In the past 40 years,advances in neonatal intensive care unit(NICU)technology have enabled premature infants with lower birth weight and younger gestational age to survive.But with it comes an increase in the incidenc...In the past 40 years,advances in neonatal intensive care unit(NICU)technology have enabled premature infants with lower birth weight and younger gestational age to survive.But with it comes an increase in the incidence of long-term respiratory dysfunction,mainly in the form of bronchopulmonary dysplasia(BPD).Preventing lung injury is crucial for preventing BPD and improving the long-term prognosis of premature infants.Therefore,how to avoid ventilator-associated lung injury has become a focus of clinical and scientific research in premature infants in recent years.This article will elaborate on the susceptibility and pathophysiology of premature infant lung injury,ventilation strategies for preventing lung injury,and new advances in neonatal respiratory support.展开更多
Length and thickness of 152 corpus callosa Using ultrasonic diagnostic equipment with a were measured in neonates within 24 hours ot b^rtn. neonatal brain-specific probe, corpus callosum length and thickness of the ge...Length and thickness of 152 corpus callosa Using ultrasonic diagnostic equipment with a were measured in neonates within 24 hours ot b^rtn. neonatal brain-specific probe, corpus callosum length and thickness of the genu, body, and splenium were measured on the standard mid-sagittal plane, and the anteroposterior diameter of the genu was measured in the coronal plane. Results showed that corpus callosum length as well as thickness of the genu and splenium increased with gesta- tional age and birth weight, while other measures did not. These three factors on the standard mid-sagittal plane are therefore likely to be suitable for real-time evaluation of corpus callosum de- velopment in premature infants using cranial ultrasound. Further analysis revealed that thickness of the body and splenium and the anteroposterior diameter of the genu were greater in male infants than in female infants, suggesting that there are sex differences in corpus callosum size during the neonatal period. A second set of measurements were taken from 40 premature infants whose ges- tational age was 34 weeks or less. Corpus callosum measurements were corrected to a gestational age of 40 weeks, and infants were grouped for analysis depending on the outcome of a neonatal behavioral neurological assessment. Compared with infants with a normal neurological assessment, corpus callosum length and genu and splenium thicknesses were less in those with abnormalities, indicating that corpus callosum growth in premature infants is associated with neurobehavioral development during the early extrauterine stage.展开更多
Objective: Measure the effects of Early Intervention ad modum Katona (EI-K) in high-risk premature infants by means of clinical, neurobehavioral, and neurophysiologic tests. Method: We used the Amiel-Tison neurologic ...Objective: Measure the effects of Early Intervention ad modum Katona (EI-K) in high-risk premature infants by means of clinical, neurobehavioral, and neurophysiologic tests. Method: We used the Amiel-Tison neurologic examination, the Bayley Scale of Infant Behavior, and electroencephalography (EEG) recordings at 42 weeks of conceptional age, and after 6 months of treatment EI-K (n = 14) and compared these results with those of a group of infants without early intervention (nEI) (n = 11). Results: We found better performance of infants in EI-K than nEI group after 6 months of treatment in neurologic and behavioral examination measurements, but found no differences in EEG comparisons. Conclusion: Our data suggest significant benefit of the use of EI-K program over n-EI in the neurologic and neurobe-havior examinations of premature infants after 6 months of age.展开更多
