Many students often raise questions, "Why it is so difficult to improve the listening comprehension?" This cannot be solved easily, because listening is a kind of complex and perceptual knowledge process. Li...Many students often raise questions, "Why it is so difficult to improve the listening comprehension?" This cannot be solved easily, because listening is a kind of complex and perceptual knowledge process. Listening does not only mean the ability to hear, it is also the ability to capture the information that the language carries through hearing organs and the ability of head. As far as I am concerned, such a problem is related to the complexity of various factors: listening itself covers, the language knowledge proficiency listeners possess, and also it is closely connected with the traditional foreign language style.展开更多
As the chief architect of China’s reform and opening up and a leading personalityof world class,Deng Xiaoping played a historical role in China’s political and e-conomic development in the period of its reform and o...As the chief architect of China’s reform and opening up and a leading personalityof world class,Deng Xiaoping played a historical role in China’s political and e-conomic development in the period of its reform and opening.And so did his globalstrategic thinking,which not only had a decisive bearing on the adjustments,andchanges in China’s foreign strategy and policies,but also made a great展开更多
Anna was a vivid literature figure in the international literature history. Besides the obvious social factors of Anna 's tragedy, some internal roots of her tragedy shouldn't be neglected, such as her particu...Anna was a vivid literature figure in the international literature history. Besides the obvious social factors of Anna 's tragedy, some internal roots of her tragedy shouldn't be neglected, such as her particular spiritual world, merit and drawback of her character, her love value and conflicts about her thought. Analyzing into internal factors for Anna 's tragedy would offer further appreciation to readers.展开更多
—"Where do I begin?"The place to begin is with your own assumptions about what students should learn and how they learn best.Curriculum plans are most likely to be acted on when their assumptions coincide w...—"Where do I begin?"The place to begin is with your own assumptions about what students should learn and how they learn best.Curriculum plans are most likely to be acted on when their assumptions coincide with the teacher’s ideas about learning.When teachers are required to use plans whose assumptions differ from their own,they adapt them to suit their own purposes.展开更多
Soft condensed-state physics is a disciplinary frontier of 20th-century physics. An interdiscipline in nature, it involves biology, chemistry and even pure mathematics. Taking the liquid crystal (LC) biomembrane as an...Soft condensed-state physics is a disciplinary frontier of 20th-century physics. An interdiscipline in nature, it involves biology, chemistry and even pure mathematics. Taking the liquid crystal (LC) biomembrane as an example, this article expounds the current development trend of this new and promising branch of contemporary physics.展开更多
At the present time, mass me- dia and new media play an important part in establish- ing and constructing a civil society. This paper will focus on educa- tional roles of the media and will probe the major experiences...At the present time, mass me- dia and new media play an important part in establish- ing and constructing a civil society. This paper will focus on educa- tional roles of the media and will probe the major experiences and challenges related to constructing news events with human rights as the framework.展开更多
Traditionally, literary reading is regarded as a practicalmeans to stimulate the interest of EFL students in their study ofEnglish to accelerate the development of their L2 acquisition;literary works are considered a ...Traditionally, literary reading is regarded as a practicalmeans to stimulate the interest of EFL students in their study ofEnglish to accelerate the development of their L2 acquisition;literary works are considered a prime resource of linguistic inputto heighten the quality of learners’ target language. Yet, the re-search findings from this study indicate that such linguistic inputcannot effectively benefit the English majors when their L2 is notwell developed to cope efficaciously with such readers. In addi-tion, research findings also indicate that many literary wordsand phrases related to another cultural and social background notonly intensify the obstacles to proper comprehension but alsolimit opportunity for practical application of this sort of linguis-tic input in the social milieu where the learners live. Therefore,how to cope with literary works in language learning and whatsort of linguistic input that can effectively stimulate the develop-ment of learners’ L2 acquisition in their own social environmentneed to be explored.展开更多
