Objective:To study whether the infection of Schistosomiasis japanicum(S.japanicum) is related to enhanced proliferation and migration of cancer cells,and the molecular mechanism pertains to cancer cell metastasis in h...Objective:To study whether the infection of Schistosomiasis japanicum(S.japanicum) is related to enhanced proliferation and migration of cancer cells,and the molecular mechanism pertains to cancer cell metastasis in human host.Methods:The gene of S.japanicum glutathione transferase(sjGST) cloned from 5.japanicum was expressed,purified and applied in a series of assays to explore the effect of sjGST on proliferation and migration of MDA-MB-435S,and the expression of MMP2 and MMP9.Immunofluorescence assay for the binding of sjGST to MDA-MB-435S was also carried out.Results:Results showed that sjGST enhanced proliferation and migration in human breast cancer cell MDA-MB-435S signifycantly at 50-200 nM,but did not enhance them in human lung cancer cell A549.Immunofluorescence assay for the binding of sjGST to MDA-MB-435S and A549 showed that GST was readily hound to the breast cancer cells,but showed almost no binding to human lung cancer cells.The assays for gelatinase activity showed that both MMP2 and MMP9 activities were increased significantly in the presence of sjGST(50-200 nM) in MDA-MB-435S, but they were not significant in A549.Conclusions:Our current results show strongly that S. japanicum GST binds to MDA-MB-435S probably via its i'eceptor,and enhances proliferation and migration of the cancer cells by up-regulatory expression of MMP2 and MMP9.展开更多
Objective：The Wnt signaling pathway is crucial for pulmonary development and differentiation;dysregulation of the Wnt signaling pathway may impair lung function.Indeed,single nucleotide polymorphisms （SNPs） of Wnt ...Objective：The Wnt signaling pathway is crucial for pulmonary development and differentiation;dysregulation of the Wnt signaling pathway may impair lung function.Indeed,single nucleotide polymorphisms （SNPs） of Wnt pathway-related genes have been suggested as risk factors for certain types of cancers.In this study,we aimed to evaluate the influence of SNPs in Wnt-related genes （TCF2,MMP9） on susceptibility to lung cancer.Methods：Polymorphisms of TCF2 rs4430796,MMP9 rs2250889,and MMP9 rs17576 were studied in Han Chinese subjects,including 135 patients with lung cancer and 176 controls,using the Sequenom MassARRAY platform.The association of genotypes with susceptibility to lung cancer was analyzed using odds ratio （OR）,with 95% confidence interval （95% CI） and χ2.Results：The three SNPs （rs4430796,rs2250889,and rs17576） were found to be significantly associated with an increased risk of lung cancer.The AA genotype and AG＋AA genotype of rs4430796 showed a significantly increased susceptibility to lung cancer compared with the GG genotype （adjusted OR=6.03,95% CI：1.30-28.09,P=0.022;5.55,95% CI：1.20-25.58,P=0.028）.Compared with the rs17576 GG genotype,the AG and AG＋AA genotypes were also associated with a significant risk （adjusted OR=2.65,95% CI：1.60-4.37,P≤0.001;2.57,95% CI：1.59-4.19,P≤0.001） whereas the rs2250889 CG and CG＋GG genotypes had 2.97-fold （95% CI：1.81-4.85;P≤0.001） and 2.80-fold increased associations with lung cancer （95% CI：1.73-4.54;P≤0.001）,respectively,compared with the rs2250889 CC genotype.Furthermore,the association of rs4430796 with lung cancer became insignificant （P0.05） after adjusting for gender and rs2250889.Conclusion：The three SNPs may play a role in the predisposition of members of the Han Chinese population to lung cancer.展开更多
目的:研究蝎毒多肽提取物(peptide extract from scorpion venom,PESV)对雄激素非依赖性人前列腺癌细胞株DU-145COX-2和MMP-9表达的影响,进一步探讨其抗血管生成的分子机制,为抗前列腺癌骨转移提供有效的治疗手段。方法:采用免疫组只化...目的:研究蝎毒多肽提取物(peptide extract from scorpion venom,PESV)对雄激素非依赖性人前列腺癌细胞株DU-145COX-2和MMP-9表达的影响,进一步探讨其抗血管生成的分子机制,为抗前列腺癌骨转移提供有效的治疗手段。方法:采用免疫组只化学方法检测PESV对COX-2、MMP-9蛋白表达的影响,应用RT-PCR检测PESV对MMP-9在mRNA水平表达的影响。结果:蝎毒多肽提取物(40μg/mL)作用于前列腺癌细胞后,COX-2、MMP-9蛋白表达水平明显下调(P<0.05),进一步检测发现MMP-9在mRNA水平亦明显下降(P<0.05)。结论:蝎毒多肽提取物(PESV)通过抑制前列腺癌细胞血管生成因子COX-2的表达而发挥其抗血管生成作用,具有临床应用价值。展开更多