Objective： To investigate the effect of tuina on promoting neurodevelopment of premature infants with brain injury. Methods： A total of 82 cases who met the inclusion criteria were randomized into an intervention g...Objective： To investigate the effect of tuina on promoting neurodevelopment of premature infants with brain injury. Methods： A total of 82 cases who met the inclusion criteria were randomized into an intervention group and a control group. In addition to early conventional intervention （Chinese medicinal bath therapy and physical training）, specific tuina manipulations （aims to unblock the Governor Vessel, refresh the brain, benefit the kidney and strengthen the spleen） were combined in the intervention group, whereas comforting touch in the control group. The rehabilitation assessments were then made prior to treatment and three months after the treatment using Gross Motor Function Measure （GMFM）, Bayley Scales of Infant Development-II （BSID-II） and Gesell Developmental Schedules （GDS） based Developmental Quotient （DQ）. Results： The DQ in all GDS areas and scores of GMFM A and B dimensions were significantly increased in both groups after the treatment （P〈0.01）. The DQ and population mean of GMFM B dimension in the intervention group were higher than those in the control group, showing statistical differences （P〈0.05）. However, in terms of Mental Development Index （MDI） and Physical Development Index （PDI） in both groups, there were no statistical differences before and after treatments. Conclusion： Both methods combined with conventional early intervention can help to improve the mental and motor development of infants with brain injury. However, the specific tuina manipulations have been proved more effective in improving the infants＇ gross motor function, particularly the ＂sitting＂ item.展开更多
Background and Objective: Head trauma in infants is a common health problem that has not been the focus of many research articles. The aim of study was to describe the epidemiology, etiology, management and clinical o...Background and Objective: Head trauma in infants is a common health problem that has not been the focus of many research articles. The aim of study was to describe the epidemiology, etiology, management and clinical outcome of head trauma in infants (<2 years) in a high flow tertiary hospital. Patients and Methods: This study was conducted on 95 consecutive infants with head trauma admitted to the neurotrauma unit in Cairo University hospitals during the period between September 2013 and December 2014. The data of these patients including age, sex, mode of trauma, neurological status on admission, CT findings, operative details in surgical cases, clinical outcome, and length of hospital stay were analyzed. Results: The study included 57 boys and 38 girls, with mean age of 13.2 months (range: 3 days - 23 months). The most common mode of trauma was falls (82.1%). Most of the patients had mild head injury (84.2%). Twenty two patients were operated upon, including 9 patients for evacuation of hematomas, 11 patients for elevation of depressed fractures, 1 patient for decompressive craniotomy and 1 patient for growing skull fracture. Good recovery followed in 83 patients. There were 4 deaths. Mean length of stay was 4 days (range: 1 - 31 days). Conclusion: Most of head injuries in infants are mild. The commonest etiology is falls, which is a preventable cause in many circumstances. The majority of these patients do not require surgical intervention. Good recovery is the rule in most of these infants with low mortality rates.展开更多
Background：Compared with full-term infants, very preterm infants are more vulnerable to injury and long-term disability and are at high risk of death. The predictive value of ultrasound and imaging on the neurodevelo...Background：Compared with full-term infants, very preterm infants are more vulnerable to injury and long-term disability and are at high risk of death. The predictive value of ultrasound and imaging on the neurodevelopment is one of the hot topics. This study aimed to investigate the relationship between cranial ultrasound （cUS） variables and neurodevelopmental outcomes of very preterm infants.Methods：Totally 129 very preterm infants （gestational age ≤28 weeks） in neonatal intensive care unit of Hunan Children’s Hospital between January 2012 and November 2014 were included in this retrospective study. Serial cUS （weekly before discharge and monthly after discharge） was performed on the infants until 6 months or older. Magnetic resonance imaging （MRI） was performed on the infants at approximately the term-equivalent age. The mental developmental index （MDI） and psychomotor developmental index （PDI） were followed up until the infants were 24 months or older. The relationship between brain injury and MDI/PDI