AIM:To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.sothelin expression in INS-1 cells was carried out to investigate the effect of mesothelin during cell prolifera...AIM:To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.sothelin expression in INS-1 cells was carried out to investigate the effect of mesothelin during cell proliferation and cell apoptosis in vitro.Adenovirus-mediated RNA interference was performed to block mesothelin in vivo to directly assess the role of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.RESULTS:Exogenous overexpression of mesothelin promoted cell proliferation,cell colony formation and enhanced cell resistance to apoptosis of INS-1 cells.Down-regulation of mesothelin made no difference in cell proliferation and apoptosis compared with that in the control group.After an injection of adenovirusmesothelin,a significantly increased number of small islets appeared,and the expression of PCNA was decreased on day 7 and day 14 compared with the AdEGFP group.CONCLUSION:Mesothelin was able to promoteβcell proliferation in the remodeling stage of neonatal rats.Mesothelin may have an important role in the remodeling of the endocrine pancreas in neonatal rats.展开更多
基金Supported by National Natural Science Foundation of China,No.81070620 and No.81170715Jiangsu Province’s Universities Natural Science Research,No.09KJB320009
文摘AIM:To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.sothelin expression in INS-1 cells was carried out to investigate the effect of mesothelin during cell proliferation and cell apoptosis in vitro.Adenovirus-mediated RNA interference was performed to block mesothelin in vivo to directly assess the role of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.RESULTS:Exogenous overexpression of mesothelin promoted cell proliferation,cell colony formation and enhanced cell resistance to apoptosis of INS-1 cells.Down-regulation of mesothelin made no difference in cell proliferation and apoptosis compared with that in the control group.After an injection of adenovirusmesothelin,a significantly increased number of small islets appeared,and the expression of PCNA was decreased on day 7 and day 14 compared with the AdEGFP group.CONCLUSION:Mesothelin was able to promoteβcell proliferation in the remodeling stage of neonatal rats.Mesothelin may have an important role in the remodeling of the endocrine pancreas in neonatal rats.