During the 20 years of after opening and reform, with the pro-gressive establishment of the new institutional frame of socialist mar-ket economy, China national econo-my has registered a continuous in-crease in market...During the 20 years of after opening and reform, with the pro-gressive establishment of the new institutional frame of socialist mar-ket economy, China national econo-my has registered a continuous in-crease in marketalization and social-ization level. By the end of 1998,展开更多
Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluor...Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluorescence of terbium ions(Tb^(3+))via binding with single-strand DNA.Mercury ion,Hg^(2+)induced thymine(T)-rich DNA strand to form a double-strand structure(T-Hg^(2+)-T),thus leading to fluorescence reduction.Based on the principle,Hg^(2+)can be quantified based on the fluorescence of Tb^(3+),the limit of detection was 0.0689μmol/L and the linear range was 0.1-6.0μmol/L.Due to the specificity of T-Hg^(2+)-T artificial base pair,the assay could distinguish Hg^(2+)from other metal ions.The recovery rate was ranged in 98.71%-101.34%for detecting mercury pollution in three food samples.The assay is low-cost,separation-free and mix-to-read,thus was a competitive tool for detection of mercury pollution to ensure food safety.展开更多
Curcumin and its derivatives have good electrical and optical properties due to the highly symmetric structure of delocalized π electrons. Apart from that, curcumin and its derivatives can interact with numerous mole...Curcumin and its derivatives have good electrical and optical properties due to the highly symmetric structure of delocalized π electrons. Apart from that, curcumin and its derivatives can interact with numerous molecular targets, thereby exerting less side effects on human body. The fluorescence emission wavelength and fluorescence intensity of curcumin can be enhanced by modifying its π-conjugated system and β-diketone structure. Some curcumin-based fluorescent probes have been utilized to detect soluble/insoluble amyloid-β protein, intracranial reactive oxygen species, cysteine, cancer cells, etc. Based on the binding characteristics of curcumin-based fluorescent probes with various target molecules, the factors affecting the fluorescence intensity and emission wavelength of the probes are analyzed, in order to obtain a curcumin probe with higher sensitivity and selectivity. Such an approach will be greatly applicable to in vivo fluorescence imaging.展开更多
Cytosolic Ca^(2+)plays a key role in signal transduction in plants.Calcium imaging is the most common approach to studying dynamic changes in the cytoplasmic Ca^(2+)content.Here,we used mature‘Fuji’apples(Malus pumi...Cytosolic Ca^(2+)plays a key role in signal transduction in plants.Calcium imaging is the most common approach to studying dynamic changes in the cytoplasmic Ca^(2+)content.Here,we used mature‘Fuji’apples(Malus pumila Mill.)to obtain viable protoplasts from flesh tissue cells by enzymatic hydrolysis;then,three small-molecule fluorescent probes(fluo-8/AM,fluo-4/AM,and rhod-2/AM)were loaded into the protoplasts.All three Ca^(2+)fluorescent probes successfully entered the cytoplasm but did not enter the vacuole.Both the Ca^(2+)chelator(EGTA)and Ca^(2+)channel blocker(La3+)reduced the fluorescence intensity in the cytoplasm.The calcium ionophore A23187 increased the fluorescence intensity in the cytoplasm at 5μmol/L but decreased it at 50μmol/L.Additionally,A23187 reversed the fluorescence intensity in the cytoplasm,which was decreased by La3+.Ionomycin is also a calcium ionophore that can increase the fluorescence intensity of calcium in the cytoplasm.These results suggest that small-molecule Ca^(2+)fluorescent probes can be used to detect changes in cytosolic calcium levels in the cells of fruit flesh tissue.In addition,the optimum concentration of fluo-8/AM was determined to be 5μmol/L.This was the first time that protoplasts have been isolated from apple flesh tissue cells and small-molecule fluorescent probes have been used to detect calcium in the cytoplasm of flesh tissue cells.This study provides a new method to study calcium signal transduction in fruit flesh tissue.展开更多
A flexible or planar eddy current probe with a differential structure can suppress the lift-off noise during the inspection of defects.However,the extent of the lift-off effect on differential probes,including differe...A flexible or planar eddy current probe with a differential structure can suppress the lift-off noise during the inspection of defects.However,the extent of the lift-off effect on differential probes,including different coil structures,varies.In this study,two planar eddy current probes with differential pickup structures and the same size,Koch and circular probes,were used to compare lift-off effects.The eddy current distributions of the probes perturbed by 0°and 90°cracks were obtained by finite element analysis.The analysis results show that the 90°crack can impede the eddy current induced by the Koch probe even further at relatively low lift-off distance.The peak-to-peak values of the signal output from the two probes were compared at different lift-off distances using finite element analysis and experimental methods.In addition,the effects of different frequencies on the lift-off were studied experimentally.The results show that the signal peak-to-peak value of the Koch probe for the inspection of cracks in 90°orientation is larger than that of the circular probe when the lift-off distance is smaller than 1.2 mm.In addition,the influence of the lift-off distance on the peak-to-peak signal value of the two probes was studied via normalization.This indicates that the influence becomes more evident with an increase in excitation frequency.This research discloses the lift-off effect of differential planar eddy current probes with different coil shapes and proves the detection merit of the Koch probe for 90°cracks at low lift-off distances.展开更多
