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Expression of c-erbB-2 oncogene protein, epidermal growth factor receptor, and TGF-β1 in human pancreatic ductal adenocarcinoma 被引量:1
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《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2002年第4期620-623,共4页
Objective: To detect the relations of c-erbB-2 onco-gene protein, epidermal growth factor receptor (EG-FR) and transforming growth factor-β1 (TGF-β1)to the progression or metastasis of pancreatic carci-noma.Methods:... Objective: To detect the relations of c-erbB-2 onco-gene protein, epidermal growth factor receptor (EG-FR) and transforming growth factor-β1 (TGF-β1)to the progression or metastasis of pancreatic carci-noma.Methods: Using streptavidinbiotin complex (SABC)method, c-erbB-2 oncongene protein, we examinedimmunohistochemically EGFR and TGF-β1 expres-sions in wax-tissue sections from 10 individuals withnormal pancreas (NP), 13 patients with chronic pan-creatitis (CP) and 36 patients with pancreatic ductaladenocarcinoma (PC).Results: The positive expression rates of c-cerbB-2oncogene protein, EGFR and TGF-β1 in the NP, CPand PC groups were 0, 0, 10%; 7.7%, 7.7%,7.7%; and 41.7%, 50.0%, 44.4%, respectively.The positive expression rates of the three specific pro-teins increased more significantly in the PC groupthan in the NP and CP groups (P【0.05). The indi-vidual expression of c-erbB-2, EGFR and TGF-β1was not related to the age and sex of the patients aswell as the site, size and histopathological grade oftumors (P】0.05), but to the clinical stage of tumors(P【0.01). The coexpression rate of the three pro-teins was 27.8 % (10/36). This coexpression in thePC group was correlated with the histopathologicalgrades and clinical stages of tumors (P【0.01).Conclusion: Detection of c-erbB-2 oncogene protein,EGFR, and TGF-β1 expressions in pancreatic tissueis helpful to judge the malignancy, progression, andmetastasis of PC. 展开更多
关键词 pancreatic neoplasms proto-oncogene proteins c-erbB-2/AN receptors EPIDERMAL GROWTH FACTOR receptor transforming GROWTH factor-β1
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Decline in the expression of IL-2 after trauma and changes in the nuclear transcription factors NFAT and AP-1 被引量:1
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作者 罗艳 梁华平 +2 位作者 胡承香 徐祥 王正国 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第9期1348-1351,150,共4页
OBJECTIVE: To investigate whether the decrease in expression of interleukin-2 (IL-2) after trauma is associated with changes in DNA binding activity of nuclear factor of activated T cells (NFAT) and activator protein-... OBJECTIVE: To investigate whether the decrease in expression of interleukin-2 (IL-2) after trauma is associated with changes in DNA binding activity of nuclear factor of activated T cells (NFAT) and activator protein-1 (AP-1). METHODS: Mice with closed impact injury with fracture in both hind limbs were adopted as the trauma model. Spleen lymphocytes were isolated from traumatized mice and stimulated with Con-A. Culture supernatants were assayed for IL-2 activity, and total RNA was extracted from spleen lymphocytes and assayed for IL-2 mRNA. DNA binding activity of NFAT and AP-1 were measured by electrophoretic mobility shift assay (EMSA). The expression of c-Fos, c-Jun and JunB proteins was determined by the Western blot analysis. RESULTS: DNA binding activity of NFAT and AP-1 gradually decreased to a minimum of 41% and 49%, respectively, of the control on the 4th day after injury, which was closely followed by the decline in IL-2 activity and IL-2 mRNA. A decrease in the expression of c-Fos on the 1st and 4th day after trauma had no significant effect on c-Jun expression; the increase in expression of JunB was only on the 1st day after injury. CONCLUSION: Decreased IL-2 expression is, at least in part, due to a decline in the activation of NFAT and AP-1 in traumatized mice. The decline in DNA binding activity of NFAT and AP-1 is partly due to a trauma-induced block in the expression of c-Fos. 展开更多
