Model prediction of inactivation of Aeromonas salmonicida grown on poultry in situ by intense pulsed light

The aim of this study was to evaluate the factors influencing the inactivation effect of intense pulsed light(IPL)on Aeromonas salmonicida grown on chicken meat and skin,and to further develop prediction models of inactivation.In this work,chicken meat and skin inoculated with meat-borne A.salmonicida isolates were subjected to IPL treatments under different conditions.The results showed that IPL had obvious bactericidal effect in the chicken skin and thickness groups when the treatment voltage and time were 7 V combined with 5 s.In addition,the lethality curves of A.salmonicida were fitted under IPL conditions of 3.5-7.5 V.The comparison of statistical parameters revealed that the Weibull model could best fit the mortality curves and could accurately predict the mortality dynamic of A.salmonicida grown on chicken skin.And further a secondary model between the scale factor b and the treatment voltage in Weibull model was established using linear equations,which determined that the secondary model could accurately predict the inactivation of A.salmonicida.This study provides a theoretical basis for future prediction models of Aeromonas,and also provides new ideas for sterilization approaches of meat-borne Aeromonas.

Non-thermal ablation of rabbit liver VX2 tumor by pulsed high intensity focused ultrasound with ultrasound contrast agent:Pathological characteristics

AIM： To investigate the pathological characteristics of non-thermal damage induced by pulsed high intensity focused ultrasound （PHIFU） combined with ultrasound contrast agent （UCA）, SonoVue （Bracco SpA, Milan, Italy） in rabbit liver VX2 tumor. METHODS： Liver VX2 tumor models were established in 20 rabbits, which were divided randomly into PHIFU combined with ultrasound contrast agent group （PHIFU ＋ UCA group） and sham group. In the PHIFU ＋ UCA group, 0.2 mL of SonoVue was injected intravenously into the tumor, followed by ultrasound exposure of Isp 5900 W/cm^2. The rabbits were sacrificed one day after ultrasound exposure. Specimens of the exposed tumor tissues were obtained and observed pathologically under light microscope and transmission electron microscope. The remaining tumor tissues were sent for 2,3,5-Triphenyltetrazolium chloride （TTC） staining. RESULTS： Before Trc staining, tumor tissues in both the sham and the PHIFU ＋ UCA groups resembled gray fish meat, After TIC staining, the tumor tissues were uniformly stained red, with a clear boundary between tumor tissue and normal tissue, Histological examination showed signs of tumor cell injury in PHIFU ＋ UCA group, with cytoplasmic vacuoles of various sizes, chromatin margination and karyopyknosis. Electron microscopic examination revealed tumor cell volume reduction, karyopyknosis, chromatin margination, intercellular space widening, the presence of high electro＇n-density apoptotic bodies and vacuoles in cytoplasm. CONCLUSION： The non-thermal effects of PHIFU combined with UCA can be used to ablate rabbit liver VX2 tumors.

Osteogenic Effect of Low Intensity Pulsed Ultrasound on Rat Adipose-derived Stem Cells in vitro

The osteogenic in vitro effect of low intensity pulsed ultrasound (LIPUS) on SD rat adi-pose-derived stem cells (ADSCs) was investigated.Rat ADSCs underwent LIPUS (intensity=100 mW/cm2) or sham exposure for 8 min per treatment once everyday in vitro,and then the alkaline phos-phatase (ALP) activity and mineralized nodule formation were assessed to evaluate the osteogenic effect of LIPUS on ADSCs.To further explore the underlying mechanism,the osteogenic-related gene mRNA expression was determined by using reverse transcriptase-polymerase chain reaction (RT-PCR) at 1st,3rd,5th,7th day after exposure repectively.Westen blot was used to evaluate the protein expression levels of two osteogenic differentiation associated genes at 7th and 14th day repectively.It was found that ALP activity was increased after LIPUS exposure and LIPUS resulted in mineralized nodule formation of ADSCs in vitro.LIPUS-treated ADSCs displayed higher mRNA expression levels of runt-related transcription factor 2 (Runx2),osteocalcin (OCN),ALP and bone sialoprotein (BSP) genes than con-trols,and the protein levels of Runx2 and BSP were also increased.The results suggested that LIPUS may induce the osteogenic differentiation of ADSCs in vitro.

