In order to scientifically evaluate the values of Cucurbita moschata cultivars, main botanical characters including the initial flowering date, the first fruiting node, fruit length, fruit stem length, stem diameter, ...In order to scientifically evaluate the values of Cucurbita moschata cultivars, main botanical characters including the initial flowering date, the first fruiting node, fruit length, fruit stem length, stem diameter, internode length, the transverse and longitudinal diameters of the largest leaf, single fruit weight, flesh thickness and soluble solid content of 41 cultivars were measured for conducting diversity, correlation and cluster analysis. The results revealed that the pumpkin cultivars showed large variations in fruit stem length, single fruit weight, fruit length and flesh thickness, but small variations in initial flowering date. Significant, even highly significant correlations were found among the tested traits. Cluster analysis demonstrated that the 41 old Cucurbita moschata cultivars were divided into three groups, of which multiple traits of Group 1 were better than those in the other two groups. High similarities existed in three groups and the cultivars in each group. This research provided basis for selecting excellent traits and parents for the breeding of hybrids.展开更多
[Objective] The aim was to clone the CmNAC gene from Cucurbita moschata and analyze the sequence characteristics. [Method] A pair of degenerate primers was designed based on the conserved sequences of NAC gene from Br...[Objective] The aim was to clone the CmNAC gene from Cucurbita moschata and analyze the sequence characteristics. [Method] A pair of degenerate primers was designed based on the conserved sequences of NAC gene from Brassica napus, Lycopersicon esculentum and Capsicum annuum. NAC transcription factor gene was amplified by RT-PCR from Cucurbita moschata leaves and cloned into pMD-19T vector; then the recombinant clones were sequenced. Finally, the sequences of nucleic acid and amino acid were analyzed using BLAST and DNAMAN software. [Result] The NAC transcription factor gene cloned from C. moschata included 442 bp encoding 147 amino acids, named CmNAC. The NAC gene fragment contained a conserved region like other plant NAC genes and belonged to the NAC family ATAF1/2 subfamily. [Conclusion] The stress resistance related gene NAC cloned from C. moschata is a foundation for further study on the biological function of the gene and plant genetic engineering.展开更多
Cucurmosin 2, a type 1 ribosome-inactivating protein （RIP） isolated from sarcocarp of Cucurbita moschata, has been crystallized by the vapor-diffusion method using PEG6000 as the precipitant. The crystals belong to ...Cucurmosin 2, a type 1 ribosome-inactivating protein （RIP） isolated from sarcocarp of Cucurbita moschata, has been crystallized by the vapor-diffusion method using PEG6000 as the precipitant. The crystals belong to the orthorhombic space group P212121, with unit cell parameters a = 55.853, b = 65.507, c = 91.754 А, and have one molecule per asymmetric unit. X-ray data have been collected to 1.8А, using a synchrotron source.展开更多
Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR, such as complex operation and difficult application in production practice, in this study, a simple SSR-based method...Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR, such as complex operation and difficult application in production practice, in this study, a simple SSR-based method was established for purity identification of Cucurbita moschata hybrids. By using the established simple method, without grinding, freezing, centrifugation and drying, the genomic DNA extraction process is shorter than 3 min. Compared with the conventional method, PCR detection system and silver staining in polyacrylamide gel electrophoresis of the established method are more time-saving and cost-saving. The whole detection process is shorter than 4 h, and 480 samples can be detected with this method by one person in one day. In addition, the detection result exhibits a coincidence rate of 99% with field identification. The simple SSR-based method established in this study can provide basis for large-scale rapid purity identification of Cucurbita moschata hybrids.展开更多
