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Interventional effect of hirudin on the expression of microtubule-associated protein 2 in peripheral tissue of hematom of model rats with acute intracerebral hemorrhage 被引量:2
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作者 Jiachun Feng Ying Zhang Fang Deng 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第3期230-233,共4页
BACKGROUND: It is suspected that dissociation, destruction or synthetic disorder of microtubule-associated protein 2 (MAP-2) may participate in secondary injury of intracerebral hemorrhage (ICH), and the reason m... BACKGROUND: It is suspected that dissociation, destruction or synthetic disorder of microtubule-associated protein 2 (MAP-2) may participate in secondary injury of intracerebral hemorrhage (ICH), and the reason may be related to thrombin in high concentration after ICH; therefore, the mechanism should be studied further. OBJECTIVE: To explore the effect of hirudin on expression of MAP-2 in peripheral tissue of hematom after ICH and changes of water content in brain tissue and analyze pathogenesis of thrombin in secondary injury after ICH. DESIGN : Completely randomized grouping design and controlled animal study SEn-ING : Department of Neurology, the First Affiliated Hospital of Jilin University MATERIALS : The experiment was carried out in the Neurological Laboratory of the First Affiliated Hospital of Jilin University from April 2003 to April 2004. A number of 80 healthy Wistar rats, of both genders, aged 3-4 months, weighing 250-350 g, were randomly divided into 8 groups: normal control group, 6-hour ICH group, 1-day ICH group, 2-day ICH group, 3-day ICH group, 7-day ICH group, 3-day hirudin group and 7-day hirudin group with 10 in each group. Five rats from each group were selected to measure their water content, and the others were undertaken immunohistochemical stain. Hirudin was produced by Sigma Company, USA, and MAP-2 rabbit-rat polyclonal antibody was provided by Fuzhou Maixin Biotechnology Company Limited. METHODS: ① Model establishing and grouping intervention: Rats in simple ICH group were collected their blood from tails and then inserted with 50 μL non-anticoagulant auto-arterial blood into the cauda of the putamen in right brain within 5 minutes. Rats in hirudin groups were inserted with 10 U hirudin (which was diluted with saline to 20 μL) into local hematom regions within 5 minutes, and the needle was pulled out after 10 minutes. Rats in normal control group were untouched. ② Water content in peripheral tissue of hematom: Based on the ratio between dry weight and wet weight, brain tissue at bleeding side and in right frontal lobe was selected to measure dry and wet weights so as to calculate the water content [(wet weight - dry weight) /wet weight] × 100%.③ Positive expression of MAP-2: Based on immunohistochemical stain, positive MAP-2 cells were regarded as neurons and they were buffy morphological. Positive rate of MAP-2 was calculated, i.e., percentage of positive cells in each sight to total cells in all sights. ④ Statistical analysis: Data among groups were compared with one-way analysis of variance, averages were compared with SNK-q test by each other, and relation between water content and MAP-2 was analyzed with linear regression technique. MAIN OUTCOME MEASURES: Changes of