Rheumatoid arthritis(RA)is a systemic autoimmune disease that is primarily manifested as synovitis and polyarticular opacity and typically leads to serious joint damage and irreversible disability,thus adversely affec...Rheumatoid arthritis(RA)is a systemic autoimmune disease that is primarily manifested as synovitis and polyarticular opacity and typically leads to serious joint damage and irreversible disability,thus adversely affecting locomotion ability and life quality.Consequently,good prognosis heavily relies on the early diagnosis and effective therapeutic monitoring of RA.Activatable fluorescent probes play vital roles in the detection and imaging of biomarkers for disease diagnosis and in vivo imaging.Herein,we review the fluorescent probes developed for the detection and imaging of RA biomarkers,namely reactive oxygen/nitrogen species(hypochlorous acid,peroxynitrite,hydroxyl radical,nitroxyl),pH,and cysteine,and address the related challenges and prospects to inspire the design of novel fluorescent probes and the improvement of their performance in RA studies.展开更多
Special appearanceAs an ancient town in the south of the Yangtze River, Zhujiajiao has its own peculiar appearance differing from its counterparts. Its feature is obvious with the town encircled by water and the urban...Special appearanceAs an ancient town in the south of the Yangtze River, Zhujiajiao has its own peculiar appearance differing from its counterparts. Its feature is obvious with the town encircled by water and the urban area crisscrossed by rivers. The downtown area is linked by lanes, bridges, riverside comdors and courtyards.展开更多
Feline calicivirus(FCV)was a highly prevalent RNA virus causing upper respiratory tract infections in cats through the mouth and nose around the world,result in the death of cats especially under 1 year old with upper...Feline calicivirus(FCV)was a highly prevalent RNA virus causing upper respiratory tract infections in cats through the mouth and nose around the world,result in the death of cats especially under 1 year old with upper respiratory tract disease(URTD)and virulent systemic disease(FCV-VSD)[1].Infected cats usually presented with mouth ulcers,salivation and gingivitis-stomatitis.Furthermore,both recovered cats and asymptomatic carriers can still carry and spread the FCV virus for extended periods of time,contributing to the potential for outbreak.This presented a significant threat to the health and lives of cats,and rare wildlife and took a heavy financial loss on the pet industry,making the prevention and control of the virus difficult.Hence,rapid identification of FCV in clinical samples was necessary to prevent the spread of FCV and avoid financial loss.展开更多
Uniaxial strain is a powerful tuning parameter that can control symmetry and anisotropic electronic properties in iron-based superconductors.However,accurately characterizing anisotropic strain can be challenging and ...Uniaxial strain is a powerful tuning parameter that can control symmetry and anisotropic electronic properties in iron-based superconductors.However,accurately characterizing anisotropic strain can be challenging and complex.Here,we utilize a cryogenic optical system equipped with a high-spatial-resolution microscope to characterize surface strains in iron-based superconductors using the digital image correlation method.Compared with other methods such as high-resolution x-ray diffraction,strain gauge,and capacitive sensor,digital image correlation offers a non-contact full-field measurement approach,acting as an optical virtual strain gauge that provides high spatial resolution.The results measured on detwinned BaFe_(2)As_(2)are quantitatively consistent with the distortion measured by x-ray diffraction and neutron Larmor diffraction.These findings highlight the potential of cryogenic digital image correlation as an effective and accessible tool for probing the isotropic and anisotropic strains,facilitating applications of uniaxial strain tuning in research of quantum materials.展开更多
文摘Many students often raise questions, "Why it is so difficult to improve the listening comprehension?" This cannot be solved easily, because listening is a kind of complex and perceptual knowledge process. Listening does not only mean the ability to hear, it is also the ability to capture the information that the language carries through hearing organs and the ability of head. As far as I am concerned, such a problem is related to the complexity of various factors: listening itself covers, the language knowledge proficiency listeners possess, and also it is closely connected with the traditional foreign language style.
