Effects of thrombopoietin pre-treatment on peri-liver transplantation thrombocytopenia in a mouse model of cirrhosis with hypersplenism

BACKGROUND During cirrhosis,the liver is impaired and unable to synthesize and clear thrombopoietin properly.At the same time,the spleen assumes the function of hemofiltration and storage due to liver dysfunction,resulting in hypersplenism and excessive removal of platelets in the spleen,further reducing platelet count.When liver function is decompensated in cirrhotic patients,the decrease of thrombopoietin(TPO)synthesis is the main reason for the decrease of new platelet production.This change of TPO leads to thrombocytopenia and bleeding tendency in cirrhotic patients with hypersplenism.AIM To investigate the clinical efficacy of recombinant human TPO(rhTPO)in the treatment of perioperative thrombocytopenia during liver transplantation in cirrhotic mice with hypersplenism.METHODS C57BL/6J mice and TPO receptor-deficient mice were used to establish models of cirrhosis with hypersplenism.Subsequently,these mice underwent orthotopic liver transplantation(OLT).The mice in the experimental group were given rhTPO treatment for 3 consecutive days before surgery and 5 consecutive days after surgery,while the mice in the control group received the same dose of saline at the same frequency.Differences in liver function and platelet counts were determined between the experimental and control groups.Enzyme-linked immunosorbent assay was used to assess the expression of TPO and TPO receptor(c-Mpl)in the blood.RESULTS Preoperative administration of rhTPO significantly improved peri-OLT thrombocytopenia in mice with cirrhosis and hypersplenism.Blocking the expression of TPO receptors exacerbated peri-OLT thrombocytopenia.The concentration of TPO decreased while the concentration of c-Mpl increased in compensation in the mouse model of cirrhosis with hypersplenism.TPO pre-treatment significantly increased the postoperative TPO concentration in mice,which in turn led to a decrease in the c-Mpl concentration.TPO pre-treatment also significantly enhanced the Janus kinase(Jak)/signal transducers and activators of transcription pathway protein expressions in bone marrow stem cells of the C57BL/6J mice.Moreover,the administration of TPO,both before and after surgery,regulated the levels of biochemical indicators,such as alanine aminotransferase,alkaline phosphatase,and aspartate aminotransferase in the C57BL/6J mice.CONCLUSION Pre-treatment with TPO not only exhibited therapeutic effects on perioperative thrombocytopenia in the mice with cirrhosis and hypersplenism,who underwent liver transplantation but also significantly enhanced the perioperative liver function.

High corrosion resistance of electroless Ni-P with chromium-free conversion pre-treatments on AZ91D magnesium alloy

Phosphate-manganese, tannic acid and vanadium conversion coatings were proposed as an effective pre-treatment layer between electroless Ni-P coating and AZ91D magnesium alloy substrate to replace the traditional chromate plus HF pre-treatment. The electrochemical results show that the chrome-free coatings plus electroless Ni-P coating on the magnesium alloy has the lowest corrosion current density and most positive corrosion potential compared with chromate plus electroless Ni-P coating on the magnesium alloy. These proposed pre-treatment layers on the substrate reduce the corrosion of magnesium during plating process, and reduce the potential difference between the matrix and the second phase. Thus, an electroless Ni-P coating with fine crystalline and dense structure was obtained, with preferential phosphorus content, low porosity, good corrosion-resistance and strengthened adhesion than the chromate plus electroless Ni-P.

Comparison of pre-treatment and post-treatment use of selenium in retinal ischemia reperfusion injury

