BACKGROUND It is not uncommon to develop viral encephalitis.Epidemic Japanese B encephalitis infection combined with contactin-associated protein-like 2(CASPR-2)antibody-positive autoimmune encephalitis has not been r...BACKGROUND It is not uncommon to develop viral encephalitis.Epidemic Japanese B encephalitis infection combined with contactin-associated protein-like 2(CASPR-2)antibody-positive autoimmune encephalitis has not been reported at present.In clinical work,we need to consider more options.CASE SUMMARY A 32-year-old male worker presented with headache,fever and call-unresponsive presentation.Complete cranial magnetic resonance image showed symmetrical abnormal signals in bilateral medial temporal lobe,bilateral thalamus and basal ganglia.Improved lumbar puncture showed that cerebrospinal fluid protein and cell count increased significantly.Viral encephalitis was considered,and the patient's consciousness still increased rapidly after antiviral treatment.Further detection of Cerebrospinal fluid Japanese B encephalitis virus Polymerase Chain Reaction positive,serum autoimmune encephalitis antibody showed CASPR-2 antibody positive(1:320),the patient's condition gradually improved after plasma exchange treatment.3 mo later,the serum CASPR-2 antibody was negative and the patient's condition was stable.CONCLUSION This article reports the world’s first case of Epidemic Japanese B encephalitis infection combined with CASPR-2 antibody-positive autoimmune encephalitis,with a view to raising awareness.展开更多
SQUAMOSA promoter binding protein like(SPL)是一类在植物中广泛存在的转录因子家族,在调控植物生长发育、响应逆境胁迫等方面发挥着重要作用。为解析小麦中SPL家族基因响应非生物胁迫的机理,本研究采用生物信息学的方法在全基因组范...SQUAMOSA promoter binding protein like(SPL)是一类在植物中广泛存在的转录因子家族,在调控植物生长发育、响应逆境胁迫等方面发挥着重要作用。为解析小麦中SPL家族基因响应非生物胁迫的机理,本研究采用生物信息学的方法在全基因组范围内对小麦SPL基因家族成员进行鉴定,并对鉴定到的SPL基因进行表达模式分析。结果表明,在全基因组范围内共鉴定到56个小麦SPL基因,其中27个是miR156的靶基因;系统进化分析发现,56个小麦SPL基因聚类为7个亚家族。基于转录组数据对表达模式进行分析,发现36个小麦SPL基因与非生物胁迫响应相关,响应缺氮、缺磷、高盐、低温、干旱、高温胁迫以及热旱共胁迫的基因分别有12、16、22、6、13、14和21个,其中TraesCS3D02G425800同时响应7种非生物胁迫。qRT-PCR验证结果与转录组数据基本一致。展开更多
The conformational properties and elastic behaviors of protein-like single chains in the process of tensileelongation were investigated by means of Monte Carlo method.The sequences of protein-like single chains contai...The conformational properties and elastic behaviors of protein-like single chains in the process of tensileelongation were investigated by means of Monte Carlo method.The sequences of protein-like single chains contain two typesof residues:hydrophobic(H)and hydrophilic(P).The average conformations and thermodynamics statistical properties ofprotein-like single chains with various elongation ratio λ were calculated.It was found that the mean-square end-to-enddistance<R^2>(?).increases with elongation ratio λ.The tensor eigenvalues ratio of<L_2~2>:<L_1~2>decreases with elongationratio λ for short(HP)_x protein-like polymers,however,the ratio of<L_3~2>:<L_1~2>increases with elongation ratio λ,especially for long (H)_x sequence.Average energy per bond increases with elongation ratio λ,especially for (H)_xprotein-like single chains.Helmholtz free energy per bond also increases with elongation ratio λ.Elastic force(f),energycontribution to force(f_U)and entropy contribution to force(fs)for different protein-like single chains were also calculated.These investigations may provide some insights into elastic behaviors of proteins.展开更多