关键词 Nuclear proteins Animals Cell Nucleus DNA DNA-Binding proteins Electrophoretic Mobility Shift Assay Female INTERLEUKIN-2 Male Mice NFATC Transcription Factors proto-oncogene proteins c-fos proto-oncogene proteins c-jun RNA Messenger Research Support Non-U.S. Gov't Transcription Factor AP-1 Transcription Factors
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Detection of pim-1 mRNA in prostate cancer diagnosis 被引量:3
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作者 HE Hui-chan BI Xue-cheng +6 位作者 DAI Qi-shan WANG Shao-sheng WEI Hong-ai ZHONG Wei-de LIU Wen-hua JIANG Fu-neng LIU Liang-shi 《Chinese Medical Journal》 SCIE CAS CSCD 2007年第17期1491-1493,共3页
Background Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign pr... Background Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa. Methods The prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve. Results The ratio of pim-1 mRNA to β-actin in the normal prostate, BPH, and PCa were 1.05±0.04, 2.57±0.74 and 4.45±0.63, respectively. The differences among PCa, BPH and NT were significant (P〈0.05, respectively). Conclusion Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa. 展开更多
关键词 proto-oncogene proteins c-pim-1 prostate neoplasms DIAGNOSIS
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Noni(Morinda citrifolia L.) fruit juice delays immunosenescence in the lymphocytes in lymph nodes of old F344 rats 被引量:5
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作者 Uday P.Pratap Hannah P.Priyanka +3 位作者 Karthik R.Ramanathan Vishak Raman Lalgi Hima Srinivasan Thyagarajan 《Journal of Integrative Medicine》 CAS CSCD 2018年第3期199-207,共9页
Objective: Aging is associated with the development of diseases because of immunosuppression and altered functioning of the neuroendocrine system. The medicinal properties of Morinda citrifolia L. have been widely ex... Objective: Aging is associated with the development of diseases because of immunosuppression and altered functioning of the neuroendocrine system. The medicinal properties of Morinda citrifolia L. have been widely exploited for the treatment of age-associated diseases. This study aims to investigate the in vitro and in vivo effects of noni(M. citrifolia) fruit juice(NFJ) on neuro-immunomodulation in the lymph node lymphocytes of F344 rats.Methods: Lymphocytes isolated from axillary and inguinal lymph nodes of young(3–4 months) and old(18–21 months) rats were treated in vitro with different concentrations(0.0001%, 0.01%, and 1%) of NFJ for a period of 24 h. In the in vivo study, old(16–17 months) male F344 rats were treated with 5 mL/kg body weight of 5%, 10% and 20% of NFJ, twice a day, by oral gavage, and lymph node lymphocytes were isolated after 60 d. Concanavalin A(Con A)-induced lymphocyte proliferation, interleukin-2(IL-2)and interferon-γ(IFN-γ) production and expression of intracellular markers, such as phosphoextracellular signal-regulated kinase(p-ERK1/2), phospho-γ AMP response element-binding protein,phospho-protein kinase B(p-Akt), phospho-tyrosine hydroxylase(p-TH), phospho-nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor-α(p-IκB-α) and phospho-nuclear factor-κB(p-NF-κB p65 and p50) were examined in the lymphocytes of lymph nodes.Results: NFJ increased Con A-induced lymphocyte proliferation, IL-2 and IFN-γ production, and p-ERK1/2 expression both in vitro and in vivo. In in vivo NFJ-treated old rats, lymph node lymphocytes showed increased expression of p-TH and Akt, nitric oxide production and decreased expression of p-NF-κB p65 and p50.Conclusion: These results suggest that the immunostimulatory properties of NFJ are facilitated through intracellular signaling pathways involving ERK1/2, Akt and NF-κB. 展开更多
关键词 Tyrosine hydroxylase CYTOKINES Inflammation Mitogen-activated protein kinase 1/2 proto-oncogene proteins Nuclear factor-κB Morinda citrifolia IMMUNOSENESCENCE
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