AIM: To investigate the effects of selenium in rat retinal ischemia reperfusion(IR) model and compare pretreatment and post-treatment use.METHODS: Selenium pre-treatment group(n =8) was treated with intraperitoneal(i.p.) selenium 0.5 mg/kg for7 d and terminated 24 h after the IR injury. Selenium posttreatment group( n = 8) was treated with i. p. selenium0.5 mg/kg for 7d after the IR injury with termination at the end of the 7d period. Sham group(n =8) received i.p.saline injections identical to the selenium volume for 7d with termination 24 h after the IR injury. Control group(n =8) received no intervention. Main outcome measures were retina superoxide dismutase(SOD), glutathione(GSH),total antioxidant status(TAS), malondialdehyde(MDA),DNA fragmentation levels, and immunohistological apoptosis evaluation.RESULTS: Compared to the Sham group, selenium pre-treatment had a statistical difference in all parameters except SOD. Post-treatment selenium also resulted in statistical differences in all parameters except the MDA levels. When comparing selenium groups, the pre-treatment selenium group had a statistically higher success in reduction of markers of cell damage such as MDA and DNA fragmentation. In contrast, the post-selenium treatment group had resulted in statisticallyhigher levels of GSH. Histologically both selenium groups succeeded to limit retinal thickening and apoptosis. Pre-treatment use was statistically more successful in decreasing apoptosis in ganglion cell layer compared to post-treatment use.CONCLUSION: Selenium was successful in retinal protection in IR injuries. Pre-treatment efficacy was superior in terms of prevention of tissue damage and apoptosis.

Effect of Cross-flow Velocity on the Critical Flux of Ceramic Membrane Filtration as a Pre-treatment for Seawater Desalination

Pre-treatment, which supplies a stable, high-quality feed for reverse osmosis （RO） membranes, is a criti- cal step for successful operation in a seawater reverse osmosis plant. In this study, ceramic membrane systems were employed as pre-treatment for seawater desalination. A laboratory experiment was performed to investigate the effect of the cross-flow velocity on the critical flux and consequently to optimize the permeate flux. Then a pilot test was performed to investigate the long-term performance. The result shows that there is no significant effect of the cross-flow velocity on the critical flux when the cross-flow velocity varies in laminar flow region only or in turbulent flow region only, but the effect is distinct when the cross-flow velocity varies in the transition region. The membrane fouling is slight at the permeate flux of 150 L·m^-2·h^-1 and the system is stable, producing a high-quality feed （the turbidity and silt density index are less than 0.1 NTU and 3.0, respectively） for RO to run for 2922.4 h without chemical cleaning. Thus the ceramic membranes are suitable to filtrate seawater as the pre-treatment for RO.

Effect of microwave pre-treatment on physical quality,bioactive compounds,safety risk factor,and storage stability of peanut butter

Microwave,as a new heat treatment technology,has the characteristics of uniform and fast heating speed.It is an energy-saving technology known for improving oilseed product quality.Its efficiency mainly depends on the roasting power and time.However,the production of high-quality peanut butter using short-time roasting con-ditions are limited.Herein,we determined an appropriate microwave roasting power and time for peanuts and evaluated its impacts on the quality of peanut butter.Different roasting powers(400 W,800 W and 1200 W)and times(4,4.5,5,and 5.5 min)were preliminarily tested.Among them,800 W at 5 min was the most suitable.The roasting efficiency was further evaluated using color,sensory,bioactive compounds,storage stability,and safety risk factors of peanut butter produced from four peanut cultivars(Silihong,Baisha-1016,Yuanza-9102,and Yuhua-9414).The pre-treated butter obtained from three cultivars(Silihong,Yuanza-9102,and Yuhua-9414)with moisture content between 5%and 7.2%had a similar sensory score(6-7)as the commercial on a 9-point hedonic scale compare to the other.The color of the pre-treated peanut butter varies statistically with the commercial but remained in the recommended range of Hunter L*values of 51-52,respectively,for high initial moisture peanut cultivars.The total polyphenol(35.20-31.59±0.59μmol GAE/g)and tocopherol(19.05±0.35 mg/100 g)content in the butter obtained from three cultivars(Yuahua-9102,Yuhua,and Baisha-1016)and Silihong respectively,were significantly(P<0.05)higher than those in the commercial butter.The induction times of all pre-treated butter(19.80±0.99-7.84±0.07 h)were significantly(P<0.05)longer during storage at accelerated temperature than commercial samples.In addition,no benzo[a]pyrene was found in the pre-treated samples.Collectively,the microwave pretreated peanut butter was superior to the commercial one.These findings provided data support and a reference basis to promote microwave use for peanut butter production.