scores was analyzed.Results：The consistency rate between cUS and MRI was 88%. At the first cUS, germinal matrix hemorrhage （GMH） Grades 3 and 4, hospitalization duration, and weight are significantly correlated with MDI/PDI and prognosis （MDI： odds ratio [OR] = 8.415, 0.982, and 0.042, P = 0.016, 0.000, and 0.004; PDI： OR = 7.149, 0.978, and 0.012, P = 0.025, 0.000, and 0.000, respectively）. At the last cUS, gestational age, extensive cystic periventricular leukomalacia （c-PVL）, and moderate and severe hydrocephaly are significantly correlated with MDI （OR = 0.292, 60.220, and 170.375, P = 0.004, 0.003, and 0.000, respectively）. Extensive c-PVL and moderate and severe hydrocephaly are significantly correlated with PDI （OR = 76.861 and 116.746, P = 0.003 and 0.000, respectively）.Conclusions：Very premature infants with GMH Grades 3 and 4, short hospitalization duration, and low weight have low survival rates and poorly developed brain nerves. Cerebral palsy can result from severe cerebral hemorrhage, moderate and severe hydrocephaly, and extensive c-PVL. The sustained, inhomogeneous echogenicity of white matter may suggest subtle brain injury.展开更多
Oligodendrocyte lineage gene 1 plays a key role in hypoxic-ischemic brain damage and myelin repair, miRNA-9 is involved in the occurrence of many related neurological disorders. Bioin- formatics analysis demonstrated ...Oligodendrocyte lineage gene 1 plays a key role in hypoxic-ischemic brain damage and myelin repair, miRNA-9 is involved in the occurrence of many related neurological disorders. Bioin- formatics analysis demonstrated that miRNA-9 complementarily, but incompletely, bound oligodendrocyte lineage gene 1, but whether miRNA-9 regulates oligodendrocyte lineage gene 1 remains poorly understood. Whole brain slices of 3-day-old Sprague-Dawley rats were cultured and divided into four groups： control group; oxygen-glucose deprivation group （treatment with 8% O2 ＋ 92% N2 and sugar-free medium for 60 minutes）; transfection control group （after oxygen and glucose deprivation for 60 minutes, transfected with control plasmid） and miRNA-9 transfection group （after oxygen and glucose deprivation for 60 minutes, transfected with miRNA-9 plasmid）. From the third day of transfection, and with increasing culture days, oligodendrocyte lineage gene 1 expression increased in each group, peaked at 14 days, and then decreased at 21 days. Real-time quantitative PCR results, however, demonstrated that oligoden- drocyte lineage gene 1 expression was lower in the miRNA-9 transfection group than that in the transfection control group at 1, 3, 7, 14, 21 and 28 days after transfection. Results suggested that miRNA-9 possibly negatively regulated oligodendrocyte lineage gene 1 in brain tissues during hypoxic-ischemic brain damage.展开更多
Background:High-mobility group box-1 (HMGB1) protein acts as an important pro-inflammatory mediator,which is capable of activating inflammation and tissue repair.HMGB1 can bind to its receptor such as advanced glycati...Background:High-mobility group box-1 (HMGB1) protein acts as an important pro-inflammatory mediator,which is capable of activating inflammation and tissue repair.HMGB1 can bind to its receptor such as advanced glycation end products (RAGE).RAGE,in turn,can promote the production of pro-inflammatory cytokines.Soluble RAGE (sRAGE) is a truncated form of the receptor comprising the extracellular domain of RAGE and can inhibit RAGE-activation.The objective of this study was to investigate whether HMGB1 and RAGE are involved in the development of brain injury in preterm infants.Methods:In total,108 infants ≤34 weeks gestation at birth were divided into 3 groups according to cranial altrasound scan:mild brain damage (n=33),severe brain damage (n=8) and no brain damage (n=67).All the placentas were submitted for pathologic evaluation.Histological chorioamnionitis (HCA) was defined as neutrophil infiltration of amniotic membranes,umbilical cord or chorionic plate.Expressions of HMGB1 and RAGE proteins were assessed by immunohistochemical analysis.The concentration of HMGB1 and sRAGE in umbilical cord blood were measured by enzyme-linked immunosorbent assay.Results:The frequency of HCA was 30.12%.HCA was associated with elevated concentrations of HMGB1 and decreased sRAGE in umbilical cord blood.The severe brain injury group demonstrated higher cord blood HMGB1 concentrations (P<0.001) and lower sRAGE concentrations (P<0.001) than both other groups.Brain injury in the premature infants was linked to intense staining for HMGB1/RAGE,particularly in inflammatory cells.Conclusions:Changes of cord blood HMGB1 and sRAGE of premature infants had direct relationship with the degree of inflammation and severity of brain damage.Monitoring sRAGE and HMGB1 levels may be helpful to predict intrauterine infection and brain injury in premature infants.展开更多