The Effect of Intense Pulsed Light Treatment for Chronic Hordeolum

Objective To evaluate the effect of intense pulsed light(IPL)in the treatment of chronic hordeolum.Methods Patients with chronic hordeolum who underwent IPL treatment were enrolled in this study.According to the severity of hordeolum,the patients were treated with IPL 3 to 5 times.Patients’satisfaction and visual analog scale scores for ocular discomfort symptoms before and after treatment were collected.The number,congestion,long diameter,short diameter and area of nodules were also recorded and measured.Finally,eyelid margin signs,meibum quality,meibomian gland expressibility,meibomian gland dropout,tear meniscus height,and corneal fluorescein staining were scored.Results 20 patients were enrolled in this study.The eyelid margins were congestive and swollen,with blunt rounding or irregularity.The meibum was cloudy or toothpaste-like.The meibomian gland expressibility,meibomian gland dropout and tear meniscus height were reduced.The cornea showed scattered fluorescein staining.After treatment,score of visual analog scale,congestion and size of nodules were significantly reduced.Eyelid margin signs,meibum quality,meibomian gland expressibility,tear meniscus height and corneal fluorescein staining scores were improved.Meibomian gland dropout had no significant change.No side effects occurred during treatment.Conclusions IPL is beneficial for the treatment of chronic hordeolum.

Study on equivalent velocity pulse of nearfault ground motions

Near-fault strong ground motions that resulted in serious structural damage are characterized by directivity effect and pulse-type motion. Large-amplitude and long-period pulses are contained in the velocity time-history traces of near-fault pulse-type records. A reasonable model of equivalent velocity pulse is proposed on the basis of the ex- isted models in this paper to simplify the calculation and analysis. Based on the large amount of collected near-fault strong earthquakes records, the parameters describing equivalent velocity pulse model such as pulse period, pulse intensity and number of predominant pulses are studied, and comparison is made with the results obtained by others models. The proposed model is contributive to the seismic design for structures in near-fault areas.

Fifty Cases of Chloasma Treated by Acupuncture plus Intensive Pulse Light Irradiation

Objective: To observe the clinical therapeutic effect of acupuncture plus intensive pulse light irradiation on chloasma. Methods: Ninety-six cases of chloasma were randomly divided into two groups, the control group of 46 cases treated by simple acupuncture and the treatment group of 50 cases treated by acupuncture and intensive pulse light irradiation. Results: The total effective rate was 89.1% and 98.0% in the control group and treatment group respectively, with a significant difference between the two groups (P<0.05). Conclusion: For chloasma, the effect of treatment with acupuncture plus intensive pulse light irradiation is superior to that with simple acupuncture.

Novel intense pulsed light-photodynamic therapy strategy in the treatment of facial actinic keratoses concomitant with rosacea

Rosacea presents as transient or persistent erythema,papules,pustules,fAushing,and telangi-ectasia on the middle of the face,which has some clinical similarity with act inic keratosis(AK).These two conditions can coexist in the same patient.Dermoscopy and reflect ance confocal microscopy(RCM)could be useful methods for diagnosis and monitoring treatment efficacy.Novel intense pulsed light-photodynamic therapy(IPL-PDT)may have better tolerance and curative effect than traditional red-light 5-aminolevulinic acid photodynamic therapy(ALA-PDT).Herein,we present a case of facial AKs concomitant with rosacea where combination therapy of novel IPL-PDT and oral minocycline was effective in that AK lesions were eliminated and the patient's facial erythema and telangiectasia were significantly improved.

Study on the interaction of intense femtosecond laser pulses with nanometre-sized hydrogen clusters

The interaction of intense femtosecond laser pulses with hydrogen clusters has been experimentally studied. The hydrogen clusters were produced from expansion of high-pressure hydrogen gas （backed up to 8×10^6Pa） into vacuum through a conical nozzle cryogenically cooled by liquid nitrogen. The average size of hydrogen clusters was estimated by Rayleigh scattering measurement and the maximum proton energy of up to 4.2keV has been obtained from the Coulomb explosion of hydrogen clusters under 2 × 10^16W/cm^2 laser irradiation. Dependence of the maximum proton energy on cluster size and laser intensity was investigated, indicating the correlation between the laser intensity and the cluster size. The maximum proton energy is found to be directly proportional to the laser intensity, which is consistent with the theoretical prediction.

