Electric vehicles(EVs)have garnered significant attention as a vital driver of economic growth and environmental sustainability.Nevertheless,ensuring the safety of high-energy batteries is now a top priority that cann...Electric vehicles(EVs)have garnered significant attention as a vital driver of economic growth and environmental sustainability.Nevertheless,ensuring the safety of high-energy batteries is now a top priority that cannot be overlooked during large-scale applications.This paper proposes an innovative active protection and cooling integrated battery module using smart materials,magneto-sensitive shear thickening fluid(MSTF),which is specifically designed to address safety threats posed by lithium-ion batteries(LIBs)exposed to harsh mechanical and environmental conditions.The theoretical framework introduces a novel approach for harnessing the smoothed-particle hydrodynamics(SPH)methodology that incorporates the intricate interplay of non-Newtonian fluid behavior,capturing the fluid-structure coupling inherent to the MSTF.This approach is further advanced by adopting an enhanced Herschel-Bulkley(H-B)model to encapsulate the intricate rheology of the MSTF under the influence of the magnetorheological effect(MRE)and shear thickening(ST)behavior.Numerical simulation results show that in the case of cooling,the MSTF is an effective cooling medium for rapidly reducing the temperature.In terms of mechanical abuse,the MSTF solidifies through actively applying the magnetic field during mechanical compression and impact within the battery module,resulting in 66%and 61.7%reductions in the maximum stress within the battery jellyroll,and 31.1%and 23%reductions in the reaction force,respectively.This mechanism effectively lowers the risk of short-circuit failure.The groundbreaking concepts unveiled in this paper for active protection battery modules are anticipated to be a valuable technological breakthrough in the areas of EV safety and lightweight/integrated design.展开更多
The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strateg...The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strategies, which are hampered by the weight and size of inspection equipment, as well as the taxonomic extensiveness of interrupted species. Moreover, some insect pests that impede farmer productivity and profitability are difficult for researchers to address on time due to a lack of appropriate plant protection measures. Farmers’ reliance on synthetic pesticides and biocontrol agents has resulted in major economic and environmental ramifications. DNA barcoding is a novel technology that has the potential to improve Integrated Pest Management decision-making, which is dependent on the ability to correctly identify pest and beneficial organisms. This is due to some natural traits such as phenology or pesticide susceptibility browbeaten by IPM strategies to avert pest establishment. Specifically, Deoxyribonucleic acid (DNA) sequence information was applied effectively for the identification of some micro-organisms. This technology, DNA barcoding, allows for the identification of insect species by using short, standardized gene sequences. DNA barcoding is basically based on repeatable and accessible technique that allows for the mechanisation or automation of species discrimination. This technique bridges the taxonomic bio-security gap and meets the International Plant Protection Convention diagnostic standards for insect identification. This review therefore discusses DNA barcoding as a technique for insect pests’ identification and its potential application for crop protection.展开更多
Local color is not only the cultural counter in the competition between cities,but the main cultural element that attracts foreign tourists.In recent years,many cities have regarded tourism development as a major mean...Local color is not only the cultural counter in the competition between cities,but the main cultural element that attracts foreign tourists.In recent years,many cities have regarded tourism development as a major mean of reviving ancient towns.The excavation of local culture,material reconstruction of local characteristics,and the resurgence and"reproduction"of local industries have become an important work in the tourism development of ancient towns.Apart from the discussion of the protection,inheritance,and touristification of tangible cultural heritage and intangible cultural heritage,this paper demonstrated the possibility of the integration and production capacity of local characteristic industry resources and analyzed the impact of the integrated development on the lifestyle and living atmosphere of residents in the ancient towns.In this paper,ways to realize the integrated development of the tourism and protection of ancient towns were included as follows:(1)to ensure the principal status of local residents and give full play to their initiatives;(2)to protect the local traditional way of life and improve the public service system;(3)to choose the appropriate development pattern and achieve the diversified development;(4)to improve service facilities in tourism and adjust the structure of tourism products.展开更多