We study the translocation of a protein-like chain through a finite cylindrical channel using the pruned-enriched Rosenbluth method （PERM） and the modified orientation-dependent monomer-monomer interaction （ODI） m...We study the translocation of a protein-like chain through a finite cylindrical channel using the pruned-enriched Rosenbluth method （PERM） and the modified orientation-dependent monomer-monomer interaction （ODI） model. Attractive channels （εcp = -2.0, -1.0, -0.5）, repulsive chanaels （εcp： 0.5, 1.0, 2.0）, and a neutral channel （εcp =- 0） are discussed. The results of the chain dimension and the energy show that Z0 ： 1.0 is an important case to distinguish the types of the channels. For the strong attractive channel, more contacts form during the process of translocation. It is also found that an external force is needed to drive the chain outside of the channel with the strong attraction. While for the neutral, the repulsive, and the weak attractive channels, the translocation is spontaneous.展开更多
BACKGROUND Rearrangements of the anaplastic lymphoma kinase(ALK)gene(ALK-positive)represent an oncogenic driver in approximately 3%-5%of non-small-lung cancer(NSCLC)patients.Sarcoidosis is a multisystem disease,and it...BACKGROUND Rearrangements of the anaplastic lymphoma kinase(ALK)gene(ALK-positive)represent an oncogenic driver in approximately 3%-5%of non-small-lung cancer(NSCLC)patients.Sarcoidosis is a multisystem disease,and its reported incidence in Asia is 1 or less per 100000 people per year.The co-occurrence of sarcoidosis and ALK-positive NSCLC is rare,and ALK-positive lung cancer is likely to spread quickly.Therefore,the co-occurrence of sarcoidosis is more easily misdiagnosed as metastatic lung cancer by radiological examination.CASE SUMMARY A 50-year-old man had a nodule in the left superior lobe,many small nodules in left superior and right lungs,and enlarged bilateral hilar,mediastinal,and right supraclavicular lymph nodes.Computed tomography-guided pulmonary biopsy of the nodule in the left superior lobe revealed echinoderm microtubuleassociated protein-like 4 gene-ALK positive NSCLC with concomitant noncaseating granuloma.This patient was treated with crizotinib.Thirty days later,a chest computed tomography scan revealed a dramatic decrease in the size of the left superior lobe nodule;however,the lesions in the right lung progressed.The right supraclavicular lymph nodes showed granulomas,and no tumor cells were identified in the specimens. The angiotensin-converting enzyme level was high.After 1 wk of methylprednisolone treatment, a significant response of all lesionswas revealed. Following radical resection of the lung cancer, noncaseatinggranulomas were observed in both lung tissues and lymph nodes, which resultedin a diagnosis of echinoderm microtubule-associated protein-like 4-ALK positiveNSCLC accompanied with sarcoidosis.CONCLUSIONOur experience illustrates that pathological evidence is needed to confirmmetastatic disease, especially when some suspected metastatic lesions arenegative for malignancy.展开更多
