Stress tensor determination by modified hydraulic tests on pre-existing fractures:Method and stress constraints

The hydraulic testing of pre-existing fractures(HTPF)is one of the most promising in situ stress measurement methods,particularly for three-dimensional stress tensor determination.However,the stress tensor determination based on the HTPF method requires at least six tests or a minimum of 14-15 tests(under different conditions)for reliable results.In this study,we modified the HTPF method by considering the shear stress on each pre-existing fracture,which increased the number of equations for the stress tensor determination and decreased the number of tests required.Different shear stresses were attributed to different fractures by random sampling;therefore,the stress tensors were obtained by searching for the optimal solution using the least squares criterion based on the Monte Carlo method.Thereafter,we constrained the stress tensor based on the tensile strength criterion,compressive strength criterion,and vertical stress constraints.The inverted stress tensors were presented and analyzed based on the tensorial nature of the stress using the Euclidean mean stress tensor.Two stress-measurement campaigns in Weifang(Shandong Province,China)and Mercantour road tunnel(France)were implemented to highlight the validity and efficiency of the modified HTPF(M-HTPF)method.The results showed that the M-HTPF method can be applied for stress tensor inversion using only three to four tests on pre-existing fractures,neglecting the stress gradient.The inversion results were confined to relatively small distribution dispersions and were significantly reliable and stable due to the shear stresses on the fractures and the stress constraints employed.The M-HTPF method is highly feasible and efficient for complete stress tensor determination in a single borehole.

Human leukocyte antigen and donor-specific antibodies in liver transplantation

In this article,we comment on an article published in a recent issue of the World Journal of Gastroenterology.We specifically focus on the roles of human leukocyte antigen(HLA)and donor-specific antibodies(DSAs)in pediatric liver transpl-antation(LT),as well as the relationship between immune rejection after LT and DSA.Currently,LT remains the standard of care for pediatric patients with end-stage liver disease or severe acute liver failure.However,acute and chronic re-jection continues to be a significant cause of graft dysfunction and loss.HLA mismatch significantly reduces graft survival and increases the risk of acute rejection.Among them,D→R one-way mismatch at three loci was significantly related to graft-versus-host disease incidence after LT.The adverse impact of HLA-DSAs on LT recipients is already established.Therefore,the evaluation of HLA and DSA is crucial in pediatric LT.

New glucocerebrosidase antibodies can advance research in the field of neurodegenerative disorders

In medical research,there are times when the introduction of a new tool can launch scientific discovery in new directions.While antibody development may be considered mundane,in the field of glucocerebrosidase(GCase)research,the dearth of validated antibodies for different applications has impeded progress in studies of disease pathogenesis and therapeutic development.The recent introduction of new,rigorously evaluated antibodies can now propel research into the link between glucocerebrosidase and Parkinson’s disease(PD)as well as aspects of the pathobiology of Gaucher disease(Jong et al.,2024).

Expanding role of antibodies in kidney transplantation

The role of antibodies in kidney transplant(KT)has evolved significantly over the past few decades.This role of antibodies in KT is multifaceted,encompassing both the challenges they pose in terms of antibody-mediated rejection(AMR)and the opportunities for improving transplant outcomes through better detection,prevention,and treatment strategies.As our understanding of the immunological mechanisms continues to evolve,so too will the approaches to managing and harnessing the power of antibodies in KT,ultimately leading to improved patient and graft survival.This narrative review explores the multifaceted roles of antibodies in KT,including their involvement in rejection mechanisms,advancements in desensitization protocols,AMR treatments,and their potential role in monitoring and improving graft survival.

