Integrated Use of Alternative Methods for Evaluating the Skin Sensitization Potencies of Five Frequently Used Preservatives in Cosmetics

In vitro skin sensitization testing methods based on the adverse outcome pathway(AOP)were used to evaluate the skin sensitization potencies of 5 commonly used preservatives.According to the“2 out of 3”principle of the integrated approaches to testing and assessment(IATA)the direct peptide reactivity assay(DPRA)and the human cell line activation test(h-CLAT)were used to detect the preservatives commonly used in cosmetics,including phenoxyethanol.methyl paraben,propyl paraben,imidazolidinyl urea and DMDM hydantoin.The DPRA and the h-CLA were carried out according to the OEC442C and 442E guidelines,respectively.The results show that.phenoxyethanol and methyl paraben are both negative in DPRA and h-CLAT while imidazolidinyl urea and DMDM hydantoin are both positive in these two tests.Propyl paraben has negative result in DPRA but positive result in h-CLAT.Therefore,imidazolidiny urea and DMDM hydantoin are sensitizers,while phenoxyethanol and methylparaben are non-sensitizers.Taken animal and human data into consideration,it is predicted that propyl paraben should be a non-sensitizer.The combination of DPRA and h-CLAT can make up for the limitations of using a single method,and it is suitable for the preliminary screening of cosmetic raw materials according to skin sensitization.

Comparative clinical study of conjunctival toxicities of newer generation fluoroquinolones without the influence of preservatives

AIMTo compare the conjunctival epithelial toxicities of three newer-generation fluoroquinolones without preservatives.METHODSIn a prospective, randomized, double blind comparative study, 47 eyes of 47 patients with a primary pterygium were enrolled, and divided randomly into three groups (levofloxacin 0.5%, gatifloxacin 0.3%, and moxifloxacin 0.5%). After pterygium surgery with the same conjunctival autograft technique, each patient maintained a regimen with a randomly assigned fluoroquinolone eye drop. Patients were examined every other day after surgery until the epithelium had completely healed. Photos were taken and used to measure the area of residual epithelial defects. Conjunctival healing time and speed (initial defect area/healing time (mm2/d) compared in each group using Kruskal-Wallis tests.RESULTSThere were no significant differences in mean age, gender, and conjunctival defect size of the donor site between these groups. However, the mean of conjunctival healing time and speed were statistically different in each group. The mean of conjunctival epithelial healing time was 8.93±2.69d (levofloxacin group), 10.31±2.96d (gatifloxacin group), and 13.50±4.10d (moxifloxacin group), P=0.006. The mean conjuctival epithelial healing speed was 6.18±1.39 mm2/d (levofloxacin group), 5.52±1.68 mm2/d (gatifloxacin group), and 4.40±1.30 mm2/d (moxifloxacin group), P=0. 003.CONCLUSIONWithout the influence of preservatives, levofloxacin and gatifloxacin might be less toxic to the regeneration of conjunctival epithelial cells and cause a faster conjunctival wound healing relative to moxifloxacin.

Determination of Malaysian Herbs and Spices as Biopreservative Agents in Food Products

Preservatives are usually added to food products to ensure longer shelf life and prevent decomposition process and microbial growth. However, synthetic food preservatives can also give negative side effect to health and are harmful to human and animal physiology. Based on the potential of herbs and spices as antimicrobial agent, the purpose of this study is to identify antibacterial activity from extracts of some local herbs and spices: Phaeomeria speciosa (P. speciosa), Aquilaria subintegra (A. subintegra), Polygonum minus (P. minus), Syzygium aromaticum (S. aromaticum), Cinnamomum verum (C. verum) and Piper nigrum (P. nigrum) against food bacteria using disc diffusion method. Results revealed that dichloromethane extracts of C. verum, hexane extracts of S. aromaticum and P. minus showed the most active antibacterial against tested bacteria. The Minimal Inhibitory Concentrations (MIC) ranged from 25 to 75 mg/ml for dichloromethane extract of C. verum, hexane extract of S. aromaticum and P. minus. Therefore further research should be pursued to identify the chemical structure of antibacterial agents from the active extracts as an alternative source of natural preservatives.