To explore new light-weight integrated thermal protection system panel configuration and gain good insight into the responses mechanism,heat transfer and structural field analysis for one single-layer and four double-...To explore new light-weight integrated thermal protection system panel configuration and gain good insight into the responses mechanism,heat transfer and structural field analysis for one single-layer and four double-layer corrugated core panels were performed. The obtained the temperature,buckling,stress and deflection responses were compared,and the deflection and stress distributions as well as thermal buckling mode at the time were discussed for the considered configurations when the temperature difference between the top and bottom face sheet was maximum. The results demonstrated that the non-orthogonal and hat-stiffened double-layer structures provide superior performance to resist thermal buckling deformation in comparison with other configurations. The useful information is provided for the forthcoming optimization in which thermal buckling is considered as critical design driver.展开更多
Farmland and primary farmland protection are important contents of land management and land use planning of China. In the new round of land use planning and database construction, primary farmland protection zones are...Farmland and primary farmland protection are important contents of land management and land use planning of China. In the new round of land use planning and database construction, primary farmland protection zones are required to have high integrity and connectivity. Using landscape pattern indexes, the integrity and connectivity of primary farmland protection zones was studied in Licheng District of Jinan City. The results showed that, except patch area standard deviation, the other indexes including average patch area, patch area variation coefficient, patch edge density, largest patch index, and mean euclidean nearest-neighbor, all indicate high connectivity of primary farmland protection zones after layout adjustment. A simple and convenient method for identifying the integrity and connectivity of primary farmland protection zones was supplied.展开更多
With the continuous progress of the comprehensive implementation of the rural revitalization strategy, the protection of famous historical and cultural cities, towns and villages has received great attention from the ...With the continuous progress of the comprehensive implementation of the rural revitalization strategy, the protection of famous historical and cultural cities, towns and villages has received great attention from the state and society. With the continuous improvement of the level of economic development and the further advancement of industrialization and urbanization, the current historical and cultural environment and ecological environment are increasingly eroded and destroyed. From the perspective of cultural ecology, based on exploring the cultural ecological characteristics of Sanggan River Basin, this paper classified and analyzed the historical and cultural cities, towns and villages in the basin according to three levels: natural ecological elements, historical functions of towns and historical function forms of villages, and further explored their overall protection methods, hoping to provide some reference for the protection of other famous historical and cultural cities, towns and villages in China.展开更多
Peking Union Medical College(PUMC),as the representative work of Chinese modern architecture,has had a great impact on the development of Chinese modern architecture.Its advanced and complete concealed works contain g...Peking Union Medical College(PUMC),as the representative work of Chinese modern architecture,has had a great impact on the development of Chinese modern architecture.Its advanced and complete concealed works contain great value and are valuable materials for the study of Chinese modern architecture.This paper studies the concealed works of PUMC,enriches the research data in this field,and summarizes the transformation design scheme of concealed works in the protection and repair of ancient buildings of PUMC.展开更多
"…enable Jiangxi to become the example of ecological civilization and pacesetter of building beautiful China,and realize the dream of a prosperous,harmonious and beautiful Jiangxi through our earnest efforts.&qu..."…enable Jiangxi to become the example of ecological civilization and pacesetter of building beautiful China,and realize the dream of a prosperous,harmonious and beautiful Jiangxi through our earnest efforts."Recent years have witnessed vigorous efforts made by the Provincial Foreign Affairs and Overseas Chinese展开更多