为探明不同砧穗组合对南瓜[Cucurbita moschata(Duch. ex Lam.)Duch. ex Poiret]成活率、生长势、产量及果实品质的影响,指导南瓜生产,以黑杆南瓜为砧木,品沃207、奶油小王子、娃娃南瓜2K为接穗,研究不同砧穗组合南瓜的生长情况、单瓜...为探明不同砧穗组合对南瓜[Cucurbita moschata(Duch. ex Lam.)Duch. ex Poiret]成活率、生长势、产量及果实品质的影响,指导南瓜生产,以黑杆南瓜为砧木,品沃207、奶油小王子、娃娃南瓜2K为接穗,研究不同砧穗组合南瓜的生长情况、单瓜重、果实中可溶性蛋白质、淀粉、维生素C、可溶性糖含量的变化。结果表明,奶油小王子/黑杆南瓜亲和性好,与自根苗相比株高和茎粗均增长了40%以上,除可溶性蛋白质外,淀粉、可溶性糖、维生素C含量明显提高,但增产效果不明显;品沃207/黑杆南瓜亲和性好,增产达35.94%,茎粗增长明显,但可溶性蛋白质、淀粉、维生素C、可溶性糖含量提高不明显;娃娃南瓜2K/黑杆南瓜嫁接成活率仅有85.21%,亲和性较差,植株生长受限,单瓜重降低了12.97%,品质下降;因此,奶油小王子/黑杆南瓜、品沃207/黑杆南瓜2个砧穗组合嫁接效果较好,综合性状优于自根苗,可在生产中推广应用。展开更多
选取9个南瓜[Cucurbita moschata(Duchesne ex Lam.)Duchesne ex Poir.]品种进行果实品质及营养成分含量的比较,筛选出适宜山东省莱阳市种植的优质南瓜品种。结果表明,红板栗南瓜最轻,德丰银栗和红板栗南瓜的果实品质较好;甜蜜蜜红蜜南...选取9个南瓜[Cucurbita moschata(Duchesne ex Lam.)Duchesne ex Poir.]品种进行果实品质及营养成分含量的比较,筛选出适宜山东省莱阳市种植的优质南瓜品种。结果表明,红板栗南瓜最轻,德丰银栗和红板栗南瓜的果实品质较好;甜蜜蜜红蜜南瓜的维生素C含量最高,红板栗南瓜次之;德丰银栗的可溶性糖含量最高。可见,德丰银栗和红板栗南瓜在物理性状和营养成分上表现的综合品质较优,是莱阳市值得推广和发展的优良品种。展开更多
Pumpkins (Cucurbita spp.) are an important specialty vegetable. Field studies were conducted on three pumpkin cultivars characterized with different growth habits to determine the effects of plant population and genot...Pumpkins (Cucurbita spp.) are an important specialty vegetable. Field studies were conducted on three pumpkin cultivars characterized with different growth habits to determine the effects of plant population and genotypes on marketable yield. Increasing plant populations from 4780 to 9560 plant per hectare resulted in significantly greater fruit number and yield in both growing seasons and for all tested genotypes. Average fruit weight declined at the higher populations. The response of pumpkin genotypes to different planting densities was genotype (growth habit) dependent since the response was pronounced in large vine types compared to bush type. The phenotypic variation existed among pumpkin genotypes for yield seems to be under genetic control. Foliar application of potassium improved growth and yield of pumpkin plants although the non-significant effect. These results demonstrate that growers may increase pumpkin yield by increasing plant populations.展开更多
In the literature there are many reports on the composition and properties of pumpkin seed oil; however, few is known about the effect of different stages of seed development on various fatty acid profiles in developi...In the literature there are many reports on the composition and properties of pumpkin seed oil; however, few is known about the effect of different stages of seed development on various fatty acid profiles in developing seeds. The objective of this study was to provide the changes of various fatty acid accumulations in seed oil obtained from the seeds of three pumpkin varieties belonging to the species Cucurbita maxima and Cucurbita pepo. Unsaturated acids(oleic and linoleic) were dominant in various fatty acids, which constituted 38.9%-49.1% and 29.4%-42.7% of the total fatty acids at seed maturity for three pumpkin varieties, respectively, while other fatty acid concentrations except for palmitic acid all did not reach 10%. Different varieties exhibited greater effect on various fatty acid contents and the total fatty acid contents in the seeds of pumpkin rather than the species. On the whole, palmitic acid profiles of the seed oil in three varieties all followed the fluctuant decrease during all the stages of seed development, but palmitoleic acid and the total fatty acid profiles of the seed oil in three varieties were just the opposite. Stearic, oleic and linoleic acid profiles of the seed oil in three varieties all experienced the third pattern that fluctuated during all the stages of seed development, but no significant differences in these three fatty acid concentrations were found from the beginning to the end. Linolenic acid concentrations of three varieties were on the decline and ultimately close to zero. Myristic and arachidic acid profiles of the seed oil followed different trends in three varieties. Among them, myristic and arachidic acid profiles of the seed oil of Yinhui-1 fluctuated downward all the time until seed maturity, but those of 0238-1 and Jinhui-2 completely changed oppositely.展开更多
