PRiME HLB前处理技术联合超高效液相色谱-三重四极杆质谱系统检测水产品中17种磺胺类药物残留

使用通过型固相萃取小柱PRi ME HLB处理水产品样品,建立了一种水产品中17种磺胺类药物的简单、快速的筛选分析方法。水产品样品经80%乙腈水溶液(含0.2%甲酸)提取,过PRi ME HLB固相萃取柱净化,浓缩后经C_(18)色谱柱梯度洗脱分离,超高效液相色谱-三重四极杆质谱系统进行定量分析。结果表明,17种磺胺类药物在1.0~50.0 ng·mL^(-1)线性关系良好,相关系数R^(2)>0.99;该方法检出限为2μg·kg^(-1);添加浓度为10μg·kg^(-1)时方法回收率在71.3%~118.4%,RSD值均小于20%。

引导编辑技术的研究进展及应用

以CRISPR/Cas9为基础的引导编辑系统是新开发出的一种基因编辑技术,可以精确实现12种碱基的互换、插入和缺失,并且不需要产生双链断裂和引入外源供体DNA。本文从基因编辑的发展、引导编辑系统的原理、特点、优化、脱靶效应、在动植物和基因治疗研究中的应用、引导编辑系统的设计等几方面进行综述,为促进相关领域的科研工作者了解引导编辑系统及进一步利用引导编辑系统在动植物科学基础研究和育种方面的应用提供指导。

基于虚拟现实的船舶航行复杂周围环境图像三维仿真

为提升船舶在复杂航行环境中的安全,提出基于虚拟现实的船舶航行复杂周围环境图像三维仿真方法。该方法使用虚拟现实技术中的Vega Prime虚拟现实开发工具,依据海洋、船舶相关数据与图像,使用Crertor工具建立flt静态三维模型库,从该模型库内导出船舶三维模型所需数据后,使用Vega Prime虚拟现实开发工具的LynXPrime图形界面,对船舶航行复杂周围环境的水体、海浪等进行建模并进行纹理制作后,再通过实时驱动、视景仿真驱动以及仿真交互程序运行,实现船舶航行复杂周围环境图像三维仿真模型的交互。实验结果表明,该方法可有效对船舶航行复杂周围环境的洋流流向、流速、海水温度进行三维仿真。同时也可对船舶航行环境内其他船舶进行红外视景可视化,且应用效果较佳。

Relationship between Seed Priming Mediated Seedling Vigor and Yield Performance of Spring Wheat

Seed priming is a pre-germinated technique that can enhance seed germination percentage,faster and synchro-nized germination,better seedling growth,and yield under stress conditions.To ascertain the most effective seed priming method that would ensure the potential yield of wheat in Bangladesh,two experiments were carried out from December 2021 to March 2022 at the Department of Agronomy,Bangladesh Agricultural University.Two wheat varieties namely BARI Gom-28 and BWMRI Gom-1 were subjected to a range of priming chemicals in both lab and pot tests.These compounds included the following:control(no priming),hydropriming(distilled water),10000 ppm KNO_(3),15000 ppm KNO_(3),40000 ppm Mannitol,60000 ppm Mannitol,10000 ppm NaCl,20000 ppm NaCl,100 ppm PEG,150 ppm PEG,500 ppm NaOCl,1000 ppm NaOCl,10000 ppm CaCl_(2),20000 ppm CaCl_(2),10000 ppm KCl and 20000 ppm KCl.A complete randomized design(CRD)with three repli-cations was used to set up the experiments.The results showed that BARI Gom-28 and BWMRI Gom-1 responded best to KCl priming in terms of rapid seed germination and strong seedling development.On the other hand,the best priming agents for plant growth and productivity turned out to be CaCl_(2) and KCL.The results of this study support the possibility of using seed priming as a technique to improve wheat plant development and output by raising seed emergence and survival rates.