Definitions and features of eco-park and theme park were introduced as well as their differences, it was proposed that by applying cultural themes suited to regional ecological resources in the construction of eco-par...Definitions and features of eco-park and theme park were introduced as well as their differences, it was proposed that by applying cultural themes suited to regional ecological resources in the construction of eco-park, the eco-park could achieve not only desired ecological benefits, but also social and economic benefits, and the optimal comprehensive benefits. Design of Baiyunzhai Demonstration Eco-park was taken for an example, integration of eco-park and farming culture was confirmed as the nature, principles of "adaptation to actual conditions, amended protection and construction of plant system" proposed, three functional areas of "Colorful Forest Belt, Yiyun Health Maintenance Zone, Lakefront Meditation Zone" are given on the basis of actual terrains, planning highlights and desired landscape effects expounded to promote the value of regional cultures in Chongqing by building Baiyunzhai Eco-park.展开更多
Phaeocystis globosa Scherffel is one of the common harmful algae species in coastal waters of the southeastern China.In this study,sandwich hybridization integrated with nuclease protection assay(NPA-SH)was used to qu...Phaeocystis globosa Scherffel is one of the common harmful algae species in coastal waters of the southeastern China.In this study,sandwich hybridization integrated with nuclease protection assay(NPA-SH)was used to qualitatively and quantitatively detect P. globosa.Results showed that this method had good applicability and validity in analyzing the samples from laboratory cultures and from fields.The linear regression equation for P.globosa was obtained,and the lowest detection number of cells was 1.8×104 c...展开更多
AIM:To assess whether glutamate plays a similar role to glutamine in preserving gut wall integrity.METHODS:The effects of glutamine and glutamate on induced hyperpermeability in intestinal cell lines were studied.Para...AIM:To assess whether glutamate plays a similar role to glutamine in preserving gut wall integrity.METHODS:The effects of glutamine and glutamate on induced hyperpermeability in intestinal cell lines were studied.Paracellular hyperpermeability was induced in Caco2.BBE and HT-29CL.19A cell lines by adding phorbol-12,13-dibutyrate(PDB) apically,after which the effects of glutamine and glutamate on horseradish peroxidase(HRP) diffusion were studied.An inhibitor of glutamate transport(L-trans-pyrrolidine-2,4-dicarboxylic acid:trans-PDC) and an irreversible blocker(acivicin) of the extracellular glutamine to glutamate converting enzyme,γ-glutamyltransferase,were used.RESULTS:Apical to basolateral HRP flux increased significantly compared to controls not exposed to PDB (n=30,P<0.001).Glutamine application reduced hyperpermeability by 19%and 39%in the respective cell lines.Glutamate application reduced hyperpermeability by 30%and 20%,respectively.Incubation of HT29CL.19A cells with acivicin and subsequent PDB and glutamine addition increased permeability levels.Incubation of Caco2.BBE cells with trans-PDC followed by PDB and glutamate addition also resulted in high permeability levels.CONCLUSION:Apical glutamate-similar to glutaminecan decrease induced paracellular hyperpermeability.Extracellular conversion of glutamine to glutamate and subsequent uptake of glutamate could be a pivotal step in the mechanism underlying the protective effect of glutamine.展开更多
Prorocentrum donghaiense is an important harmful algae bloom (HAB) causing creature in China's seas, and the conventional visual detection can not cope with long-term monitoring and highthroughput sampling projects...Prorocentrum donghaiense is an important harmful algae bloom (HAB) causing creature in China's seas, and the conventional visual detection can not cope with long-term monitoring and highthroughput sampling projects. An assay for P. donghaiense with sandwich hybridization integrated with nuclease protection assay (NPA-SH) was established. Tests with mixed samples and spiked field ones confirmed its good specificity and sensitivity. The cell number of P. donghaiense correlated well with the optical density, and the regression equation is y=4× 10^- 6x+ 0.694 9, in which x is the cell number, and y is the optical density, with r2=0.953 5. These results show that the NPA-SH method has good feasibility in the detection of P. donghaiense. Results of NPA-SH and microscopy are excellent for each sample. The NPA-SH method was a simple way in quantitative detection of P. donghaiense, and the whole process could be finished in about six hours, which provided a new approach in high-throughput sampling and long-term monitoring of P. donghaiense.展开更多
Bacillus thuringiensis is a major microbial insecticide and a source of genes encoding several proteins toxic to insects. In this paper the authors g ive a brief summary of Bacillus thuringiensis used on the integrat...Bacillus thuringiensis is a major microbial insecticide and a source of genes encoding several proteins toxic to insects. In this paper the authors g ive a brief summary of Bacillus thuringiensis used on the integrated pest manage ment in forestry. The derivatives of Bt strain HD1 subsp kurstaki have been wide ly used to control the forest pests such as the gypsy moth (Lymantria dispar), s pruce budworm (Choristoneura fumiferana), the pine processionary moth (Thaumetop oea pityocampa), the European pine shoot moth (Rhyacionia buoliana) and the nun moth (Lymantria monacha). Some progresses of transferring and expressing Bt toxi n gene in forest trees are offered with a discussion on the limits and future pr ospects of using Bt products in forestry.展开更多
