BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs ...BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.展开更多
Converters with pulse width modulation are used for connections between the direct current （DC） and alternating current （AC） networks, e.g., in uninterrupted power supply systems, AC electromotor drives, for power...Converters with pulse width modulation are used for connections between the direct current （DC） and alternating current （AC） networks, e.g., in uninterrupted power supply systems, AC electromotor drives, for powering induction furnaces, in audio technique. Spectrum of signals sampled by pulse amplitude modulation and output voltage spectrum of the converter with pulse width modulation have similar properties. Spectrum of signals sampled by pulse amplitude modulation contains a harmonic of frequency equal to the frequency of the modulating signal and the harmonics of frequencies equal to the sum of frequency of the modulating signal and multiples of the sampling frequency. The output voltage spectrum of the converter with bipolar pulse width modulation contains harmonic of frequency equal to the frequency of the modulating signal and harmonics of frequencies equal to sum of the frequency of the modulating signal and multiples of the frequency of the carrier signal. It also contains harmonics of frequencies equal to the sum of the multiples of the frequency of the modulating signal and the multiples of the carrier signal. The comparison analysis was carried out for the harmonics of the output voltage of the converter with bipolar pulse width modulation in time domain. The dependency of the amplitudes and frequency spectrum on the wave forms of the carrier signal and modulating signal was shown. Similarity of the output voltage spectrum of the converter and signal spectrum sampled by the pulse width modulation was also shown. Key words： Output voltage converter with bipolar pulse width modulation, spectral analysis, Fourier series, carrier signal, reference signal.展开更多
Modular multilevel converters(MMCs) operate in the low modulation index region in many applications.However, when utilized at the low modulation index region, large harmonics appear in the output voltage, which degrad...Modular multilevel converters(MMCs) operate in the low modulation index region in many applications.However, when utilized at the low modulation index region, large harmonics appear in the output voltage, which degrade the performance of the MMC. To improve the harmonic characteristic in the low modulation index, the carrier dynamic overlapping switching frequency optimal pulse width modulation(CDOSFOPWM) method is proposed for the MMC here. The whole modulation index region is divided into three regions: high modulation index region, middle modulation index region, and low modulation index region. The carrier amplitude, carrier overlap ratio, and frequency of triangular carriers are chosen dynamically according to the modulation index region of the modulation signals, to achieve the optimal harmonic characteristic in the whole modulation index region and maintain the switching loss. The number of on-state submodules(SMs) can be calculated by CDOSFOPWM, and the selection of SMs is performed by a reducing switching frequency voltage balancing algorithm. Finally, the proposed method is verified by simulation and experimental results.展开更多
该文以三相电压型脉宽调制(pulse width modulation,PWM)整流器为研究对象,提出一种基于新型开关表的直接功率控制(direct power control,DPC)策略。首先,根据两相旋转dq坐标系下整流器数学模型计算开关矢量对有功功率和无功功率的控制...该文以三相电压型脉宽调制(pulse width modulation,PWM)整流器为研究对象,提出一种基于新型开关表的直接功率控制(direct power control,DPC)策略。首先,根据两相旋转dq坐标系下整流器数学模型计算开关矢量对有功功率和无功功率的控制幅度,并归一化处理进行定量分析,以此为基础将传统DPC的12扇区重新划分为24扇区。然后,设计有功功率差值区间判断器,根据其输出值Cp和有功功率与无功功率滞环比较器的输出值Sp、Sq,选择当前扇区最佳开关矢量构造新型开关表,实现对有功功率的精确控制。仿真对比表明,该新型开关表不仅克服传统开关表对无功功率控制能力差的缺点,而且改善网侧电流总谐波失真,降低输出直流电压波动和有功功率与无功功率脉动。最后,搭建一台800W三相电压型PWM整流器样机,通过对比实验验证所提控制策略的正确性和有效性。展开更多
Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for...Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems.展开更多
Instead of avoiding occurrence of chaos in motor drives, chaos is positively utilized in this paper. A new chaotic pulse width modulation （PWM） scheme is proposed and implemented for AC motors, which functions to si...Instead of avoiding occurrence of chaos in motor drives, chaos is positively utilized in this paper. A new chaotic pulse width modulation （PWM） scheme is proposed and implemented for AC motors, which functions to significantly suppress harmonic peaks and hence acoustic noise. The key is to employ the Chua＇s circuit for generating a desired chaotic sequence. By using a practical induction motor, computer simulation and experimental results verify that the chaotic PWM has advantages of harmonic peak suppression and simple hardware implementation over the conventional PWM and random PWM.展开更多
文摘BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.
