Twenty four male guinea-pigs were randomly divided into 3 groups: Group A, allergic asthmaguinea-pigs prepared by peritoneal injection and spraying inhalation of albumin; Group B, allergic asthmaguinea-pigs treated wi...Twenty four male guinea-pigs were randomly divided into 3 groups: Group A, allergic asthmaguinea-pigs prepared by peritoneal injection and spraying inhalation of albumin; Group B, allergic asthmaguinea-pigs treated with electroacupuncture through stimulating the acupoint Feishu (UB13 ) and Group C, thecontrol group. With[3H] -DHA as a radioligand, pulmonary adrenoceptor ( AC) in the 3 groups of guinea-pigs were detrermined by radioligand binding assay technique. The results showed that: ( 1 ) The maximumbinding volume of receptor ligand ( Bmax, fmol/mg prot. ) of pulmonary AC in Group A (123. 86 42. 91 )was significantly lower than that in group C (199 . 54 37. 03 , P< 0. 05) , while the difference between groupB ( 142. 00 42. 91 ) and group C was not significant ( P > 0. 05 ) . ( 2 ) The Kd ( nM) of pulmonary AC ingroup A, B and C were 5 . 10 1 . 39 , 6. 53 2 . 41 and 8. 72 2 . 02 respectively, and the differences amongthe three groups were not significant ( P >0. 05) . The results indicated that the decrease of the content anddysfunction of pulmonary AC in allergic asthma guinea-pigs might be regulated by electroacupuncture thera-py.展开更多
文摘Twenty four male guinea-pigs were randomly divided into 3 groups: Group A, allergic asthmaguinea-pigs prepared by peritoneal injection and spraying inhalation of albumin; Group B, allergic asthmaguinea-pigs treated with electroacupuncture through stimulating the acupoint Feishu (UB13 ) and Group C, thecontrol group. With[3H] -DHA as a radioligand, pulmonary adrenoceptor ( AC) in the 3 groups of guinea-pigs were detrermined by radioligand binding assay technique. The results showed that: ( 1 ) The maximumbinding volume of receptor ligand ( Bmax, fmol/mg prot. ) of pulmonary AC in Group A (123. 86 42. 91 )was significantly lower than that in group C (199 . 54 37. 03 , P< 0. 05) , while the difference between groupB ( 142. 00 42. 91 ) and group C was not significant ( P > 0. 05 ) . ( 2 ) The Kd ( nM) of pulmonary AC ingroup A, B and C were 5 . 10 1 . 39 , 6. 53 2 . 41 and 8. 72 2 . 02 respectively, and the differences amongthe three groups were not significant ( P >0. 05) . The results indicated that the decrease of the content anddysfunction of pulmonary AC in allergic asthma guinea-pigs might be regulated by electroacupuncture thera-py.
