非水溶剂,在6min内实现了钴、镍和铜的分离,灵敏度高,检测限... 提出柱前衍生非水毛细管电泳分离金属离子的方法.应用新合成的2-(6-甲基-2-苯并噻唑偶氮)-5-二乙胺基酚作为柱前衍生试剂,乙醇和N,N-二甲基甲酰胺为非水溶剂,在6min内实现了钴、镍和铜的分离,灵敏度高,检测限分别为9.18×10-8mol/L、2.79×10-7mol/L和4.47×10-7mol/L.考察了柱前衍生条件、非水溶剂配比以及分离参数对分离的影响. 展开更多
关键词 柱前衍生 非水 毛细管电泳 分离
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化妆品及保健品中Cr^(3+)和Cr^(6+)的柱后衍生-离子色谱法测定 被引量:8
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作者 钟志雄 朱炳辉 吴西梅 《分析测试学报》 CAS CSCD 北大核心 2008年第5期546-548,552,共4页
建立了化妆品和保健品中Cr3+、Cr6+的柱后衍生-离子色谱检测法。样品中的铬经柱前络合为2,6-嘧啶二羧酸铬,柱后衍生为1,5二苯碳酰二肼铬,于520 nm波长检测。优化了衍生反应条件,提高了检测灵敏度,Cr3+和Cr6+方法检出限(LODs,3σ)分别为0... 建立了化妆品和保健品中Cr3+、Cr6+的柱后衍生-离子色谱检测法。样品中的铬经柱前络合为2,6-嘧啶二羧酸铬,柱后衍生为1,5二苯碳酰二肼铬,于520 nm波长检测。优化了衍生反应条件,提高了检测灵敏度,Cr3+和Cr6+方法检出限(LODs,3σ)分别为0.12、0.011 mg/kg。样品加标回收率为89%~108%,重复性好。共存离子Fe2+、Fe3+、Pb2+、Cu2+、Al3+、Zn2+、Hg2+等,以及常见有机物均不干扰测定。 展开更多
关键词 柱后衍生 离子色谱法 化妆品 保健品 CR^3+ CR^6+
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HPLC在线二阶微分光谱法检测人表皮生长因子的C-端肽
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作者 张丽华 屠红 +2 位作者 吴高德 徐来根 夏其昌 《高技术通讯》 CAS CSCD 1995年第5期7-10,共4页
Trp(色氨酸)与Tyr(酪氨酸)二阶微分光谱能被用来在线检测RP-HPLC(反相高效液相色谱)中含芳香族氨基酸的肽段,这些特征光谱是通过二极管矩阵检测器(DAD)得到的,用这种方法分析人表皮生长因子(hEGF)的胰... Trp(色氨酸)与Tyr(酪氨酸)二阶微分光谱能被用来在线检测RP-HPLC(反相高效液相色谱)中含芳香族氨基酸的肽段,这些特征光谱是通过二极管矩阵检测器(DAD)得到的,用这种方法分析人表皮生长因子(hEGF)的胰蛋白酶酶解图谱,能鉴别出含Trp的C-端肽。 展开更多
关键词 二阶微分光谱 C-端肽序列分析 人表皮生长因子
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衍生化法测定水果中还原型谷胱甘肽(GSH)和总巯基含量 被引量:5
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作者 朴玉莲 沈丽 +1 位作者 韩玲 崔胜云 《食品工业科技》 CAS CSCD 北大核心 2012年第20期60-64,共5页
用衍生化试剂5,5-二硫硝基苯甲酸(DTNB)对巯基化合物的衍生化反应和邻苯二甲醛(OPA)与GSH的荧光衍生化反应,分别采用UV—vis分光光度法和荧光光度法测定了猕猴桃、大枣、酸浆中总巯基含量和GSH的含量。被测样品中GSH和总巯基相对... 用衍生化试剂5,5-二硫硝基苯甲酸(DTNB)对巯基化合物的衍生化反应和邻苯二甲醛(OPA)与GSH的荧光衍生化反应,分别采用UV—vis分光光度法和荧光光度法测定了猕猴桃、大枣、酸浆中总巯基含量和GSH的含量。被测样品中GSH和总巯基相对含量分布有较大的差异,其中猕猴桃和大枣中含有相对较高的GSH浓度分布,分别达0.7092、0.8374mol/g,酸浆中含有较高的总巯基含量分布,达1.4173mol/g。本测定方法的回收率达95.38%-101.9%,满足测定准确度的要求,总巯基含量和GSH含量测定最低检测限分别为9.6×10^-7,2.69×10^-7mol/L。 展开更多
关键词 谷胱甘肽 巯基化合物 衍生化 5 5-二硫双-2-硝基苯甲酸 邻苯二甲醛
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Evaluation of Baishao(Radix Paeoniae Alba)and Chishao(Radix Paeoniae Rubra)from different origins based on characteristic spectra of amino acids 被引量:2
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作者 LI Shasha ZHANG Yiping +4 位作者 DENG Yuanhui WANG Qian XIE Zhiru Ibarra-Estrada Emmanuel XIAO Xue 《Digital Chinese Medicine》 2021年第2期120-129,共10页
Objective To evaluate the difference between Baishao(Paeoniae Radix Alba,PRA)and Chishao(Paeoniae Radix Rubra,PRR)from different regions based on the characteristic spectra of amino acids(AAs).Methods Fingerprints of ... Objective To evaluate the difference between Baishao(Paeoniae Radix Alba,PRA)and Chishao(Paeoniae Radix Rubra,PRR)from different regions based on the characteristic spectra of amino acids(AAs).Methods Fingerprints of the 21 standard AAs were established using O-phthalaldehyde-9-fluorenylmethyl chloroformate(OPA-FMOC)pre-column derivation method.The AA components in PRA and PRR were characterized qualitatively and quantitatively,and different AAs were screened using pattern recognition technology.Results Twelve AAs were identified in both PRA and PRR.Meanwhile,aspartic acid,glutamic acid,arginine,alanine,and gamma-aminobutyric acid were screened as characteristic components,and their concentrations could effectively distinguish PRA from PRR.Conclusion The established characterization method,which is based on the characteristic spectra of AAs,is accurate,efficient,and sensitive and can effectively distinguish between PRA and PRR from different producing areas,thus providing a reference for the overall characterization and evaluation of Chinese medicinal materials. 展开更多
