BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs ...BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.展开更多
Modular multilevel converters(MMCs) operate in the low modulation index region in many applications.However, when utilized at the low modulation index region, large harmonics appear in the output voltage, which degrad...Modular multilevel converters(MMCs) operate in the low modulation index region in many applications.However, when utilized at the low modulation index region, large harmonics appear in the output voltage, which degrade the performance of the MMC. To improve the harmonic characteristic in the low modulation index, the carrier dynamic overlapping switching frequency optimal pulse width modulation(CDOSFOPWM) method is proposed for the MMC here. The whole modulation index region is divided into three regions: high modulation index region, middle modulation index region, and low modulation index region. The carrier amplitude, carrier overlap ratio, and frequency of triangular carriers are chosen dynamically according to the modulation index region of the modulation signals, to achieve the optimal harmonic characteristic in the whole modulation index region and maintain the switching loss. The number of on-state submodules(SMs) can be calculated by CDOSFOPWM, and the selection of SMs is performed by a reducing switching frequency voltage balancing algorithm. Finally, the proposed method is verified by simulation and experimental results.展开更多
该文以三相电压型脉宽调制(pulse width modulation,PWM)整流器为研究对象,提出一种基于新型开关表的直接功率控制(direct power control,DPC)策略。首先,根据两相旋转dq坐标系下整流器数学模型计算开关矢量对有功功率和无功功率的控制...该文以三相电压型脉宽调制(pulse width modulation,PWM)整流器为研究对象,提出一种基于新型开关表的直接功率控制(direct power control,DPC)策略。首先,根据两相旋转dq坐标系下整流器数学模型计算开关矢量对有功功率和无功功率的控制幅度,并归一化处理进行定量分析,以此为基础将传统DPC的12扇区重新划分为24扇区。然后,设计有功功率差值区间判断器,根据其输出值Cp和有功功率与无功功率滞环比较器的输出值Sp、Sq,选择当前扇区最佳开关矢量构造新型开关表,实现对有功功率的精确控制。仿真对比表明,该新型开关表不仅克服传统开关表对无功功率控制能力差的缺点,而且改善网侧电流总谐波失真,降低输出直流电压波动和有功功率与无功功率脉动。最后,搭建一台800W三相电压型PWM整流器样机,通过对比实验验证所提控制策略的正确性和有效性。展开更多
Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for...Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems.展开更多
Matrix converter fed motor drive is superior to pulse width modulation inverter drives since it not only provides bi-directional power flow,sinusoidal input/output currents,unity input power factor,but also allows a c...Matrix converter fed motor drive is superior to pulse width modulation inverter drives since it not only provides bi-directional power flow,sinusoidal input/output currents,unity input power factor,but also allows a compact design due to the lack of DC-link capacitors for energy storage.In this paper,model and control of matrix converter fed induction motor drive system are analyzed.A combined control strategy is simplified and improved,which realizes space vector pulse width modulation of matrix converter and rotor flux oriented vector control technique for induction motor drive simultaneously.This control strategy combines the advantages of matrix converter with the good drive performance of vector control technique.Experimental results demonstrate the feasibility and effectiveness of the proposed control strategy.展开更多
This paper proposes the modeling and simulation technique to analyze and design a Boost converter using generalized minimum variance method with discrete-time quasi-sliding mode to adjust the converter switch through ...This paper proposes the modeling and simulation technique to analyze and design a Boost converter using generalized minimum variance method with discrete-time quasi-sliding mode to adjust the converter switch through a pulse width modulation (PWM), so as to enhance a stable output voltage. The control objective is to maintain the sensed output voltage stable, constant and equal to some constant reference voltage (8 volt) in the load resistance variation (24, 48, 240) Ω and input voltage variation (20, 24, 28) volt circumstances. This control strategy is very appropriate for the digitally controlled power converter and for the system requirement accomplishment, resulting high output voltage accuracy. The performance degradation in practical implementation can be expected due to noise, PWM nonlinearities, and components imperfection. The digital simulation using MATHLAB/Simulink is performed to validate the functionality of the system.展开更多
This paper models a low-power high-frequency digitally controlled synchronous rectifier （SR） OUCK converter. The converter is a hybrid system with three operation modes. Digital PID controler is used. Key problems s...This paper models a low-power high-frequency digitally controlled synchronous rectifier （SR） OUCK converter. The converter is a hybrid system with three operation modes. Digital PID controler is used. Key problems such as quantization resolution of digital pulse-width modulation （DPWM） and steady-state limit cycles of digital control switching model power supply （SMPS） are discussed, with corresponding solutions presented. Simulation of a digital control synchronous buck is performed with a fixed-point algorithm. The results show that the described approach enables high-speed dynamic performance.展开更多
文摘BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.
