Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens...Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore.the underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(CFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.展开更多
In order to meet the high precision requirement of wide steel strip in industry field, a novel online measurement of roller profile based on sonic circulation and pulse-echo technology was introduced. All kinds of the...In order to meet the high precision requirement of wide steel strip in industry field, a novel online measurement of roller profile based on sonic circulation and pulse-echo technology was introduced. All kinds of the factors influencing the accuracy of roller profile online measurement were analyzed in detail and error compensation analysis of system was accordingly presented. In order to reduce count error, field program gate array(FPGA) was introduced and a highprecision data acquisition system was designed based on digital phase-shift technology. Experiments indicate that the standard deviation of measure data was 7.27 μm, which showed the feasibility and validity of the proposed method, and realized the roll profile measurement with high precision.展开更多
The term ‘profile machining’ is used to refer to the milling of vertical surfaces described by profile curves. Profile machining requires higher precision (1/1000 mm) than regular 3D machining (1/100 mm) with the er...The term ‘profile machining’ is used to refer to the milling of vertical surfaces described by profile curves. Profile machining requires higher precision (1/1000 mm) than regular 3D machining (1/100 mm) with the erosion of sharp vertices should being especially avoided. Although, profile machining is very essential for making trimming and flange dies, it seldom brought into focus. This paper addresses the technological requirements of profile machining including machining width and depth control, minimizing toolware, and protecting sharp vertices. Issues of controller alarms are also addressed.展开更多
Existing pressure drilling technologies are based on different principles and display distinct characteristics in terms of control pressure and degree of formation adaptability.In the present study,the constant-bottom...Existing pressure drilling technologies are based on different principles and display distinct characteristics in terms of control pressure and degree of formation adaptability.In the present study,the constant-bottomhole-pressure(CBHP)and controlled-mud-level(CML)dual gradient drilling methods are considered.Models for the equivalent circulating density(ECD)are introduced for both drilling methods,taking into account the control pressure parameters(wellhead back pressure,displacement,mud level,etc.)and the relationship between the equivalent circulating density curve in the wellbore and two different types of pressure profiles in deep-water areas.The findings suggest that the main pressure control parameter for CBHP drilling is the wellhead back pressure,while for CML dual gradient drilling,it is the mud level.Two examples are considered(wells S1 and B2).For S1,CML dual gradient drilling only needs to adjust the ECD curve once to drill down to the target layer without risk.By comparison,CBHP drilling requires multiple adjustments to reach the target well depth avoiding a kick risk.In well B2,the CBHP method can drill down to the desired zone or even deeper after a single adjustment of the ECD curve.In contrast,CML dual-gradient drilling requires multiple adjustments to reach the target well depth(otherwise there is a risk of lost circulation).Therefore,CML dual-gradient drilling should be considered as a better choice for well S1,while CBHP drilling is more suitable for well B2.展开更多
为研究辊形电磁调控技术(roll profile electromagnetic control technology,RPECT)的热力胀形行为,采用有限元方法分析了不同电流密度及频率下综合辊凸度、热力贡献辊凸度以及力凸度占比等随接触区热量的变化规律,探究了接触区热量与...为研究辊形电磁调控技术(roll profile electromagnetic control technology,RPECT)的热力胀形行为,采用有限元方法分析了不同电流密度及频率下综合辊凸度、热力贡献辊凸度以及力凸度占比等随接触区热量的变化规律,探究了接触区热量与热力胀形能力的映射关系及其成立条件.结果表明,在接触区热量的中低取值范围内,同一接触区热量所对应的热力调控能力不会受磁参数影响,故提出将接触区热量作为输入参数与调控能力之间桥梁的RPECT高效控制策略.该策略通过提升磁参数来快速达到接触区的目标温升效果,进而获取目标调控能力.以20μm的辊缝凸度需求为例,经原始与高效控制策略间的对比发现,新策略的调控时间可有效地缩短至原调控时间的19.57%.展开更多
The most common and abundant DNA modification is 5-methylcytosine（5mC）,which has been well-established as an epigenetic mark regulating gene expression in eukaryotes（Jones,2012）.Another DNA modification N^6-methyl...The most common and abundant DNA modification is 5-methylcytosine（5mC）,which has been well-established as an epigenetic mark regulating gene expression in eukaryotes（Jones,2012）.Another DNA modification N^6-methyldeoxyadenosine（6mA）,previously reported as a widespread DNA methylation in prokaryotes.展开更多
In this report we have evaluated metabolite and RNA profiling technologies to begin to understand the natural variation in these biomolecules found in commercial-quality, conventional (non-GM) maize hybrids. Our analy...In this report we have evaluated metabolite and RNA profiling technologies to begin to understand the natural variation in these biomolecules found in commercial-quality, conventional (non-GM) maize hybrids. Our analyses focus on mature grain, the article of commerce that is most typically subjected to the rigorous studies involved in the comparative safety assessment of GM products. We have used a population of conventionally-bred maize hybrids that derive from closely related inbred parents grown under standard field conditions across geographically similar locations. This study highlights the large amount of natural variation in metabolites and transcripts across conventional maize germplasm grown under normal field conditions, and underscores the critical need for further extensive studies before these technologies can be seriously considered for utility in the comparative safety assessment of GM crops.展开更多
基金supported by Brain Korea (BK)21 Plus Project (4299990913942)funded by the Korean Government,Koreathe Collabo Project funded by the Ministry of SMEs and Startups (C1016120-01-02)the National Research Foundation of Korea (NRF) (2018007551)。
文摘Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore.the underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(CFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.