Oligodendrocyte lineage gene-1 expressed in oligodendrocytes may trigger the repair of neuronal myelin impairment, and play a crucial role in myelin repair. Hypoxia-inducible factor la, a transcription factor, is of g...Oligodendrocyte lineage gene-1 expressed in oligodendrocytes may trigger the repair of neuronal myelin impairment, and play a crucial role in myelin repair. Hypoxia-inducible factor la, a transcription factor, is of great significance in premature infants with hypoxic-ischemic brain damage There is little evidence of direct regulatory effects of hypoxia-inducible factor le on oligodendrocyte lineage gene-l. In this study, brain slices of Sprague-Dawley rats were cultured and subjected to oxygen-glucose deprivation. Then, slices were transfected with hypoxia-inducible factor la or oligodendrocyte lineage gene-1. The expression levels of hypoxia-inducible factor la and oligodendrocyte lineage gene-1 were significantly up-regulated in rat brains prior to transfection, as detected by immunohistochemical staining. Eight hours after transfection of slices with hypoxia-inducible factor la, oligodendrocyte lineage gene-1 expression was upregulated, and reached a peak 24 hours after transfection. Oligodendrocyte lineage gene-1 transfection induced no significant differences in hypoxia-inducible factor la levels in rat brain tissues with oxygen-glucose deprivation. These experimental findings indicate that hypoxia-inducible factor la can regulate oligodendrocyte lineage gene-1 expression in hypoxic brain tissue, thus repairing the neural impairment.展开更多
Objective： To investigate the effect of combined acupuncture and rehabilitation on intelligence and motor development of high risk infants with perinatal brain injuries. Methods： Ninety-seven survived cases in the n...Objective： To investigate the effect of combined acupuncture and rehabilitation on intelligence and motor development of high risk infants with perinatal brain injuries. Methods： Ninety-seven survived cases in the neonatal intensive care unit （NICU） were allocated into a treatment group of 53 cases and a control group of 44 cases following the consent of the parents. Cases in the treatment group were treated by combined acupuncture and rehabilitation; whereas cases in the control group were treated by conventional child care methods. Systemic follow-up has been made for 2 years. Then the infants＇ intelligence was evaluated respectively in the 6th, 12th and 34th months. Results： Compared with the control group, the mental development indexes （MDI） and physical development indexes （PDI） in the treatment group were increased by 14.96 and 9.82 respectively. There was a significant difference （χ2=8.1659, P〈0.01 ） in the abnormal intelligence rates between the treatment group （15.1%） and the control group （40.9%,）. Conclusion： Combined acupuncture and rehabilitation can effectively improve the mental and physical development of high-risk infants with perinatal brain injuries. Additionally, this therapy can decrease the disability rates and increase the infants＇ quality of life.展开更多
文摘In the past 40 years,advances in neonatal intensive care unit(NICU)technology have enabled premature infants with lower birth weight and younger gestational age to survive.But with it comes an increase in the incidence of long-term respiratory dysfunction,mainly in the form of bronchopulmonary dysplasia(BPD).Preventing lung injury is crucial for preventing BPD and improving the long-term prognosis of premature infants.Therefore,how to avoid ventilator-associated lung injury has become a focus of clinical and scientific research in premature infants in recent years.This article will elaborate on the susceptibility and pathophysiology of premature infant lung injury,ventilation strategies for preventing lung injury,and new advances in neonatal respiratory support.