According to the Annex Technical Regulations for Integrated Curriculum Development(Trial)in Document No.30 of the General Office of the Ministry of Human Resources and Social Security(2012),this paper studies the form...According to the Annex Technical Regulations for Integrated Curriculum Development(Trial)in Document No.30 of the General Office of the Ministry of Human Resources and Social Security(2012),this paper studies the formulation of the curriculum standards for the integration of Chinese medicinal materials production.We focus on the formulation ideas of the curriculum standards for the integration of Chinese medicinal materials production,the formulation process of the curriculum standards for the integration of Chinese medicinal materials production,including the description of typical work tasks,the determination of curriculum objectives,the analysis of study content,the description of referential study tasks,teaching implementation suggestions,assessment and evaluation suggestions,which can provide a reference for the development and research of other related integrated courses.展开更多
The evolvement of IPM has experienced several significant stages. IPM is an ecosystem as a governance unit. It allows the presence of low numbers of pests because a direct or indirect relationship between biological s...The evolvement of IPM has experienced several significant stages. IPM is an ecosystem as a governance unit. It allows the presence of low numbers of pests because a direct or indirect relationship between biological species and their associated environmental living conditions could keep a long-term ecological balance. IPM also emphasizes overall benefits; it is necessary to integrate all kinds of safe, effective, economic, and simple managements as far as possible. When synthesizing and coordinating various managements, effective control object, effective time limit, effective scope, and effective degree of the individual method should be analysed first. The ultimate goal of IPM is for the sustainable development of agriculture. In the future IPM system engineering, crop resistance, the role of natural enemies, agricultural control, and the application of biological control technologies will be greatly strengthened, and the role of chemical pesticides will be significantly reduced. Safe, effective, and long-lasting control of pest populations will be below the level of harm caused, which could protect the ecological environment and the health of people and livestock, and promote the development of production.展开更多
Proton therapy is the most advanced radiotherapy approach in the world,and causes less damage to normal human tissue than traditional radiotherapy.Because the treatment process produces a high-energy proton beam,the p...Proton therapy is the most advanced radiotherapy approach in the world,and causes less damage to normal human tissue than traditional radiotherapy.Because the treatment process produces a high-energy proton beam,the personnel safety interlock system mainly considers measures to protect personnel from radiation hazards during beam preparation and the beam release process.Unlike other safety interlock systems,the personnel safety interlock system designed in this study focuses on the safety and stability of the system itself.The hardware and software of important interlock control loops are designed and developed according to the requirements of Safety Integrity Level 3 specified by IEC61508.A set of redundant ring networks was developed to ensure that damage to a certain network line does not affect the normal operation of the system.A set of friendly operation interfaces and data storage systems were developed to ensure that the operator can monitor the data in real time and trace the data.The personnel safety interlock system mainly includes a beam enabling function,clearance function,and emergency stop function.The system was put into actual use and successfully ensured personnel safety.展开更多
The reliability analysis of vertically integrated protection devices is crucial for designing International Electrotechnical Commission(IEC)61850-based substations.This paper presents the hardware architecture of a fo...The reliability analysis of vertically integrated protection devices is crucial for designing International Electrotechnical Commission(IEC)61850-based substations.This paper presents the hardware architecture of a four-inone vertically integrated device and the information transmission path of each function based on the functional information transmission chain of protection devices,measurement and control devices,merging units,and intelligent terminals.Additionally,a reliability analysis model of the protection device and its protection system is constructed using the fault tree analysis method while considering the characteristics of each module of the vertically integrated device.The stability probability of the protection system in each state is analyzed by combining the state-transfer equations of line and busbar protection with a Markov chain.Finally,the failure rate and availability of the protection device and its protection system are calculated under different ambient temperatures using a 110 kV intelligent substation as an example.The sensitivity of each device module is analyzed.展开更多
