In order to find suspension culture conditions suitable for Premna microphylla Turea, with the leaves of experimented P. microphylla as a material, em- bryogenic callus induction, subculture, dispersion of embryogenic...In order to find suspension culture conditions suitable for Premna microphylla Turea, with the leaves of experimented P. microphylla as a material, em- bryogenic callus induction, subculture, dispersion of embryogenic callus and suspension culture were performed. The results showed that the calli induced from leaves were different. The medium most suitable for induction of loose embryogenic callus for suspension culture was MS ＋ 0.4 mg/L 2,4-D ＋ 0.4 mg/L NAA ＋ 3% sucrose ＋0.8% agar. A better cell line could be obtained with cellulase and pectinase for dispersion of P. microphyUa callus. The medium most suitable for sus- pension culture was MS ＋0.4 mg/L 2,4-D ＋0.8 mg/L KT ＋3% sucrose. In this medium, the fresh weight of the suspensian-cultured cells increased by 6.46 times per 7 d. Dark culture was more suitable for cell proliferation. The average contents of pectin and protein were, respectively, 6.57% and 0.12% in the sus- pended ceils of P. microphylla, which had been cultured for 3 weeks under the optimum culture conditions.展开更多
Nutritional components in leaves of Premna microphylla Turcz. were analyzed. Compared with the major crops and forages in China, its nutritional value was evaluated. The contents of crude protein and total amino a...Nutritional components in leaves of Premna microphylla Turcz. were analyzed. Compared with the major crops and forages in China, its nutritional value was evaluated. The contents of crude protein and total amino acid in leaves of P. microphylla were 134.8 and 108.1 mg/g respectively. It was rich in contents of Vc (3.6 mg/g), βcarotene (0.2 mg/g) and mineral elements. So this species is a wild plant resources which can be utilized widely.展开更多
研究了酸解法从豆腐柴叶中提取果胶的动力学过程,建立了受溶解果胶分子降解影响的果胶提取动力学模型,并用数学方法确定了最大的得率和最佳的提取时间。模型可应用于描述在实验室条件下,60、70、80和90℃时用盐酸从豆腐柴叶中提取果胶...研究了酸解法从豆腐柴叶中提取果胶的动力学过程,建立了受溶解果胶分子降解影响的果胶提取动力学模型,并用数学方法确定了最大的得率和最佳的提取时间。模型可应用于描述在实验室条件下,60、70、80和90℃时用盐酸从豆腐柴叶中提取果胶的酸提取过程,确立了提取过程的速率常数,从60℃下的3.01×10-4s-1变化到90℃下的8.64×10-4s-1,应用该模型对豆腐柴叶果胶的提取得率进行了理论计算,获得了90℃下最佳提取时间(50.19 m in)时的最大得率(8.007%),模型计算结果与实验数据吻合较好。所提出的理论模型能充分描述在酸性条件下的果胶提取过程和预测果胶的最大得率、果胶降解量和最佳的提取时间。展开更多
优化豆腐柴叶豆腐加工工艺及配方,为豆腐柴的开发利用提供科学依据。采用单因素试验、正交试验等方法研究豆腐柴叶豆腐的制备工艺和配方,并通过感官评价研究各个因素交互作用对其豆腐品质的影响,从而确定豆腐柴叶豆腐最佳加工工艺和配...优化豆腐柴叶豆腐加工工艺及配方,为豆腐柴的开发利用提供科学依据。采用单因素试验、正交试验等方法研究豆腐柴叶豆腐的制备工艺和配方,并通过感官评价研究各个因素交互作用对其豆腐品质的影响,从而确定豆腐柴叶豆腐最佳加工工艺和配方。豆腐柴叶豆腐的最佳加工工艺为:水添加量20%、水温50℃、桐壳灰添加量0.09%、p H值7。在该最佳工艺条件下豆腐柴叶豆腐感官评分93.2,平均出品率92.43%,平均浸出液体积17.4 m L。该工艺条件下的豆腐柴叶豆腐口感鲜美、清凉消暑,具有良好的食用品质和一定的保健功能。展开更多
基金Supported by Nature Science Foundation of Hubei Province(2008CDZ085)
文摘In order to find suspension culture conditions suitable for Premna microphylla Turea, with the leaves of experimented P. microphylla as a material, em- bryogenic callus induction, subculture, dispersion of embryogenic callus and suspension culture were performed. The results showed that the calli induced from leaves were different. The medium most suitable for induction of loose embryogenic callus for suspension culture was MS ＋ 0.4 mg/L 2,4-D ＋ 0.4 mg/L NAA ＋ 3% sucrose ＋0.8% agar. A better cell line could be obtained with cellulase and pectinase for dispersion of P. microphyUa callus. The medium most suitable for sus- pension culture was MS ＋0.4 mg/L 2,4-D ＋0.8 mg/L KT ＋3% sucrose. In this medium, the fresh weight of the suspensian-cultured cells increased by 6.46 times per 7 d. Dark culture was more suitable for cell proliferation. The average contents of pectin and protein were, respectively, 6.57% and 0.12% in the sus- pended ceils of P. microphylla, which had been cultured for 3 weeks under the optimum culture conditions.
文摘Nutritional components in leaves of Premna microphylla Turcz. were analyzed. Compared with the major crops and forages in China, its nutritional value was evaluated. The contents of crude protein and total amino acid in leaves of P. microphylla were 134.8 and 108.1 mg/g respectively. It was rich in contents of Vc (3.6 mg/g), βcarotene (0.2 mg/g) and mineral elements. So this species is a wild plant resources which can be utilized widely.
文摘研究了酸解法从豆腐柴叶中提取果胶的动力学过程,建立了受溶解果胶分子降解影响的果胶提取动力学模型,并用数学方法确定了最大的得率和最佳的提取时间。模型可应用于描述在实验室条件下,60、70、80和90℃时用盐酸从豆腐柴叶中提取果胶的酸提取过程,确立了提取过程的速率常数,从60℃下的3.01×10-4s-1变化到90℃下的8.64×10-4s-1,应用该模型对豆腐柴叶果胶的提取得率进行了理论计算,获得了90℃下最佳提取时间(50.19 m in)时的最大得率(8.007%),模型计算结果与实验数据吻合较好。所提出的理论模型能充分描述在酸性条件下的果胶提取过程和预测果胶的最大得率、果胶降解量和最佳的提取时间。
文摘优化豆腐柴叶豆腐加工工艺及配方,为豆腐柴的开发利用提供科学依据。采用单因素试验、正交试验等方法研究豆腐柴叶豆腐的制备工艺和配方,并通过感官评价研究各个因素交互作用对其豆腐品质的影响,从而确定豆腐柴叶豆腐最佳加工工艺和配方。豆腐柴叶豆腐的最佳加工工艺为:水添加量20%、水温50℃、桐壳灰添加量0.09%、p H值7。在该最佳工艺条件下豆腐柴叶豆腐感官评分93.2,平均出品率92.43%,平均浸出液体积17.4 m L。该工艺条件下的豆腐柴叶豆腐口感鲜美、清凉消暑,具有良好的食用品质和一定的保健功能。