基金supported by the Hebei Province Population and the Family Planning Commission of Science and Technology Research Program in China,No.2008-B04
文摘Length and thickness of 152 corpus callosa Using ultrasonic diagnostic equipment with a were measured in neonates within 24 hours ot b^rtn. neonatal brain-specific probe, corpus callosum length and thickness of the genu, body, and splenium were measured on the standard mid-sagittal plane, and the anteroposterior diameter of the genu was measured in the coronal plane. Results showed that corpus callosum length as well as thickness of the genu and splenium increased with gesta- tional age and birth weight, while other measures did not. These three factors on the standard mid-sagittal plane are therefore likely to be suitable for real-time evaluation of corpus callosum de- velopment in premature infants using cranial ultrasound. Further analysis revealed that thickness of the body and splenium and the anteroposterior diameter of the genu were greater in male infants than in female infants, suggesting that there are sex differences in corpus callosum size during the neonatal period. A second set of measurements were taken from 40 premature infants whose ges- tational age was 34 weeks or less. Corpus callosum measurements were corrected to a gestational age of 40 weeks, and infants were grouped for analysis depending on the outcome of a neonatal behavioral neurological assessment. Compared with infants with a normal neurological assessment, corpus callosum length and genu and splenium thicknesses were less in those with abnormalities, indicating that corpus callosum growth in premature infants is associated with neurobehavioral development during the early extrauterine stage.
文摘Objective: Measure the effects of Early Intervention ad modum Katona (EI-K) in high-risk premature infants by means of clinical, neurobehavioral, and neurophysiologic tests. Method: We used the Amiel-Tison neurologic examination, the Bayley Scale of Infant Behavior, and electroencephalography (EEG) recordings at 42 weeks of conceptional age, and after 6 months of treatment EI-K (n = 14) and compared these results with those of a group of infants without early intervention (nEI) (n = 11). Results: We found better performance of infants in EI-K than nEI group after 6 months of treatment in neurologic and behavioral examination measurements, but found no differences in EEG comparisons. Conclusion: Our data suggest significant benefit of the use of EI-K program over n-EI in the neurologic and neurobe-havior examinations of premature infants after 6 months of age.
基金supported by Guangdong ProvincialNatural Science Foundation (5002028)
文摘Objective： To investigate the effect of tuina on promoting neurodevelopment of premature infants with brain injury. Methods： A total of 82 cases who met the inclusion criteria were randomized into an intervention group and a control group. In addition to early conventional intervention （Chinese medicinal bath therapy and physical training）, specific tuina manipulations （aims to unblock the Governor Vessel, refresh the brain, benefit the kidney and strengthen the spleen） were combined in the intervention group, whereas comforting touch in the control group. The rehabilitation assessments were then made prior to treatment and three months after the treatment using Gross Motor Function Measure （GMFM）, Bayley Scales of Infant Development-II （BSID-II） and Gesell Developmental Schedules （GDS） based Developmental Quotient （DQ）. Results： The DQ in all GDS areas and scores of GMFM A and B dimensions were significantly increased in both groups after the treatment （P〈0.01）. The DQ and population mean of GMFM B dimension in the intervention group were higher than those in the control group, showing statistical differences （P〈0.05）. However, in terms of Mental Development Index （MDI） and Physical Development Index （PDI） in both groups, there were no statistical differences before and after treatments. Conclusion： Both methods combined with conventional early intervention can help to improve the mental and motor development of infants with brain injury. However, the specific tuina manipulations have been proved more effective in improving the infants＇ gross motor function, particularly the ＂sitting＂ item.
文摘Background and Objective: Head trauma in infants is a common health problem that has not been the focus of many research articles. The aim of study was to describe the epidemiology, etiology, management and clinical outcome of head trauma in infants (<2 years) in a high flow tertiary hospital. Patients and Methods: This study was conducted on 95 consecutive infants with head trauma admitted to the neurotrauma unit in Cairo University hospitals during the period between September 2013 and December 2014. The data of these patients including age, sex, mode of trauma, neurological status on admission, CT findings, operative details in surgical cases, clinical outcome, and length of hospital stay were analyzed. Results: The study included 57 boys and 38 girls, with mean age of 13.2 months (range: 3 days - 23 months). The most common mode of trauma was falls (82.1%). Most of the patients had mild head injury (84.2%). Twenty two patients were operated upon, including 9 patients for evacuation of hematomas, 11 patients for elevation of depressed fractures, 1 patient for decompressive craniotomy and 1 patient for growing skull fracture. Good recovery followed in 83 patients. There were 4 deaths. Mean length of stay was 4 days (range: 1 - 31 days). Conclusion: Most of head injuries in infants are mild. The commonest etiology is falls, which is a preventable cause in many circumstances. The majority of these patients do not require surgical intervention. Good recovery is the rule in most of these infants with low mortality rates.