This paper presents an integrated protection technique for power distribution systems. A novel integrated protection scheme based on overcurrent protection technique for distribution system is described, in which a sp...This paper presents an integrated protection technique for power distribution systems. A novel integrated protection scheme based on overcurrent protection technique for distribution system is described, in which a specially designed protection relay is installed at each substation of a network and responsible for the protection of every line sections connected to the substation busbar The conventional directional overcurrent and the new adaptive accelerated protection algorithms with multiple settings are implemented into the relay to cover all the protected line sections. The paper includes studies of a typical multi section distribution network to demonstrate the principle of the scheme. Studies show that the new scheme not only offer the new protection features for individual power line section, but also provide the characteristics of integrated protection.展开更多
基金Project supported by the National Natural Science Foundation of China(Nos.12072183 and11872236)the Key Research Project of Zhejiang Laboratory(No.2021PE0AC02)。
文摘Electric vehicles(EVs)have garnered significant attention as a vital driver of economic growth and environmental sustainability.Nevertheless,ensuring the safety of high-energy batteries is now a top priority that cannot be overlooked during large-scale applications.This paper proposes an innovative active protection and cooling integrated battery module using smart materials,magneto-sensitive shear thickening fluid(MSTF),which is specifically designed to address safety threats posed by lithium-ion batteries(LIBs)exposed to harsh mechanical and environmental conditions.The theoretical framework introduces a novel approach for harnessing the smoothed-particle hydrodynamics(SPH)methodology that incorporates the intricate interplay of non-Newtonian fluid behavior,capturing the fluid-structure coupling inherent to the MSTF.This approach is further advanced by adopting an enhanced Herschel-Bulkley(H-B)model to encapsulate the intricate rheology of the MSTF under the influence of the magnetorheological effect(MRE)and shear thickening(ST)behavior.Numerical simulation results show that in the case of cooling,the MSTF is an effective cooling medium for rapidly reducing the temperature.In terms of mechanical abuse,the MSTF solidifies through actively applying the magnetic field during mechanical compression and impact within the battery module,resulting in 66%and 61.7%reductions in the maximum stress within the battery jellyroll,and 31.1%and 23%reductions in the reaction force,respectively.This mechanism effectively lowers the risk of short-circuit failure.The groundbreaking concepts unveiled in this paper for active protection battery modules are anticipated to be a valuable technological breakthrough in the areas of EV safety and lightweight/integrated design.
文摘The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strategies, which are hampered by the weight and size of inspection equipment, as well as the taxonomic extensiveness of interrupted species. Moreover, some insect pests that impede farmer productivity and profitability are difficult for researchers to address on time due to a lack of appropriate plant protection measures. Farmers’ reliance on synthetic pesticides and biocontrol agents has resulted in major economic and environmental ramifications. DNA barcoding is a novel technology that has the potential to improve Integrated Pest Management decision-making, which is dependent on the ability to correctly identify pest and beneficial organisms. This is due to some natural traits such as phenology or pesticide susceptibility browbeaten by IPM strategies to avert pest establishment. Specifically, Deoxyribonucleic acid (DNA) sequence information was applied effectively for the identification of some micro-organisms. This technology, DNA barcoding, allows for the identification of insect species by using short, standardized gene sequences. DNA barcoding is basically based on repeatable and accessible technique that allows for the mechanisation or automation of species discrimination. This technique bridges the taxonomic bio-security gap and meets the International Plant Protection Convention diagnostic standards for insect identification. This review therefore discusses DNA barcoding as a technique for insect pests’ identification and its potential application for crop protection.
