Red rice(Oryza punctata) is a type of unpolished rice which has higher nutritional value compared to white rice or even polished rice. Owing to higher nutritive content and metabolites, dieticians strongly advise red ...Red rice(Oryza punctata) is a type of unpolished rice which has higher nutritional value compared to white rice or even polished rice. Owing to higher nutritive content and metabolites, dieticians strongly advise red rice for peoples with metabolic disorders including diabetics. However, the mechanism of action and contents of secondary metabolites in Indian red rice variety not reported scientifically. Therefore, the present study aimed to evaluate its mechanism of action through inhibitory effect of α-amylase and α-glucosidase. Initially, the whole grain of red rice was macerated with methanol at room temperature for 2 weeks. Then, the dried and powdered, samples at different concentrationfi(2.5, 10, 40, and 80 μg/m L) were employed to nd out in vitro inhibitory effects on α-amylase and α-glucosidase. In addition, an enzyme kinetics of effective extract was calculated by Line-weaver Burk(LWB) plot analysis. Moreover,the valuable metabolites in the efficient methanolic extract were quantified using high performance liquid chromatography(HPLC). The results demonstrated that red rice methanolic extract(RRMEt) possess strong inhibitory activity onα-amylase and α-glucosidase compared with acarbose(P < 0.01). The IC50 values of RRMEt was found to be 29.7 ±7.43 μg/m L for α-amylase and 20.4 ± 0.25 μg/m L for α-glucosidase. LWB indicated that RRMEt is an uncompetitive inhibitor. Further, HPLC analysis revealed protocatechuic acid, catechin, and chlorogenic acids were more abundant in RRMEt among the fourteen metabolites. We conclude, the efficiency of enzyme inhibition through the influence of phenolic compounds in RRMEt.展开更多
A new acylated flavonoid glycoside, 3-O-kaempferol-3-O-acetyl-6-O-(p-coumaroyl)-- D-glucopyranoside 1 was isolated from the whole plant of Anaphalis aureo-punctata. The structure was established by spectral methods.
Rhizomelic Chondrodysplasia Punctata Type I is one of the rare peroxisome disorders. We report the case of a newborn white male that developed seizures and skeletal dysmorphism. The baby had short humerus bones with s...Rhizomelic Chondrodysplasia Punctata Type I is one of the rare peroxisome disorders. We report the case of a newborn white male that developed seizures and skeletal dysmorphism. The baby had short humerus bones with stippled epiphy-ses, consistent with the disease. He had also delay in myelinization on brain MRI with bilateral subependymal cysts over the atria and frontal horns of the lateral ventricles. Usually, infants with this disorder do not live long. This unfor-tunate little patient died at 5 weeks age from pneumonia. We emphasize the importance of antenatal screening for these disorders especially when a family history of dysmorphism is positive.展开更多
为研究紫竹(Phyllostachys nigra var.punctata)开花后叶片光合特性与叶绿素荧光参数的变化,以不同生长年限开花与未开花紫竹为试材,测定叶片的光合指标与叶绿素荧光参数。结果表明:蒸腾速率与净光合速率显著相关,与气孔导度极显著相关...为研究紫竹(Phyllostachys nigra var.punctata)开花后叶片光合特性与叶绿素荧光参数的变化,以不同生长年限开花与未开花紫竹为试材,测定叶片的光合指标与叶绿素荧光参数。结果表明:蒸腾速率与净光合速率显著相关,与气孔导度极显著相关;最大光合效率(Fv/Fm)与光系统II的实际光合效率[Y(II)]、表观电子传递速率(ETR)显著相关;但净光合速率与Fv/Fm相关性不显著。通过主成分分析法以光合能力强、光合速率快和强光照适应能力好为评判标准得出,3年生开花紫竹光合能力最弱、光合速率最慢、强光照适应能力最差,表明生长年限较大的紫竹开花后综合光合能力较差,其生长发育走向死亡。研究结果可为进一步探求紫竹开花的内在生理变化提供参考依据。展开更多
基金Department of Science and Technology,Government of India,for providing INSPIRE Fellowshipauthorities of Annamalai University for providing necessary support
文摘Red rice(Oryza punctata) is a type of unpolished rice which has higher nutritional value compared to white rice or even polished rice. Owing to higher nutritive content and metabolites, dieticians strongly advise red rice for peoples with metabolic disorders including diabetics. However, the mechanism of action and contents of secondary metabolites in Indian red rice variety not reported scientifically. Therefore, the present study aimed to evaluate its mechanism of action through inhibitory effect of α-amylase and α-glucosidase. Initially, the whole grain of red rice was macerated with methanol at room temperature for 2 weeks. Then, the dried and powdered, samples at different concentrationfi(2.5, 10, 40, and 80 μg/m L) were employed to nd out in vitro inhibitory effects on α-amylase and α-glucosidase. In addition, an enzyme kinetics of effective extract was calculated by Line-weaver Burk(LWB) plot analysis. Moreover,the valuable metabolites in the efficient methanolic extract were quantified using high performance liquid chromatography(HPLC). The results demonstrated that red rice methanolic extract(RRMEt) possess strong inhibitory activity onα-amylase and α-glucosidase compared with acarbose(P < 0.01). The IC50 values of RRMEt was found to be 29.7 ±7.43 μg/m L for α-amylase and 20.4 ± 0.25 μg/m L for α-glucosidase. LWB indicated that RRMEt is an uncompetitive inhibitor. Further, HPLC analysis revealed protocatechuic acid, catechin, and chlorogenic acids were more abundant in RRMEt among the fourteen metabolites. We conclude, the efficiency of enzyme inhibition through the influence of phenolic compounds in RRMEt.
文摘A new acylated flavonoid glycoside, 3-O-kaempferol-3-O-acetyl-6-O-(p-coumaroyl)-- D-glucopyranoside 1 was isolated from the whole plant of Anaphalis aureo-punctata. The structure was established by spectral methods.
文摘Rhizomelic Chondrodysplasia Punctata Type I is one of the rare peroxisome disorders. We report the case of a newborn white male that developed seizures and skeletal dysmorphism. The baby had short humerus bones with stippled epiphy-ses, consistent with the disease. He had also delay in myelinization on brain MRI with bilateral subependymal cysts over the atria and frontal horns of the lateral ventricles. Usually, infants with this disorder do not live long. This unfor-tunate little patient died at 5 weeks age from pneumonia. We emphasize the importance of antenatal screening for these disorders especially when a family history of dysmorphism is positive.
文摘为研究紫竹(Phyllostachys nigra var.punctata)开花后叶片光合特性与叶绿素荧光参数的变化,以不同生长年限开花与未开花紫竹为试材,测定叶片的光合指标与叶绿素荧光参数。结果表明:蒸腾速率与净光合速率显著相关,与气孔导度极显著相关;最大光合效率(Fv/Fm)与光系统II的实际光合效率[Y(II)]、表观电子传递速率(ETR)显著相关;但净光合速率与Fv/Fm相关性不显著。通过主成分分析法以光合能力强、光合速率快和强光照适应能力好为评判标准得出,3年生开花紫竹光合能力最弱、光合速率最慢、强光照适应能力最差,表明生长年限较大的紫竹开花后综合光合能力较差,其生长发育走向死亡。研究结果可为进一步探求紫竹开花的内在生理变化提供参考依据。