Serological surveillance for SARS-CoV-2 antibodies among students,faculty and staff within a large university system during the pandemic

BACKGROUND At the end of December 2019,the world faced severe acute respiratory syndrome-coronavirus 2(SARS-CoV-2),which led to the outbreak of coronavirus disease 2019(COVID-19),associated with respiratory issues.This virus has shown significant challenges,especially for senior citizens,patients with other underlying illnesses,or those with a sedentary lifestyle.Serological tests conducted early on have helped identify how the virus is transmitted and how to curb its spread.The study hypothesis was that the rapid serological test for SARS-CoV-2 antibodies could indicate the immunoreactive profile during the COVID-19 pandemic in a university population.AIM To conduct active surveillance for serological expression of anti-SARS-CoV-2 antibodies in individuals within a university setting during the COVID-19 pandemic.METHODS This sectional study by convenience sampling was conducted in a large university in Niteroi-RJ,Brazil,from March 2021 to July 2021.The study population consisted of students,faculty,and administrative staff employed by the university.A total of 3433 faculty members,60703 students,and 3812 administrative staff were invited to participate.Data were gathered through rapid serological tests to detect immunoglobulin(Ig)M and IgG against SARS-CoV-2.Theχ²or Fisher's exact test was used to conduct statistical analysis.A 0.20 significance level was adopted for variable selection in a multiple logistic regression model to evaluate associations.RESULTS A total of 1648 individuals were enrolled in the study.The proportion of COVID-19 positivity was 164/1648(9.8%).The adjusted logistic model indicate a positive association between the expression of IgM or IgG and age[odds ratio(OR)=1.16,95%CI:1.02-1.31](P<0.0024),individuals who had been in contact with a COVID-19-positive case(OR=3.49,95%CI:2.34-5.37)(P<0.001),those who had received the COVID-19 vaccine(OR=2.33,95%CI:1.61-3.35)(P<0.001)and social isolation(OR=0.59,95%CI:0.41-0.84)(P<0.004).The likelihood of showing a positive result increased by 16%with every ten-year increment.Conversely,adherence to social distancing measures decreased the likelihood by 41%.CONCLUSION These findings evidenced that the population became more exposed to the virus as individuals discontinued social distancing practices,thereby increasing the risk of infection for themselves.

Influences of pre-existing fracture on ground deformation induced by normal faulting in mixed ground conditions

Physical model tests have been conducted by various researchers to investigate fault rupture propagation and ground deformation induced by bedrock faulting. However, the effects of pre-existing fracture on ground deformation are not fully understood. In this work, six centrifuge tests are reported to investigate the influence of pre-existing fracture on ground deformation induced by normal faulting in sand, clay and nine-layered soil with interbedded sand and clay layers. Shear box tests were conducted to develop a filter paper technique, which was adopted in soil model preparation to simulate the effects of pre-existing fracture in centrifuge tests. Centrifuge test results show that ground deformation mechanism in clay, sand and nine-layered soil strata is classified as a stationary zone, a shearing zone and a rigid body zone. Inclination of the strain localization is governed by the dilatancy of soil material. Moreover, the pre-existing fracture provides a preferential path for ground deformation and results in a scarp at the ground surface in sand. On the contrary, fault ruptures are observed at the ground surface in clay and nine-layered soil strata.

A method to model the effect of pre-existing cracks on P-wave velocity in rocks

Crack closure is one of the reasons inducing changes of P-wave velocity of rocks under compression.In this context,a method is proposed to investigate the relationships among P-wave velocity,pre-existing cracks,and confining pressure based on the discrete element method(DEM).Pre-existing open cracks inside the rocks are generated by the initial gap of the flat-joint model.The validity of the method is evaluated by comparing the P-wave velocity tested on a sandstone specimen with numerical result.As the crack size is determined by the diameter of particles,the effects of three factors,i.e.number,aspect ratio,and orientation of cracks on the P-wave velocity are discussed.The results show that P-wave velocity is controlled by the(i.e.number) of open micro-cracks,while the closure pressure is determined by the aspect ratio of crack.The reason accounting for the anisotropy of P-wave velocity is the difference in crack number in measurement paths.Both of the number and aspect ratio of cracks can affect the responses of P-wave velocity to the applied confining pressure.Under confining pressure,the number of open cracks inside rocks will dominate the lowest P-wave velocity,and the P-wave velocity of the rock containing narrower cracks is more sensitive to the confining pressure.In this sense,crack density is difficult to be back-calculated merely by P-wave velocity.The proposed method offers a means to analyze the effect of pre-existing cracks on P-wave velocity.