Terminalia arjuna:A novel natural preservative for improved lipid oxidative stability and storage quality of muscle foods

The study was conducted to explore the possibility of utilization of Terminalia arjuna as a novel natural preservative in meat products by using chevon sausages as a model system.Chevon sausages were prepared by incorporating different levels of T.arjuna viz.T1(0.25%),T2(0.50%)and T3(0.75%)and were assessed for various lipid oxidative stability and storage quality parameters under refrigerated(4±1◦C)conditions.T.arjuna showed a significant(p<0.05)effect on the lipid oxidative stability as the treated products exhibited significantly(p<0.05)lower TBARS(mg malonaldehyde/kg)values in comparison to control.A significant(p<0.05)effect was also observed on the microbial stability as T.arjuna incorporated products showed significantly(p<0.05)lower values for total plate count(log cfu/g),psychrophilic count(log cfu/g),yeast and mould count(log cfu/g)and FFA(%oleic acid)values.Significantly(p<0.05)higher scores were observed for various sensory parameters of the products incorporated with T.arjuna during refrigerated storage.T.arjuna successfully improved the lipid oxidative stability and storage quality of the model meat product and may be commercially exploited as a novel preservative in muscle foods.

Simultaneous spectrophotometric determination of four preservatives in foodstuffs by multivariate calibration and artificial neural networks

Benzoic acid （BA）, methylparaben （MP）, propylparaben （PP） and sorbic acid （SA） are food preservatives, and they have well defined UV spectra. However, their spectra overlap seriously, and it is difficult to determine them individually from their mixtures without preseparation. In this paper, seven different chemometric approaches were applied to resolve the overlapping spectra and to determine these compounds simultaneously. With respect to the criteria of % relative prediction error （RPE） and % recovery, principal component regression （PCR） and radial basis function-artificial neural network （RBF-ANN） were the preferred methods. These two methods were successfully applied to the analysis of some commercial samples.

An Application of Gas Chromatography-Mass Spectrometry (GC-MS) Fast Automated Scan/SIM Type (FASST) in Determining the Preservative Levels in Foods

The preservatives used in food products are strictly monitored and regulated. The present study aims to imply a sensitive and reliable analytical method to quantify two classes of preservatives (i.e. carboxylic acid and phenolic compounds) in food by means of gas chromatography-mass spectrometry (GC-MS), aimed at monitoring the products available within the local stores. Target analytes were derivatized via aqueous-phase isobutyl chloroformate-mediated reaction followed by dispersive liquid-liquid microextraction (DLLME) method. The quantity and quality determination of the studied samples were assured by performing FASST approach in a single run GC-MS analysis. The combination of standard addition method with sample dilution compensated the effect of sample matrix on the quantitative determination of the tested preservatives in the examined samples. The concentrations of sorbic acid (SA) were 210 μg/mL and 1000 μg/mL in soft drink and sauces samples, respectively. On the other hand, sodium benzoate (226 μg/mL) was only found in soft drink, whereas, no parabens were detected in any samples collected from the local stores.

Study of Weak Acid Preservatives and Modified Atmosphere Packaging (MAP) on Mold Growth in Modal Agar System

Inhibition of spoilage mold of common contaminant of bakery goods in modal agar system by potassium sorbate and calcium propionate in wide range of concentration (0 - 2000 ppm) at different pH (5.5 - 7) was investigated. All Samples were examined daily for mold growth. Results showed that both potassium sorbate and calcium propionate can be used effectively to inhibit mold growth and the effectiveness of these preservatives is enhanced by increasing the concentration and decreasing the pH. The effect of modified atmosphere packaging (MAP) comprised of 60% or 80% CO2 (balance N2) and oxygen absorbents on the growth of mold was also studied on the agar system. The results indicated that mold growth was prevented up to 42 days in packs flushed with 60% or 80% CO2 (balance N2). It was also evident that all samples did not show mold growth for >40 days when packaged in the presence of oxygen scavenger sachet.

