Prognostic analysis of prostaglandin D2 synthase in diffuse large B‑cell lymphoma

Purpose We aimed to analyse the correlation between prostaglandin D2 synthase(PTGDS)and a diffuse large B-cell lymphoma(DLBCL)prognosis.Methods We retrospectively collected two hundred paraffin-embedded tissue specimens that were pathologically diagnosed as DLBCL in the Fujian Tumour Hospital between January 2014 and December 2018.An abundance of paraffin-embedded tumour tissues were obtained.Twenty patients with lymphocyte-rich,benign,tissue-reactive,hypertrophic tonsillitis were selected as controls.Wax blocks were selected for primary cases and the controls were screened by professional pathologists.The levels of prostaglandin D2 synthase(PTGDS)and the EMT-related molecules,E-cadherin and vimentin,were detected by immunohistochemistry in clinical samples.A chi-square test revealed the correlations between PTGDS expression and clinicopathological characteristics,including age,sex,primary site,clinical stage,immunotyping,and International Prognostic Index(IPI)score.The Kaplan–Meier survival analysis was performed,and the diagnostic value was evaluated by receiver operating characteristic(ROC)curve analysis.Results A total of 138 cases(69%)were found to be PTGDS positive(>30%positive cells).PTGDS staining was negative(<30%positive cells)in 62 cases(31%).We collected the corresponding clinicopathological information and found that PTGDS expression was not significantly related to the patients’age,tumour stage,presence of extranodal invasion,or IPI score.According to the follow-up data,patients with low PTGDS expression had poor progression-free survival(PFS)and overall survival(OS),with 2-year PFS and OS rates of 41.7%and 50%,respectively.The 2-year PFS and OS rates of PTGDS-positive patients were 89.3%and 92.9%,respectively(P<0.0001),and the differences were significant.Conclusion We found that the expression level of PTGDS is significantly correlated with the prognosis of diffuse large B-cell lymphoma.