文摘Converters with pulse width modulation are used for connections between the direct current （DC） and alternating current （AC） networks, e.g., in uninterrupted power supply systems, AC electromotor drives, for powering induction furnaces, in audio technique. Spectrum of signals sampled by pulse amplitude modulation and output voltage spectrum of the converter with pulse width modulation have similar properties. Spectrum of signals sampled by pulse amplitude modulation contains a harmonic of frequency equal to the frequency of the modulating signal and the harmonics of frequencies equal to the sum of frequency of the modulating signal and multiples of the sampling frequency. The output voltage spectrum of the converter with bipolar pulse width modulation contains harmonic of frequency equal to the frequency of the modulating signal and harmonics of frequencies equal to sum of the frequency of the modulating signal and multiples of the frequency of the carrier signal. It also contains harmonics of frequencies equal to the sum of the multiples of the frequency of the modulating signal and the multiples of the carrier signal. The comparison analysis was carried out for the harmonics of the output voltage of the converter with bipolar pulse width modulation in time domain. The dependency of the amplitudes and frequency spectrum on the wave forms of the carrier signal and modulating signal was shown. Similarity of the output voltage spectrum of the converter and signal spectrum sampled by the pulse width modulation was also shown. Key words： Output voltage converter with bipolar pulse width modulation, spectral analysis, Fourier series, carrier signal, reference signal.
基金supported by National Natural Science Foundation of China(No.51477045)
文摘Modular multilevel converters(MMCs) operate in the low modulation index region in many applications.However, when utilized at the low modulation index region, large harmonics appear in the output voltage, which degrade the performance of the MMC. To improve the harmonic characteristic in the low modulation index, the carrier dynamic overlapping switching frequency optimal pulse width modulation(CDOSFOPWM) method is proposed for the MMC here. The whole modulation index region is divided into three regions: high modulation index region, middle modulation index region, and low modulation index region. The carrier amplitude, carrier overlap ratio, and frequency of triangular carriers are chosen dynamically according to the modulation index region of the modulation signals, to achieve the optimal harmonic characteristic in the whole modulation index region and maintain the switching loss. The number of on-state submodules(SMs) can be calculated by CDOSFOPWM, and the selection of SMs is performed by a reducing switching frequency voltage balancing algorithm. Finally, the proposed method is verified by simulation and experimental results.
文摘该文以三相电压型脉宽调制(pulse width modulation,PWM)整流器为研究对象,提出一种基于新型开关表的直接功率控制(direct power control,DPC)策略。首先,根据两相旋转dq坐标系下整流器数学模型计算开关矢量对有功功率和无功功率的控制幅度,并归一化处理进行定量分析,以此为基础将传统DPC的12扇区重新划分为24扇区。然后,设计有功功率差值区间判断器,根据其输出值Cp和有功功率与无功功率滞环比较器的输出值Sp、Sq,选择当前扇区最佳开关矢量构造新型开关表,实现对有功功率的精确控制。仿真对比表明,该新型开关表不仅克服传统开关表对无功功率控制能力差的缺点,而且改善网侧电流总谐波失真,降低输出直流电压波动和有功功率与无功功率脉动。最后,搭建一台800W三相电压型PWM整流器样机,通过对比实验验证所提控制策略的正确性和有效性。
文摘Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems.
基金Project supported by the Shanghai Leading Academic Discipline Project (Grant No.T0103)
文摘Instead of avoiding occurrence of chaos in motor drives, chaos is positively utilized in this paper. A new chaotic pulse width modulation （PWM） scheme is proposed and implemented for AC motors, which functions to significantly suppress harmonic peaks and hence acoustic noise. The key is to employ the Chua＇s circuit for generating a desired chaotic sequence. By using a practical induction motor, computer simulation and experimental results verify that the chaotic PWM has advantages of harmonic peak suppression and simple hardware implementation over the conventional PWM and random PWM.