不同功能成分底涂剂对氧化锆耐久粘接强度的影响

目的比较含10-甲基丙烯酰氧癸基磷酸酯(10-metracryloyloxdecyl dyhidrogen phosphate,10-MDP)和硅烷的混合型底涂剂与MDP单一功能成分型底涂剂对氧化锆初期及耐久粘接强度的影响。方法制作氧化锆标准试件84个,随机分成4组(n=21),分别使用含10-MDP的Z-Prime Plus(ZP)、含硅烷偶联剂的Monobond-S(MS)、含MDP和硅烷偶联剂的混合型底涂剂Clearfil Ceramic Primer(CCP)、及不使用底涂剂(空白)处理试件表面,再用不含MDP的Duo-Link(DUO)树脂水门汀与氧化锆粘接,形成ZP、MS、CCP及空白对照组共4组粘接试件。每组粘接试件再随机分为3个亚组(n=7),分别进行3个时间点的老化实验,包括37℃恒温水浴24 h、30 d、30 d加3000个5~55℃冷热循环(30 d/TC)。用计算机控制万能材料实验机测定剪切粘接强度值,扫描电镜(scanning electron microscope,SEM)观察并分析断裂试样的粘接界面破坏模式,2-way ANOVA进行统计分析。结果ZP、MS、CCP三组在3个时间点获得的粘接强度值均显著高于空白对照(P<0.01);从24 h到30 d,三组粘接强度值均显著增高(P<0.05);从30 d到30 d/TC,三组粘接强度值均显著下降(P<0.01),并降至基线(24 h)以下水平(30 d/TC vs.24 h,P<0.01)。混合型底涂剂CCP在所有时间点的粘接强度值均高于单一功能成分型底涂剂ZP、MS(P<0.01)。在单一功能成分底涂剂中,ZP在30 d时的粘接强度高于MS(P=0.029),在30 d/TC时却低于MS(P=0.037)。从30 d到30 d/TC,ZP的粘接强度衰减百分率明显高于MS(82.43%vs.64.90%)。结论含MDP和硅烷的混合型底涂剂与氧化锆陶瓷的粘接强度优于单一功能成分型底涂剂。

Use of priming strategies to advance the clinical application of mesenchymal stromal/stem cell-based therapy

Mesenchymal stromal/stem cells(MSCs)have garnered significant attention in the field of regenerative medicine due to their remarkable therapeutic potential.MSCs play a pivotal role in maintaining tissue homeostasis and possess diverse functions in tissue repair and recovery in various organs.These cells are charac-terized by easy accessibility,few ethical concerns,and adaptability to in vitro cultures,making them a valuable resource for cell therapy in several clinical conditions.Over the years,it has been shown that the true therapeutic power of MSCs lies not in cell engraftment and replacement but in their ability to produce critical paracrine factors,including cytokines,growth factors,and exosomes(EXOs),which modulate the tissue microenvironment and facilitate repair and regeneration processes.Consequently,MSC-derived products,such as condi-tioned media and EXOs,are now being extensively evaluated for their potential medical applications,offering advantages over the long-term use of whole MSCs.However,the efficacy of MSC-based treatments varies in clinical trials due to both intrinsic differences resulting from the choice of diverse cell sources and non-standardized production methods.To address these concerns and to enhance MSC therapeutic potential,researchers have explored many priming strategies,including exposure to inflammatory molecules,hypoxic conditions,and three-dimensional culture techniques.These approaches have optimized MSC secretion of functional factors,empowering them with enhanced immunomodulatory,angiogenic,and regenerative properties tailored to specific medical conditions.In fact,various priming strategies show promise in the treatment of numerous diseases,from immune-related disorders to acute injuries and cancer.Currently,in order to exploit the full therapeutic potential of MSC therapy,the most important challenge is to optimize the modulation of MSCs to obtain adapted cell therapy for specific clinical disorders.In other words,to unlock the complete potential of MSCs in regenerative medicine,it is crucial to identify the most suitable tissue source and develop in vitro manipulation protocols specific to the type of disease being treated.

