Metastin expression in the placenta tissue of preeclampsia and its effect on the trophoblast cell proliferation and apoptosis

Objective: To investigate the metastin expression in the placenta tissue of preeclampsia and its effect on the trophoblast cell proliferation and apoptosis. Methods: A total of 80 puerperae with preeclampsia who gave birth in this hospital between September 2014 and December 2016 were collected as observation group and 50 normal puerperae who gave birth in the hospital during the same period were collected as normal control group. The metastin protein expression as well as the trophoblast cell proliferation and apoptosis gene expression in placenta tissue were compared between the two groups of puerperae, and Pearson test was used to evaluate the metastin expression in the placenta tissue of preeclampsia and its effect on the trophoblast cell proliferation and apoptosis. Results: Metastin protein expression in the placenta tissue of observation group was significantly higher than that of normal control group. Trophoblast cell proliferation genes Notch1 and HLA-G mRNA expression in the placenta tissue of observation group were lower than those of normal control group whereas PHLDA2 mRNA expression was higher than that of normal control group;apoptosis genes bcl-2 mRNA expression was lower than that of control group whereas bax, Fas and caspase-3 mRNA expression were higher than those of normal control group. Pearson test showed that metastin protein expression in the placenta tissue of puerperae with preeclampsia was directly correlated with the trophoblast cell proliferation and apoptosis gene expression. Conclusion: There is abnormally high metastin protein expression in placenta tissue of puerperae with preeclampsia, and it can directly affect the proliferation and apoptosis process of trophoblast cells.

TGF-β和IGF表达异常与滋养层相关疾病关系的研究

目的:探讨转化生长因子(TGF-β)和胰岛素样生长因子(IGF)等与滋养层侵入调节密切相关的细胞因子在胎盘绒毛组织中的表达,及与葡萄胎、先兆子痫等滋养层细胞相关妊娠疾病病理机制的关系。方法:采用半定量逆转录多聚酶链反应(RT-PCR)检测方法,分别以正常早孕绒毛和正常足月(自然分娩或剖宫产)胎盘绒毛组织为对照,观察葡萄胎组织与先兆子痫足月胎盘绒毛组织中TGF-β和IGF的表达。结果:5种胎盘绒毛组织中均可检测到IGF-I mRNA的表达,且先兆子痫足月胎盘绒毛组织中IGF-I的表达量明显低于正常足月胎盘绒毛组织,而葡萄胎组织中IGF-I的表达略高于正常早孕绒毛。在3种足月胎盘绒毛组织中可检测到IGF-ⅡmRNA的表达,但相互之间无明显差异。葡萄胎组织中IGF-Ⅱ的表达低于早孕绒毛。5种胎盘绒毛组织中均有TGF-β3的表达,而TGF-β1和TGF-β2的表达并未检测到。在表达量上,葡萄胎组织中TGF-β3的表达明显高于正常早孕绒毛组织,先兆子痫胎盘绒毛组织中TGF-β3的表达强于足月自然分娩的正常胎盘绒毛组织,而剖宫产胎盘绒毛组织中,TGF-β3的表达亦相对较强。结论:TGF-β3和IGF-I在胎盘绒毛组织中的表达变化,可能和葡萄胎、先兆子痫等与滋养层密切相关的妊娠疾病的发生有关。

子痫前期产妇胎盘组织中metastin表达量及其对滋养细胞增殖、凋亡的影响

目的:探讨子痫前期产妇胎盘组织中metastin表达量及其对滋养细胞增殖、凋亡的影响。方法:收集2014年9月~2016年12月间在本院分娩的子痫前期产妇80例作为观察组,同期在本院进行分娩的正常产妇50例作为正常对照组。对比两组产妇胎盘组织中metastin蛋白的表达量,滋养细胞增殖、凋亡基因的表达量,采用Pearson检验评估子痫前期产妇胎盘组织中metastin表达量及其对滋养细胞增殖、凋亡的影响。结果:观察组产妇胎盘组织中metastin蛋白的表达量显著高于正常对照组产妇(P<0.05)。观察组产妇胎盘组织中滋养细胞增殖基因Notch1、HLA-G mRNA的表达量低于正常对照组,PHLDA2mRNA的表达量高于正常对照组产妇;凋亡基因bcl-2mRNA的表达量低于正常对照组,bax、Fas、caspase-3mRNA的表达量高于正常对照组产妇(P<0.05)。经Pearson检验发现,子痫前期产妇胎盘组织中metastin蛋白表达量与滋养细胞增殖、凋亡基因表达量均直接相关。结论:子痫前期产妇胎盘组织中存在metastin蛋白异常高表达,且可直接影响滋养细胞的增殖、凋亡进程。

重度子痫前期患糊S6基因834＋7G／A和＋1332C／T多态性的研究

目的探讨生长停滞特异基因6（growth arrest specific gene6，GAS6）多态性是否与重度子痫前期发病有关联。方法采用聚合酶链反应一限制性片段长度多态性分析法对成都地区167例重度子痫前期患者和312名健康孕妇GAS6基因834＋7G／A和＋1332C／T多态性进行分析。结果GAS6基因＋1332C／T位点C、T等位基因的频率在重度子痫前期组为85．63％、14．37％，在正常孕妇组为78．04％、21．96％。＋1332C／T位点基因型和等位基因频率在重度子痫前期组和正常妊娠对照组之间差异具有统计学意义（均P〈0．05），CT＋TT基因型携带者在患者组的频率显著低于对照组（28．14％VS39．42％），发生重度子痫前期的风险降低（OR=0．602，95o．4CI：0．401～0．904，x^2=6．045，P=0．014）。834＋7G／A位点G、A等位基因的频率在重度子痫前期组为72．75％、27．25％，在正常孕妇组为74．36％、25．64％。两组之间基因型和等位基因频率差异无统计学意义（均P〉0．05）。此外，重度子痫前期组和正常妊娠对照组GAS6基因两位点基因型对血压水平未见显著影响。结论中国成都地区汉族人GAS6基因＋1332T等位基因与重度子痫前期发病的风险降低可能存在关联，但未见834＋7G／A位点与重度子痫前期的发生有关。