基金Supported by Special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture of China(201303112)the 12th National Five-year Plan for Science and Technology Program of Rural Areas(2012BAD02B03-17)~~
文摘In order to scientifically evaluate the values of Cucurbita moschata cultivars, main botanical characters including the initial flowering date, the first fruiting node, fruit length, fruit stem length, stem diameter, internode length, the transverse and longitudinal diameters of the largest leaf, single fruit weight, flesh thickness and soluble solid content of 41 cultivars were measured for conducting diversity, correlation and cluster analysis. The results revealed that the pumpkin cultivars showed large variations in fruit stem length, single fruit weight, fruit length and flesh thickness, but small variations in initial flowering date. Significant, even highly significant correlations were found among the tested traits. Cluster analysis demonstrated that the 41 old Cucurbita moschata cultivars were divided into three groups, of which multiple traits of Group 1 were better than those in the other two groups. High similarities existed in three groups and the cultivars in each group. This research provided basis for selecting excellent traits and parents for the breeding of hybrids.
基金Supported by Stress Resistant Plants Research Program from Beijing Municipal Commission of Education(5075101019)~~
文摘[Objective] The aim was to clone the CmNAC gene from Cucurbita moschata and analyze the sequence characteristics. [Method] A pair of degenerate primers was designed based on the conserved sequences of NAC gene from Brassica napus, Lycopersicon esculentum and Capsicum annuum. NAC transcription factor gene was amplified by RT-PCR from Cucurbita moschata leaves and cloned into pMD-19T vector; then the recombinant clones were sequenced. Finally, the sequences of nucleic acid and amino acid were analyzed using BLAST and DNAMAN software. [Result] The NAC transcription factor gene cloned from C. moschata included 442 bp encoding 147 amino acids, named CmNAC. The NAC gene fragment contained a conserved region like other plant NAC genes and belonged to the NAC family ATAF1/2 subfamily. [Conclusion] The stress resistance related gene NAC cloned from C. moschata is a foundation for further study on the biological function of the gene and plant genetic engineering.
基金supported by the National Natural Science Foundation of China (No. 39970872, 30772587)the Natural Science Foundation of Fujian Province (C97052,C0510012)+1 种基金Special Fund of Fujian Development and Reform Commission (2006-34)supported by the National Science Foundation of USA
文摘Cucurmosin 2, a type 1 ribosome-inactivating protein （RIP） isolated from sarcocarp of Cucurbita moschata, has been crystallized by the vapor-diffusion method using PEG6000 as the precipitant. The crystals belong to the orthorhombic space group P212121, with unit cell parameters a = 55.853, b = 65.507, c = 91.754 А, and have one molecule per asymmetric unit. X-ray data have been collected to 1.8А, using a synchrotron source.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest"Technology Research and Demonstration of Pumpkin Industry"(201303112)"Twelfth Five-Year"National Science and Technology Programin Rural Areas"Heterosis Utilization and New Variety Breeding of Pumpkin"(2012BAD02B03-17)
文摘Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR, such as complex operation and difficult application in production practice, in this study, a simple SSR-based method was established for purity identification of Cucurbita moschata hybrids. By using the established simple method, without grinding, freezing, centrifugation and drying, the genomic DNA extraction process is shorter than 3 min. Compared with the conventional method, PCR detection system and silver staining in polyacrylamide gel electrophoresis of the established method are more time-saving and cost-saving. The whole detection process is shorter than 4 h, and 480 samples can be detected with this method by one person in one day. In addition, the detection result exhibits a coincidence rate of 99% with field identification. The simple SSR-based method established in this study can provide basis for large-scale rapid purity identification of Cucurbita moschata hybrids.