UPLC-MS/MS测定特殊医学用途配方乳粉中姜黄素类化合物

建立基于通过式固相萃取净化检测特殊医学用途配方乳粉中姜黄素的超高效液相色谱串联质谱检测方法。样品经80%乙腈提取,通过Oasis PRiME HLB固相萃取柱净化后,以乙腈-0.1%甲酸溶液作为流动相,在acquity UPLC HSS T_(3)色谱柱上进行梯度洗脱,经超高效液相色谱仪进行分离后,通过电喷雾正离子模式电离,多反应监测模式进行检测分析,外标法定量。对目标物进行稳定性研究,对样品中目标物提取、净化方式、色谱分离条件、质谱条件等关键因素进行了优化。结果表明,3种姜黄素类化合物在25~2 500 ng/mL范围内线性关系好,相关系数高于0.99,加标回收率在87.9%~102.8%之间,精密度在1.31%~4.94%之间,3种姜黄素类化合物的检出限为10μg/kg,定量限为25μg/kg。该方法定性定量结果准确、稳定性好、灵敏度高,能够对特殊医学用途配方乳粉中姜黄素类化合物进行定性分析与定量检测。

Origin of Sexy Prime Numbers, Origin of Cousin Prime Numbers, Equations from Supposedly Prime Numbers, Origin of the Mersenne Number, Origin of the Fermat Number

We have found through calculations that the differences between the closest supposed prime numbers other than 2 and 3 defined in the articles are: 2;4: and 6. For those whose difference is equal to 6, we showed their origin then we classified them into two categories according to their classes, we showed in which context two prime numbers which differ from 6 are called sexy and in what context they are said real sexy prime. For those whose difference is equal to 4, we showed their origin then we showed that two prime numbers which differ from 4, that is to say two cousin prime numbers, are successive. We made an observation on the supposed prime numbers then we established two pairs of equations from this observation and deduced the origin of the Mersenne number and that of the Fermat number.

How Prime Numbers Are Interconnected and Built with Two Equations: Addition and Subtraction Rules the Function (6µ)

Are all prime numbers linked by four simple functions? Can we predict when a prime will appear in a sequence of primes? If we classify primes into two groups, Group 1 for all primes that appear before ζ (such that , for instance 5, ), an even number divisible by 3 and 2, and Group 2 for all primes that are after ζ (such that , for instance 7), then we find a simple function: for each prime in each group, , where n is any natural number. If we start a sequence of primes with 5 for Group 1 and 7 for Group 2, we can attribute a μ value for each prime. The μ value can be attributed to every prime greater than 7. Thus for Group 1, and . Using this formula, all the primes appear for , where μ is any natural number.

Preliminary Identification of a Prime Number Other Than 2 and 3, the Origin of Twin Prime Numbers, the Structure of the Chain of Prime Numbers and the Set of Prime Numbers Less Than a Given Integer

The application of the Euclidean division theorem for the positive integers allowed us to establish a set which contains all the prime numbers and this set we called it set of supposedly prime numbers and we noted it Esp. We subsequently established from the previous set the set of non-prime numbers (the set of numbers belonging to this set and which are not prime) denoted Enp. We then extracted from the set of supposedly prime numbers the numbers which are not prime and the set of remaining number constitutes the set of prime numbers denoted Ep. We have deduced from the previous set, the set of prime numbers between two natural numbers. We have explained during our demonstrations the origin of the twin prime numbers and the structure of the chain of prime numbers.

On Perron’s Formula and the Prime Numbers

The Riemann hypothesis is intimately connected to the counting functions for the primes. In particular, Perron’s explicit formula relates the prime counting function to fixed points of iterations of the explicit formula with particular relations involving the trivial and non-trivial roots of the Riemann Zeta function and the Primes. The aim of the paper is to demonstrate this relation at the fixed points of iterations of explicit formula, defined by functions of the form limT∈Ν→∞fT(zw)=zw,where, zwis a real number.