基金Sponsored by 2016 Cultural Research Fund of the Publicity Department of Chaozhou(Z16105)
文摘Local color is not only the cultural counter in the competition between cities,but the main cultural element that attracts foreign tourists.In recent years,many cities have regarded tourism development as a major mean of reviving ancient towns.The excavation of local culture,material reconstruction of local characteristics,and the resurgence and"reproduction"of local industries have become an important work in the tourism development of ancient towns.Apart from the discussion of the protection,inheritance,and touristification of tangible cultural heritage and intangible cultural heritage,this paper demonstrated the possibility of the integration and production capacity of local characteristic industry resources and analyzed the impact of the integrated development on the lifestyle and living atmosphere of residents in the ancient towns.In this paper,ways to realize the integrated development of the tourism and protection of ancient towns were included as follows:(1)to ensure the principal status of local residents and give full play to their initiatives;(2)to protect the local traditional way of life and improve the public service system;(3)to choose the appropriate development pattern and achieve the diversified development;(4)to improve service facilities in tourism and adjust the structure of tourism products.
基金Sponsored by the National Natural Science Foundation of China(Grant No.11102054)Postdoctoral Science-research Developmental Foundation of Heilongjiang Province(Grant No.LBH-Q12101)the Fundamental Research Funds for the Central Universities(Grant No.HIT.NSRIF.2014026)
文摘To explore new light-weight integrated thermal protection system panel configuration and gain good insight into the responses mechanism,heat transfer and structural field analysis for one single-layer and four double-layer corrugated core panels were performed. The obtained the temperature,buckling,stress and deflection responses were compared,and the deflection and stress distributions as well as thermal buckling mode at the time were discussed for the considered configurations when the temperature difference between the top and bottom face sheet was maximum. The results demonstrated that the non-orthogonal and hat-stiffened double-layer structures provide superior performance to resist thermal buckling deformation in comparison with other configurations. The useful information is provided for the forthcoming optimization in which thermal buckling is considered as critical design driver.
基金Sponsored by Land Use Planning Database Construction Program of Licheng District,Jinan City
文摘Farmland and primary farmland protection are important contents of land management and land use planning of China. In the new round of land use planning and database construction, primary farmland protection zones are required to have high integrity and connectivity. Using landscape pattern indexes, the integrity and connectivity of primary farmland protection zones was studied in Licheng District of Jinan City. The results showed that, except patch area standard deviation, the other indexes including average patch area, patch area variation coefficient, patch edge density, largest patch index, and mean euclidean nearest-neighbor, all indicate high connectivity of primary farmland protection zones after layout adjustment. A simple and convenient method for identifying the integrity and connectivity of primary farmland protection zones was supplied.
文摘With the continuous progress of the comprehensive implementation of the rural revitalization strategy, the protection of famous historical and cultural cities, towns and villages has received great attention from the state and society. With the continuous improvement of the level of economic development and the further advancement of industrialization and urbanization, the current historical and cultural environment and ecological environment are increasingly eroded and destroyed. From the perspective of cultural ecology, based on exploring the cultural ecological characteristics of Sanggan River Basin, this paper classified and analyzed the historical and cultural cities, towns and villages in the basin according to three levels: natural ecological elements, historical functions of towns and historical function forms of villages, and further explored their overall protection methods, hoping to provide some reference for the protection of other famous historical and cultural cities, towns and villages in China.
文摘Peking Union Medical College(PUMC),as the representative work of Chinese modern architecture,has had a great impact on the development of Chinese modern architecture.Its advanced and complete concealed works contain great value and are valuable materials for the study of Chinese modern architecture.This paper studies the concealed works of PUMC,enriches the research data in this field,and summarizes the transformation design scheme of concealed works in the protection and repair of ancient buildings of PUMC.
文摘"…enable Jiangxi to become the example of ecological civilization and pacesetter of building beautiful China,and realize the dream of a prosperous,harmonious and beautiful Jiangxi through our earnest efforts."Recent years have witnessed vigorous efforts made by the Provincial Foreign Affairs and Overseas Chinese
文摘Definitions and features of eco-park and theme park were introduced as well as their differences, it was proposed that by applying cultural themes suited to regional ecological resources in the construction of eco-park, the eco-park could achieve not only desired ecological benefits, but also social and economic benefits, and the optimal comprehensive benefits. Design of Baiyunzhai Demonstration Eco-park was taken for an example, integration of eco-park and farming culture was confirmed as the nature, principles of "adaptation to actual conditions, amended protection and construction of plant system" proposed, three functional areas of "Colorful Forest Belt, Yiyun Health Maintenance Zone, Lakefront Meditation Zone" are given on the basis of actual terrains, planning highlights and desired landscape effects expounded to promote the value of regional cultures in Chongqing by building Baiyunzhai Eco-park.
