PCR amplification and sequencing of whole blood DNA from an individual with hereditary spastic paraplegia, as well as family members, revealed a fragment of proteolipid protein 1 （PLP1） gene exon 1, which excluded t...PCR amplification and sequencing of whole blood DNA from an individual with hereditary spastic paraplegia, as well as family members, revealed a fragment of proteolipid protein 1 （PLP1） gene exon 1, which excluded the possibility of isomer 1 expression for this family. The fragment sequence of exon 3 and exon 5 was consistent with the proteolipid protein 1 sequence at NCBI. In the proband samples, a PLP1 point mutation in exon 4 was detected at the basic group of position 844, T→C, phenylalanine→leucine. In proband samples from a male cousin, the basic group at position 844 was C, but gene sequencing signals revealed mixed signals of T and C, indicating possible mutation at this locus. Results demonstrated that changes in PLP1 exon 4 amino acids were associated with onset of hereditary spastic paraplegia.展开更多
Schizophrenia(SCZ)is a serious mental illness whose etiology and pathogenesis are not yet clear.The level of miRNA may be a crucial factor in the occurrence and development of SCZ.This study found that miR-495 may reg...Schizophrenia(SCZ)is a serious mental illness whose etiology and pathogenesis are not yet clear.The level of miRNA may be a crucial factor in the occurrence and development of SCZ.This study found that miR-495 may regulate the susceptibility gene of SCZ and that proteolipid protein 1(PLP1)as a risk gene for schizophrenia may be involved in its pathogenesis.In this article we review the research progress related to hsa-miR-495-3p(miR-495),PLP1,and schizophrenia.展开更多
Schizophrenia (SCZ) is a serious mental illness with unknown etiology, high recurrence rate and high disability rate, which has caused a great burden to individuals and society. There is no clear etiology and pathogen...Schizophrenia (SCZ) is a serious mental illness with unknown etiology, high recurrence rate and high disability rate, which has caused a great burden to individuals and society. There is no clear etiology and pathogenesis. Methylation of N6-methyladenosine (m6A) can regulate the nervous and mental system, and affect the function of the nervous system. Proteolipid protein 1 (PLP1) is a risk gene for schizophrenia. In this study we review the research progress on the pathogenesis of schizophrenia, m6A methylation, and PLP1 gene.展开更多
Background Pelizaeus-Merzbacher disease （PMD） is a rare X-linked recessive disorder with symptoms including nystagmus, impaired motor development, ataxia, and progressive spasticity. The proteolipidprotein 1 （PLP1...Background Pelizaeus-Merzbacher disease （PMD） is a rare X-linked recessive disorder with symptoms including nystagmus, impaired motor development, ataxia, and progressive spasticity. The proteolipidprotein 1 （PLP1） gene is the only pathogenic gene of PMD. Duplication of the PLP1 gene is the most frequent gene defect, accounting for 50%-70% of PMD cases, whereas point mutations in the coding sequence or the splice sites account for 10%-25% of PMD cases. This study aimed to identify PLP1 mutations in nine unrelated Chinese patients （P1-9） with PMD, and 14 subjects from the family of patient 2 were also described. Methods Genomic DNA was extracted from peripheral multiplex ligation-dependent probe amplification （MLPA）. AI amplified and analyzed using direct DNA sequencing. blood samples. Gene dosage was determined using the 7 exons and exon-intron boundaries of the PLP1 gene were Results Of these nine patients, there were four transitional, four classical, and one connatal PMD according to their clinical and radiological presentations. PLP1 duplications were identified in patients 1-7 with PMD. Their mothers were PLPI duplications carriers as well. Both duplication carriers and normal genotypes of PLP1 were identified in the family members of patient 2. A c.517C〉T （p. P173S） hemizygous missense mutation in exon 4 was found in patient 8 with PMD, and his mother was shown to be a heterozygote of this mutation. Conclusions We identified seven genomic duplications and one missense mutation （p. P173S） of the PLP1 gene in eight Chinese patients with PMD. This is the report about PLP1 mutations in PMD patients from the mainland of China.展开更多
To detect the function of proteolipid protein (PLP) peptide (residues 56-70) sp ecific CD 4 + T cells in experimental allergic encephalomyelitis (EAE) in Bioz zi AB/H mice (H 2A g7 ) Methods Biozzi AB/H mice were immu...To detect the function of proteolipid protein (PLP) peptide (residues 56-70) sp ecific CD 4 + T cells in experimental allergic encephalomyelitis (EAE) in Bioz zi AB/H mice (H 2A g7 ) Methods Biozzi AB/H mice were immunized by synthetic PLP 56 70 peptide (DYEYLINVIH AFQYV) which was emulsified by sonication with complete Freund’s adjuvant, a EAE model proven histologically and clinically Murine splenocytes and spinal cord infiltrated (SCI) T cells were stimulated by PLP 56 70 , then the CD 4 + T cells were isolated by Dynabeads, and confirmed by staining with anti CD 4 antibody Finally, the IL2 bioassay and IFN γ/IL4 ELISA were done to detect T cell proliferation and cytokine secretion after PLP 56 70 stimulation Results The histology of murine spinal cord showed a great number of lymphocytes infiltr ated the spinal cord; the clinical signs showed high scores (4 3) on the peak, as well as a good EAE model After being isolated by Dynabea ds , CD 4 + T cells showed high purification (>99%) by staining with anti CD 4 antibody IL2 bioassay showed that those T cells were PLP 56 70 specific T cells ELISA showed that those T cells had high IFN γ/IL4 ratio , indicating that they are T helper 1 (Th1) cells Conclusions PLP 56 70 specific splenocytes and SCI CD 4 + T cells in EAE from Bioz zi AB/H mice were detected and showed that both of them were P LP 56 70 specific Th1 cells It is beneficial to understand what kind o f role these T cells play in the development of展开更多
文摘PCR amplification and sequencing of whole blood DNA from an individual with hereditary spastic paraplegia, as well as family members, revealed a fragment of proteolipid protein 1 （PLP1） gene exon 1, which excluded the possibility of isomer 1 expression for this family. The fragment sequence of exon 3 and exon 5 was consistent with the proteolipid protein 1 sequence at NCBI. In the proband samples, a PLP1 point mutation in exon 4 was detected at the basic group of position 844, T→C, phenylalanine→leucine. In proband samples from a male cousin, the basic group at position 844 was C, but gene sequencing signals revealed mixed signals of T and C, indicating possible mutation at this locus. Results demonstrated that changes in PLP1 exon 4 amino acids were associated with onset of hereditary spastic paraplegia.