Response characteristics of sonar receiver under intense sound pulse

For anti-jamming and anti-countermeasure techniques of the sonar receiver, the response characteristics of the automatic gain control （AGC） circuit and the survivability of the prime circuit under strong interference are analyzed by simulations and experiments. An AGC simulation model based on the voltage control amplifier VCA810 prototype is proposed. Then static and dynamic simulations are realized with single frequency signal and linear frequency modulated （LFM） signal commonly used in the active sonar. Based on intense sound pulse （ISP） interference experiments, the real-time response characteristics of each module of the receiver are studied to verify the correctness of the model as well as the simulation results. Simulation and experiment results show that, under 252 dB/20 μs ISP interference, the specific sonar receiver will produce sustained cut top oscillation above 30 ms, which may affect the receiver and block the regular sonar signal.

Core-Excited Molecules by Resonant Intense X-Ray Pulses Involving Electron-Rotation Coupling

It has been reported that electron-rotation coupling plays a significant role in diatomic nuclear dynamics induced by intense VUV pulses [Phys. Rev. A 102(2020) 033114;Phys. Rev. Res. 2(2020) 043348]. As a further step, we present here investigations of the electron-rotation coupling effect in the presence of Auger decay channel for core-excited molecules, based on theoretical modeling of the total electron yield(TEY), resonant Auger scattering(RAS) and x-ray absorption spectra(XAS) for two showcases of CO and CH^(+) molecules excited by resonant intense x-ray pulses. The Wigner D-functions and the universal transition dipole operators are introduced to include the electron-rotation coupling for the core-excitation process. It is shown that with the pulse intensity up to 10^(16) W/cm^(2), no sufficient influence of the electron-rotation coupling on the TEY and RAS spectra can be observed. This can be explained by a suppression of the induced electron-rotation dynamics due to the fast Auger decay channel, which does not allow for effective Rabi cycling even at extreme field intensities,contrary to transitions in optical or VUV range. For the case of XAS, however, relative errors of about 10% and 30% are observed for the case of CO and CH^(+), respectively, when the electron-rotation coupling is neglected.It is concluded that conventional treatment of the photoexcitation, neglecting the electron-rotation coupling,can be safely and efficiently employed to study dynamics at the x-ray transitions by means of electron emission spectroscopy, yet the approximation breaks down for nonlinear processes as stimulated emission, especially for systems with light atoms.

Quantum Path Selection and Isolated-Attosecond-Pulse Generation of H2+ with an Intense Laser Pulse and a Static Field

We theoretically investigate the high-order-harmonic generation from the H2^＋ molecular ion exposed to the combi- nation of an intense trapezoidal laser and a static field. The results show that the harmonic spectrum is obviously extended and the short quantum path is selected to contribute to the spectrum, because the corresponding long path is seriously suppressed. Then the combined Coulomb and laser field potentials and the time-dependent electron wave packet distributions are applied to illustrate the physical mechanism of high-order harmonic gen- eration. Finally, by adjusting the intensity of the static field and superposing a properly selected range of the HHG spectrum, a 90-as isolated attosecond pulse is straightforwardly obtained.

Stimulated Emission of Gamma Photon from Ultrashort Pulse Intense Laser—Solid—Target Interaction

The efficient production of energetic γ photons is a significant physical process in the relativistic ultrashortpulse laser-plasma inducing photonuclear action. Based on the interaction of laser-solid-target, an analytical theory onstimulated γ photon emission from a hot electron firing the target-nucleus is developed by a relativistic full quantummethod. The emitting power or probability of γ photon in arbitrary space direction can be calculated for laser irradiatingsolid-target normally. It is valid only if the scatter-centre is immovable or its motion can be neglected compared withthat of the scattered electrons.

Coulomb expansion of deuterated methane clusters irradiated by an ultrashort intense laser pulse

The simulations of three-dimensional particle dynamics show that when irradiated by an ultrashort intense laser pulse, the deuterated methane cluster expands and the majority of deuterons overrun the more slowly expanding carbon ions, resulting in the creation of two separated subelusters. The enhanced deuteron kinetic energy and a narrow peak around the energy maximum in the deuteron energy distribution make a considerable contribution to the efficiency of nuclear fusion compared with the ease of homonuelear deuterium clusters. With the intense laser irradiation, the nuclear fusion yield increases with the increase of the cluster size, so that deuterated heteronuelear clusters with larger sizes are required to achieve a greater neutron yield.