文摘In order to meet the high precision requirement of wide steel strip in industry field, a novel online measurement of roller profile based on sonic circulation and pulse-echo technology was introduced. All kinds of the factors influencing the accuracy of roller profile online measurement were analyzed in detail and error compensation analysis of system was accordingly presented. In order to reduce count error, field program gate array(FPGA) was introduced and a highprecision data acquisition system was designed based on digital phase-shift technology. Experiments indicate that the standard deviation of measure data was 7.27 μm, which showed the feasibility and validity of the proposed method, and realized the roll profile measurement with high precision.
文摘The term ‘profile machining’ is used to refer to the milling of vertical surfaces described by profile curves. Profile machining requires higher precision (1/1000 mm) than regular 3D machining (1/100 mm) with the erosion of sharp vertices should being especially avoided. Although, profile machining is very essential for making trimming and flange dies, it seldom brought into focus. This paper addresses the technological requirements of profile machining including machining width and depth control, minimizing toolware, and protecting sharp vertices. Issues of controller alarms are also addressed.
文摘Existing pressure drilling technologies are based on different principles and display distinct characteristics in terms of control pressure and degree of formation adaptability.In the present study,the constant-bottomhole-pressure(CBHP)and controlled-mud-level(CML)dual gradient drilling methods are considered.Models for the equivalent circulating density(ECD)are introduced for both drilling methods,taking into account the control pressure parameters(wellhead back pressure,displacement,mud level,etc.)and the relationship between the equivalent circulating density curve in the wellbore and two different types of pressure profiles in deep-water areas.The findings suggest that the main pressure control parameter for CBHP drilling is the wellhead back pressure,while for CML dual gradient drilling,it is the mud level.Two examples are considered(wells S1 and B2).For S1,CML dual gradient drilling only needs to adjust the ECD curve once to drill down to the target layer without risk.By comparison,CBHP drilling requires multiple adjustments to reach the target well depth avoiding a kick risk.In well B2,the CBHP method can drill down to the desired zone or even deeper after a single adjustment of the ECD curve.In contrast,CML dual-gradient drilling requires multiple adjustments to reach the target well depth(otherwise there is a risk of lost circulation).Therefore,CML dual-gradient drilling should be considered as a better choice for well S1,while CBHP drilling is more suitable for well B2.
文摘为研究辊形电磁调控技术(roll profile electromagnetic control technology,RPECT)的热力胀形行为,采用有限元方法分析了不同电流密度及频率下综合辊凸度、热力贡献辊凸度以及力凸度占比等随接触区热量的变化规律,探究了接触区热量与热力胀形能力的映射关系及其成立条件.结果表明,在接触区热量的中低取值范围内,同一接触区热量所对应的热力调控能力不会受磁参数影响,故提出将接触区热量作为输入参数与调控能力之间桥梁的RPECT高效控制策略.该策略通过提升磁参数来快速达到接触区的目标温升效果,进而获取目标调控能力.以20μm的辊缝凸度需求为例,经原始与高效控制策略间的对比发现,新策略的调控时间可有效地缩短至原调控时间的19.57%.
基金supported by Recruitment program of Global Youth Expert of China (to X.G.)the Elite Youth Program of the Chinese Academy of Agricultural Science (to X.G.)the intramural research support from Biotechnology Research Institute, Chinese Academy of Agricultural Sciences
文摘The most common and abundant DNA modification is 5-methylcytosine（5mC）,which has been well-established as an epigenetic mark regulating gene expression in eukaryotes（Jones,2012）.Another DNA modification N^6-methyldeoxyadenosine（6mA）,previously reported as a widespread DNA methylation in prokaryotes.
文摘In this report we have evaluated metabolite and RNA profiling technologies to begin to understand the natural variation in these biomolecules found in commercial-quality, conventional (non-GM) maize hybrids. Our analyses focus on mature grain, the article of commerce that is most typically subjected to the rigorous studies involved in the comparative safety assessment of GM products. We have used a population of conventionally-bred maize hybrids that derive from closely related inbred parents grown under standard field conditions across geographically similar locations. This study highlights the large amount of natural variation in metabolites and transcripts across conventional maize germplasm grown under normal field conditions, and underscores the critical need for further extensive studies before these technologies can be seriously considered for utility in the comparative safety assessment of GM crops.
