Conventional Langmuir probe techniques usually face the difficulty of being used in processing plasmas where dielectric compounds form,due to rapid failure by surface insulation.A solution to the problem,the so-called...Conventional Langmuir probe techniques usually face the difficulty of being used in processing plasmas where dielectric compounds form,due to rapid failure by surface insulation.A solution to the problem,the so-called harmonic probe technique,had been proposed and shown effectiveness.In this study,the technique was investigated in detail by changing bias signal amplitudes V_0,and evaluated its accuracy by comparing with the conventional Langmuir probe.It was found that the measured electron temperature Teincreased with V_0,but showing a relatively stable region when V_0〉Te/e in which it was close to the true Tevalue.This is contrary to the general consideration that V_0should be smaller than Te/e for accurate measurement of Te.The phenomenon is interpreted by the non-negligible change of the ion current with V_0at low V_0values.On the other hand,the measured nialso increased with V_0due to the sheath expansion,and to improve the accuracy of niit needs to linearly extrapolate the ni-V_0trend to V_0=0.The results were applied to a diagnosis of the plasmas for chemical vapor deposition of diamond-like carbon thin films and the relationship between plasma parameters and films deposition rates was obtained.展开更多
A commercially available non—radioactive DNA labelling kit "enhanced chemiluminescence"(ECL) was evaluated for the diagnosis of falciparum malaria. The results showed that ECL labeled probe successfully det...A commercially available non—radioactive DNA labelling kit "enhanced chemiluminescence"(ECL) was evaluated for the diagnosis of falciparum malaria. The results showed that ECL labeled probe successfully detected as little as 25 pg. Purified DNA of 0.001％ parasitemia of cultured Plasmodium falciparum and did not react with human展开更多
In this study,a natural cotton thread immunoassay device combined with gold nanorod（GNR） reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung ca...In this study,a natural cotton thread immunoassay device combined with gold nanorod（GNR） reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung cancer related biomarker.Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device.An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle（GNP） report probe.The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry（ASV） testing,which meet the requirement for clinical diagnosis.展开更多
文摘Conventional Langmuir probe techniques usually face the difficulty of being used in processing plasmas where dielectric compounds form,due to rapid failure by surface insulation.A solution to the problem,the so-called harmonic probe technique,had been proposed and shown effectiveness.In this study,the technique was investigated in detail by changing bias signal amplitudes V_0,and evaluated its accuracy by comparing with the conventional Langmuir probe.It was found that the measured electron temperature Teincreased with V_0,but showing a relatively stable region when V_0〉Te/e in which it was close to the true Tevalue.This is contrary to the general consideration that V_0should be smaller than Te/e for accurate measurement of Te.The phenomenon is interpreted by the non-negligible change of the ion current with V_0at low V_0values.On the other hand,the measured nialso increased with V_0due to the sheath expansion,and to improve the accuracy of niit needs to linearly extrapolate the ni-V_0trend to V_0=0.The results were applied to a diagnosis of the plasmas for chemical vapor deposition of diamond-like carbon thin films and the relationship between plasma parameters and films deposition rates was obtained.
文摘A commercially available non—radioactive DNA labelling kit "enhanced chemiluminescence"(ECL) was evaluated for the diagnosis of falciparum malaria. The results showed that ECL labeled probe successfully detected as little as 25 pg. Purified DNA of 0.001％ parasitemia of cultured Plasmodium falciparum and did not react with human
基金financially supported by the National Natural Science Foundation of China (No. 21205094)NFFTBS (Nos. J1103311,J1210057)the New Faculty Startup Funds of Northwest University in Shaanxi Province (No. PR12011)
文摘In this study,a natural cotton thread immunoassay device combined with gold nanorod（GNR） reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung cancer related biomarker.Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device.An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle（GNP） report probe.The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry（ASV） testing,which meet the requirement for clinical diagnosis.
