PLGA, m PEG diblock copolymer was synthesized by bulk ring-opening polymerization method. The double emulsion solvent evaporation method was used to prepare bovine serum albumin（BSA）-loaded microspheres. Optical mic...PLGA, m PEG diblock copolymer was synthesized by bulk ring-opening polymerization method. The double emulsion solvent evaporation method was used to prepare bovine serum albumin（BSA）-loaded microspheres. Optical microscopy was used to observe the whole microsphere fabrication process. It is confirmed that the proportion of inner aqueous phase is one of the most critical factors that determines the morphology of microspheres. Double emulsion droplets which have appropriate amount of inner aqueous phase can form closed and dense microspheres, while, too much inner aqueous phase will cause a collapse of the double emulsion droplets, resulting in a loss of drug. The proportion of inner aqueous phase was varied to prepare microspheres of different morphology. The results show that with increasing the amount of inner aqueous phase, a higher percent of broken microspheres and lower encapsulation efficiency appeared, and also, a more severe initial burst release and faster release rate.展开更多
Parameter estimation for ordinary differential equations arises in many fields of science and engineering. To be the best of our knowledge, traditional methods are often either computationally intensive or inaccurate ...Parameter estimation for ordinary differential equations arises in many fields of science and engineering. To be the best of our knowledge, traditional methods are often either computationally intensive or inaccurate for statistical inference. Ramsay et al.(2007) proposed a generalized profiling procedure. It is easily implementable and has been demonstrated to have encouraging numerical performance. However, little is known about statistical properties of this procedure. In this paper, we provide a theoretical justification of the generalized profiling procedure. Under some regularity conditions, the procedure is shown to be consistent for a broad range of tuning parameters. When the tuning parameters are sufficiently large, the procedure can be further shown to be asymptotically normal and efficient.展开更多
基金financially supported by the Independent Innovation Foundation of Huazhong University of Science and Technology(No.2013ZHYX008)the National Natural Science Foundation of China(No.81370980)
文摘PLGA, m PEG diblock copolymer was synthesized by bulk ring-opening polymerization method. The double emulsion solvent evaporation method was used to prepare bovine serum albumin（BSA）-loaded microspheres. Optical microscopy was used to observe the whole microsphere fabrication process. It is confirmed that the proportion of inner aqueous phase is one of the most critical factors that determines the morphology of microspheres. Double emulsion droplets which have appropriate amount of inner aqueous phase can form closed and dense microspheres, while, too much inner aqueous phase will cause a collapse of the double emulsion droplets, resulting in a loss of drug. The proportion of inner aqueous phase was varied to prepare microspheres of different morphology. The results show that with increasing the amount of inner aqueous phase, a higher percent of broken microspheres and lower encapsulation efficiency appeared, and also, a more severe initial burst release and faster release rate.
基金supported by National Science Foundation of USA (Grant Nos. DMS1209191 and DMS-1507511)
文摘Parameter estimation for ordinary differential equations arises in many fields of science and engineering. To be the best of our knowledge, traditional methods are often either computationally intensive or inaccurate for statistical inference. Ramsay et al.(2007) proposed a generalized profiling procedure. It is easily implementable and has been demonstrated to have encouraging numerical performance. However, little is known about statistical properties of this procedure. In this paper, we provide a theoretical justification of the generalized profiling procedure. Under some regularity conditions, the procedure is shown to be consistent for a broad range of tuning parameters. When the tuning parameters are sufficiently large, the procedure can be further shown to be asymptotically normal and efficient.