Damage and failure characteristics of rock similar materials with pre-existing cracks

In order to further study the damage and failure mechanism for rock similar materials,this study investigated the mechanical properties and failure characteristics,law of damage space development,and damage evolution characteristics for rock similar materials with pre-existing cracks of varying length under uniaxial compression load.The equipment used in this study is the self-developed YYW-Ⅱ strain controlled unconfined compression apparatus and the PCIE-8 acoustic emission monitoring system.Results show that,as the length of pre-existing crack increases:(1) the peak and residual strength reduces,and the peak axial strain and the strain during the initial compression phase increases;(2) the major failure mode is changed from shear failure to tensile failure along a vertical plane that passes the middle of the pre-existing crack;(3) The damage increases during the stable and accelerated development stage,and the effect of the pre-existing cracks is more during the accelerated development stage than the stable development stage.

Effects of pre-existing cracks and infillings on strength of natural rocks--Cases of sandstone, argillite and basalt

This study aims to examine the influence of pre-existing discontinuities on the strengths of four natural rocks of different origins.A series of unconfined compression tests was performed on specimens of two types of sandstones,argillite and basalt that contain open and filled cracks.It was found that the presence of cracks tends to decrease the overall strength for all studied rocks;however,the magnitude of strength reduction is related to the property of rock.The larger strength decrease was observed for the relatively harder argillite and basalt,compared to the softer sandstone.It was also found that the infill material could increase the strength of rock specimens,while the obtained strength depended on the characteristics of the fill material.

Monoclonal antibodies as therapeutic agents in oncology and antibody gene therapy

Antibodies as therapeutic agents are mostly used in oncology, as illustrated by their applications in lymphoma, breast cancer or colorectal cancer. This review provides a brief historical sketch of the development of monoclonal antibodies for cancer treatment and summarizes the most significant clinical data for the best-established reagents to date. It also discusses strategies to improve the anti-tumor efficacy of antibody therapy, including antibody gene therapy and exploitation of bone marrow derived primary mesenchymal stem cells as the antibody gene transporter.

Preparation of Polyclonal Antibodies Against Testis-specific Protease 50 and Characterization of Antibody Specificity

Testis-specific protease 50 （TSP50） is a testis-specific oncogene, which is abnormally activated in most tested patients with breast cancer. This property makes it an attractive molecular marker and a promising target for the diagnosis and therapy of breast cancer. In order to obtain the protective and specific polyclonal antibodies for further research, TSPS0 cDNA was amplified by RT-PCR from normal human testicular tissue, and inserted into eukaryotic expression vector PeDNA3.1. Rabbit anti-TSPS0 polyclonal antibodies were prepared by means of intramuscular injection of peDNA3.1-TSPS0 into the rabbits. Titem of the anti-sera were measured by ELISA and Western blotting with the E. coli cell lysate containing the induced GST-TSPS0 fusion protein as an antigen. In addition, we examined the expression of TSPS0 in both breast cancer cell line MCF-7 and breast cancer tissue by immunofluorescent and immunohistochemistry analysis.