The Periodic Table of Primes

Over millennia, nobody has been able to predict where prime numbers sprout or how they spread. This study establishes the Periodic Table of Primes (PTP) using four prime numbers 2, 3, 5, and 7. We identify 48 integers out of a period 2×3×5×7=210 to be the roots of all primes as well as composites without factors of 2, 3, 5, and 7. Each prime, twin primes, or composite without factors of 2, 3, 5, and 7 is an offspring of the 48 integers uniquely allocated on the PTP. Three major establishments made in the article are the Formula of Primes, the Periodic Table of Primes, and the Counting Functions of Primes and Twin Primes.

On the Number of Primes in the Interval (x, 2x) by an Elementary Method

An elementary formula to know the number of primes in the interval (x, 2x) close to the exact figure for a fixed x is given here. A new elementary equation is derived (a relation between prime numbers and composite numbers distributed in the interval [1, 2x]). An elementary method to know the number of primes in a given magnitude is suitably placed in the form of a general formula, and we have proved it. The general formula is applied to the terms of the equation, and a tactical simplification of the terms gives rise to an expression whose verification envisages scope for its further studies.

Primetime Fashion Beijing hosts China Fashion Week A/W 2024

The China Fashion Week A/W 2024,hosted by the China Fashion Designers Association,took place in Beijing from March 23 to 31.Themed“Fu”(Empower),this season’s fashion week continued to focus on Chinese aesthetics,innovation in intangible cultural heritage,fusion of Chinese trends.

A New Proof for Congruent Number’s Problem via Pythagorician Divisors

Considering Pythagorician divisors theory which leads to a new parameterization, for Pythagorician triplets ( a,b,c )∈ ℕ 3∗ , we give a new proof of the well-known problem of these particular squareless numbers n∈ ℕ ∗ , called congruent numbers, characterized by the fact that there exists a right-angled triangle with rational sides: ( A α ) 2 + ( B β ) 2 = ( C γ ) 2 , such that its area Δ= 1 2 A α B β =n;or in an equivalent way, to that of the existence of numbers U 2 , V 2 , W 2 ∈ ℚ 2∗ that are in an arithmetic progression of reason n;Problem equivalent to the existence of: ( a,b,c )∈ ℕ 3∗ prime in pairs, and f∈ ℕ ∗ , such that: ( a−b 2f ) 2 , ( c 2f ) 2 , ( a+b 2f ) 2 are in an arithmetic progression of reason n;And this problem is also equivalent to that of the existence of a non-trivial primitive integer right-angled triangle: a 2 + b 2 = c 2 , such that its area Δ= 1 2 ab=n f 2 , where f∈ ℕ ∗ , and this last equation can be written as follows, when using Pythagorician divisors: (1) Δ= 1 2 ab= 2 S−1 d e ¯ ( d+ 2 S−1 e ¯ )( d+ 2 S e ¯ )=n f 2;Where ( d, e ¯ )∈ ( 2ℕ+1 ) 2 such that gcd( d, e ¯ )=1 and S∈ ℕ ∗ , where 2 S−1 , d, e ¯ , d+ 2 S−1 e ¯ , d+ 2 S e ¯ , are pairwise prime quantities (these parameters are coming from Pythagorician divisors). When n=1 , it is the case of the famous impossible problem of the integer right-angled triangle area to be a square, solved by Fermat at his time, by his famous method of infinite descent. We propose in this article a new direct proof for the numbers n=1 (resp. n=2 ) to be non-congruent numbers, based on an particular induction method of resolution of Equation (1) (note that this method is efficient too for general case of prime numbers n=p≡a ( ( mod8 ) , gcd( a,8 )=1 ). To prove it, we use a classical proof by induction on k , that shows the non-solvability property of any of the following systems ( t=0 , corresponding to case n=1 (resp. t=1 , corresponding to case n=2 )): ( Ξ t,k ){ X 2 + 2 t ( 2 k Y ) 2 = Z 2 X 2 + 2 t+1 ( 2 k Y ) 2 = T 2 , where k∈ℕ;and solutions ( X,Y,Z,T )=( D k , E k , f k , f ′ k )∈ ( 2ℕ+1 ) 4 , are given in pairwise prime numbers.2020-Mathematics Subject Classification 11A05-11A07-11A41-11A51-11D09-11D25-11D41-11D72-11D79-11E25 .