文摘As the chief architect of China’s reform and opening up and a leading personalityof world class,Deng Xiaoping played a historical role in China’s political and e-conomic development in the period of its reform and opening.And so did his globalstrategic thinking,which not only had a decisive bearing on the adjustments,andchanges in China’s foreign strategy and policies,but also made a great
文摘Anna was a vivid literature figure in the international literature history. Besides the obvious social factors of Anna 's tragedy, some internal roots of her tragedy shouldn't be neglected, such as her particular spiritual world, merit and drawback of her character, her love value and conflicts about her thought. Analyzing into internal factors for Anna 's tragedy would offer further appreciation to readers.
文摘—"Where do I begin?"The place to begin is with your own assumptions about what students should learn and how they learn best.Curriculum plans are most likely to be acted on when their assumptions coincide with the teacher’s ideas about learning.When teachers are required to use plans whose assumptions differ from their own,they adapt them to suit their own purposes.
文摘Soft condensed-state physics is a disciplinary frontier of 20th-century physics. An interdiscipline in nature, it involves biology, chemistry and even pure mathematics. Taking the liquid crystal (LC) biomembrane as an example, this article expounds the current development trend of this new and promising branch of contemporary physics.
文摘At the present time, mass me- dia and new media play an important part in establish- ing and constructing a civil society. This paper will focus on educa- tional roles of the media and will probe the major experiences and challenges related to constructing news events with human rights as the framework.
文摘Traditionally, literary reading is regarded as a practicalmeans to stimulate the interest of EFL students in their study ofEnglish to accelerate the development of their L2 acquisition;literary works are considered a prime resource of linguistic inputto heighten the quality of learners’ target language. Yet, the re-search findings from this study indicate that such linguistic inputcannot effectively benefit the English majors when their L2 is notwell developed to cope efficaciously with such readers. In addi-tion, research findings also indicate that many literary wordsand phrases related to another cultural and social background notonly intensify the obstacles to proper comprehension but alsolimit opportunity for practical application of this sort of linguis-tic input in the social milieu where the learners live. Therefore,how to cope with literary works in language learning and whatsort of linguistic input that can effectively stimulate the develop-ment of learners’ L2 acquisition in their own social environmentneed to be explored.
文摘During the 20 years of after opening and reform, with the pro-gressive establishment of the new institutional frame of socialist mar-ket economy, China national econo-my has registered a continuous in-crease in marketalization and social-ization level. By the end of 1998,
基金financially supported by National Natural Science Foundation of China(22074100)the Young Elite Scientist Sponsorship Program by CAST(YESS20200036)+3 种基金the Researchers Supporting Project Number RSP-2021/138King Saud University,Riyadh,Saudi ArabiaTechnological Innovation R&D Project of Chengdu City(2019-YF05-31702266-SN)Sichuan University-Panzhihua City joint Project(2020CDPZH-5)。
文摘Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluorescence of terbium ions(Tb^(3+))via binding with single-strand DNA.Mercury ion,Hg^(2+)induced thymine(T)-rich DNA strand to form a double-strand structure(T-Hg^(2+)-T),thus leading to fluorescence reduction.Based on the principle,Hg^(2+)can be quantified based on the fluorescence of Tb^(3+),the limit of detection was 0.0689μmol/L and the linear range was 0.1-6.0μmol/L.Due to the specificity of T-Hg^(2+)-T artificial base pair,the assay could distinguish Hg^(2+)from other metal ions.The recovery rate was ranged in 98.71%-101.34%for detecting mercury pollution in three food samples.The assay is low-cost,separation-free and mix-to-read,thus was a competitive tool for detection of mercury pollution to ensure food safety.