基金supported by National Natural Science Foundation of China(No.51477045)
文摘Modular multilevel converters(MMCs) operate in the low modulation index region in many applications.However, when utilized at the low modulation index region, large harmonics appear in the output voltage, which degrade the performance of the MMC. To improve the harmonic characteristic in the low modulation index, the carrier dynamic overlapping switching frequency optimal pulse width modulation(CDOSFOPWM) method is proposed for the MMC here. The whole modulation index region is divided into three regions: high modulation index region, middle modulation index region, and low modulation index region. The carrier amplitude, carrier overlap ratio, and frequency of triangular carriers are chosen dynamically according to the modulation index region of the modulation signals, to achieve the optimal harmonic characteristic in the whole modulation index region and maintain the switching loss. The number of on-state submodules(SMs) can be calculated by CDOSFOPWM, and the selection of SMs is performed by a reducing switching frequency voltage balancing algorithm. Finally, the proposed method is verified by simulation and experimental results.
文摘该文以三相电压型脉宽调制(pulse width modulation,PWM)整流器为研究对象,提出一种基于新型开关表的直接功率控制(direct power control,DPC)策略。首先,根据两相旋转dq坐标系下整流器数学模型计算开关矢量对有功功率和无功功率的控制幅度,并归一化处理进行定量分析,以此为基础将传统DPC的12扇区重新划分为24扇区。然后,设计有功功率差值区间判断器,根据其输出值Cp和有功功率与无功功率滞环比较器的输出值Sp、Sq,选择当前扇区最佳开关矢量构造新型开关表,实现对有功功率的精确控制。仿真对比表明,该新型开关表不仅克服传统开关表对无功功率控制能力差的缺点,而且改善网侧电流总谐波失真,降低输出直流电压波动和有功功率与无功功率脉动。最后,搭建一台800W三相电压型PWM整流器样机,通过对比实验验证所提控制策略的正确性和有效性。
文摘Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems.
文摘Matrix converter fed motor drive is superior to pulse width modulation inverter drives since it not only provides bi-directional power flow,sinusoidal input/output currents,unity input power factor,but also allows a compact design due to the lack of DC-link capacitors for energy storage.In this paper,model and control of matrix converter fed induction motor drive system are analyzed.A combined control strategy is simplified and improved,which realizes space vector pulse width modulation of matrix converter and rotor flux oriented vector control technique for induction motor drive simultaneously.This control strategy combines the advantages of matrix converter with the good drive performance of vector control technique.Experimental results demonstrate the feasibility and effectiveness of the proposed control strategy.
文摘This paper proposes the modeling and simulation technique to analyze and design a Boost converter using generalized minimum variance method with discrete-time quasi-sliding mode to adjust the converter switch through a pulse width modulation (PWM), so as to enhance a stable output voltage. The control objective is to maintain the sensed output voltage stable, constant and equal to some constant reference voltage (8 volt) in the load resistance variation (24, 48, 240) Ω and input voltage variation (20, 24, 28) volt circumstances. This control strategy is very appropriate for the digitally controlled power converter and for the system requirement accomplishment, resulting high output voltage accuracy. The performance degradation in practical implementation can be expected due to noise, PWM nonlinearities, and components imperfection. The digital simulation using MATHLAB/Simulink is performed to validate the functionality of the system.
基金the Power Electronics Science Education Development Program of Delta Environmental & EducationFoundation (Grant No.DERO2007014)the Scientific Service of the Embassy of France in China (Grant No.K06D20)
文摘This paper models a low-power high-frequency digitally controlled synchronous rectifier （SR） OUCK converter. The converter is a hybrid system with three operation modes. Digital PID controler is used. Key problems such as quantization resolution of digital pulse-width modulation （DPWM） and steady-state limit cycles of digital control switching model power supply （SMPS） are discussed, with corresponding solutions presented. Simulation of a digital control synchronous buck is performed with a fixed-point algorithm. The results show that the described approach enables high-speed dynamic performance.