关键词 O-phthalaldehyde-9-fluorenylmethyl chloroformate(OPA-FMOC)pre-column derivatization method Amino acids characteristic spectra Baishao(Paeoniae Radix Alba PRA) Chishao(Paeoniae Radix Rubra PRR) Pattern recognition
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Contents Variation Analysis ofγ-Amino Butyric Acid in Semen sojae praeparatum Fermentation Using Online Pre-Column Derivatization-HPLC 被引量:2
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作者 WENG Meizhi HE Jing +5 位作者 REN Jiaxiu LIANG Yonghong CHEN Qingfeng SU Mingsheng WANG Liyuan XIE Xiaomei 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2021年第1期55-61,共7页
This paper reported the contents variation analysis ofγ-amino butyric acid(GABA)in Semen sojae praeparatum(SSP)which is a famous traditional Chinese medicine.High performance liquid chromatography(HPLC)was used and G... This paper reported the contents variation analysis ofγ-amino butyric acid(GABA)in Semen sojae praeparatum(SSP)which is a famous traditional Chinese medicine.High performance liquid chromatography(HPLC)was used and GABA was derivatized by online pre-column derivatization with o-phthalaldehyde(OPA).To validate this method,the precision,stability,repeatability and recovery were discussed.In the concentration range from 0.0125 to 0.400 mg/m L,the calibration curve for GABA was linear and the regression equation was obtained with correlation coefficient(R2)of 0.9999.Relatively high levels of GABA exist in SSP and the content changes of GABA at different time points during the fermenting process were detected.At the"yellow cladding"stage,GABA level was very low or even undetectable;the"secondary fermentation"stage witnessed a rapid increase of GABA content to 1.39-5.52 mg/g,which remained stable after 18 days of"secondary fermentation".This study demonstrated that GABA was generated at the"secondary fermentation"stage,revealing the significance and rationality of the"secondary fermentation"stage in the fermenting process of SSP.On the other hand,it suggested the downside of taking soy isoflavones as the only measurement in existing quality assessment and optimization approach for the fermenting process of SSP. 展开更多
关键词 Semen sojae praeparatum γ-amino butyric acid(GABA) "secondary fermentation"process online pre-column derivatization-high performance liquid chromatography
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Application of liquid pre-column capillary electrophoresis technique to the study of interaction between drug enantiomers and human serum albumin
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作者 丁永生 朱晓蜂 林炳承 《Science China Chemistry》 SCIE EI CAS 1999年第6期617-623,共7页
Based on the chiral separation of several basic drugs, dimetindene, tetryzoline, theodrenaline and verapamil, the liquid pre-column capillary electrophoresis (LPC-CE) technique was established. It was used to determin... Based on the chiral separation of several basic drugs, dimetindene, tetryzoline, theodrenaline and verapamil, the liquid pre-column capillary electrophoresis (LPC-CE) technique was established. It was used to determine free concentrations of dmg enantiomers in mixed solutions with human serum albumin (HSA). To prevent HSA entering the CE chiral srparation zone, the mobility differences between HSA and drugs under a specific pH condition were employed in thr LPC. Thus, the detection confusion caused by protein was totally avoided. Further study of binding constants determination and protein binding competitions was carried out. The study proves that the LPC technique could be used for complex mrdia, particularly the matrix of protein coexisting with a variety of dmgs. 展开更多
关键词 CAPILLARY ELECTROPHORESIS LIQUID pre-column BINDING constant chiral drug human serum albumin.