Tannin Resins for Wood Preservatives:A Review

Tannins and wood preservatives,in this article,are briefly introduced at beginning.The research and application progress on tannin resins for wood preservatives at home and abroad are reviewed.The significance and development prospects of research on tannins for wood preservatives are prospected.

Application of Hyperspectral Imaging Technology in Rapid Detection of Preservative in Milk

To ensure the quality and safety of pure milk,detection method of typical preservative-potassium sorbate in milk was researched in this paper.Hyperspectral imaging technology was applied to realize rapid detection.Influence factors for hyperspectral data collection for milk samples were firstly researched,including height of sample,bottom color and sample filled up container or not.Pretreatment methods and variable selection algorithms were applied into original spectral data.Rapid detection models were built based on support vector machine method(SVM).Finally,standard normalized variable(SNV)-competitive adaptive reweighted sampling(CARS)and SVM model was chosen in this paper.The accuracies of calibration set and testing set were 0.97 and 0.97,respectively.Kappa coefficient of the model was 0.93.It could be seen that hyperspectral imaging technology could be used to detect for potassium sorbate in milk.Meanwhile,it also provided methodological supports for the rapid detection of other preservatives in milk.

Assessment of Cytological Effects of Food Preservative Potassium Metabisulphite to <i>Allium cepa</i>

With the unprecedented development of the food industry, food preservatives have gained a leading role in food processing. In this study, investigations were carried out to assess the cytological effects of potassium metabisulphite (PMB) to onion (Allium cepa), one of the most used plants for determining the cytotoxic and genotoxic effects of different chemicals. Meristematic roots of A. cepa were treated with PMB solutions in different concentrations, ranging from 10 mg/l to 35 mg/l for 6, 12 and 24 h alongside an untreated control. The quantified parameters for the different concentrations of the PMB were mitotic index (MI %), mitosis phases index (PI %) and total abnormalities index (TAI %) meaning chromosomal aberrations and nuclear abnormalities. The results indicated that PMB reduced MI in A. cepa with increasing the concentrations and time exposure as compared with the untreated control. Thus, at concentration of 10 - 35 mg/l PMB, these values were reduced from 8.34% to 4.81% (6 h);7.18% to 2.35% (12 h) and 4.12 to 1.43 (24 h). Also, the TAI value increased with increasing PMB concentrations and time: 0.51% to 9.98% (6 h), 7.11% to 19.84% (12 h) and 17.79 to 41.21 (24 h). The types of abnormalities induced by PMB in A. cepa meristematic cells were micronucleus, C-metaphase, star anaphase, stickiness, laggards, fragments, binucleated cells and pulverised nucleus. These alarming findings indicate the cytotoxic and genotoxic effect of PMB to A. cepa and suggest necessity to adopt more natural alternatives for food preservation in the future.

Inhibition Effect of Herbal Preservatives on Listeria monocytogenes on Chilled Pork

This study investigated the growth situation of Listeria monocytogenes on chilled pork and the effect of herbal preservatives on this pathogen. The inhibitions of herbal preservatives were identified. The minimum inhibitory concentrations （MIC） of cinnamon and clove were all 0.79 mg.mL^-1, while the rosemary was 1.58 mg.mL^-1. And the composite herbal preservatives were got through orthogonal experiment. The optimum proportion was as following on agar medium： 1.16 mg.mL^-1 cinnamon＋2.38 mg. mL^-1 rosemary＋3.17 mg. mLl clove （herb combination number 5）, while on chilled pork, the strong inhibition of L. monocytogenes was showed, which demonstrated that the surface application of herb combination resulted in an effective delay of L. monocytogenes growth.