文摘为探明不同砧穗组合对南瓜[Cucurbita moschata(Duch. ex Lam.)Duch. ex Poiret]成活率、生长势、产量及果实品质的影响,指导南瓜生产,以黑杆南瓜为砧木,品沃207、奶油小王子、娃娃南瓜2K为接穗,研究不同砧穗组合南瓜的生长情况、单瓜重、果实中可溶性蛋白质、淀粉、维生素C、可溶性糖含量的变化。结果表明,奶油小王子/黑杆南瓜亲和性好,与自根苗相比株高和茎粗均增长了40%以上,除可溶性蛋白质外,淀粉、可溶性糖、维生素C含量明显提高,但增产效果不明显;品沃207/黑杆南瓜亲和性好,增产达35.94%,茎粗增长明显,但可溶性蛋白质、淀粉、维生素C、可溶性糖含量提高不明显;娃娃南瓜2K/黑杆南瓜嫁接成活率仅有85.21%,亲和性较差,植株生长受限,单瓜重降低了12.97%,品质下降;因此,奶油小王子/黑杆南瓜、品沃207/黑杆南瓜2个砧穗组合嫁接效果较好,综合性状优于自根苗,可在生产中推广应用。
文摘选取9个南瓜[Cucurbita moschata(Duchesne ex Lam.)Duchesne ex Poir.]品种进行果实品质及营养成分含量的比较,筛选出适宜山东省莱阳市种植的优质南瓜品种。结果表明,红板栗南瓜最轻,德丰银栗和红板栗南瓜的果实品质较好;甜蜜蜜红蜜南瓜的维生素C含量最高,红板栗南瓜次之;德丰银栗的可溶性糖含量最高。可见,德丰银栗和红板栗南瓜在物理性状和营养成分上表现的综合品质较优,是莱阳市值得推广和发展的优良品种。
文摘Pumpkins (Cucurbita spp.) are an important specialty vegetable. Field studies were conducted on three pumpkin cultivars characterized with different growth habits to determine the effects of plant population and genotypes on marketable yield. Increasing plant populations from 4780 to 9560 plant per hectare resulted in significantly greater fruit number and yield in both growing seasons and for all tested genotypes. Average fruit weight declined at the higher populations. The response of pumpkin genotypes to different planting densities was genotype (growth habit) dependent since the response was pronounced in large vine types compared to bush type. The phenotypic variation existed among pumpkin genotypes for yield seems to be under genetic control. Foliar application of potassium improved growth and yield of pumpkin plants although the non-significant effect. These results demonstrate that growers may increase pumpkin yield by increasing plant populations.
基金Supported by the Public Welfare Industry(Agricultural)Research Special Foundation of Agricultural Ministry of China(201303112)
文摘In the literature there are many reports on the composition and properties of pumpkin seed oil; however, few is known about the effect of different stages of seed development on various fatty acid profiles in developing seeds. The objective of this study was to provide the changes of various fatty acid accumulations in seed oil obtained from the seeds of three pumpkin varieties belonging to the species Cucurbita maxima and Cucurbita pepo. Unsaturated acids(oleic and linoleic) were dominant in various fatty acids, which constituted 38.9%-49.1% and 29.4%-42.7% of the total fatty acids at seed maturity for three pumpkin varieties, respectively, while other fatty acid concentrations except for palmitic acid all did not reach 10%. Different varieties exhibited greater effect on various fatty acid contents and the total fatty acid contents in the seeds of pumpkin rather than the species. On the whole, palmitic acid profiles of the seed oil in three varieties all followed the fluctuant decrease during all the stages of seed development, but palmitoleic acid and the total fatty acid profiles of the seed oil in three varieties were just the opposite. Stearic, oleic and linoleic acid profiles of the seed oil in three varieties all experienced the third pattern that fluctuated during all the stages of seed development, but no significant differences in these three fatty acid concentrations were found from the beginning to the end. Linolenic acid concentrations of three varieties were on the decline and ultimately close to zero. Myristic and arachidic acid profiles of the seed oil followed different trends in three varieties. Among them, myristic and arachidic acid profiles of the seed oil of Yinhui-1 fluctuated downward all the time until seed maturity, but those of 0238-1 and Jinhui-2 completely changed oppositely.
