Objective Keshan disease(KD)is a myocardial mitochondrial disease closely related to insufficient selenium(Se)and protein intake.PTEN induced putative kinase 1(PINK1)/Parkin mediated mitochondrial autophagy regulates ...Objective Keshan disease(KD)is a myocardial mitochondrial disease closely related to insufficient selenium(Se)and protein intake.PTEN induced putative kinase 1(PINK1)/Parkin mediated mitochondrial autophagy regulates various physiological and pathological processes in the body.This study aimed to elucidate the relationship between PINK1/Parkin-regulated mitochondrial autophagy and KD-related myocardial injury.Methods A low Se and low protein animal model was established.One hundred Wistar rats were randomly divided into 5 groups(control group,low Se group,low protein group,low Se+low protein group,and corn from KD area group).The JC-1 method was used to detect the mitochondrial membrane potential(MMP).ELISA was used to detect serum creatine kinase MB(CK-MB),cardiac troponin I(cTnI),and mitochondrial-glutamicoxalacetic transaminase(M-GOT)levels.RT-PCR and Western blot analysis were used to detect the expression of PINK1,Parkin,sequestome 1(P62),and microtubule-associated proteins1A/1B light chain 3B(MAP1LC3B).Results The MMP was significantly decreased and the activity of CK-MB,cTnI,and M-GOT significantly increased in each experimental group(low Se group,low protein group,low Se+low protein group and corn from KD area group)compared with the control group(P<0.05 for all).The mRNA and protein expression levels of PINK1,Parkin and MAP1LC3B were profoundly increased,and those of P62 markedly decreased in the experimental groups compared with the control group(P<0.05 for all).Conclusion Low Se and low protein levels exacerbate myocardial damage in KD by affecting the PINK1/Parkin-mediated mitochondrial autophagy pathway.展开更多
In the present experiments the changes in levels of ribosome,polysome and 3H-leucine incorporation rate in liver post-mitochondrial supernatant (PM-supernatant) were investigated in Sedericient and Se-supplented rats....In the present experiments the changes in levels of ribosome,polysome and 3H-leucine incorporation rate in liver post-mitochondrial supernatant (PM-supernatant) were investigated in Sedericient and Se-supplented rats.The results demonstrated that the amounts of ribosome and polysome as well as the ratio of polysome to ribosome in liver PM-supernatant from the Se-deficient rats were all remarkahly decreased.In the meantime,the rate of protein synthesis expressed as radioactivity or 3H-leucine incorporated into protein in the PM-supernatant system also decreased significantly.The results suggest that the decreases of ribosomes and proportion of ribosomal aggregates in PM-supernatant may be responsible for the decrease of the protein synthesis activity in liver of the Se-deficient animals.展开更多
Biochemical technique was used to separate three kinds of proteins (albumin, globulin and gliadin) in corn samples froin high selenium areas and normal areas in Erxi autonomous region of Hubei Province, China. The con...Biochemical technique was used to separate three kinds of proteins (albumin, globulin and gliadin) in corn samples froin high selenium areas and normal areas in Erxi autonomous region of Hubei Province, China. The contents of Se and other elements in these proteins were determined by neutron activation analysis (NAA). The results show that Se is enriched in corn proteins at high selenium area, while Cu, Al, Mn, V and Cl are also enriched in varying degrees.展开更多