Artificial neural network approach for rheological characteristics of coal-water slurry using microwave pre-treatment

Detailed experimental investigations were carried out for microwave pre-treatment of high ash Indian coal at high power level(900 W) in microwave oven. The microwave exposure times were fixed at60 s and 120 s. A rheology characteristic for microwave pre-treatment of coal-water slurry(CWS) was performed in an online Bohlin viscometer. The non-Newtonian character of the slurry follows the rheological model of Ostwald de Waele. The values of n and k vary from 0.31 to 0.64 and 0.19 to 0.81 Pa·sn,respectively. This paper presents an artificial neural network(ANN) model to predict the effects of operational parameters on apparent viscosity of CWS. A 4-2-1 topology with Levenberg-Marquardt training algorithm(trainlm) was selected as the controlled ANN. Mean squared error(MSE) of 0.002 and coefficient of multiple determinations(R^2) of 0.99 were obtained for the outperforming model. The promising values of correlation coefficient further confirm the robustness and satisfactory performance of the proposed ANN model.

Tree-ring δ^(15)N of Qinghai spruce in the central Qilian Mountains of China:Is pre-treatment of wood samples necessary?

A knowledge of the tree-ring stable nitrogen isotope ratio(δ^(15)N)can deepen our understanding of forest ecosystem dynamics by indicating the long-term availability,cycling and sources of nitrogen(N).However,the radial mobility of N blurs the interannual variations in the long-term N records.Previous studies of the chemical extraction of tree rings before analysis had produced inconsistent results and it is still unclear whether it is necessary to pre-treat wood samples from specific tree species to remove soluble N compounds before determining theδ^(15)N values.We compared the effects of pre-treatment with organic solvents and hot ultrapure water on the N concentration andδ^(15)N of tree rings from endemic Qinghai spruce(Picea crassifolia)growing in the interior of the central Qilian Mountains,China,during the last 60 a.We assessed the effects of different preparation protocols on the removal of the labile N compounds and investigated the need to pre-treat wood samples before determining theδ^(15)N values of tree rings.Increasing trends of the tree-ring N concentration were consistently observed in both the extracted and unextracted wood samples.The total N removed by extraction with organic solvents was about 17.60%,with a significantly higher amount in the sapwood section(P<0.01).Theδ^(15)N values of tree rings decreased consistently from 1960 to 2019 in both the extracted and unextracted wood samples.Extraction with organic solvents increased theδ^(15)N values markedly by about 5.2‰and reduced the variations in theδ^(15)N series.However,extraction with hot ultrapure water had little effect,with only a slight decrease in theδ^(15)N values of about 0.5‰.Our results showed that the radial pattern in the inter-ring movement of N in Qinghai spruce was not minimized by extraction with either organic solvents or hot ultrapure water.It is unnecessary to conduct hot ultrapure water extraction for the wood samples from Qinghai spruce because of its negligible effect on the removal of the labile N.Theδ^(15)N variation trend of tree rings in the unextracted wood samples was not influenced by the heartwood-sapwood transition zone.We suggest that theδ^(15)N values of the unextracted wood samples of the climate-sensitive Qinghai spruce could be used to explore the ecophysiological dynamics while focusing on the long-term variations.

Pre-treatment to Enhance Biogas Yield from Pulp and Paper Mill Sludge

The purpose of this work was to study the potential to enhance biogas production from pulp and paper mill sludge by the use of thermal pre-treatment in combination with chemical pre-treatment. Biogas from waste is a renewable fuel with very low emissions during combustion. To further reduce the use of fossil fuels, more biogas substrates are necessary. Pulp and paper mill sludge is a large untapped reservoir of potential biogas. Pulp and paper mill sludge was collected from a mill that produces both pulp and paper and has a modified waste activated sludge system as part of its wastewater treatment. Pre-treatments were chosen heat （70 ~C or 140℃） combined with either acid （pH 2 or pH 4） or base （pH 9 or pH 11, obtained with Ca（OH）2 or NaOH）. Biogas potential was tested by anaerobic digestion batch assays under mesophilic conditions. All pre-treatments were tested in six replicates. Biogas volume was measured with a gas-tight syringe and methane concentration was measured with a gas chromatograph. The methane yield from sludge subjected to thermal pre-treatment at 70℃ did not differ from the untreated sludge, but thermal pre-treatment at 140℃ had a positive effect. Within the 70℃ thermal pre-treatment group, the pH 2 acid was the most successful chemical pre-treatment, and Ca（OH）2 pH 9 had the least effect with no measurable improvement in methane yield. For the 140 ℃ thermal pre-treatment group, acid and NaOH impacted methane production negatively, while the Ca（OH）2-treated sludge did not differ from sludge with no chemical pre-treatment. In conclusion, thermal pre-treatment at 70℃ showed no effect, whereas, pre-treatment at 140℃ improved methane yield with 170%, and for this sludge additional, chemical pre-treatments are unnecessary.

