Background: Pulp stones are calcified masses formed in the primary and permanent dentitions. The ischemic cardiovascular disorders (CVD) can be the first health problem of the world. It seems that there is a relations...Background: Pulp stones are calcified masses formed in the primary and permanent dentitions. The ischemic cardiovascular disorders (CVD) can be the first health problem of the world. It seems that there is a relationship between pulp stones and cardiovascular diseases to determine the diagnostic value of panoramic dental radiographs as non-invasive test for the early detection of CVD. Methods and Materials: The subjects of the study came from patients presenting to Dental Radiology Department of Dental School in Yazd who aged 30 - 64 years, had 8 natural teeth. They were referred to the Cardiovascular Center of Afshar Hospital in Yazd for cardiovascular evaluation. To set the cut-off point, the ratio of teeth with pulp stones to the total number of teeth for each person was calculated and also ABI for diagnosing IHD via ROC curve was used. Results: Only 3.8% of patients without pulp stone were affected by ischemic heart disease (IHD). About Sixty seven percent (67.3%) of individuals had at least one tooth with pulp stone. In individuals without IHD, 5% of the teeth showed pulp stone while this rate was 45% (9 times) in CVD patients. The number of patients for whom the ratio of teeth with pulp stone to total number of teeth was 0.2 or more, was 138.7 times greater than the other. Conclusion: The dentists, who order panoramic radiographs for the treatment of patients, exactly study those teeth for the presence of pulp stones. The ratio of teeth with pulp stone to total teeth equal 0.2 or more was a good tool for early detection of CVD.展开更多
Acute liver failure is a refractory disease and its pro-gnosis, if not treated using liver transplantation, is extremely poor. It is a good candidate for regenerative medicine, where stem cell-based therapies play a c...Acute liver failure is a refractory disease and its pro-gnosis, if not treated using liver transplantation, is extremely poor. It is a good candidate for regenerative medicine, where stem cell-based therapies play a central role. Mesenchymal stem cells(MSCs) are known to differentiate into multiple cell lineages including hepatocytes. Autologous cell transplant without any foreign gene induction is feasible using MSCs, thereby avoiding possible risks of tumorigenesis and immune rejection. Dental pulp also contains an MSC population that differentiates into hepatocytes. A point worthy of special mention is that dental pulp can be obtained from deciduous teeth during childhood and can be subsequently harvested when necessary after deposition in a tooth bank. MSCs have not only a regenerative capacity but also act in an anti--inflammatory manner via paracrine mechanisms. Promising efficacies and difficulties with the use of MSC derived from teeth are summarized in this review.展开更多
The hippocampal formation, important for spatial learn- ing and memory function, exhibits high level of plas- ticity in response to behavioral changes as well as injury. Dysfunction of the hippocampus is one of the ha...The hippocampal formation, important for spatial learn- ing and memory function, exhibits high level of plas- ticity in response to behavioral changes as well as injury. Dysfunction of the hippocampus is one of the hallmark features of neurodegenerative diseases like temporal lobe epilepsy （TLE） and Alzheimer＇s disease （AD） （Dhanush- kodi and Shetty 2008）. Excitotoxicity is one of the known mechanisms by which neurons undergo degeneration in neurodegenerative condition （Haglid et al., 1994; Doble et al., 1995）. Brain regions such as hippocampus are more susceptible to excitotoxic damage. During excitotoxicity.展开更多
