Mapping of Purple Gene in Spears of Asparagus(Asparagus officinalis L.)

[Objectives]This study was conducted to find out regulatory genes related to purple in spears of asparagus(Asparagus officinalis L.).[Methods]The stable asparagus inbred line JX1513-5(the base of the spear is purple)and JLV1718-7(the base of the spear is green)were used as parents to study the genetic law of purple/green traits in their offspring.[Results]The results showed that the purple in the basal part of asparagus spear was controlled by a pair of alleles,and purple was dominant over green.The F 2 segregation population was resequenced by the bulk segregation analysis(BSA)method,and the purple trait in the basal part of asparagus spear was located in the interval of 24.51-25.08 Mb on Chr07 chromosome,which included 47 genes.According to the annotation information,three candidate genes were screened out:LOC109849403,LOC109849430 and LOC109849442.The candidate genes were verified by real-time fluorescence quantitative PCR(qRT-PCR),and finally LOC109849442 was obtained as the candidate gene for controlling the purple/green trait in the basal part of asparagus spear.[Conclusions]This study lays a foundation for the breeding of new asparagus varieties and molecular marker-assisted breeding.

Observation of therapeutic effect of lamp irradiation combined with purple gromwell oil gauze on alleviating intestinal colic in patients

BACKGROUND Intestinal colic is a common complication in patients who have undergone radical surgery for colorectal cancer.Traditional Chinese medicine has advantages,including safety and stability,for the treatment of intestinal colic.Lamp irra-diation for abdominal ironing has been applied in the treatment of many gas-trointestinal diseases.Purple gromwell oil has the effects of clearing heat,cooling blood,reducing swelling,and relieving pain.RESULTS The general effective rate in the observation group was 95.00%,which was significantly higher than that in the control group(86.67%,P<0.05).Before treatment,there was no significant difference in the duration of symptoms between the groups(P>0.05).After 1,2,3,and 4 d of treatment,the duration of symptoms in both groups were decreased,and the duration in the observation group was significantly lower than that in the control group(96.54±9.57 vs 110.45±11.23,87.26±12.07 vs 104.44±11.68,80.45±16.21 vs 99.44±14.95,73.18±15.58 vs 92.17±14.20;P<0.05).After 1,3,5,and 7 d of treatment,the NRS scores in both groups were decreased,and the NRS scores in the observation group were significantly lower than those in the control group(3.56±0.41 vs 4.04±0.58,3.07±0.67 vs 3.74±1.02,2.52±0.76 vs 3.43±0.85,2.03±0.58 vs 3.03±0.82;P<0.05).There was no significant difference in the rate of adverse reaction occurrence between the groups(P>0.05).CONCLUSION The use of lamp irradiation combined with purple gromwell oil gauze in patients with intestinal colic after radical surgery for colorectal cancer can reduce symptom duration,alleviate intestinal colic,and improve treatment efficacy,and this approach is safe.It is worth promoting the use of this treatment in clinical practice.