Priming mesenchymal stem cells to develop “super stem cells”

The stem cell pre-treatment approaches at cellular and sub-cellular levels encompass physical manipulation of stem cells to growth factor treatment,genetic manipulation,and chemical and pharmacological treatment,each strategy having advantages and limitations.Most of these pre-treatment protocols are non-combinative.This editorial is a continuum of Li et al’s published article and Wan et al’s editorial focusing on the significance of pre-treatment strategies to enhance their stemness,immunoregulatory,and immunosuppressive properties.They have elaborated on the intricacies of the combinative pre-treatment protocol using pro-inflammatory cytokines and hypoxia.Applying a well-defined multi-pronged combinatorial strategy of mesenchymal stem cells(MSCs),pre-treatment based on the mechanistic understanding is expected to develop“Super MSCs”,which will create a transformative shift in MSC-based therapies in clinical settings,potentially revolutionizing the field.Once optimized,the standardized protocols may be used with slight modifications to pre-treat different stem cells to develop“super stem cells”with augmented stemness,functionality,and reparability for diverse clinical applications with better outcomes.

Enhancing lead-free photovoltaic performance:Minimizing buried surface voids in tin perovskite films through weakly polar solvent pre-treatment strategy

Buried interfacial voids have always been a notorious phenomenon observed in the fabrication of lead perovskite films. The existence of interfacial voids at the buried interface will capture the carrier, suppress carrier transport efficiencies, and affect the stability of photovoltaic devices. However, the impact of these buried interfacial voids on tin perovskites, a promising avenue for advancing lead-free photovoltaics, has been largely overlooked. Here, we utilize an innovative weakly polar solvent pretreatment strategy(WPSPS) to mitigate buried interfacial voids of tin perovskites. Our investigation reveals the presence of numerous voids in tin perovskites during annealing, attributed to trapped dimethyl sulfoxide(DMSO) used in film formation. The WPSPS method facilitates accelerated DMSO evaporation, effectively reducing residual DMSO. Interestingly, the WPSPS shifts the energy level of PEDOT:PSS downward, making it more aligned with the perovskite. This alignment enhances the efficiency of charge carrier transport. As the result, tin perovskite film quality is significantly improved,achieving a maximum power conversion efficiency approaching 12% with only an 8.3% efficiency loss after 1700 h of stability tests, which compares well with the state-of-the-art stability of tin-based perovskite solar cells.

Acidizing & Acid-Fracturing Technology

EATAC(Fracturing&AcidizingTechniqueServiceCenter),subordinatetoChinaNatinalPetroleumCorp.(CNPC),isaspecializedresearchcenterinreservoristimulatonfnationaoilandgasfieds.FATSCismailyengagedinstudyingofappliedtechnique,advancedlabtechniqueandseriesofbas...

Potential role of Lycium barbarum polysaccharides in glaucoma management:evidence from preclinical in vivo studies

In recent years,the pharmacological benefits of herbal extracts have been revisited for their potential neuroprotective effects in glaucoma.The polysaccharides extracted from the fruits of Lycium barbarum L.,or Lycium barbarum polysaccharides,exert their anti-aging effect through reducing oxidative stress,modulating the immune response,enhancing neuronal responses,and promoting cytoprotection.The therapeutic efficacy of Lycium barbarum polysaccharides in preserving retinal ganglion cells and their functions was demonstrated in a range of experimental models of optic neuropathies.These include the acute and chronic ocular hypertension models,the partial optic nerve transection model,and the ischemic-reperfusion injuries model.Based on these findings,Lycium barbarum polysaccharides appear to be a good candidate to be developed as a neuroprotective agent for treating multifactorial diseases.This review aims to present a comprehensive review on the latest preclinical evidence on the pre-and post-treatment benefits of Lycium barbarum polysaccharides in retinal ganglion cell neuroprotection.The possible mechanisms of Lycium barbarum polysaccharides mediating retinal ganglion cell neuroprotection will also be described.Moreover,the potential research gaps in the effective translation of Lycium barbarum polysaccharides treatment into clinical glaucoma management will be discussed.