Dental pulp stem cells are dental pulp-derived mesenchymal stem cells that originate from the neural crest.They exhibit greater potential for the treatment of nervous system diseases than other types of stem cells bec...Dental pulp stem cells are dental pulp-derived mesenchymal stem cells that originate from the neural crest.They exhibit greater potential for the treatment of nervous system diseases than other types of stem cells because of their neurogenic differentiation capability and their ability to secrete multiple neurotrophic factors.Few studies have reported Alzheimer’s disease treatment using dental pulp stem cells.Rat models of Alzheimer’s disease were established by injecting amyloid-β1–42 into the hippocampus.Fourteen days later,5×106 dental pulp stem cells were injected into the hippocampus.Immunohistochemistry and western blot assays showed that dental pulp stem cell transplantation increased the expression of neuron-related doublecortin,NeuN,and neurofilament 200 in the hippocampus,while the expression of amyloid-βwas decreased.Moreover,cognitive and behavioral abilities were improved.These findings indicate that dental pulp stem cell transplantation in rats can improve cognitive function by regulating the secretion of neuron-related proteins,which indicates a potential therapeutic effect for Alzheimer’s disease.This study was approved by the Animal Ethics Committee of Harbin Medical University,China(approval No.KY2017-132)on February 21,2017.展开更多
Neurodegenerative diseases and neural injury are 2 of the most feared disorders that afflict humankind by leading to permanent paralysis and loss of sensation.Cell based treatment for these diseases had gained special...Neurodegenerative diseases and neural injury are 2 of the most feared disorders that afflict humankind by leading to permanent paralysis and loss of sensation.Cell based treatment for these diseases had gained special interest in recent years.Previous studies showed that dental pulp stem cells(DPSCs) could differentiate toward functionally active neurons both in vitro and in vivo,and could promote neuranagenesis through both cell-autonomous and paracrine neuroregenerative activities.Some of these neuroregenerative activities were unique to tooth-derived stem cells and superior to bone marrow stromal cells.However,DPSCs used in most of these studies were mixed and unfractionated dental pulp cells that contain several types of cells,and most were fibroblast cells while just contain a small portion of DPSCs.Thus,there might be weaker ability of neuranagenesis and more side effects from the fibroblast cells that cannot differentiate into neural cells.p75 neurotrophin receptor(p75 NTR) positive DPSCs subpopulation was derived from migrating cranial neural crest cells and had been isolated from DPSCs,which had capacity of differentiation into neurons and repairing neural system.In this article,we hypothesize that p75 NTR positive DPSCs simultaneously have greater propensity for neuronal differentiation and fewer side effects from fibroblast,and in vivo transptantation of autologous p75 NTR positive DPSCs is a novel method for neuranagenesis.This will bring great hope to patients with neurodegenerative disease and neural injury.Supported by Key Basic Research Fund of Science and Technology Commission of Shanghai Municipality(10JC1408700).展开更多
文摘Background: Pulp stones are calcified masses formed in the primary and permanent dentitions. The ischemic cardiovascular disorders (CVD) can be the first health problem of the world. It seems that there is a relationship between pulp stones and cardiovascular diseases to determine the diagnostic value of panoramic dental radiographs as non-invasive test for the early detection of CVD. Methods and Materials: The subjects of the study came from patients presenting to Dental Radiology Department of Dental School in Yazd who aged 30 - 64 years, had 8 natural teeth. They were referred to the Cardiovascular Center of Afshar Hospital in Yazd for cardiovascular evaluation. To set the cut-off point, the ratio of teeth with pulp stones to the total number of teeth for each person was calculated and also ABI for diagnosing IHD via ROC curve was used. Results: Only 3.8% of patients without pulp stone were affected by ischemic heart disease (IHD). About Sixty seven percent (67.3%) of individuals had at least one tooth with pulp stone. In individuals without IHD, 5% of the teeth showed pulp stone while this rate was 45% (9 times) in CVD patients. The number of patients for whom the ratio of teeth with pulp stone to total number of teeth was 0.2 or more, was 138.7 times greater than the other. Conclusion: The dentists, who order panoramic radiographs for the treatment of patients, exactly study those teeth for the presence of pulp stones. The ratio of teeth with pulp stone to total teeth equal 0.2 or more was a good tool for early detection of CVD.