目的:探讨组蛋白甲基转移酶(enhancer of zeste homolog 2,EZH2),MMP-2和MMP-9在乳腺癌组织中的表达,并分析三者的表达与乳腺癌临床病理特征的关系。方法:采用免疫组织化学SP法检测147例乳腺癌、58例正常乳腺组织和58例乳腺良性肿瘤组织...目的:探讨组蛋白甲基转移酶(enhancer of zeste homolog 2,EZH2),MMP-2和MMP-9在乳腺癌组织中的表达,并分析三者的表达与乳腺癌临床病理特征的关系。方法:采用免疫组织化学SP法检测147例乳腺癌、58例正常乳腺组织和58例乳腺良性肿瘤组织中EZH2,MMP-2和MMP-9蛋白表达,分析三者间的关系。并将三者的表达与乳腺癌的临床病理特征进行相关性分析。结果:EZH2,MMP-2和MMP-9蛋白在乳腺癌组织中的表达显著高于正常乳腺组织及乳腺良性肿瘤组织(P均<0.001)。EZH2,MMP-2和MMP-9蛋白表达与乳腺癌患者年龄、绝经状态、肿瘤直径及组织学分类均无关,而与患者淋巴结转移有关(P<0.001)。结论:EZH2,MMP-2和MMP-9可能与乳腺癌的发生发展密切相关,三者联合检测可为乳腺癌的诊疗分析研究提供新的参考指标。展开更多
基金Supported by grants from National Science Council(NSC98-2314-B-110-001-MY3)
文摘Objective:To study whether the infection of Schistosomiasis japanicum(S.japanicum) is related to enhanced proliferation and migration of cancer cells,and the molecular mechanism pertains to cancer cell metastasis in human host.Methods:The gene of S.japanicum glutathione transferase(sjGST) cloned from 5.japanicum was expressed,purified and applied in a series of assays to explore the effect of sjGST on proliferation and migration of MDA-MB-435S,and the expression of MMP2 and MMP9.Immunofluorescence assay for the binding of sjGST to MDA-MB-435S was also carried out.Results:Results showed that sjGST enhanced proliferation and migration in human breast cancer cell MDA-MB-435S signifycantly at 50-200 nM,but did not enhance them in human lung cancer cell A549.Immunofluorescence assay for the binding of sjGST to MDA-MB-435S and A549 showed that GST was readily hound to the breast cancer cells,but showed almost no binding to human lung cancer cells.The assays for gelatinase activity showed that both MMP2 and MMP9 activities were increased significantly in the presence of sjGST(50-200 nM) in MDA-MB-435S, but they were not significant in A549.Conclusions:Our current results show strongly that S. japanicum GST binds to MDA-MB-435S probably via its i'eceptor,and enhances proliferation and migration of the cancer cells by up-regulatory expression of MMP2 and MMP9.
基金supported by the Key Programs for Science and Technology Development of Guangzhou (No. 2008A1-E4151)the National "863" High Technology Research and Development Program of China (No. 2006AA02A311)
文摘Objective：The Wnt signaling pathway is crucial for pulmonary development and differentiation;dysregulation of the Wnt signaling pathway may impair lung function.Indeed,single nucleotide polymorphisms （SNPs） of Wnt pathway-related genes have been suggested as risk factors for certain types of cancers.In this study,we aimed to evaluate the influence of SNPs in Wnt-related genes （TCF2,MMP9） on susceptibility to lung cancer.Methods：Polymorphisms of TCF2 rs4430796,MMP9 rs2250889,and MMP9 rs17576 were studied in Han Chinese subjects,including 135 patients with lung cancer and 176 controls,using the Sequenom MassARRAY platform.The association of genotypes with susceptibility to lung cancer was analyzed using odds ratio （OR）,with 95% confidence interval （95% CI） and χ2.Results：The three SNPs （rs4430796,rs2250889,and rs17576） were found to be significantly associated with an increased risk of lung cancer.The AA genotype and AG＋AA genotype of rs4430796 showed a significantly increased susceptibility to lung cancer compared with the GG genotype （adjusted OR=6.03,95% CI：1.30-28.09,P=0.022;5.55,95% CI：1.20-25.58,P=0.028）.Compared with the rs17576 GG genotype,the AG and AG＋AA genotypes were also associated with a significant risk （adjusted OR=2.65,95% CI：1.60-4.37,P≤0.001;2.57,95% CI：1.59-4.19,P≤0.001） whereas the rs2250889 CG and CG＋GG genotypes had 2.97-fold （95% CI：1.81-4.85;P≤0.001） and 2.80-fold increased associations with lung cancer （95% CI：1.73-4.54;P≤0.001）,respectively,compared with the rs2250889 CC genotype.Furthermore,the association of rs4430796 with lung cancer became insignificant （P0.05） after adjusting for gender and rs2250889.Conclusion：The three SNPs may play a role in the predisposition of members of the Han Chinese population to lung cancer.