Design and Research of High-throughput Automation System for Food Testing Laboratory

In the process of food testing,human operation is an important variable affecting the experimental results.In order to reasonably avoid the influence of human subjective operation behavior on the accuracy of detection results,the laboratory information management system was used as the information platform to design a high-throughput laboratory automation pre-treatment system based on the deep integration of mechanical principles,visual analysis,high-speed conduction,intelligent storage and other technical systems.The experimental results showed that the system could shorten the sample circulation cycle,effectively improve the laboratory biosafety,and meet the requirements of high-throughput processing of samples.

Effect of Pre-Sowing Treatments on Mamalis (Pittosporum pentandrum Blanco Merr.) Seeds Germination under Nursery Condition

Seeds were subjected to three different pre-sowing seed treatments: immersion in lukewarm water for 2 hours, immersion in concentrated sulfuric acid (H2SO4) for 5 minutes, hilar removal, and a control in which the seeds were sown without being treated. The experiment was laid out in the Completely Randomized Design (CRD) with four replicates and 60 seeds per treatment. Seeds were sown in an improvised Seedbox in October 2019. Germination was monitored daily for one month. The results showed that Mamalis seeds treated with lukewarm water have the earliest germination of twelve days, with a germination percentage of 66.67%. The germination rate of another treatment ranges from 0 - 44 percent, compared to 45 percent for the control treatment. It seems prudent to conclude that to enhance the vegetative propagation methods is to soaking in warm water at 37.5˚C for 2 hours could provide the best growth.

Silver Nanowire Electrodes: Conductivity Improvement Without Post-treatment and Application in Capacitive Pressure Sensors

Transparent electrode based on silver nanowires(Ag NWs) emerges as an outstanding alternative of indium tin oxide film especially for flexible electronics. However, the conductivity of Ag NWs transparent electrode is still dramatically limited by the contact resistance between nanowires at high transmittance. Polyvinylpyrrolidone(PVP) layer adsorbed on the nanowire surface acts as an electrically insulating barrier at wire–wire junctions, and some devastating post-treatment methods are proposed to reduce or eliminate PVP layer, which usually limit the application of the substrates susceptible to heat or pressure and burden the fabrication with high-cost, time-consuming, or inefficient processes. In this work, a simple and rapid pre-treatment washing method was proposed to reduce the thickness of PVP layer from 13.19 to0.96 nm and improve the contact between wires. Ag NW electrodes with sheet resistances of 15.6 and 204 X sq-1have been achieved at transmittances of 90 and 97.5 %, respectively. This method avoided any post-treatments and popularized the application of high-performance Ag NW transparent electrode on more substrates. The improved Ag NWs were successfully employed in a capacitive pressure sensor with high transparency, sensitivity, and reproducibility.

Pretreatment of coking wastewater using anaerobic sequencing batch reactor (ASBR)

A laboratory-scale anaerobic sequencing batch reactor (ASBR) was used to pretreat coking wastewater. Inoculated anaerobic granular biomass was acclimated for 225 d to the coking wastewater, and then the biochemical methane potential (BMP)of the coking wastewater in the acclimated granular biomass was measured. At the same time, some fundamental technological factors, such as the filling time and the reacting time ratio (tf/tr), the mixing intensity and the intermittent mixing mode, that affect anaerobic pretreatment of coking wastewater with ASBR, were evaluated through orthogonal tests. The COD removal efficiency reached 38％～50％ in the stable operation period with the organic loading rate of 0.37～0.54 kg COD/(m3.d) at the optimum conditions of tf/tr, the mixing intensity and the intermittent mixing mode. In addition, the biodegradability of coking wastewater distinctly increased after the pretreatment using ASBR. At the end of the experiment, the microorganism forms on the granulated sludge in the ASBR were observed using SEM (scanning electron microscope) and fluoroscope. The results showed that the dominant microorganism on the granular sludge was Methanosaeta instead of Methanosarcina dominated on the inoculated sludge.