基金supported by the National Hi-Tech Research and Development Program(863)of China(No.2006AA09Z178,2001AA635090)the National Nat-ural Science Foundation of China(No.40706044)
文摘Phaeocystis globosa Scherffel is one of the common harmful algae species in coastal waters of the southeastern China.In this study,sandwich hybridization integrated with nuclease protection assay(NPA-SH)was used to qualitatively and quantitatively detect P. globosa.Results showed that this method had good applicability and validity in analyzing the samples from laboratory cultures and from fields.The linear regression equation for P.globosa was obtained,and the lowest detection number of cells was 1.8×104 c...
基金Supported by VU University Medical Center,Amsterdam,The Netherlands
文摘AIM:To assess whether glutamate plays a similar role to glutamine in preserving gut wall integrity.METHODS:The effects of glutamine and glutamate on induced hyperpermeability in intestinal cell lines were studied.Paracellular hyperpermeability was induced in Caco2.BBE and HT-29CL.19A cell lines by adding phorbol-12,13-dibutyrate(PDB) apically,after which the effects of glutamine and glutamate on horseradish peroxidase(HRP) diffusion were studied.An inhibitor of glutamate transport(L-trans-pyrrolidine-2,4-dicarboxylic acid:trans-PDC) and an irreversible blocker(acivicin) of the extracellular glutamine to glutamate converting enzyme,γ-glutamyltransferase,were used.RESULTS:Apical to basolateral HRP flux increased significantly compared to controls not exposed to PDB (n=30,P<0.001).Glutamine application reduced hyperpermeability by 19%and 39%in the respective cell lines.Glutamate application reduced hyperpermeability by 30%and 20%,respectively.Incubation of HT29CL.19A cells with acivicin and subsequent PDB and glutamine addition increased permeability levels.Incubation of Caco2.BBE cells with trans-PDC followed by PDB and glutamate addition also resulted in high permeability levels.CONCLUSION:Apical glutamate-similar to glutaminecan decrease induced paracellular hyperpermeability.Extracellular conversion of glutamine to glutamate and subsequent uptake of glutamate could be a pivotal step in the mechanism underlying the protective effect of glutamine.
基金The National High Technology Research and Development Program ("863" Program) of China under contract No 2006AA09Z178 the National Natural Science Foundation of China under contract No 40706044
文摘Prorocentrum donghaiense is an important harmful algae bloom (HAB) causing creature in China's seas, and the conventional visual detection can not cope with long-term monitoring and highthroughput sampling projects. An assay for P. donghaiense with sandwich hybridization integrated with nuclease protection assay (NPA-SH) was established. Tests with mixed samples and spiked field ones confirmed its good specificity and sensitivity. The cell number of P. donghaiense correlated well with the optical density, and the regression equation is y=4× 10^- 6x+ 0.694 9, in which x is the cell number, and y is the optical density, with r2=0.953 5. These results show that the NPA-SH method has good feasibility in the detection of P. donghaiense. Results of NPA-SH and microscopy are excellent for each sample. The NPA-SH method was a simple way in quantitative detection of P. donghaiense, and the whole process could be finished in about six hours, which provided a new approach in high-throughput sampling and long-term monitoring of P. donghaiense.
文摘Bacillus thuringiensis is a major microbial insecticide and a source of genes encoding several proteins toxic to insects. In this paper the authors g ive a brief summary of Bacillus thuringiensis used on the integrated pest manage ment in forestry. The derivatives of Bt strain HD1 subsp kurstaki have been wide ly used to control the forest pests such as the gypsy moth (Lymantria dispar), s pruce budworm (Choristoneura fumiferana), the pine processionary moth (Thaumetop oea pityocampa), the European pine shoot moth (Rhyacionia buoliana) and the nun moth (Lymantria monacha). Some progresses of transferring and expressing Bt toxi n gene in forest trees are offered with a discussion on the limits and future pr ospects of using Bt products in forestry.