water content and MAP-2 expression in peripheral tissue of hematorn at various time points after ICH and intervention of hirudin. RESULTS: All 80 rats were involved in the final analysis. ①Water content: Water content was increased at day 1, reached peak at day 3 and decreased at day 7. It was (72.31±0.32)%, (77.42±0.53)%, (78.44±0.28)%, (74.10±0.13)%, (74.85±0.51)% and (70.07±0.36)%, respectively in 1-day, 2-day, 3-day and 7-day ICH groups and 3-day and 7-day hirudin groups, which was higher than that in normal control group (63.85±0.41, q=-4.684 3 to -7.262 0, P〈 0.05); that in 2-day and 3-day ICH groups was higher than that in 7-day ICH group (q=-3.053 4, -3.727 0, P 〈 0.05); and that in 3-day and 7-day ICH groups was higher than that in hirudin groups at the same time points (q=-2.965 6, -2.726 4, P 〈 0.05). ②Positive expression of MAP-2: Positive expression of MAP-2 was decreased at 6 hours after ICH, reached the lowest value at day 3 and increased at day 7. Positive rate was (78.60±0.42)%, (60.56±0.74)%, (44.60±0.26)%, (25.45±0.85)%, (32.55±0.64)%, (37.69+0.76)%, (41.75±0.68)%, respectively in 6-hour, 1-day, 2-day, 3-day and 7-day ICH groups and 3-day and 7-day hirudin groups, which was lower than that in normal control group [(96.50±0.33)%, q= -3.074 5 to -8.128 5, P 〈 0.05]. In addition, positive cells of MAP-2 disappeared plentifully at 3-7 days after ICH, stain of positive cells were light, and only stain of plasma was positive. That in 3-day and 7-day hirudin groups was higher than that in ICH groups at the same time points (q= -3.391 8, -2.967 9, P 〈 0.05). Moreover, positive cells of MAP-2 was formed slightly but deeply stained. ③ Results of linear regression: Water content was negatively related to MAP-2 changes at 7 days after ICH (r= -0.894 9, P〈 0.01), i.e., water content was increased with decrease of MAP-2 expression. CONCLUSION : The deterioration of MAP-2 may be involved in the pathogenesis of thrombin within the first week after ICH, and the local administration of hirudin can protect neurons. 展开更多
关键词 ICH interventional effect of hirudin on the expression of microtubule-associated protein 2 in peripheral tissue of hematom of model
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运用患者主观全面评定法对维持性血液透析患者蛋白能量消耗状态的调查及可干预危险因素分析 被引量:5
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作者 陈学勋 朱子豪 +9 位作者 刘志民 张成银 张海波 赵学兰 徐慧颖 李璇 曹波 柴春香 郭民 武文斌 《中国当代医药》 2020年第9期149-152,共4页
目的运用患者主观全面评定法(PG-SGA)对维持性血液透析(MHD)患者蛋白能量消耗(PEW)状态情况调查及可干预危险因素分析。方法选取2018年9~12月选取潍坊医学院附属医院、潍坊市益都中心医院、潍城经济开发区医院、潍坊市峡山生态经济开发... 目的运用患者主观全面评定法(PG-SGA)对维持性血液透析(MHD)患者蛋白能量消耗(PEW)状态情况调查及可干预危险因素分析。方法选取2018年9~12月选取潍坊医学院附属医院、潍坊市益都中心医院、潍城经济开发区医院、潍坊市峡山生态经济开发区人民医院四个透析中心共收治的432例MHD患者作为研究对象,采用横断面研究,运用PG-SGA结合问卷调查、人体测量、生化指标对MHD患者进行PEW状态评估及影响因素分析。结果432例MHD患者中PEW总发生率为52.78%。合并糖尿病、酸中毒、透析不充分Kt/V<1.2、年龄≥65岁、透析时间≥5年的患者PEW总发生率均分别高于非糖尿病、非酸中毒、透析充分、年龄<65岁、透析时间<5年的患者,差异有统计学意义(P<0.05)。体重指数(BMI)、上臂中点周径(MAC)、三头肌皮褶厚度(TSF)、上臂肌肉周径(MAMC)、清蛋白(ALB)、血红蛋白(Hb)各项指标在3个PG-SGA不同评分中的比较,PG-SGA C低于PG-SGA A和PG-SGA B,PG-SGA B低于PG-SGA A,差异均有统计学意义(P<0.05)。Logistic多因素分析显示,糖尿病(OR=3.01,95%CI:1.52~5.84)、酸中毒(OR=1.92,95%CI:1.13~3.34)、透析不充分Kt/V<1.2(OR=1.73,95%CI:1.05~3.68)均是导致MHD患者发生PEW的可干预危险因素(P<0.05)。结论MHD患者PEW发生率高,合并糖尿病患者尤甚;糖尿病、酸中毒、透析不充分Kt/V<1.2是导致MHD患者发生PEW的可干预危险因素。 展开更多
关键词 维持性血液透析 蛋白质能量消耗 患者主观全面评定法 可干预危险因素
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