Modulation of ionization on laser frequency in ultra-short pulse intense laser-gas-target

Based on the dispersion relation of intense laser pulse propagating in gradually ionized plasma, this paper discusses the frequency modulation induced by ionization of an ultra-short intense laser pulse interacting with a gas target. The relationship between the frequency modulation and the ionization rate, the plasmas frequency variation, and the polarization of atoms （ions） is analysed. The numerical results indicate that, at high frequency, the polarization of atoms （ions） plays a more important role than plasma frequency variation in modulating the laser frequency, and the laser frequency variation is different at different positions of the laser pulse.

Gravitational Perturbation of Hydrogen Spectrum in the Space Curved by Intense Laser Pulses

The energy level shifts of hydrogen in the space curved by the intense short laser pulses are studied. It shows that for present power level of laser pulses, the magnitude of the energy level shifts in a highly excited hydrogen atom is detectable.

Dynamics simulation on the interaction of intense laser pulses with atomic clusters

Under classical particle dynamics, the interaction process between intense femtosecond laser pulses and icosahedral noble-gas atomic clusters was studied. Our calculated results show that ionization proceeds mainly through tunnel ionization in the combined field from ions, electrons and laser, rather than the electron-impact ionization. With increasing cluster size, the average and maximum kinetic energy of the product ion increases. According to our calculation, the expansion process of the clusters after laser irradiation is dominated by Coulomb explosion and the expansion scale increases with increasing cluster size. The dependence of average kinetic energy and average charge state of the product ions on laser wavelength is also presented and discussed. The dependence of average kinetic energy on the number of atoms inside the cluster was studied and compared with the experimental data. Our results agree with the experimental results reasonably well.

Nonlinear propagation of an intense Laguerre-Gaussian laser pulse in a plasma channel

The nonlinear propagation of an intense Laguerre-Gaussian(LG)laser pulse in a parabolic preformed plasma channel is analyzed by means of the variational method.The evolution equation of the spot size is derived including the effects of relativistic self-focusing,preformed channel focusing,and ponderomotive self-channeling.The parametric conditions of the LG laser pulse and plasma channel for propagating with constant spot size,periodically focusing and defocusing oscillation,catastrophic focusing,and solitary waves are obtained.Compared with the laser pulse with fundamental Gaussian(FG)mode,it is found that the effect of vacuum diffraction is reduced by half and the effects of relativistic and wakefield focusing are decreased by a quarter due to the hollow transverse intensity profile of the LG laser pulse,while the effect of channel focusing is the same order of magnitude with that of the FG laser pulse.Thus,the matched condition for the intense LG laser pulse with constant spot size is released obviously,while the parameters of the laser and plasma for the existence of solitary waves nearly coincide with those of the FG laser pulse.

Propagation of Intense Femtosecond Laser Pulses in Deuterated Methane and Deuterium Cluster Jets

Propagation of intense femtosecond laser pulses （60 fs, 800 nm, 120 m J, 6 × 10^17 W/cm^2 in vacuum） in supersonic （CD4）N and （D2）N cluster jets at different backing pressures was studied. Pump-probe interferometry is employed to investigate the propagation of laser beams in dense cluster jets by examining the electron density distribution of plasma chan- nels. It was found that propagation effects, including ionization-induced defocusing and laser attenuation of incident pulses, are very different in the （CD4）N and （D2）N cluster jets. Different ionization states of CD4 and D2 molecules were observed by analyzing the transverse electron density profiles of the plasma channels and should be considered as a major reason for the differences in the propagation effects. Numerical simulations of the nonlinear propagation of femtosecond laser pulses in （CD4）N and （D2）N cluster jets were performed, and the results indicated a good reproduction of the experimental data.

Ultrafast photoionization of ions and molecules by orthogonally polarized intense laser pulses: Effects of the time delay

We present the photoelectron momentum distributions(PMDs) and the photoelectron angular distributions(PADs) of He+ ions, aligned H2+ molecules and N2 molecules by intense orthogonally polarized laser pulses. Simulations are performed by numerically solving the corresponding two-dimensional time-dependent Schr?dinger equations(TDSEs) within the single-electron approximation frame. Photoelectron momentum distributions and photoelectron angular distributions present different patterns with the time delays Td, illustrating the dependences of the PMDs and PADs on the time delays by orthogonally polarized laser pulses. The evolution of the electron wavepackets can be employed to describe the intensity of the PADs from the TDSE simulations for N2 molecules.