Seismic stability analysis of slopes with pre-existing slip surfaces

In analyzing seismic stability of a slope with upper bound limit analysis method, the slip surface is often assumed as a log-spiral or plane slip surface. However, due to the presence of a weak layer and unfavorable geological structural surface or a bedrock interface with overlying soft strata, the preexisting slip surface of the slope may be irregular and composed of a series of planes rather than strictly logspiral or plane shape. A computational model is developed for analyzing the seismic stability of slopes with pre-existing slip surfaces. This model is based on the upper bound limit analysis method and can consider the effect of anchor bolts. The soil or rock is deemed to follow the Mohr-Coulomb yield criterion. The slope is divided into multiple block elements along the slip surface. According to the displacement compatibility and the associated flow rule, a kinematic velocity field of the slope can be obtained computationally. The proposed model allows not only calculation of the rate of external work owing to the combined effect of self-weight and seismic loading, but also that of the energy dissipation rate caused by the slip surface, interfaces of block elements and anchorage effect of the anchors. Considering a direct relationship between the rate of external work and the energy dissipation rate, the expressions of yield acceleration and permanent displacement of anchored slopes can be derived. Finally, the validity of this proposed model is illustrated by analysis on three typical slopes. The results showed that the proposed model is more easily formulated and does not need to solve complex equations or time consuming iterations compared with previous methods based on the conditions of force equilibrium.

Relationship of Pre-Existing Maternal/Caregiver Acute Respiratory Infection in the Pattern and Risk of Acute Respiratory Infection among Infants in Rivers State, Nigeria

History of upper respiratory tract infection in the mother or siblings was associated with higher risk of acute lower respiratory tract infection in cases. Most upper respiratory tract infections were caused by viral pathogens and likely to occur in many members of the family. The study aimed to determine the existence and pattern of relationship between risk of acute respiratory infection (ARI) among infants and exposure to pre-existing maternal/caregiver acute respiratory tract infection. The study was designed as a community-based Nested case-control study of 1100 infants randomly selected from 12 communities out of 6 Local Government Areas of the 3 senatorial districts of Rivers State. A multistage random sampling technique was used in selecting the subjects up to the community level. Descriptive method was used to represent the characteristics of the subjects and the differences in ARI between exposed and unexposed infants were tested in a bivariate logistics regression at 5% level of significance. Odds ratio (OR) was used to interpret the size effect measures of ARI on exposure to pre-existing maternal/caregiver ARI differences. A total of 275 Cases of ARI and 825 controls were included in the study. Among exposed infants (N = 104), ARI cases were found to be higher n = 80 (76.9%) than in control n = 24 (23.1%). Whereas, among unexposed infants N = 991, ARI cases were found to be lower n = 195 (19.7%) than in control n = 796 (80.3%). For the exposed infants, the odds for ARI were 13.5 times significantly higher compared to those of their unexposed counterparts (OR-Unadjusted = 13.52, (p < 0.0001, 95% CI = 0.047 - 0.121)). The findings will widen the horizon in the etiological consideration of ARI among infants vis-à-vis exposure potential to pre-existing maternal/caregiver ARI via nursing care. Therefore, community-based sensitization programme on barrier nursing care techniques and personal hygiene practices should be on focus.

Influence of intermediate principal stress on failure mechanism of hard rock with a pre-existing circular opening

Based on particle flow theory, the influences of the magnitude and direction of the intermediate principal stress on failure mechanism of hard rock with a pre-existing circular opening were studied by carrying out true triaxial tests on siltstone specimen. It is shown that peak strength of siltstone specimen increases firstly and subsequently decreases with the increase of the intermediate principal stress. And its turning point is related to the minimum principal stress and the direction of the intermediate principal stress. Failure characteristic(brittleness or ductility) of siltstone is determined by the minimum principal stress and the difference between the intermediate and minimum principal stress. The intermediate principal stress has a significant effect on the types and distributions of microcracks. The failure modes of the specimen are determined by the magnitude and direction of the intermediate principal stress, and related to weakening effect of the opening and inhibition effect of confining pressure in essence: when weakening effect of the opening is greater than inhibition effect of confining pressure, the failure surface is parallel to the x axis(such as σ2=σ3=0 MPa); conversely, the failure surface is parallel to the z axis(such as σ2=20 MPa, σ3=0 MPa).