Distribution and Relation of Primes with Tesla Numbers 3, 6, and 9

This work presents a different approach to twin primes, an approach from the perspective of the Tesla numbers and gives a refresh and new observation of twin primes that could lead us to an answer to the Twin Prime Conjecture problem. We expose a peculiar relation between twin primes and the generation of prime numbers with Tesla numbers. Tesla numbers seem to be present in so many domains like time, vibration and frequency [1], and the space between twin primes is not the exception. Let us say that twin primes are more than just prime numbers plus 2 or minus 2, and Tesla numbers are more involved with twin primes than we think, and hopefully, this approach give us a better understanding of the distribution of the twin pairs.

The Nikola Tesla Constant and Its Relation to the Circumference Inscribed in a Square

This research work relates the surface of a square and the area circumscribed by a circle, resulting in a value called Nikola Tesla constant. This constant starts with the calculation of the areas of the square and the inscribed circle, giving a ratio of 9/40 and from which a residual area of the area proportions of the geometric figures described is obtained. Plotting smooth curves, particularly those in round shapes, can be represented efficiently with the use of Nikola Tesla constant, reducing complex mathematical calculus.

安德里茨向永丰余提供的生活用纸生产线成功开机

由安德里茨供货的全新PrimeLineCOMPACT M1600生活用纸生产线在中国台湾永丰余消费品实业股份有限公司清水基地成功开机。安德里茨提供的这台PrimeLineCOMPACT卫生纸机,幅宽为3.65m,设计车速可达1,650m/min,用于生产卫生纸、餐巾纸、手帕纸和面巾纸。该纸机配备了一系列的创新技术,包括:PrimeFlow双层流浆箱,可减少唇口变形,获得最佳的横幅布浆品质;新型的PrimePress XT Evo靴压技术和Impulse靴套,确保具有优良松厚度和高压榨后干度的高品质纸张;PrimeDry钢制扬克缸(18ft)及Canopy Hood通风气罩,实现高效干燥的同时显著节约蒸汽。

安德里茨供货的TM27、TM28卫生纸机在恒安集团成功开机

近日,两台由安德里茨供货的PrimeLine紧凑型卫生纸机在广东恒安纸业云浮工厂顺利开机。至此,共有15台安德里茨卫生纸机在恒安纸业运行,总产能达到78万t/a。这两台PrimeLineCOMPACT M 1600纸机的设计车速为1,700m/min,幅宽为3.65m,采用原生木浆为原料,生产面巾纸、卫生纸、手帕纸和餐巾纸。

安德里茨将向中顺洁柔宿迁基地提供两台Prime LineCOMPACT M紧凑型卫生纸机

安德里茨将向中顺洁柔位于江苏省宿迁市宿城区运河宿迁港产业园的生活用纸生产基地提供两台Prime LineCOMPACT M紧凑型卫生纸机。这两台纸机的最大运行车速可达1,650m/min,幅宽3.5m,以原生商品浆为原料,最大产能为94t/d,用以生产生活用纸原纸。两台纸机均配备了安德里茨先进的Prime系列产品,包括Prime Flow流浆箱,Prime Dry钢制扬克缸和Prime Dry Canopy通风式气罩等,并采用大直径高负荷真空压榨辊,确保在生产高品质纸品同时通过高效干燥方式实现低能耗生产。