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两种PGPR菌株诱导马尾松幼苗系统抗病性研究 被引量:1
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作者 吴跃开 朱秀娥 李晓虹 《江苏林业科技》 2008年第3期1-5,共5页
通过科学的试验设计,探讨2种根圈促生细菌Pseudomonas pseudoalcaligens Stanier (P751)和Bacillus cereus Frankland(Bc752)诱导马尾松幼苗系统抗病性的作用,包括不同接种剂量及接种时间的试验效果。试验时,将PG-PR通过灌注方法接种于... 通过科学的试验设计,探讨2种根圈促生细菌Pseudomonas pseudoalcaligens Stanier (P751)和Bacillus cereus Frankland(Bc752)诱导马尾松幼苗系统抗病性的作用,包括不同接种剂量及接种时间的试验效果。试验时,将PG-PR通过灌注方法接种于松苗根部,而将赤枯病病原菌孢子悬浮液喷施于叶面。处理后的松苗置于温室内培养,2个月后测定松苗的感病指数,并检测PGPR在植物体上的定殖。试验结果表明,Bc752能显著地诱导马尾松幼苗的系统抗病性,并以病菌侵染前10 d接种效果最好;施用浓度高时效果较好但不呈直线正相关。P751诱导抗性不太明显,可能是因为长时期保存导致菌种退化,或是P751需要更长的刺激诱导时间。 展开更多
关键词 根围促生细菌 PSEUDOMONAS pseudoalcaligens Stanier (P751) BACILLUS CEREUS Frankland (Bc752) 诱导系统抗病性 马尾松幼苗 松赤枯病
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Isolation and Characterization of Microorganisms for Degradation of Minimal Oils and Fats at Low Temperatures 被引量:1
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作者 陈熹兮 杨虹 +1 位作者 李道棠 李堃宝 《Journal of Shanghai Jiaotong university(Science)》 EI 2003年第2期123-127,共5页
The isolation of microorganisms for biodegradation of minimal fats and oils at low temperatures was reported. By using rapeseed oil as a sole carbon source, six strains were isolated from five kinds of oils/fats-conta... The isolation of microorganisms for biodegradation of minimal fats and oils at low temperatures was reported. By using rapeseed oil as a sole carbon source, six strains were isolated from five kinds of oils/fats-contaminated wastewater, soil, and active sludge. Among them, two strains which show the highest oil removal ratios were identified as Pseudomonas pseudoalcaligenes and Pseudomonas mendocina, respectively. The experiments of orthogonal impact conditions show that the optimal oil degradation condition is at pH 8.0, 5 ℃ and 100 mg/L oil. Under this condition, the rapeseed oil degradation ratios of two strains after 24 hours amount to 92.6% and 92.0% respectively, whereas the removal ratios of lard decrease to 39.5% and 54.3%. 展开更多
关键词 rapeseed oil LARD low temperature bioremediation Pseudomonas pseudoalcaligenes Pseudomonas mendocina
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Studies on Locusts' Pathogenesis and Energy Metabolic Inhibition Induced by the Insecticidal Protein Purified from Pseudomonas Pseudoalcaligenes 被引量:1
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作者 赵建 Zhu +8 位作者 Jianqing Luo Xia Liu Zhu Chen Donghui Yang Zhirong 《High Technology Letters》 EI CAS 2002年第4期13-17,共5页
The insecticidal protein produced by Pseudomonas pseudoalcaligenes is purified from the suspension of the bacterial culture. As an intact molecule, the protein acts on the foregut, midgut, hindgut, vasa Malpighii and ... The insecticidal protein produced by Pseudomonas pseudoalcaligenes is purified from the suspension of the bacterial culture. As an intact molecule, the protein acts on the foregut, midgut, hindgut, vasa Malpighii and fat body, and kills locusts. The disinsection rates of feeding and injection are 63.3% and 65.7%, respectively. After 24h to 48h, it is observed that all these tissues and cells show pathological changes in varying degrees, and so did the host cellular organs of these cells, such as cytoblast, mitochondria, endoplasmic reticulum and ribosome. Particularly, the changes of the mitochondria are much more serious than those of others. Detection of oxygen electrode shows the efficiency of oxidative phosphorylation and the ATP synthesis decreases, while the activity of mitochondrial ATPase is almost not affected. That means the energy utilization of locusts is in gear, but the shortage of the supplying of energy results in their death. This research forms the substantial basis for controlling locusts. 展开更多
关键词 PSEUDOMONAS pseudoalcaligenes INSECTICIDAL protein pathogenesis mitochondria oxidative PHOSPHORYLATION
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Cloning and Identification of promoter of Pseudomonas Pseudoaligenes
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作者 张杰 Zhao +6 位作者 Jian Sun Qun Yin Hongxiang Yang Zhirong 《High Technology Letters》 EI CAS 2003年第3期49-53,共5页
Promoter-probe vector pSUPV4 is used to clone the promoter of Pseudomonas pseudoalcaligenes directly in E. coli, and the recombinant pPA7, which has the highest kanamycin resistance, is obtained. Sequencing the PA7 fr... Promoter-probe vector pSUPV4 is used to clone the promoter of Pseudomonas pseudoalcaligenes directly in E. coli, and the recombinant pPA7, which has the highest kanamycin resistance, is obtained. Sequencing the PA7 fragment discloses several motifs similar to the conservative domains of prokaryotic promoters, including -10 box, -35 box, parallel SD fragment essential to transcription initiation, and the translation initiation site ATG. Southern blotting of PA7 indicates that the PA7 fragment comes from P. pseudoalcaligenes genome and has probably one copy. The PA7 fragment is subcloned by PCR, and the result shows that the 5’-flanking fragment from 889 to 1120 bp has promoter activity, which can be enhanced by the 0.7Kb fragment at 5’ end. The fragments of pPA7 and pPA7-2 are transferred into pseudomonas pseudoaligenes by electroporation, and the significant higher kanamycin resistance of transformants than that of control indicates that the PA7 fragment has the promoter activity in P. pseudoaligene. 展开更多