基金Supported by Scientific Research Fund Project of Yunnan Provincial Department of Education (2023J2034).
文摘According to the Annex Technical Regulations for Integrated Curriculum Development(Trial)in Document No.30 of the General Office of the Ministry of Human Resources and Social Security(2012),this paper studies the formulation of the curriculum standards for the integration of Chinese medicinal materials production.We focus on the formulation ideas of the curriculum standards for the integration of Chinese medicinal materials production,the formulation process of the curriculum standards for the integration of Chinese medicinal materials production,including the description of typical work tasks,the determination of curriculum objectives,the analysis of study content,the description of referential study tasks,teaching implementation suggestions,assessment and evaluation suggestions,which can provide a reference for the development and research of other related integrated courses.
基金Supported by China Agricultural Industry Research System(CARS-12)
文摘The evolvement of IPM has experienced several significant stages. IPM is an ecosystem as a governance unit. It allows the presence of low numbers of pests because a direct or indirect relationship between biological species and their associated environmental living conditions could keep a long-term ecological balance. IPM also emphasizes overall benefits; it is necessary to integrate all kinds of safe, effective, economic, and simple managements as far as possible. When synthesizing and coordinating various managements, effective control object, effective time limit, effective scope, and effective degree of the individual method should be analysed first. The ultimate goal of IPM is for the sustainable development of agriculture. In the future IPM system engineering, crop resistance, the role of natural enemies, agricultural control, and the application of biological control technologies will be greatly strengthened, and the role of chemical pesticides will be significantly reduced. Safe, effective, and long-lasting control of pest populations will be below the level of harm caused, which could protect the ecological environment and the health of people and livestock, and promote the development of production.
文摘Proton therapy is the most advanced radiotherapy approach in the world,and causes less damage to normal human tissue than traditional radiotherapy.Because the treatment process produces a high-energy proton beam,the personnel safety interlock system mainly considers measures to protect personnel from radiation hazards during beam preparation and the beam release process.Unlike other safety interlock systems,the personnel safety interlock system designed in this study focuses on the safety and stability of the system itself.The hardware and software of important interlock control loops are designed and developed according to the requirements of Safety Integrity Level 3 specified by IEC61508.A set of redundant ring networks was developed to ensure that damage to a certain network line does not affect the normal operation of the system.A set of friendly operation interfaces and data storage systems were developed to ensure that the operator can monitor the data in real time and trace the data.The personnel safety interlock system mainly includes a beam enabling function,clearance function,and emergency stop function.The system was put into actual use and successfully ensured personnel safety.
基金supported by the 2020 Infrastructure Engineering Technology Innovation Projectthe“Intelligent Substation”Supporting Technology Research Project(031200WS22200001)。
文摘The reliability analysis of vertically integrated protection devices is crucial for designing International Electrotechnical Commission(IEC)61850-based substations.This paper presents the hardware architecture of a four-inone vertically integrated device and the information transmission path of each function based on the functional information transmission chain of protection devices,measurement and control devices,merging units,and intelligent terminals.Additionally,a reliability analysis model of the protection device and its protection system is constructed using the fault tree analysis method while considering the characteristics of each module of the vertically integrated device.The stability probability of the protection system in each state is analyzed by combining the state-transfer equations of line and busbar protection with a Markov chain.Finally,the failure rate and availability of the protection device and its protection system are calculated under different ambient temperatures using a 110 kV intelligent substation as an example.The sensitivity of each device module is analyzed.
文摘This paper presents an integrated protection technique for power distribution systems. A novel integrated protection scheme based on overcurrent protection technique for distribution system is described, in which a specially designed protection relay is installed at each substation of a network and responsible for the protection of every line sections connected to the substation busbar The conventional directional overcurrent and the new adaptive accelerated protection algorithms with multiple settings are implemented into the relay to cover all the protected line sections. The paper includes studies of a typical multi section distribution network to demonstrate the principle of the scheme. Studies show that the new scheme not only offer the new protection features for individual power line section, but also provide the characteristics of integrated protection.