实际地震波形观测表明,对于大陆结构相对简单的地壳中的地震而言,有一震相出现在P波和S波之间.一般在30～50km附近发育得较好,其能量主要集中在径向分量,而垂向分量的振幅相对径向要小,切向分量上的振幅很弱,且波形以低频为主,通常没有...实际地震波形观测表明,对于大陆结构相对简单的地壳中的地震而言,有一震相出现在P波和S波之间.一般在30～50km附近发育得较好,其能量主要集中在径向分量,而垂向分量的振幅相对径向要小,切向分量上的振幅很弱,且波形以低频为主,通常没有P波尖锐.在利用FK方法计算合成地震图的基础上,发现该震相是由S波入射到自由地表形成水平传播的P波(文献称为surface P wave,自由地表P波)或者包括S波入射到地表后形成的多次P波或其散射震相.由于该震相是由S波和P波之间耦合而形成,本文将其定义为sPL(s coupled into P)震相.理论波形研究表明,sPL相对直达P波的到时差对震中距离不敏感,而随着震源深度的增加几乎呈线性增加,因此可以很好的约束震源深度.本文以2005年江西九江地震为例,证实了sPL确定震源深度的可行性和可靠性.在观测到sPL震相的情况下,离震源50km以内的一个三分量地震台站的波形就可以帮助获得可靠的震源深度,而不需要精确的震中距离.由于sPL震相出现距离较近,对于较小(三级以上)的地震也可以应用,因此在稀疏台网布局情形下sPL对于确定中小地震深度应该具有很好的应用意义.展开更多
【目的】从枳[Poncirus trifoliata(L.)Raf.]中克隆SBP类[SQUAMOSA(SQUA)promoter-binding-like]转录因子基因SPL9和SPL13全长,构建SPL9和SPL13亚细胞定位表达载体验证其是否具有核定位功能,利用荧光定量PCR研究其在枳不同组织的表达特...【目的】从枳[Poncirus trifoliata(L.)Raf.]中克隆SBP类[SQUAMOSA(SQUA)promoter-binding-like]转录因子基因SPL9和SPL13全长,构建SPL9和SPL13亚细胞定位表达载体验证其是否具有核定位功能,利用荧光定量PCR研究其在枳不同组织的表达特性,初步确定SPL9和SPL13在枳生长发育过程中的作用。【方法】利用生物信息学结合RACE技术以枳花器官的cDNA为模板,克隆出SPL9和SPL13基因全长,分别命名Pt-SPL9和Pt-SPL13,大小分别是1519bp和1824bp,在GenBank的登录号分别是FJ502237和FJ502238;构建Pt-SPL9和Pt-SPL13亚细胞定位载体35S-GW-FJ502237/FJ502238-GFP,基因枪转化洋葱表皮细胞,暗培养24h后激光共聚焦显微镜下观察;利用SYBR Green I实时定量RT-PCR方法检测Pt-SPL9和Pt-SPL13在根、茎、叶、花序、花和果等不同组织中的表达。【结果】生物信息学分析表明,Pt-SPL9和Pt-SPL13的cDNA序列中都有microRNA156的识别位点,Pt-SPL9与金鱼草、拟南芥和玉米SPL9的同源性分别为48.9%、42.5%和41.7%;Pt-SPL13与拟南芥SPL13、水稻的SPL16和玉米的TGA1同源性分别为40.8%、38.1%和35.8%。Pt-SPL9和Pt-SPL13与其它植物的SBP一样有着高度保守的序列,即SBP结构域和一个双向核定位信号KRXXXRRRK。亚细胞定位结果表明,Pt-SPL9和Pt-SPL13均定位于细胞核中。SYBR Green I实时定量RT-PCR结果表明,Pt-SPL9和Pt-SPL13在各个器官均有表达,但表达量不同,Pt-SPL9在茎中的表达量最高,在花和叶中的表达量次之,在根、花芽和幼果中的表达量最低;Pt-SPL13在幼果中的表达量最高,在茎和花芽中的表达量相当,其次为叶,在花和根中的表达量很低。【结论】转录因子Pt-SPL9和Pt-SPL13均具有核定位功能,Pt-SPL9和Pt-SPL13对枳的茎和果实的发育可能有着重要作用。展开更多
【目的】梨二次成花(异常)在我国南方普遍发生,环境因子与植物遗传因子互作在该过程中的作用以及关键的遗传要素仍有待鉴定,为鉴定梨基因组中SPL(squamosa promoter-binding protein like)编码基因并研究其在二次成花诱导中的表达变化...【目的】梨二次成花(异常)在我国南方普遍发生,环境因子与植物遗传因子互作在该过程中的作用以及关键的遗传要素仍有待鉴定,为鉴定梨基因组中SPL(squamosa promoter-binding protein like)编码基因并研究其在二次成花诱导中的表达变化情况。【方法】前期课题组探索了通过摘叶诱导模拟二次成花的生理机制,测定了转录组变化过程,本研究基于已有的白梨(Pyrus bretschneideri Rehd.)基因组数据,从全基因组范围对SPL编码基因进行了鉴定,结合二次花诱导过程的转录组数据,挖掘参与该过程的候选基因。【结果】本研究共获得了34个典型的SPL蛋白家族成员,只有3个成员在目前的梨参考基因组上串联排列。系统进化分析将34个成员划归为8个进化类群,与其他物种SPL分类一致。砂梨‘丰水’二次成花诱导及未诱导材料的转录谱分析表明,34个SPL蛋白编码基因中仅两个显著差异表达,其中Pbr038289.1显著上调表达,而Pbr002303.1表达量显著下降。【结论】这2个蛋白有望成为深入研究梨成花过程调控的重要靶点。展开更多
文摘BACKGROUND It is not uncommon to develop viral encephalitis.Epidemic Japanese B encephalitis infection combined with contactin-associated protein-like 2(CASPR-2)antibody-positive autoimmune encephalitis has not been reported at present.In clinical work,we need to consider more options.CASE SUMMARY A 32-year-old male worker presented with headache,fever and call-unresponsive presentation.Complete cranial magnetic resonance image showed symmetrical abnormal signals in bilateral medial temporal lobe,bilateral thalamus and basal ganglia.Improved lumbar puncture showed that cerebrospinal fluid protein and cell count increased significantly.Viral encephalitis was considered,and the patient's consciousness still increased rapidly after antiviral treatment.Further detection of Cerebrospinal fluid Japanese B encephalitis virus Polymerase Chain Reaction positive,serum autoimmune encephalitis antibody showed CASPR-2 antibody positive(1:320),the patient's condition gradually improved after plasma exchange treatment.3 mo later,the serum CASPR-2 antibody was negative and the patient's condition was stable.CONCLUSION This article reports the world’s first case of Epidemic Japanese B encephalitis infection combined with CASPR-2 antibody-positive autoimmune encephalitis,with a view to raising awareness.