文摘Background：Compared with full-term infants, very preterm infants are more vulnerable to injury and long-term disability and are at high risk of death. The predictive value of ultrasound and imaging on the neurodevelopment is one of the hot topics. This study aimed to investigate the relationship between cranial ultrasound （cUS） variables and neurodevelopmental outcomes of very preterm infants.Methods：Totally 129 very preterm infants （gestational age ≤28 weeks） in neonatal intensive care unit of Hunan Children’s Hospital between January 2012 and November 2014 were included in this retrospective study. Serial cUS （weekly before discharge and monthly after discharge） was performed on the infants until 6 months or older. Magnetic resonance imaging （MRI） was performed on the infants at approximately the term-equivalent age. The mental developmental index （MDI） and psychomotor developmental index （PDI） were followed up until the infants were 24 months or older. The relationship between brain injury and MDI/PDI scores was analyzed.Results：The consistency rate between cUS and MRI was 88%. At the first cUS, germinal matrix hemorrhage （GMH） Grades 3 and 4, hospitalization duration, and weight are significantly correlated with MDI/PDI and prognosis （MDI： odds ratio [OR] = 8.415, 0.982, and 0.042, P = 0.016, 0.000, and 0.004; PDI： OR = 7.149, 0.978, and 0.012, P = 0.025, 0.000, and 0.000, respectively）. At the last cUS, gestational age, extensive cystic periventricular leukomalacia （c-PVL）, and moderate and severe hydrocephaly are significantly correlated with MDI （OR = 0.292, 60.220, and 170.375, P = 0.004, 0.003, and 0.000, respectively）. Extensive c-PVL and moderate and severe hydrocephaly are significantly correlated with PDI （OR = 76.861 and 116.746, P = 0.003 and 0.000, respectively）.Conclusions：Very premature infants with GMH Grades 3 and 4, short hospitalization duration, and low weight have low survival rates and poorly developed brain nerves. Cerebral palsy can result from severe cerebral hemorrhage, moderate and severe hydrocephaly, and extensive c-PVL. The sustained, inhomogeneous echogenicity of white matter may suggest subtle brain injury.
基金supported by the National Natural Science Foundation of China,No.81241022the Beijing Municipal Natural Science Foundation in China,No.7122045,7072023
文摘Oligodendrocyte lineage gene 1 plays a key role in hypoxic-ischemic brain damage and myelin repair, miRNA-9 is involved in the occurrence of many related neurological disorders. Bioin- formatics analysis demonstrated that miRNA-9 complementarily, but incompletely, bound oligodendrocyte lineage gene 1, but whether miRNA-9 regulates oligodendrocyte lineage gene 1 remains poorly understood. Whole brain slices of 3-day-old Sprague-Dawley rats were cultured and divided into four groups： control group; oxygen-glucose deprivation group （treatment with 8% O2 ＋ 92% N2 and sugar-free medium for 60 minutes）; transfection control group （after oxygen and glucose deprivation for 60 minutes, transfected with control plasmid） and miRNA-9 transfection group （after oxygen and glucose deprivation for 60 minutes, transfected with miRNA-9 plasmid）. From the third day of transfection, and with increasing culture days, oligodendrocyte lineage gene 1 expression increased in each group, peaked at 14 days, and then decreased at 21 days. Real-time quantitative PCR results, however, demonstrated that oligoden- drocyte lineage gene 1 expression was lower in the miRNA-9 transfection group than that in the transfection control group at 1, 3, 7, 14, 21 and 28 days after transfection. Results suggested that miRNA-9 possibly negatively regulated oligodendrocyte lineage gene 1 in brain tissues during hypoxic-ischemic brain damage.
