Molecular imprinting of proteins remains a huge chal-lenge because of two major obstacles:difficulty in template removal and low imprinting efficiency.Herein,we propose a new strategy to simultaneously overcome these ...Molecular imprinting of proteins remains a huge chal-lenge because of two major obstacles:difficulty in template removal and low imprinting efficiency.Herein,we propose a new strategy to simultaneously overcome these two challenges by creating molecu-larly imprinted polymers(MIPs)with nanoscale shape-memorable imprint cavities.These novel MIPs were developed by simply cross-linking the polymers with a peptide cross-linker instead of commonly used cross-linkers.Due to the unique pH-induced helix–coil transition of the peptide cross-linker,adjusting the pH from 5.5 to 7.4 leads to an expansion of the imprint cavities,thus facilitating template removal.Returning the pH back to 5.5 restores the original size and shape of the imprint cavities due to the precise refolding of peptide.A template protein can there-fore be readily removed under mild conditions,while simultaneously achieving a significantly improved imprinting effect.展开更多
基金This work was funded by the National Natural Science Foundation of China(grant nos:51625302 and 51873091)the National Key Research and Development Program of China(2017YFC1103501).
文摘Molecular imprinting of proteins remains a huge chal-lenge because of two major obstacles:difficulty in template removal and low imprinting efficiency.Herein,we propose a new strategy to simultaneously overcome these two challenges by creating molecu-larly imprinted polymers(MIPs)with nanoscale shape-memorable imprint cavities.These novel MIPs were developed by simply cross-linking the polymers with a peptide cross-linker instead of commonly used cross-linkers.Due to the unique pH-induced helix–coil transition of the peptide cross-linker,adjusting the pH from 5.5 to 7.4 leads to an expansion of the imprint cavities,thus facilitating template removal.Returning the pH back to 5.5 restores the original size and shape of the imprint cavities due to the precise refolding of peptide.A template protein can there-fore be readily removed under mild conditions,while simultaneously achieving a significantly improved imprinting effect.