【目的】研究硒对摄食高植物蛋白饲料大菱鲆(Scophthalmus maximus L.)幼鱼肝肠健康的影响,为降低低鱼粉饲料对鱼体肝肠健康的不利影响提供依据。【方法】以初始体质量(30.79±0.22)g的大菱鲆为研究对象,以鱼粉质量分数55%的饲料组...【目的】研究硒对摄食高植物蛋白饲料大菱鲆(Scophthalmus maximus L.)幼鱼肝肠健康的影响,为降低低鱼粉饲料对鱼体肝肠健康的不利影响提供依据。【方法】以初始体质量(30.79±0.22)g的大菱鲆为研究对象,以鱼粉质量分数55%的饲料组为正对照组(DP),以质量分数27.6%混合植物蛋白替代质量分数30%鱼粉为负对照组(DN),分别在低鱼粉中添加0.3(D1)、0.6(D2)、1.2(D3)、2.4(D4)、4.8(D5)mg/kg亚硒酸钠,以肝脏和肠道生理生化指标、肠道形态结构及肠道菌群结构等指标评价硒对大菱鲆幼鱼肝脏和肠道健康的影响。实验养殖周期52 d。【结果】1)与DP组相比,DN组显著降低大菱鲆肝脏和肠道总抗氧化能力(T-AOC)、总超氧化物歧化酶(SOD)和谷胱甘肽过氧化物(GSH-PX)酶活性(P<0.05);低鱼粉饲料中添加亚硒酸钠均可以不同程度提升上述所有指标,并降低丙二醛(MDA)含量(P<0.05)。2)与DP组相比,DN组显著提高了大菱鲆幼鱼肝脏肝糖原和脂肪酸合成酶(FAS)含量(P<0.05),而FAS含量则在D1-D5组显著低于DN组(P<0.05)。幼鱼肝脏SE-P含量在D2和D5组显著高于DN组(P<0.05)。3)0.3~1.2 mg/kg亚硒酸钠可抑制由低鱼粉引起的pat1、lzm和gst的mRNA表达水平的下调和前肠皱襞高度和黏膜厚度的降低,并可以显著提高肠道胰蛋白酶活性、脂肪酶活性和淀粉酶活性(P<0.05)。4)低鱼粉组肠道微生物组成的丰度和多样性显著降低,而亚硒酸钠组则可以明显改善这些不良现象,并可提升有益菌丰度,降低致病菌丰度。【结论】亚硒酸钠可缓解高植物蛋白饲料对大菱鲆造成的免疫下降、肠道组织结构破坏、消化功能下降及肠道菌群紊乱等不良影响。在低鱼粉饲料中亚硒酸钠的建议添加剂量为0.6~2.4 mg/kg。展开更多
基金supported by the Natural Science Foundation of Heilongjiang Province(No.LH2021H009).
文摘Objective Keshan disease(KD)is a myocardial mitochondrial disease closely related to insufficient selenium(Se)and protein intake.PTEN induced putative kinase 1(PINK1)/Parkin mediated mitochondrial autophagy regulates various physiological and pathological processes in the body.This study aimed to elucidate the relationship between PINK1/Parkin-regulated mitochondrial autophagy and KD-related myocardial injury.Methods A low Se and low protein animal model was established.One hundred Wistar rats were randomly divided into 5 groups(control group,low Se group,low protein group,low Se+low protein group,and corn from KD area group).The JC-1 method was used to detect the mitochondrial membrane potential(MMP).ELISA was used to detect serum creatine kinase MB(CK-MB),cardiac troponin I(cTnI),and mitochondrial-glutamicoxalacetic transaminase(M-GOT)levels.RT-PCR and Western blot analysis were used to detect the expression of PINK1,Parkin,sequestome 1(P62),and microtubule-associated proteins1A/1B light chain 3B(MAP1LC3B).Results The MMP was significantly decreased and the activity of CK-MB,cTnI,and M-GOT significantly increased in each experimental group(low Se group,low protein group,low Se+low protein group and corn from KD area group)compared with the control group(P<0.05 for all).The mRNA and protein expression levels of PINK1,Parkin and MAP1LC3B were profoundly increased,and those of P62 markedly decreased in the experimental groups compared with the control group(P<0.05 for all).Conclusion Low Se and low protein levels exacerbate myocardial damage in KD by affecting the PINK1/Parkin-mediated mitochondrial autophagy pathway.
文摘In the present experiments the changes in levels of ribosome,polysome and 3H-leucine incorporation rate in liver post-mitochondrial supernatant (PM-supernatant) were investigated in Sedericient and Se-supplented rats.The results demonstrated that the amounts of ribosome and polysome as well as the ratio of polysome to ribosome in liver PM-supernatant from the Se-deficient rats were all remarkahly decreased.In the meantime,the rate of protein synthesis expressed as radioactivity or 3H-leucine incorporated into protein in the PM-supernatant system also decreased significantly.The results suggest that the decreases of ribosomes and proportion of ribosomal aggregates in PM-supernatant may be responsible for the decrease of the protein synthesis activity in liver of the Se-deficient animals.
文摘Biochemical technique was used to separate three kinds of proteins (albumin, globulin and gliadin) in corn samples froin high selenium areas and normal areas in Erxi autonomous region of Hubei Province, China. The contents of Se and other elements in these proteins were determined by neutron activation analysis (NAA). The results show that Se is enriched in corn proteins at high selenium area, while Cu, Al, Mn, V and Cl are also enriched in varying degrees.
