A series of double-hydrophilic double-grafted PMA-g-PEG/PDMA copolymers, which contained poly（methacrylate） （PMA） as backbone, poly（ethylene glycol） （PEG） and poly（N,N-dimethylacrylamide） （PDMA） as side ch...A series of double-hydrophilic double-grafted PMA-g-PEG/PDMA copolymers, which contained poly（methacrylate） （PMA） as backbone, poly（ethylene glycol） （PEG） and poly（N,N-dimethylacrylamide） （PDMA） as side chains synthesized successfully by using reversible addition-fragmentation chain transfer （RAFT） polymerization and atom transfer radical polymerization （ATRP）, were used as physical coatings for the evaluation of protein-resistant properties by capillary electrophoresis （CE）. Electroosmotic flow （EOF） measurement results showed that the PMA-g-PEG/PDMA copolymer coated capillaries could suppress electroosmotic mobility in a wide pH range （pH = 2.8-9.8） and EOF mobility decreased with the increase of copolymer molecular mass and PDMA content. At the same time, protein recovery, theoretical plate number of separation and repeatability of migration time demonstrated that antifouling efficiency was improved with the increase of molecular mass and PEG content.展开更多
A novel polydopamine-graft-poly（2-methyl-2-oxazoline） （PDA-g-PMOXA） coating was prepared by immobilizing poly（2-methyl-2-oxazoline） （PMOXA） onto material surfaces through polydopamine （PDA） anchored coating ...A novel polydopamine-graft-poly（2-methyl-2-oxazoline） （PDA-g-PMOXA） coating was prepared by immobilizing poly（2-methyl-2-oxazoline） （PMOXA） onto material surfaces through polydopamine （PDA） anchored coating for the first time. And then, the chemical composition, hydrophilicity, and protein-resistant properties of the PDA-g-PMOXA coating were studied using X-ray photoelectron spectroscopy （XPS）, contact angel （CA） test, surface plasmon resonance （SPR）, and quartz crystal microbalance with dissipation （QCM-D） measurement. Finally, the coating was applied to the capillary inner surface for protein separation by capillary electrophoresis （CE）.展开更多
Hydrophilic silica/copolymer nanoparticles of SiO<sub>2</sub>-g-P(PEGMA)-b-P(PEG) are prepared by silica surface-initiating atom transfer radical polymerization (SI-ATRP) of poly (ethylene glycol) methyl e...Hydrophilic silica/copolymer nanoparticles of SiO<sub>2</sub>-g-P(PEGMA)-b-P(PEG) are prepared by silica surface-initiating atom transfer radical polymerization (SI-ATRP) of poly (ethylene glycol) methyl ether methacrylate (PEGMA) and poly(ethylene glycol) methacrylate (PEG), by using Three molar ratios of SiO<sub>2</sub>-Br/PEGMA/PEG as 1/42.46/19.44, 1/42.46/38.88 and 1/42.46/77.76. Their temperature sensitive behaviour, pH response and surface properties as protein-resistance coatings are characterized. 220 nm core-shell nanoparticles as P(PEGMA)-b-P(PEG) shell grafted on SiO2 core are formed in water solution, which gained LCST at 60<sup>。</sup>C - 77<sup>。</sup>C and good dispersion in water when pH > 5.0. The water-casted films by SiO<sub>2</sub>-g-P(PEGMA)-b-P(PEG) obtain a little rough surface (Ra = 26.8 - 29.7 nm). While, the introduction of P(PEG) segments could slight increase the protein-repelling adsorption of SiO<sub>2</sub>-g-P(PEGMA)-b-P(PEG) films (△f = ?6.96 Hz ~ ?7.25 Hz) compared with SiO2-g-P(PEGMA) films (△f = ?9.5 Hz). Therefore, SiO2-g-P(PEGMA)-b-P(PEG) could be used as protein-resistance coatings.展开更多
Functionalization of silicon substrate surfaces with a stable monolayer for resisting non-specific adsorption of proteins has attracted great interest,since it is directly relevant to the development of miniature,sili...Functionalization of silicon substrate surfaces with a stable monolayer for resisting non-specific adsorption of proteins has attracted great interest,since it is directly relevant to the development of miniature,silicon-based biosensors and implantable microdevices,such as silicon-neuron interfaces.This brief review summarizes our contribution to the development of robust monolayers grown by surface hydrosilylation on atomically flat,hydrogen-terminated silicon surfaces.The review also outlines our strategy and progress on the fabrication of single molecule patterns on such monolayer platforms.展开更多