Oblique extension of pre-existing structures and its control on oil accumulation in eastern Bohai Sea

Based on 3D seismic data, the evolution mechanism and characteristics of faults were investigated to reveal the structural origin and its control on differential hydrocarbon accumulation through comprehensive analyses, including structure style analysis, fault activity analysis, analogue modelling and comparison among the wells. The complex fault system with differently trending faults resulted from strike-slip and rifting in Paleogene was partly activated, developed successively and stretched obliquely by the near-NS extensional stress field in Neogene. In the area little affected by pre-existing faults, new faults nearly perpendicular to the extension direction developed. The structural development in the study area was not caused by transpressional strike slip. Under the oblique extension effect of pre-existing faults, if the angle between the strike of pre-existing fault and the extensional direction is different, the strike-slip and extensional stresses are different in ratio. The larger the angle between the two is, the stronger the extensional component, the poorer the sealing ability of the fault, and the stronger the oil and gas migration capacity will be. Conversely, the smaller the angle between the two is, the stronger the strike-slip component, the better the sealing ability of the fault, and the poorer the oil and gas migration capacity will be. The accumulation condition analysis results considering the fault trend are in good agreement with the oil and gas shows in wells drilled in this area.

On the Reactivation of the Pre-Existing Normal Fault

The reactivation of pre-existing faults is a common phenomenon in a basin. This paper discusses the relationship between the pre-existing faults and the newly formed Coulomb shear fractures regarding pore fluid pressures. Based on the Coulomb fracture criterion and Byerlee frictional sliding criterion, an equation relating pore pressure coefficient (&lambda;e), minimum dip angle (αe) of the reactive pre-existing fault and the intersection point depth (z) between the pre-existing fault and a newly formed Coulomb shear fault in an extensional basin, is established in this paper. This equation enhanced the understanding on the reactivation of pre-existing faults and can be used to calculate paleo-pore fluid pressures. The bigger the pore fluid pressure in a pre-existing fault is, the less the minimum dip angle for a reactive pre-existing fault will be. The minimum dip angle is less in shallow area than that in deep area. This will be of significance in petroleum exploration and development.

On the Orientation of Fractures with Transpressional and Transtensional Wrenches in Pre-Existing Faults

The orientation of fractures with transpressional and transtensional wrenches in pre-existing faults has not been quantitatively determined. Based on Coulomb failure criterion and Byerlee’s frictional sliding criterion, this paper has indicated quantitative geometric relationships between the pre-existing fault and the local induced principal stress axes caused by the rejuvenation of the pre-existing fault. For a hidden pre-existing fault with some cohesion, the angles between the local induced principal stress axes and the pre-existing fault quantitatively vary with the applied stress and the cohesion coefficient, the ratio of the thickness of the cover layer to the thickness of the whole wrench body, whether transpressional or transtensional wrenches occur. For a surface pre-existing fault with zero cohesion, the angles between the pre-existing fault and the local induced principal stress axes are related to the rock inner frictional angle regardless of both the applied stress and the cohesion coefficient where transpressional wrenches occur, and the local induced maximum principal stress axis is identical with the applied maximum principal stress axis where transtensional wrenches occur. Therefore, the geometric relationships between the pre-existing faults and their related fractures are defined, because the local induced principal stress axes determine the directions of the related fractures. The results can be applied to pre-existing weak fabrics. They can help to understand and analyze wrench structures in outcrops or subsurface areas. They are of significance in petroleum exploration.

PRODUCTION AND APPLICATION OF MONOCLONAL ANTIBODY TO POLYAMINE (PREPARATION AND CHARACTERISTICS OF MONOCLONAL ANTIBODIES AGAINST SPERMIDINE)

A monoclonal antibody was first prepared by fusion of mouse myeloma cells (SP2/0-Ag-14) with spleen cells isolated from male BALB/ c mice immunized with spermidine-bovine serum albumin conjugate (SPD- BSA). The hybridoma cell line producing antibody specific for spermidine was cultured in vitro and after i. p. into mice, the ascitic fluid gave suitably high dilution titres (1: 106) by enzyme immunoassay. This monoclonal antibody is of IgG1 class and the bimolecular compleex with molecular weight of 52KD and 27 KD. The monoclonal antibody was clearly specific to spermidine comparing with spermine or putriscine. Monclonal antibody may prove to be useful in the rapid diagnosis and evaluation of patients with cancer.