Impact of ozone assisted ultrasonication pre-treatment on anaerobic digestibility of sewage sludge

Impact of ultrasonication（ULS） and ultrasonication-ozonation（ULS-Ozone） pre-treatment on the anaerobic digestibility of sewage sludge was investigated with semi-continuous anaerobic reactors at solid retention time（SRT） of 10 and 20 days. The control, ULS and ULS-Ozone reactors produced 256, 309 and 348 m L biogas/g CODfedand the volatile solid（VS） removals were 35.6%, 38.3% and 42.1%, respectively at SRT of 10 days. At SRT of20 days, the biogas yields reached 313, 337 and 393 m L biogas/g CODfedand the VS removal rates were 37.3%, 40.9% and 45.3% in the control, ULS and ULS-Ozone reactors, respectively.ULS-Ozone pre-treatment increased the residual organic amount in the digested sludge.These soluble residual organics were found to contain macromolecules with molecular weights（MW） larger than 500 k Da and smaller polymeric products with MW around 19.4and 7.7 k Da. These compounds were further characterized to be humic acid-like substances with fluorescent spectroscopy analysis.

Optimized pre-treatment of high strength food waste digestate by high content aluminum-nanocluster based magnetic coagulation

Coagulation-based pre-treatment efficiency of high strength digestate of food waste(HSDFW) anaerobic digestion is negated by organic ligand-catalyzed decomposition of coagulants. In this study, an efficient HSDFW pre-treatment method, magnetic seeds(MS) coagulation, was employed by using highly stable Keggin Al_(30) nanocluster(PAC_(30)), MS and polyacrylamide(PAM), and its operation was optimized by evaluating the performance of removing turbidity, total suspended solids(TSS), chemical oxygen demand(COD), and total phosphorous(TP) phosphate. Results showed that at the optimum dosage of 4.82 g/L, PAC_(30) demonstrated excellent removals as high as 98.93% ± 0.1% of turbidity, 98.04% ± 0.1% of TSS, 58.28% ± 0.3% of total COD, 99.98% ± 0.01% of TP and 99.50% ± 0.01% of dissolved phosphate, respectively. Apparent molecular weight(AMW) and three-dimensional excitation-emission matrix(3 D-EEM) fluorescence spectroscopy analyses demonstrated more efficient removal of dissolved organic matter(DOM), particularly non-biodegradable and hydrophobic components by PAC_(30) than commercial coagulant. The sedimentation was much improved from 40 min by coagulation/flocculation to about 5 min settling by MS coagulation. The PAC_(30) based magnetic coagulation(MC) presents theoretical guidance on a cost-effective and much less footprint pre-treatment alternative for high strength wastewater.

Reduction of ischemia reperfusion injury after liver resection and hepatic inflow occlusion by α-lipoic acid in humans

AIM： To evaluate the protective effects of preconditioning by α-lipoic acid （LA） in patients undergoing hepatic resection under inflow occlusion of the liver. METHODS： Twenty-four patients undergoing liver resection for various reasons either received 600 mg LA or NaCI 15 min before transection performed under inflow occlusion of the liver. Blood samples and liver wedge biopsy samples were obtained after opening of the abdomen immediately after inflow occlusion of the liver, and 30 min after the end of inflow occlusion of the liver. RESULTS： Serum levels of aspartate transferase and alanine transferase were reduced at all time points in patients who received LA in comparison to those who received NaCL. This was accompanied by reduced histomorphological features of oncosis. We observed TUNEL-positive hepatocytes in the livers of the untreated patients, especially after 30 min of ischemia. LA attenuated this increase of TUNEL-positive hepatocytes. Under preconditioning with LA, ATP content was significantly enhanced after 30 min of ischemia and after 30 min of reperfusion. CONCLUSION： This is the first report on the potential for LA reducing ischemia/reperfusion injury （IRI） of the liver in humans who were undergoing liver surgery. Beside its simple and rapid application, side effects did not occur. LA might therefore represent a new strategy against hepatic IRI in humans.