基金Supported by A Grant-in-Aid for Scientific Research(C)(25461012 to Shogo Ohkoshi)from the Japan Society for the Promotion of Science(JSPS)
文摘Acute liver failure is a refractory disease and its pro-gnosis, if not treated using liver transplantation, is extremely poor. It is a good candidate for regenerative medicine, where stem cell-based therapies play a central role. Mesenchymal stem cells(MSCs) are known to differentiate into multiple cell lineages including hepatocytes. Autologous cell transplant without any foreign gene induction is feasible using MSCs, thereby avoiding possible risks of tumorigenesis and immune rejection. Dental pulp also contains an MSC population that differentiates into hepatocytes. A point worthy of special mention is that dental pulp can be obtained from deciduous teeth during childhood and can be subsequently harvested when necessary after deposition in a tooth bank. MSCs have not only a regenerative capacity but also act in an anti--inflammatory manner via paracrine mechanisms. Promising efficacies and difficulties with the use of MSC derived from teeth are summarized in this review.
基金supported by Science and Engineering Research Board,Government of India to AD(SB/YS/LS-122/2013)
文摘The hippocampal formation, important for spatial learn- ing and memory function, exhibits high level of plas- ticity in response to behavioral changes as well as injury. Dysfunction of the hippocampus is one of the hallmark features of neurodegenerative diseases like temporal lobe epilepsy （TLE） and Alzheimer＇s disease （AD） （Dhanush- kodi and Shetty 2008）. Excitotoxicity is one of the known mechanisms by which neurons undergo degeneration in neurodegenerative condition （Haglid et al., 1994; Doble et al., 1995）. Brain regions such as hippocampus are more susceptible to excitotoxic damage. During excitotoxicity.
基金This study was supported by Yu Weihan Fund for Distinguished Young Scholars of Harbin Medical University of China,No.002000013(to XMZ).
文摘Dental pulp stem cells are dental pulp-derived mesenchymal stem cells that originate from the neural crest.They exhibit greater potential for the treatment of nervous system diseases than other types of stem cells because of their neurogenic differentiation capability and their ability to secrete multiple neurotrophic factors.Few studies have reported Alzheimer’s disease treatment using dental pulp stem cells.Rat models of Alzheimer’s disease were established by injecting amyloid-β1–42 into the hippocampus.Fourteen days later,5×106 dental pulp stem cells were injected into the hippocampus.Immunohistochemistry and western blot assays showed that dental pulp stem cell transplantation increased the expression of neuron-related doublecortin,NeuN,and neurofilament 200 in the hippocampus,while the expression of amyloid-βwas decreased.Moreover,cognitive and behavioral abilities were improved.These findings indicate that dental pulp stem cell transplantation in rats can improve cognitive function by regulating the secretion of neuron-related proteins,which indicates a potential therapeutic effect for Alzheimer’s disease.This study was approved by the Animal Ethics Committee of Harbin Medical University,China(approval No.KY2017-132)on February 21,2017.
文摘Neurodegenerative diseases and neural injury are 2 of the most feared disorders that afflict humankind by leading to permanent paralysis and loss of sensation.Cell based treatment for these diseases had gained special interest in recent years.Previous studies showed that dental pulp stem cells(DPSCs) could differentiate toward functionally active neurons both in vitro and in vivo,and could promote neuranagenesis through both cell-autonomous and paracrine neuroregenerative activities.Some of these neuroregenerative activities were unique to tooth-derived stem cells and superior to bone marrow stromal cells.However,DPSCs used in most of these studies were mixed and unfractionated dental pulp cells that contain several types of cells,and most were fibroblast cells while just contain a small portion of DPSCs.Thus,there might be weaker ability of neuranagenesis and more side effects from the fibroblast cells that cannot differentiate into neural cells.p75 neurotrophin receptor(p75 NTR) positive DPSCs subpopulation was derived from migrating cranial neural crest cells and had been isolated from DPSCs,which had capacity of differentiation into neurons and repairing neural system.In this article,we hypothesize that p75 NTR positive DPSCs simultaneously have greater propensity for neuronal differentiation and fewer side effects from fibroblast,and in vivo transptantation of autologous p75 NTR positive DPSCs is a novel method for neuranagenesis.This will bring great hope to patients with neurodegenerative disease and neural injury.Supported by Key Basic Research Fund of Science and Technology Commission of Shanghai Municipality(10JC1408700).