基金Yunnan Provincial Department of Science and Technology Project(202101AY070001-224).
文摘Schizophrenia(SCZ)is a serious mental illness whose etiology and pathogenesis are not yet clear.The level of miRNA may be a crucial factor in the occurrence and development of SCZ.This study found that miR-495 may regulate the susceptibility gene of SCZ and that proteolipid protein 1(PLP1)as a risk gene for schizophrenia may be involved in its pathogenesis.In this article we review the research progress related to hsa-miR-495-3p(miR-495),PLP1,and schizophrenia.
基金Yunnan Provincial Department of Science and Technology Project(202101AY070001-224).
文摘Schizophrenia (SCZ) is a serious mental illness with unknown etiology, high recurrence rate and high disability rate, which has caused a great burden to individuals and society. There is no clear etiology and pathogenesis. Methylation of N6-methyladenosine (m6A) can regulate the nervous and mental system, and affect the function of the nervous system. Proteolipid protein 1 (PLP1) is a risk gene for schizophrenia. In this study we review the research progress on the pathogenesis of schizophrenia, m6A methylation, and PLP1 gene.
文摘Background Pelizaeus-Merzbacher disease （PMD） is a rare X-linked recessive disorder with symptoms including nystagmus, impaired motor development, ataxia, and progressive spasticity. The proteolipidprotein 1 （PLP1） gene is the only pathogenic gene of PMD. Duplication of the PLP1 gene is the most frequent gene defect, accounting for 50%-70% of PMD cases, whereas point mutations in the coding sequence or the splice sites account for 10%-25% of PMD cases. This study aimed to identify PLP1 mutations in nine unrelated Chinese patients （P1-9） with PMD, and 14 subjects from the family of patient 2 were also described. Methods Genomic DNA was extracted from peripheral multiplex ligation-dependent probe amplification （MLPA）. AI amplified and analyzed using direct DNA sequencing. blood samples. Gene dosage was determined using the 7 exons and exon-intron boundaries of the PLP1 gene were Results Of these nine patients, there were four transitional, four classical, and one connatal PMD according to their clinical and radiological presentations. PLP1 duplications were identified in patients 1-7 with PMD. Their mothers were PLPI duplications carriers as well. Both duplication carriers and normal genotypes of PLP1 were identified in the family members of patient 2. A c.517C〉T （p. P173S） hemizygous missense mutation in exon 4 was found in patient 8 with PMD, and his mother was shown to be a heterozygote of this mutation. Conclusions We identified seven genomic duplications and one missense mutation （p. P173S） of the PLP1 gene in eight Chinese patients with PMD. This is the report about PLP1 mutations in PMD patients from the mainland of China.
文摘To detect the function of proteolipid protein (PLP) peptide (residues 56-70) sp ecific CD 4 + T cells in experimental allergic encephalomyelitis (EAE) in Bioz zi AB/H mice (H 2A g7 ) Methods Biozzi AB/H mice were immunized by synthetic PLP 56 70 peptide (DYEYLINVIH AFQYV) which was emulsified by sonication with complete Freund’s adjuvant, a EAE model proven histologically and clinically Murine splenocytes and spinal cord infiltrated (SCI) T cells were stimulated by PLP 56 70 , then the CD 4 + T cells were isolated by Dynabeads, and confirmed by staining with anti CD 4 antibody Finally, the IL2 bioassay and IFN γ/IL4 ELISA were done to detect T cell proliferation and cytokine secretion after PLP 56 70 stimulation Results The histology of murine spinal cord showed a great number of lymphocytes infiltr ated the spinal cord; the clinical signs showed high scores (4 3) on the peak, as well as a good EAE model After being isolated by Dynabea ds , CD 4 + T cells showed high purification (>99%) by staining with anti CD 4 antibody IL2 bioassay showed that those T cells were PLP 56 70 specific T cells ELISA showed that those T cells had high IFN γ/IL4 ratio , indicating that they are T helper 1 (Th1) cells Conclusions PLP 56 70 specific splenocytes and SCI CD 4 + T cells in EAE from Bioz zi AB/H mice were detected and showed that both of them were P LP 56 70 specific Th1 cells It is beneficial to understand what kind o f role these T cells play in the development of