文摘目的:研究蝎毒多肽提取物(peptide extract from scorpion venom,PESV)对雄激素非依赖性人前列腺癌细胞株DU-145COX-2和MMP-9表达的影响,进一步探讨其抗血管生成的分子机制,为抗前列腺癌骨转移提供有效的治疗手段。方法:采用免疫组只化学方法检测PESV对COX-2、MMP-9蛋白表达的影响,应用RT-PCR检测PESV对MMP-9在mRNA水平表达的影响。结果:蝎毒多肽提取物(40μg/mL)作用于前列腺癌细胞后,COX-2、MMP-9蛋白表达水平明显下调(P<0.05),进一步检测发现MMP-9在mRNA水平亦明显下降(P<0.05)。结论:蝎毒多肽提取物(PESV)通过抑制前列腺癌细胞血管生成因子COX-2的表达而发挥其抗血管生成作用,具有临床应用价值。
文摘目的:探讨卡培他滨联合常规化疗治疗晚期胃癌疗效、不良反应及对血清基质金属蛋白酶2(matrix metalloproteinase 2,MMP-2)及MMP-9的影响,为临床治疗晚期胃癌提供临床依据.方法:收集南华大学附属第二医院2011-10/2013-10收治的120例胃癌患者,随机将其分为对照组57例与观察组63例.对照组给予常规多西紫杉醇和顺铂治疗,观察组在常规治疗基础上给予卡培他滨治疗,观察并比较两组患者疗效、生存率、不良反应及对血清MMP-2和MMP-9水平的影响.结果:两组患者治疗有效率比较差异无统计学意义(P>0.05),但观察组6 mo与1年生存率分别为88.9%与77.8%,均显著高于对照组患者(73.7%vs 59.6%),差异具有统计学意义(P<0.05).观察组患者在骨髓抑制及胃肠道反应方面显著高于对照组患者,差异具有统计学意义(P<0.05),两组患者其他方面不良反应差异无统计学意义.两组治疗后与治疗前相比,血清MMP-2与MMP-9水平均有所降低,差异均具有统计学意义(P<0.05).与对照组相比,观察组治疗后MMP-2与MMP-9均显著低于对照组治疗后血清水平,差异均具有统计学意义(P<0.05).结论:卡培他滨联合常规化疗治疗晚期胃癌疗效确切,安全性较好,并且可显著降低M M P-2与M M P-9的血清水平,值得临床上进一步深入研究.
文摘目的:探讨组蛋白甲基转移酶(enhancer of zeste homolog 2,EZH2),MMP-2和MMP-9在乳腺癌组织中的表达,并分析三者的表达与乳腺癌临床病理特征的关系。方法:采用免疫组织化学SP法检测147例乳腺癌、58例正常乳腺组织和58例乳腺良性肿瘤组织中EZH2,MMP-2和MMP-9蛋白表达,分析三者间的关系。并将三者的表达与乳腺癌的临床病理特征进行相关性分析。结果:EZH2,MMP-2和MMP-9蛋白在乳腺癌组织中的表达显著高于正常乳腺组织及乳腺良性肿瘤组织(P均<0.001)。EZH2,MMP-2和MMP-9蛋白表达与乳腺癌患者年龄、绝经状态、肿瘤直径及组织学分类均无关,而与患者淋巴结转移有关(P<0.001)。结论:EZH2,MMP-2和MMP-9可能与乳腺癌的发生发展密切相关,三者联合检测可为乳腺癌的诊疗分析研究提供新的参考指标。