ISOLATION OF ENDOTOXIN-SPECIFIC ANTIBODIES BY SELECTION OF AN SINGLE CHAIN PHAGE ANTIBODY LIBRARY

Objective: To isolate murine anti endotoxin single chain phage antibody from a constructed library. Methods: Total RNA was firstly extracted from murine splenic cells and mRNA was reverse-transcribed into cDNA. Then the designed primers were used to amplify the variable region genes of the heavy and light chain (VH, VL) with polymerase chain reaction. The linker was used to assemble the VH and VL into ScFv, and the NotI and SfiI restriction enzymes were used to digest the ScFv in order to ligate into the pCANTAB5E phagemid vector that was already digested with the same restriction enzymes. The ligated vector was then introduced into competent E.coli TG1 cells to construct a single-chain phage antibody library. After rescued with M13KO7 helper phage, recombinant phages displaying ScFv fragments were harvested from the supernatant and selected with endotoxin. The enriched positive clones were reinfected into TG1 cells. Finally, 190 clones were randomly selected to detect the anti endotoxin antibody with indirect ELISA. Results: The titer of anti endotoxin in murine sera was 1:12,800. The concentration of total RNA was 12.38 μg/ml. 1.9×107 clones were obtained after transformed into TG1. 3×104 colonies were gotten after one round panning. Two positive colonies were confirmed with indirect ELISA among 190 randomly selected colonies. Conclusion: A 1.9×107 murine anti endotoxin single chain phage antibody library was successfully constructed. Two anti endotoxin antibodies were obtained from the library.

Blockade of Rho-associated kinase prevents inhibition of axon regeneration of peripheral nerves induced by anti-ganglioside antibodies

Anti-ganglioside antibodies are associated with delayed/poor clinical recovery in Guillain-Barrèsyndrome,mostly related to halted axon regeneration.Cross-linking of cell surface gangliosides by anti-ganglioside antibodies triggers inhibition of nerve repair in in vitro and in vivo paradigms of axon regeneration.These effects involve the activation of the small GTPase Rho A/ROCK signaling pathways,which negatively modulate growth cone cytoskeleton,similarly to well stablished inhibitors of axon regeneration described so far.The aim of this work was to perform a proof of concept study to demonstrate the effectiveness of Y-27632,a selective pharmacological inhibitor of ROCK,in a mouse model of axon regeneration of peripheral nerves,where the passive immunization with a monoclonal antibody targeting gangliosides GD1a and GT1b was previously reported to exert a potent inhibitory effect on regeneration of both myelinated and unmyelinated fibers.Our results demonstrate a differential sensitivity of myelinated and unmyelinated axons to the pro-regenerative effect of Y-27632.Treatment with a total dosage of 9 mg/kg of Y-27632 resulted in a complete prevention of anti-GD1a/GT1b monoclonal antibody-mediated inhibition of axon regeneration of unmyelinated fibers to skin and the functional recovery of mechanical cutaneous sensitivity.In contrast,the same dose showed toxic effects on the regeneration of myelinated fibers.Interestingly,scale down of the dosage of Y-27632 to 5 mg/kg resulted in a significant although not complete recovery of regenerated myelinated axons exposed to anti-GD1a/GT1b monoclonal antibody in the absence of toxicity in animals exposed to only Y-27632.Overall,these findings confirm the in vivo participation of Rho A/ROCK signaling pathways in the molecular mechanisms associated with the inhibition of axon regeneration induced by anti-GD1a/GT1b monoclonal antibody.Our findings open the possibility of therapeutic pharmacological intervention targeting Rho A/Rock pathway in immune neuropathies associated with the presence of anti-ganglioside antibodies and delayed or incomplete clinical recovery after injury in the peripheral nervous system.