基金financially supported by the Scientific Research Fund of Liaoning Provincial Education Department of China (No. LJC201908)the Natural Science Foundation of Liaoning Province (No.20180510016,2019-MS-153)。
文摘Curcumin and its derivatives have good electrical and optical properties due to the highly symmetric structure of delocalized π electrons. Apart from that, curcumin and its derivatives can interact with numerous molecular targets, thereby exerting less side effects on human body. The fluorescence emission wavelength and fluorescence intensity of curcumin can be enhanced by modifying its π-conjugated system and β-diketone structure. Some curcumin-based fluorescent probes have been utilized to detect soluble/insoluble amyloid-β protein, intracranial reactive oxygen species, cysteine, cancer cells, etc. Based on the binding characteristics of curcumin-based fluorescent probes with various target molecules, the factors affecting the fluorescence intensity and emission wavelength of the probes are analyzed, in order to obtain a curcumin probe with higher sensitivity and selectivity. Such an approach will be greatly applicable to in vivo fluorescence imaging.
基金supported by the National Key Research and Development Plan Project:Integrated research and demonstration on the technology of reducing application and increasing efficiency of chemical fertilizer and pesticide in apple cultivation(2016YFD0201120).
文摘Cytosolic Ca^(2+)plays a key role in signal transduction in plants.Calcium imaging is the most common approach to studying dynamic changes in the cytoplasmic Ca^(2+)content.Here,we used mature‘Fuji’apples(Malus pumila Mill.)to obtain viable protoplasts from flesh tissue cells by enzymatic hydrolysis;then,three small-molecule fluorescent probes(fluo-8/AM,fluo-4/AM,and rhod-2/AM)were loaded into the protoplasts.All three Ca^(2+)fluorescent probes successfully entered the cytoplasm but did not enter the vacuole.Both the Ca^(2+)chelator(EGTA)and Ca^(2+)channel blocker(La3+)reduced the fluorescence intensity in the cytoplasm.The calcium ionophore A23187 increased the fluorescence intensity in the cytoplasm at 5μmol/L but decreased it at 50μmol/L.Additionally,A23187 reversed the fluorescence intensity in the cytoplasm,which was decreased by La3+.Ionomycin is also a calcium ionophore that can increase the fluorescence intensity of calcium in the cytoplasm.These results suggest that small-molecule Ca^(2+)fluorescent probes can be used to detect changes in cytosolic calcium levels in the cells of fruit flesh tissue.In addition,the optimum concentration of fluo-8/AM was determined to be 5μmol/L.This was the first time that protoplasts have been isolated from apple flesh tissue cells and small-molecule fluorescent probes have been used to detect calcium in the cytoplasm of flesh tissue cells.This study provides a new method to study calcium signal transduction in fruit flesh tissue.
基金Supported by Gansu Provincial Natural Science Foundation of China(Grant No.22JR5RA229)National Natural Science Foundation of China(Grant Nos.51807086,12162021)Hongliu Youth Found of Lanzhou University of Technology and Gansu Provincial Outstanding Graduate Student Innovation Star of China(Grant No.2021CXZX-453).