关键词 E. coli Pseudomonas pseudoalcaligenes PROMOTER promoter-probing vector
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Pseudomonas pseudoalcaligenes B50铁载体合成相关基因pyrD的克隆与功能分析 被引量:4
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作者 丁延芹 黄伟红 +2 位作者 姚良同 于素芳 杜秉海 《微生物学通报》 CAS CSCD 北大核心 2010年第7期981-985,共5页
从棉花根际分离的一株细菌B50在低铁条件下可以产生铁载体。通过形态学特征、生理生化特征及其16SrDNA序列分析,将其鉴定为Pseudomonas pseudoalcaligenes。采用三亲本杂交的方法将转座子Tn5-1063a(含luxAB)导入B50中,进行转座子插入诱... 从棉花根际分离的一株细菌B50在低铁条件下可以产生铁载体。通过形态学特征、生理生化特征及其16SrDNA序列分析,将其鉴定为Pseudomonas pseudoalcaligenes。采用三亲本杂交的方法将转座子Tn5-1063a(含luxAB)导入B50中,进行转座子插入诱变,获得突变株。用TAIL-PCR方法得到与铁吸收有关的pyrD基因序列。通过互补实验验证pyrD与铁载体的合成有关。Pseudomonas pseudoalcaligenes能够产生铁载体属于首次报道。 展开更多
关键词 PSEUDOMONAS pseudoalcaligenes 铁载体合成 pyrD
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Microcystis aeruginosa/Pseudomonas pseudoalcaligenes interaction effects on off-flavors in algae/bacteria co-culture system under different temperatures 被引量:4
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作者 Xi Yang Ping Xie +6 位作者 Yunzhen Yu Hong Shen Xuwei Deng Zhimei Ma Peili Wang Min Tao Yuan Niu 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2015年第5期38-43,共6页
We conducted an experiment to study the interaction effects of Microcystis aeruginosa and Pseudomonas pseudoalcaligenes on off-flavors in an algae/bacteria co-culture system at three temperatures(24, 28 and 32℃). G... We conducted an experiment to study the interaction effects of Microcystis aeruginosa and Pseudomonas pseudoalcaligenes on off-flavors in an algae/bacteria co-culture system at three temperatures(24, 28 and 32℃). Gas chromatography-mass spectrometry was applied to measure off-flavor compounds dimethyl sulfide(DMS), dimethyl trisulfide(DMTS),2-methylisoborneol, geosmin(GEO) and β-cyclocitral. During the lag phase of co-cultured M. aeruginosa(first 15 days), P. pseudoalcaligenes significantly increased the production of DMS, DMTS and β-cyclocitral at all three temperatures. In the exponential phase of co-cultured M. aeruginosa(after 15 days), M. aeruginosa became the main factor on off-flavors in the co-culture system, and β-cyclocitral turned to the highest off-flavor compound. These results also indicated that DMS, DMTS and β-cyclocitral were the main off-flavor compounds in our M. aeruginosa/P. pseudoalcaligenes co-culture system. Univariate analysis was applied to investigate the effects of M. aeruginosa and P. pseudoalcaligenes on the production of off-flavors. The results demonstrated that both M. aeruginosa and P. pseudoalcaligenes could increase the production of DMS and DMTS, while β-cyclocitral was mainly determined by M. aeruginosa. Our results also provide some insights into understanding the relationship between cyanobacteria and heterotrophic bacteria. 展开更多
关键词 Co-culture system Microcystis aeruginosa Off-flavors Pseudomonas pseudoalcaligenes
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Formation of Polyhydroxyalkanoate Blends by Pseudomonas pseudoalcaligenes M1-2 from Various Carbon Sources
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作者 吴琼 张广 +3 位作者 田卫东 孙素琴 张日清 陈国强 《Tsinghua Science and Technology》 SCIE EI CAS 1999年第3期1539-1543,共5页
Pseudomonas pseudoalcaligenes strain M1\|2 isolated from oil\|contaminated soil collected from an oilfield in northern China was found to be able to synthesize a blend of polyhydroxyalkanoates (PHAs) containing mono... Pseudomonas pseudoalcaligenes strain M1\|2 isolated from oil\|contaminated soil collected from an oilfield in northern China was found to be able to synthesize a blend of polyhydroxyalkanoates (PHAs) containing monomers of 3\|hydroxybutyrate (C4), 3\|hydroxyvalerate (C5), 3\|hydroxyheptanoate (C7), 3\|hydroxyoctanoate (C8), 3\|hydroxynonanoate (C9), 3\|hydroxydecanoate (C10) and 3\|hydroxydodecanoate (C12) from various carbon sources. The hydroxyalkanoate (HA) monomer composition varied both quantitatively and qualitatively, depending on the carbon sources used. The presence of octanoate in substrates of myristic acid or tridecanoate promoted the synthesis of HB monomer in the blend. Concentration of octanoate was also found to significantly affect the PHB content in the blend. A PHA biosynthesis pathway in Pseudomonas pseudoalcaligens M1\|2 was proposed. 展开更多
关键词 POLYHYDROXYALKANOATE PHA POLYHYDROXYBUTYRATE PHB Pseudomonas pseudoalcaligenes
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