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含腙类结构化合物生物活性研究进展 被引量:6
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作者 康圣鸿 胡德禹 +1 位作者 吴剑 宋宝安 《农药》 CAS 北大核心 2012年第4期238-242,245,共6页
含腙类结构的化合物具有杀虫、杀菌、除草等广泛生物活性,在新农药创制中占有重要地位。根据农药用途进行分类,对其生物活性进行了介绍,并对其发展前景作进一步展望。
关键词 腙类衍生物 杀虫活性 杀菌活性 除草活性 进展
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含腙类化合物的设计、合成与生物活性
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作者 吴峤 王秀丽 +1 位作者 杨吉春 宋玉泉 《农药》 CAS CSCD 北大核心 2014年第9期631-632,646,共3页
[目的]含腙类结构的化合物具有杀虫、杀菌、除草等生物活性,在新农药创制中占有重要地位。发现具有更好生物活性的新化合物。[方法]利用新颖中间体合成含腙类化合物5个。并利用1H NMR等分析手段确定化合物的结构。[结果]生测活性测定表... [目的]含腙类结构的化合物具有杀虫、杀菌、除草等生物活性,在新农药创制中占有重要地位。发现具有更好生物活性的新化合物。[方法]利用新颖中间体合成含腙类化合物5个。并利用1H NMR等分析手段确定化合物的结构。[结果]生测活性测定表明某些化合物在600 mg/L下对小菜蛾显示出较好防效。该类化合物可作为先导化合物进行进一步优化研究。 展开更多
关键词 含腙类化合物 合成 生物活性
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Reversed-phase high-performance liquid chromatographic analysis of seven pairs of chiral drug enantiomers in transport medium after chiral derivatization
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作者 贺瑛 柴晓鹃 曾苏 《Journal of Chinese Pharmaceutical Sciences》 CAS 2010年第2期104-109,共6页
A reversed-phase high-performance liquid chromatographic (RP-HPLC) method was established for the determination of the enantiomers of 7 aryloxy aminopropanol drugs (atenolol, sotalol, celiprolol, carvedilol, metopr... A reversed-phase high-performance liquid chromatographic (RP-HPLC) method was established for the determination of the enantiomers of 7 aryloxy aminopropanol drugs (atenolol, sotalol, celiprolol, carvedilol, metoprolol, propranolol and propafenone) in transport medium. The method involved liquid-liquid extraction of chiral drugs from transport medium, and employed 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate (GITC, 1.0 mg/mL in acetonitrile) as a pre-column chiral derivatization reagent. After derivatization, the products were separated on an Agilent Zorbax C8 column (150 min×4.6 mm, 5 μm) at 25 ℃. The mobile phase consisted of a mixture of acetonitrile and 0.01 M phosphate buffer (pH 3.5). The present methods were specific for the determination of enantiomers of each chiral drug. The absolute recoveries of the enantiomers and internal standards were 〉78%. The relative recoveries of the enantiomers were approximately 100%. The intra- and inter-day variations were 〈 15%. The method was reproducible and sufficiently sensitive to determine the enantiomers of seven aryloxy aminopropanol drugs in transport medium. The method could be used to study the transport of atenolol, sotalol, celiprolol, carvedilol, metoprolol, propranolol and propafenone. 展开更多
关键词 Enantiomer separation Aryloxy aminopropanol drugs RP-HPLC pre-column derivatization
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