LncRNA PURPL调节miR-342-3p/PIK3R1轴对肝癌细胞恶性生物学行为的影响

目的:探究长链非编码RNA(PURPL)调节微小RNA-342-3p(miR-342-3p)/磷脂酰肌醇-3激酶调节亚基1(PIK3R1)轴对肝癌细胞恶性生物学行为的影响。方法:细胞实验:将si-NC、si-PURPL、si-PURPL+inhibitor NC、si-PURPL+miR-342-3p inhibitor分别转染至肝癌细胞Huh-7细胞并命名为si-NC组、si-PURPL组、si-PURPL+inhibitor NC组、si-PURPL+miR-342-3p inhibitor组,未做任何处理的Huh-7细胞记为NC组。双荧光素酶报告基因实验验证LncRNA PURPL、miR-342-3p、PIK3R1的关系;qRT-PCR检测人正常肝细胞系L02和人肝癌细胞系Huh-7中LncRNA PURPL、miR-342-3p表达;Western blot检测L02细胞、Huh-7细胞PIK3R1蛋白水平;MTT法检测Huh-7细胞增殖情况;流式细胞术检测Huh-7细胞凋亡率;Transwell检测Huh-7细胞侵袭数量;划痕试验测定Huh-7细胞迁移。体内实验:构建裸鼠异种移植模型,分组同细胞实验,检测肿瘤体积和肿瘤重量。结果:细胞实验结果显示:与si-NC组相比,si-PURPL组Huh-7细胞OD490值、划痕愈合率、侵袭细胞数量、LncRNA PURPL、PIK3R1水平显著下降(P<0.05),Huh-7细胞凋亡率、miR-342-3p相对表达量显著升高(P<0.05),而抑制miR-342-3p表达减弱了沉默LncRNA PURPL抑制Huh-7细胞增殖、迁移、侵袭和促进凋亡的效果;LncRNA PURPL负向调控miR-342-3p/PIK3R1轴。体内结果显示:si-PURPL组裸鼠肿瘤体积和质量较si-NC组下降(P<0.05);抑制miR-342-3p表达减弱了沉默LncRNA PURPL对体内肿瘤生长的抑制作用。结论:沉默LncRNA PURPL可抑制Huh-7细胞的恶性生物学行为,其机制可能与调控miR-342-3p/PIK3R1轴有关。

长链非编码RNA PURPL调控miRNA/mRNA网络的生物信息学分析

目的充分挖掘生物信息学数据库,筛选可与长链非编码RNA(long non-coding RNA,lncRNA)PURPL结合的微小RNA(microRNA,miRNA)及下游靶基因和信号通路,进而构建PURPL/miRNA/靶基因/信号通路调控网络,为后续研究PURPL参与卵巢癌细胞侵袭和转移的分子机制奠定基础。方法基于lncRNA/miRNA调控机制,多数据库交叉预测可与PURPL结合的下游miRNA及其靶基因,并对靶基因进行蛋白质互作网络分析和核心蛋白筛选,进而行基因本体(gene ontology,GO)、疾病和京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析。结果PURPL在正常组织和癌组织(包括正常卵巢组织和卵巢癌组织)中存在差异化表达,筛选出3个下游miRNA及其341个靶基因,经过蛋白质互作网络分析、富集分析,筛选出15个核心基因,最终确定了PURPL、下游3个miRNA、15个核心靶基因及潜在信号通路组成的调控网络。结论PURPL/miRNA/靶基因/信号通路调控网络可为后续卵巢癌侵袭和转移的分子机制研究提供潜在的分子靶点。

Use of Agent Purple, Agent Orange and Agent Blue on Royal Thai Air Force Base Perimeters in Thailand during the Vietnam War

Seven Royal Thai Air Force bases in Thailand were used by the United States Air Force (USAF) during the Vietnam War as staging hubs for operations in Laos and Cambodia. Five bases in Thailand, including Nakhon Phanom (NKP), Ubon, Korat, U-Tapao and Udorn endured sniper fire interdiction, perimeter penetration, and sapper (combat engineer) attacks. Nam Phong, an eighth Royal Thai Air Force base was used by the United States Marine Corps air operations starting in 1972. US Military personnel stationed throughout Thailand were also attacked by Communists insurgents. Two herbicides, Agent Purple and Agent Orange containing 2, 4, 5-T contaminated with dioxin (TCDD—2, 3, 7, 8 tetrachlorodibenzodioxin) and a third herbicide, the arsenic-based Agent Blue, were routinely received at these Thailand airbases in support of air missions and to keep airbases and perimeter fences clear of vegetation. Udorn Royal Thai Air Force (RTAF) base, established in the 1950s was consistently a target of enemy attacks during this period. The Thai government allowed the United States to use five bases, covertly, and two other Thai bases, openly, due to concerns that the civil war inside Laos might spread into Thailand. The US Air Force began, in 1961, to provide the air defense of Thailand and to fly reconnaissance flights over Laos. Under the United States and Thailand’s “gentleman’s agreement”, the bases used by the USAF were considered RTAF bases under the command of Thai officers. The USAF at Udorn was under the command of the United States Pacific Air Forces (PACAF) Thirteenth Air Force and was used to temporarily store and distribute Agent Purple, Agent Orange and Agent Blue to Laos’s airfields for spraying of the Ho Chi Minh Trail. Thai air police controlled access to the bases using sentry dogs, observation towers, and machine gun emplacements. The USAF Security police assisted the Thai air police in base defense. In this study, we document the use of Agent Orange, Agent Purple and Agent Blue on Royal Thai Air Force base perimeters and grounds during the Vietnam War, potential active-duty service personnel exposure to these toxic herbicides and health impacts of the contaminant dioxin TCDD and arsenic on U.S. Vietnam Era Veterans and Vietnam Veterans. This documentation is important evidence in the “assumption of exposure” for health claims to the US Veterans Administration (VA) by veterans that served in Thailand between 1962 and 1976.