Chondrocyte viability depends on the preservative solution

Fresh osteochondral grafts find broad application in the treatment of extensive and focal damages of joint surfaces. The maintenance of chondrocyte viability of the collected grafts is of key importance. Aim: The evaluation of chondrocyte survivability in a stable temperature of 40C in different preservative solutions is the aim of this work. Method: Chondrocyte survivability has been evaluated in saline solution (group I), Ringer solution (group II), saline with an addition of hyaluronic acid (group III) and saline enriched with glucosamine sulphate (group IV). The amount of live chondrocytes was examined on the day of collection and subsequently after 1, 2, 3,6,12, and 21 days using the Promega MTT test. Results: The highest number of live chondrocytes as calculated for 1g of hyaline cartilage after 21 days was ascertained in group IV (saline with glucosamine sulphate). The lowest number of live chondral cells was observed in group II (saline with hyaluronic acid). Chondrocyte survivability in saline (group I) was higher than in the Ringer solution (group II). Conclusions: The enrichment of saline solution with glucosamine sulphate protracts the viability of chondrocytes in fresh osteochondral gra- fts prepared for chondral transplantation.

Effect of Sodium Chloride on Subsequent Survival of Staphylococcus aureus in Various Preservatives

Chemical preservatives in foods are nowadays added at lower concentrations. However, this may allow survival of bacterial cells and induce increased resistance to various preservatives. In this study, the effects of growth in NaCl (10% or 15%) on survival of Staphylococcus aureus strains in various chemical and physical preservatives were investigated. Growth of the strains for 20 h at 37°C in nutrient broth containing 10% NaCl enhanced survival in chemical preservatives (e.g. nutrient broth containing 20% NaCl, or 0.3% thyme extract, or 0.1% ascorbic acid). Growth at 37°C for 20 h in nutrient broth containing 15% NaCl or for 5 d in nutrient broth containing 10% NaCl greatly enhanced survival of the strains in the tested preservatives. For survival at low temperature (5°C) (physical preservative), cells grown at 37°C for 20 h in nutrient broth containing 10% NaCl were not more tolerant to low temperature. Growth of the strains at 37°C for 20 h in nutrient broth containing 15% NaCl or for 5 d in nutrient broth containing 10% NaCl only slightly increased the survival of cells at low temperature.

Study on the Adsorption of Preservatives by Eyeliner Pen Packaging Materials

In order to study adsorption of preservatives by eyeliner pen package,the chemical compositions of the commercially available Eyeliner package were analyzed by infrared spectrometer.It was found that these package components were PP,PET,modified PP,modified PET,ABS,nylon,PU and so on.The adsorption effects of these components on three preservatives,i.e.phenoxyethanol,Methyl paraben and Propyl paraben have been further studied by high performance liquid chromatography.The results demonstrated that the four chemical materials PET,PP,modified PP and ABS had no adsorption on the above three preservatives.However,the component named of water diversion core which was composed of modified PET,and nylon-made cotton head have been observed of slight adsorption in this study.And components as the function of preventing liquid leakage,which was made of PU,have presented the most serious adsorption on these three preservatives,with the adsorption order as Propyl Paraben>Methyl Paraben>phenoxyethanol.

An Exploratory Analysis of the Market Perspective on Reclaiming Chromated Copper Arsenate (CCA) from Decommissioned Preservative-Treated Wood Utility Poles