基金supported by the National Natural Science Foundation of China (No. 21074124)Ministry of Science and Technology of China (No. 2012CB933802)
文摘A series of double-hydrophilic double-grafted PMA-g-PEG/PDMA copolymers, which contained poly（methacrylate） （PMA） as backbone, poly（ethylene glycol） （PEG） and poly（N,N-dimethylacrylamide） （PDMA） as side chains synthesized successfully by using reversible addition-fragmentation chain transfer （RAFT） polymerization and atom transfer radical polymerization （ATRP）, were used as physical coatings for the evaluation of protein-resistant properties by capillary electrophoresis （CE）. Electroosmotic flow （EOF） measurement results showed that the PMA-g-PEG/PDMA copolymer coated capillaries could suppress electroosmotic mobility in a wide pH range （pH = 2.8-9.8） and EOF mobility decreased with the increase of copolymer molecular mass and PDMA content. At the same time, protein recovery, theoretical plate number of separation and repeatability of migration time demonstrated that antifouling efficiency was improved with the increase of molecular mass and PEG content.
基金support of the Ministry of Science and Technology of China(No.2012CB933802)
文摘A novel polydopamine-graft-poly（2-methyl-2-oxazoline） （PDA-g-PMOXA） coating was prepared by immobilizing poly（2-methyl-2-oxazoline） （PMOXA） onto material surfaces through polydopamine （PDA） anchored coating for the first time. And then, the chemical composition, hydrophilicity, and protein-resistant properties of the PDA-g-PMOXA coating were studied using X-ray photoelectron spectroscopy （XPS）, contact angel （CA） test, surface plasmon resonance （SPR）, and quartz crystal microbalance with dissipation （QCM-D） measurement. Finally, the coating was applied to the capillary inner surface for protein separation by capillary electrophoresis （CE）.
文摘Hydrophilic silica/copolymer nanoparticles of SiO<sub>2</sub>-g-P(PEGMA)-b-P(PEG) are prepared by silica surface-initiating atom transfer radical polymerization (SI-ATRP) of poly (ethylene glycol) methyl ether methacrylate (PEGMA) and poly(ethylene glycol) methacrylate (PEG), by using Three molar ratios of SiO<sub>2</sub>-Br/PEGMA/PEG as 1/42.46/19.44, 1/42.46/38.88 and 1/42.46/77.76. Their temperature sensitive behaviour, pH response and surface properties as protein-resistance coatings are characterized. 220 nm core-shell nanoparticles as P(PEGMA)-b-P(PEG) shell grafted on SiO2 core are formed in water solution, which gained LCST at 60<sup>。</sup>C - 77<sup>。</sup>C and good dispersion in water when pH > 5.0. The water-casted films by SiO<sub>2</sub>-g-P(PEGMA)-b-P(PEG) obtain a little rough surface (Ra = 26.8 - 29.7 nm). While, the introduction of P(PEG) segments could slight increase the protein-repelling adsorption of SiO<sub>2</sub>-g-P(PEGMA)-b-P(PEG) films (△f = ?6.96 Hz ~ ?7.25 Hz) compared with SiO2-g-P(PEGMA) films (△f = ?9.5 Hz). Therefore, SiO2-g-P(PEGMA)-b-P(PEG) could be used as protein-resistance coatings.
基金supported by the Welch Foundation grant E-1498NSF CAREER Award (CTS-0349228 to CC)+1 种基金grant DMR-0706627NIH R21 HD058985
文摘Functionalization of silicon substrate surfaces with a stable monolayer for resisting non-specific adsorption of proteins has attracted great interest,since it is directly relevant to the development of miniature,silicon-based biosensors and implantable microdevices,such as silicon-neuron interfaces.This brief review summarizes our contribution to the development of robust monolayers grown by surface hydrosilylation on atomically flat,hydrogen-terminated silicon surfaces.The review also outlines our strategy and progress on the fabrication of single molecule patterns on such monolayer platforms.
