Prebiotics synthesized by probiotic genera are of interest due to their specific selectivity.However,modifications of their composition and concentrations by digestion may affect their properties and effects in the ho...Prebiotics synthesized by probiotic genera are of interest due to their specific selectivity.However,modifications of their composition and concentrations by digestion may affect their properties and effects in the host.In the present study we assessed small intestinal digestibility of oligosaccharides synthesized by Acidipropionibacterium acidipropionici LET120-β-gal(β-galactosidase)from lactose(LET120-GOS),lactulose(LET120-OsLu)and lactitol(LET120-LOS)by incubating them with brush border membrane vesicles(BBMV)from pig and a rat small intestinal extract(RSIE)as in vitro models of mammalian mucosa.Both BBMVs and RSIE partially degraded the potential prebiotic oligosaccharides synthesized by dairy propionibacteria and hydrolysis was influenced by the structure and composition of the oligosaccharides.The highest degradation was observed for LET120-GOS,with 46.8%and 58.1%of hydrolysis after 5 h of digestion with BBMV and RSIE,respectively.On the contrary,LET120-OsLu and LET120-LOS showed higher resistance to intestinal degradation.β(1→6)linked products showed higher resistance to mammalian digestive enzymes compared toβ(1→4)andβ(1→3)linked oligosaccharides.Results emphasize the need of quantifying prebiotics that would reach the colon to anticipate their impact on health and the dose needed to exert that effect.展开更多
目的:考察大黄主要有效成分对痤疮主要致病菌的体外抑菌作用。方法:测定大黄的几种游离蒽醌对痤疮主要致病菌的最低抑菌浓度(M IC)。结果:大黄素对痤疮丙酸杆菌(Prop ion ibacteria Acnes)有较好的抑制作用,大黄酸对金黄色葡萄球菌(Stap...目的:考察大黄主要有效成分对痤疮主要致病菌的体外抑菌作用。方法:测定大黄的几种游离蒽醌对痤疮主要致病菌的最低抑菌浓度(M IC)。结果:大黄素对痤疮丙酸杆菌(Prop ion ibacteria Acnes)有较好的抑制作用,大黄酸对金黄色葡萄球菌(Staphylococcus aureus)有很强的抑制作用。结论:大黄的游离蒽醌对痤疮主要致病菌有较强的抑制作用。展开更多
基金supported by EMHE CSIC 2017,PID2021-123862OBI00 from MICINN,PICT-3504 from ANPCyT(Argentina)Project IC-901 from USPT(Argentina).
文摘Prebiotics synthesized by probiotic genera are of interest due to their specific selectivity.However,modifications of their composition and concentrations by digestion may affect their properties and effects in the host.In the present study we assessed small intestinal digestibility of oligosaccharides synthesized by Acidipropionibacterium acidipropionici LET120-β-gal(β-galactosidase)from lactose(LET120-GOS),lactulose(LET120-OsLu)and lactitol(LET120-LOS)by incubating them with brush border membrane vesicles(BBMV)from pig and a rat small intestinal extract(RSIE)as in vitro models of mammalian mucosa.Both BBMVs and RSIE partially degraded the potential prebiotic oligosaccharides synthesized by dairy propionibacteria and hydrolysis was influenced by the structure and composition of the oligosaccharides.The highest degradation was observed for LET120-GOS,with 46.8%and 58.1%of hydrolysis after 5 h of digestion with BBMV and RSIE,respectively.On the contrary,LET120-OsLu and LET120-LOS showed higher resistance to intestinal degradation.β(1→6)linked products showed higher resistance to mammalian digestive enzymes compared toβ(1→4)andβ(1→3)linked oligosaccharides.Results emphasize the need of quantifying prebiotics that would reach the colon to anticipate their impact on health and the dose needed to exert that effect.