In the area of recycling of spent chromated copper arsenate (CCA)-treated wood, most studies to date have focused on methods of removing/extracting the residual preservative from the wood matrix. It is well recognized that exposure of CCA-treated wood to an acid solution can reverse the CCA fixation process thereby converting the CCA elements into their water-soluble form. The economic viability of the process is enhanced because it can be integrated with other technologies and products (e.g., “green” spray foam insulation, etc.). The market for the “green” CCA is the same as for traditional CCA-the wood treating industry, principally utility poles and pilings. A market research study was conducted to determine the suitability of spent CCA-treated wood as a source for recycled, “green” CCA for manufacturing “green” spray-foam insulation. Specifically, we wanted to discern the attitudes and overall perspectives of buyers/sellers (i.e., utilities and wood treating companies) of CCA preservatives and treated wood products, disposal methods and costs for decommissioned CCA-treated wood, and understand perceptions of and willingness-to-pay for “green” CCA preservatives extracted from the technologies used in this research. Results show that 60% of wood preservative treating respondents and 60% of electric utility company respondents are somewhat or greatly interested in using out-of-service utility poles as feedstock for “green insulation” as part of a new potential business venture.

EFFECT OF TIMBER DENSITY ON RETENTION OF WOOD PRESERVATIVES

The amount of CCA fixed in the southern pine treated with CCA have been eval-uated.The conclusions from these experiments are:with the timber density increases,the reten-tion based on weight/weight will decrease,but the retention based on weight/volume will bealmost constant.Retention by weight would be more influential than by volume.

Method Development and Validation of the Simultaneous Analysis of Methylisothiazolinone, Methylchloroisothiazolinone, Benzisothiazolinone and Bronopol in Washing-Up Liquid

A method of analysis for the simultaneous determination of methylisothiazolinone (MI), methylchloroisothiazolinone (CMI), benzisothiazolinone (BIT) and Bronopol (BNP) in washing-up liquid was established. The method consisted of a gradient HPLC analysis at three different wavelengths. The four compounds could be analyzed with good precision and accuracy.

Bacteriocins as antimicrobial and preservative agents in food:Biosynthesis,separation and application

Emerging evidence shows the trend of using safe and natural preservatives like bacteriocins in food processing.The increasing demand by the food industry to extend keeping quality and prevent spoilage of various food products has appealed for new preservatives and new methods of conservation.Further,to discover the novel spectrum of antimicrobial compounds that could effectively battle food-borne pathogens,bacteriocins have been intensively studied in the last few years.Recently,great attention has been paid to the application of lactic acid-bacterial bacteriocins targeting food spoilage-causing or pathogenic microorganisms with no significant side effects.Different mechanisms of action like pore-formation,retarding cell-wall/nucleic acid/protein synthesis have been proposed and described for diverse bacteriocins.Therefore,the syntheses,purification of bacteriocins in addition to their applications as antimicrobial and preservative agents in food processing are reviewed in detail.The review will also discuss the preservative effect of bacteriocins and their combinations having multiple modes of action(hurdle approach)to reduce the microbial load as well as antimicrobial resistance.

Effect of quercetin-containing preservatives and modified atmospheric packaging on the production of biogenic amines in Pacific white shrimp(Litopenaeus vannamei)

To investigate the productions of biogenic amines(BAs)in Pacific white shrimp treated by compound preservatives(P,0.05 g/L quercetin,0.025 g/L 4-hexylresorcinol,and 0.025 g/L cinnamic acid)in combination with modified atmosphere packaging(M,80% CO_(2)/10% O_(2)/10% N_(2)),four groups of samples treated with or without preservatives and alone or in combination with modified atmosphere packaging(A,A+P,M,M+P)during storage at 4℃for 12 days were analyzed.Most BAs increased in all samples,especially,putrescine and cadaverine.MAP inhibited the increase of most BAs(except for tyramine)and psychrotrophic bacterial counts.The inhibitory effect of the compound preservatives on bacterial growth and BAs was limited in aerobically-and MAP-stored samples,but they retarded the production of tyramine,which accumulated more rapidly in MAP.Lowest total BAs and bacterial counts were found in sample M+P(78.3 mg/kg and 5.82 log cfu/g,day 12),indicating that the combined treatment of the preservatives and MAP could reduce the risk of disorders caused by BAs.