文摘A flexible or planar eddy current probe with a differential structure can suppress the lift-off noise during the inspection of defects.However,the extent of the lift-off effect on differential probes,including different coil structures,varies.In this study,two planar eddy current probes with differential pickup structures and the same size,Koch and circular probes,were used to compare lift-off effects.The eddy current distributions of the probes perturbed by 0°and 90°cracks were obtained by finite element analysis.The analysis results show that the 90°crack can impede the eddy current induced by the Koch probe even further at relatively low lift-off distance.The peak-to-peak values of the signal output from the two probes were compared at different lift-off distances using finite element analysis and experimental methods.In addition,the effects of different frequencies on the lift-off were studied experimentally.The results show that the signal peak-to-peak value of the Koch probe for the inspection of cracks in 90°orientation is larger than that of the circular probe when the lift-off distance is smaller than 1.2 mm.In addition,the influence of the lift-off distance on the peak-to-peak signal value of the two probes was studied via normalization.This indicates that the influence becomes more evident with an increase in excitation frequency.This research discloses the lift-off effect of differential planar eddy current probes with different coil shapes and proves the detection merit of the Koch probe for 90°cracks at low lift-off distances.
基金supported by the National Natural Science Foundation of China(82072432)the China-Japan Friendship Hospital Horizontal Project/Spontaneous Research Funding(2022-HX-JC-7)+1 种基金the National High Level Hospital Clinical Research Funding(2022-NHLHCRF-PY-20)the Elite Medical Professionals project of China-Japan Friendship Hospital(ZRJY2021-GG12).
文摘Rheumatoid arthritis(RA)is a systemic autoimmune disease that is primarily manifested as synovitis and polyarticular opacity and typically leads to serious joint damage and irreversible disability,thus adversely affecting locomotion ability and life quality.Consequently,good prognosis heavily relies on the early diagnosis and effective therapeutic monitoring of RA.Activatable fluorescent probes play vital roles in the detection and imaging of biomarkers for disease diagnosis and in vivo imaging.Herein,we review the fluorescent probes developed for the detection and imaging of RA biomarkers,namely reactive oxygen/nitrogen species(hypochlorous acid,peroxynitrite,hydroxyl radical,nitroxyl),pH,and cysteine,and address the related challenges and prospects to inspire the design of novel fluorescent probes and the improvement of their performance in RA studies.
文摘Special appearanceAs an ancient town in the south of the Yangtze River, Zhujiajiao has its own peculiar appearance differing from its counterparts. Its feature is obvious with the town encircled by water and the urban area crisscrossed by rivers. The downtown area is linked by lanes, bridges, riverside comdors and courtyards.
基金supported by the natural science foundation reserve project of Jiangsu Agri-animal Husbandry Vocational College(No.NSF2023CB14).
文摘Feline calicivirus(FCV)was a highly prevalent RNA virus causing upper respiratory tract infections in cats through the mouth and nose around the world,result in the death of cats especially under 1 year old with upper respiratory tract disease(URTD)and virulent systemic disease(FCV-VSD)[1].Infected cats usually presented with mouth ulcers,salivation and gingivitis-stomatitis.Furthermore,both recovered cats and asymptomatic carriers can still carry and spread the FCV virus for extended periods of time,contributing to the potential for outbreak.This presented a significant threat to the health and lives of cats,and rare wildlife and took a heavy financial loss on the pet industry,making the prevention and control of the virus difficult.Hence,rapid identification of FCV in clinical samples was necessary to prevent the spread of FCV and avoid financial loss.
基金supported by the National Key Research and Development Program of China(Grant No.2021YFA1400400)the Fundamental Research Funds for the Central Universities,and the National Natural Science Foundation of China(Grant Nos.12174029 and 11922402).
文摘Uniaxial strain is a powerful tuning parameter that can control symmetry and anisotropic electronic properties in iron-based superconductors.However,accurately characterizing anisotropic strain can be challenging and complex.Here,we utilize a cryogenic optical system equipped with a high-spatial-resolution microscope to characterize surface strains in iron-based superconductors using the digital image correlation method.Compared with other methods such as high-resolution x-ray diffraction,strain gauge,and capacitive sensor,digital image correlation offers a non-contact full-field measurement approach,acting as an optical virtual strain gauge that provides high spatial resolution.The results measured on detwinned BaFe_(2)As_(2)are quantitatively consistent with the distortion measured by x-ray diffraction and neutron Larmor diffraction.These findings highlight the potential of cryogenic digital image correlation as an effective and accessible tool for probing the isotropic and anisotropic strains,facilitating applications of uniaxial strain tuning in research of quantum materials.