文摘SQUAMOSA promoter binding protein like(SPL)是一类在植物中广泛存在的转录因子家族,在调控植物生长发育、响应逆境胁迫等方面发挥着重要作用。为解析小麦中SPL家族基因响应非生物胁迫的机理,本研究采用生物信息学的方法在全基因组范围内对小麦SPL基因家族成员进行鉴定,并对鉴定到的SPL基因进行表达模式分析。结果表明,在全基因组范围内共鉴定到56个小麦SPL基因,其中27个是miR156的靶基因;系统进化分析发现,56个小麦SPL基因聚类为7个亚家族。基于转录组数据对表达模式进行分析,发现36个小麦SPL基因与非生物胁迫响应相关,响应缺氮、缺磷、高盐、低温、干旱、高温胁迫以及热旱共胁迫的基因分别有12、16、22、6、13、14和21个,其中TraesCS3D02G425800同时响应7种非生物胁迫。qRT-PCR验证结果与转录组数据基本一致。
基金The research was financially supported by the National Natural Science Foundation of China(Nos.20174036,20274040)the Natural Science Foundation of Zhejiang Province(No.R404047).
文摘The conformational properties and elastic behaviors of protein-like single chains in the process of tensileelongation were investigated by means of Monte Carlo method.The sequences of protein-like single chains contain two typesof residues:hydrophobic(H)and hydrophilic(P).The average conformations and thermodynamics statistical properties ofprotein-like single chains with various elongation ratio λ were calculated.It was found that the mean-square end-to-enddistance<R^2>(?).increases with elongation ratio λ.The tensor eigenvalues ratio of<L_2~2>:<L_1~2>decreases with elongationratio λ for short(HP)_x protein-like polymers,however,the ratio of<L_3~2>:<L_1~2>increases with elongation ratio λ,especially for long (H)_x sequence.Average energy per bond increases with elongation ratio λ,especially for (H)_xprotein-like single chains.Helmholtz free energy per bond also increases with elongation ratio λ.Elastic force(f),energycontribution to force(f_U)and entropy contribution to force(fs)for different protein-like single chains were also calculated.These investigations may provide some insights into elastic behaviors of proteins.
基金Project supported by the National Natural Science Foundation of China （Grant No. 20904047）, the Science and Technology Planning Project of Zhejiang Province, China （Grant No. 20100022）, and the Natural Science Foundation of Zhejiang Province, China （Grant No. Y6110304）.
文摘We study the translocation of a protein-like chain through a finite cylindrical channel using the pruned-enriched Rosenbluth method （PERM） and the modified orientation-dependent monomer-monomer interaction （ODI） model. Attractive channels （εcp = -2.0, -1.0, -0.5）, repulsive chanaels （εcp： 0.5, 1.0, 2.0）, and a neutral channel （εcp =- 0） are discussed. The results of the chain dimension and the energy show that Z0 ： 1.0 is an important case to distinguish the types of the channels. For the strong attractive channel, more contacts form during the process of translocation. It is also found that an external force is needed to drive the chain outside of the channel with the strong attraction. While for the neutral, the repulsive, and the weak attractive channels, the translocation is spontaneous.
基金The Zhejiang Provincial Natural Science Foundation of China,No.LQ17H160005.
文摘BACKGROUND Rearrangements of the anaplastic lymphoma kinase(ALK)gene(ALK-positive)represent an oncogenic driver in approximately 3%-5%of non-small-lung cancer(NSCLC)patients.Sarcoidosis is a multisystem disease,and its reported incidence in Asia is 1 or less per 100000 people per year.The co-occurrence of sarcoidosis and ALK-positive NSCLC is rare,and ALK-positive lung cancer is likely to spread quickly.Therefore,the co-occurrence of sarcoidosis is more easily misdiagnosed as metastatic lung cancer by radiological examination.CASE SUMMARY A 50-year-old man had a nodule in the left superior lobe,many small nodules in left superior and right lungs,and enlarged bilateral hilar,mediastinal,and right supraclavicular lymph nodes.Computed tomography-guided pulmonary biopsy of the nodule in the left superior lobe revealed echinoderm microtubuleassociated protein-like 4 gene-ALK positive NSCLC with concomitant noncaseating granuloma.This patient was treated with crizotinib.Thirty days later,a chest computed tomography scan revealed a dramatic decrease in the size of the left superior lobe nodule;however,the lesions in the right lung progressed.The right supraclavicular lymph nodes showed granulomas,and no tumor cells were identified in the specimens. The angiotensin-converting enzyme level was high.After 1 wk of methylprednisolone treatment, a significant response of all lesionswas revealed. Following radical resection of the lung cancer, noncaseatinggranulomas were observed in both lung tissues and lymph nodes, which resultedin a diagnosis of echinoderm microtubule-associated protein-like 4-ALK positiveNSCLC accompanied with sarcoidosis.CONCLUSIONOur experience illustrates that pathological evidence is needed to confirmmetastatic disease, especially when some suspected metastatic lesions arenegative for malignancy.