文摘Background:High-mobility group box-1 (HMGB1) protein acts as an important pro-inflammatory mediator,which is capable of activating inflammation and tissue repair.HMGB1 can bind to its receptor such as advanced glycation end products (RAGE).RAGE,in turn,can promote the production of pro-inflammatory cytokines.Soluble RAGE (sRAGE) is a truncated form of the receptor comprising the extracellular domain of RAGE and can inhibit RAGE-activation.The objective of this study was to investigate whether HMGB1 and RAGE are involved in the development of brain injury in preterm infants.Methods:In total,108 infants ≤34 weeks gestation at birth were divided into 3 groups according to cranial altrasound scan:mild brain damage (n=33),severe brain damage (n=8) and no brain damage (n=67).All the placentas were submitted for pathologic evaluation.Histological chorioamnionitis (HCA) was defined as neutrophil infiltration of amniotic membranes,umbilical cord or chorionic plate.Expressions of HMGB1 and RAGE proteins were assessed by immunohistochemical analysis.The concentration of HMGB1 and sRAGE in umbilical cord blood were measured by enzyme-linked immunosorbent assay.Results:The frequency of HCA was 30.12%.HCA was associated with elevated concentrations of HMGB1 and decreased sRAGE in umbilical cord blood.The severe brain injury group demonstrated higher cord blood HMGB1 concentrations (P<0.001) and lower sRAGE concentrations (P<0.001) than both other groups.Brain injury in the premature infants was linked to intense staining for HMGB1/RAGE,particularly in inflammatory cells.Conclusions:Changes of cord blood HMGB1 and sRAGE of premature infants had direct relationship with the degree of inflammation and severity of brain damage.Monitoring sRAGE and HMGB1 levels may be helpful to predict intrauterine infection and brain injury in premature infants.
基金supported by the National Natural Science Foundation of China,No. 81241022the Natural Science Foundation of Beijing,No. 7072023,7122045
文摘Oligodendrocyte lineage gene-1 expressed in oligodendrocytes may trigger the repair of neuronal myelin impairment, and play a crucial role in myelin repair. Hypoxia-inducible factor la, a transcription factor, is of great significance in premature infants with hypoxic-ischemic brain damage There is little evidence of direct regulatory effects of hypoxia-inducible factor le on oligodendrocyte lineage gene-l. In this study, brain slices of Sprague-Dawley rats were cultured and subjected to oxygen-glucose deprivation. Then, slices were transfected with hypoxia-inducible factor la or oligodendrocyte lineage gene-1. The expression levels of hypoxia-inducible factor la and oligodendrocyte lineage gene-1 were significantly up-regulated in rat brains prior to transfection, as detected by immunohistochemical staining. Eight hours after transfection of slices with hypoxia-inducible factor la, oligodendrocyte lineage gene-1 expression was upregulated, and reached a peak 24 hours after transfection. Oligodendrocyte lineage gene-1 transfection induced no significant differences in hypoxia-inducible factor la levels in rat brain tissues with oxygen-glucose deprivation. These experimental findings indicate that hypoxia-inducible factor la can regulate oligodendrocyte lineage gene-1 expression in hypoxic brain tissue, thus repairing the neural impairment.
文摘Objective： To investigate the effect of combined acupuncture and rehabilitation on intelligence and motor development of high risk infants with perinatal brain injuries. Methods： Ninety-seven survived cases in the neonatal intensive care unit （NICU） were allocated into a treatment group of 53 cases and a control group of 44 cases following the consent of the parents. Cases in the treatment group were treated by combined acupuncture and rehabilitation; whereas cases in the control group were treated by conventional child care methods. Systemic follow-up has been made for 2 years. Then the infants＇ intelligence was evaluated respectively in the 6th, 12th and 34th months. Results： Compared with the control group, the mental development indexes （MDI） and physical development indexes （PDI） in the treatment group were increased by 14.96 and 9.82 respectively. There was a significant difference （χ2=8.1659, P〈0.01 ） in the abnormal intelligence rates between the treatment group （15.1%） and the control group （40.9%,）. Conclusion： Combined acupuncture and rehabilitation can effectively improve the mental and physical development of high-risk infants with perinatal brain injuries. Additionally, this therapy can decrease the disability rates and increase the infants＇ quality of life.