文摘实际地震波形观测表明,对于大陆结构相对简单的地壳中的地震而言,有一震相出现在P波和S波之间.一般在30～50km附近发育得较好,其能量主要集中在径向分量,而垂向分量的振幅相对径向要小,切向分量上的振幅很弱,且波形以低频为主,通常没有P波尖锐.在利用FK方法计算合成地震图的基础上,发现该震相是由S波入射到自由地表形成水平传播的P波(文献称为surface P wave,自由地表P波)或者包括S波入射到地表后形成的多次P波或其散射震相.由于该震相是由S波和P波之间耦合而形成,本文将其定义为sPL(s coupled into P)震相.理论波形研究表明,sPL相对直达P波的到时差对震中距离不敏感,而随着震源深度的增加几乎呈线性增加,因此可以很好的约束震源深度.本文以2005年江西九江地震为例,证实了sPL确定震源深度的可行性和可靠性.在观测到sPL震相的情况下,离震源50km以内的一个三分量地震台站的波形就可以帮助获得可靠的震源深度,而不需要精确的震中距离.由于sPL震相出现距离较近,对于较小(三级以上)的地震也可以应用,因此在稀疏台网布局情形下sPL对于确定中小地震深度应该具有很好的应用意义.
文摘【目的】从枳[Poncirus trifoliata(L.)Raf.]中克隆SBP类[SQUAMOSA(SQUA)promoter-binding-like]转录因子基因SPL9和SPL13全长,构建SPL9和SPL13亚细胞定位表达载体验证其是否具有核定位功能,利用荧光定量PCR研究其在枳不同组织的表达特性,初步确定SPL9和SPL13在枳生长发育过程中的作用。【方法】利用生物信息学结合RACE技术以枳花器官的cDNA为模板,克隆出SPL9和SPL13基因全长,分别命名Pt-SPL9和Pt-SPL13,大小分别是1519bp和1824bp,在GenBank的登录号分别是FJ502237和FJ502238;构建Pt-SPL9和Pt-SPL13亚细胞定位载体35S-GW-FJ502237/FJ502238-GFP,基因枪转化洋葱表皮细胞,暗培养24h后激光共聚焦显微镜下观察;利用SYBR Green I实时定量RT-PCR方法检测Pt-SPL9和Pt-SPL13在根、茎、叶、花序、花和果等不同组织中的表达。【结果】生物信息学分析表明,Pt-SPL9和Pt-SPL13的cDNA序列中都有microRNA156的识别位点,Pt-SPL9与金鱼草、拟南芥和玉米SPL9的同源性分别为48.9%、42.5%和41.7%;Pt-SPL13与拟南芥SPL13、水稻的SPL16和玉米的TGA1同源性分别为40.8%、38.1%和35.8%。Pt-SPL9和Pt-SPL13与其它植物的SBP一样有着高度保守的序列,即SBP结构域和一个双向核定位信号KRXXXRRRK。亚细胞定位结果表明,Pt-SPL9和Pt-SPL13均定位于细胞核中。SYBR Green I实时定量RT-PCR结果表明,Pt-SPL9和Pt-SPL13在各个器官均有表达,但表达量不同,Pt-SPL9在茎中的表达量最高,在花和叶中的表达量次之,在根、花芽和幼果中的表达量最低;Pt-SPL13在幼果中的表达量最高,在茎和花芽中的表达量相当,其次为叶,在花和根中的表达量很低。【结论】转录因子Pt-SPL9和Pt-SPL13均具有核定位功能,Pt-SPL9和Pt-SPL13对枳的茎和果实的发育可能有着重要作用。