Effects of Combined Application of Biochar-based Organic Fertilizer and Reduced Nitrogen Fertilizer on Soil Enzyme Activity and Yield of Purple Cabbage(Brassica oleracea var.capita rubra)in Yuanmou County

[Objectives]In response to the issue of soil improvement in Yuanmou County,the effects of combined application of biochar-based organic fertilizer and reduced nitrogen fertilizer on soil nutrients,soil enzyme activity,and yield of purple cabbage(Brassica oleracea var.capita rubra)were investigated in the field base of Institute of Thermal Zone Ecological Agriculture,Yunnan Academy of Agricultural Sciences in Yuanmou County.[Methods]A total of 13 treatments were set up by applying biochar-based organic fertilizer at three levels of 15,30 and 45 t/hm^(2)(T_(1),T_(2),T_(3)),combined with top application of nitrogen fertilizer(urea)at four levels:375(N_1),300(N_(2)),225(N_(3))and 0 kg/hm^(2),with non-fertilizing treatment as control check(CK),in order to explore the optimal ratio for the combined application of biochar-based organic fertilizer with nitrogen fertilizer.[Results]The application of biochar-based organic fertilizer could significantly improve soil nutrients,enzyme activity,and purple cabbage yield.The improvement effect of combined application with nitrogen fertilizer was higher than that of single application of biochar-based organic fertilizer,and the improvement effect was enhanced with the application amount of biochar-based organic fertilizer increasing.The contents of organic matter and total nitrogen were the highest in treatment T_(3)N_(3),of which the values increased by 81.39%and 56.09%compared with the CK,respectively.The contents of soil hydrolyzable nitrogen,available phosphorus,and available potassium were all the highest under treatment T_(3)N_(2),with increases of 92.76%,171.01%and 235.50%,respectively.There was a significant positive correlation between the activity of soil catalase,urease,and sucrase and organic matter,total nitrogen,and available nutrients.The overall soil enzyme activity was relatively higher in treatment T_(3)N_(2).The yield of purple cabbage treated with biochar-based organic fertilizer combined with nitrogen fertilizer could reach 85750 kg/hm^(2),which was 94.78%higher than that treated with biochar-based organic fertilizer alone.Based on comprehensive analysis,the optimal combination ratio was 45 t/hm^(2)of biochar-based organic fertilizer and 300 kg/hm^(2)of urea(T_(3)N_(2)).[Conclusions]This study provides data support for the promotion of biochar-based organic fertilizers and reduced fertilizer in agricultural soil in the Dam area of Yuanmou County.

Effects of Long-term Fertilization on Micro-morphological Features in Purple Soil

[Objective] The study aimed to better understand the effect of different fertilizer treatments on micro-morphological characteristics of a purple soil at the 0-20 cm topsoil in a long-term fertilizer experiment. [Method] Soil micro-morphology was observed and analyzed under a single polarizing microscope. [Result] For the CK （no fertilizer） treatment, soil structure was dense with little porosity developed. Its soil microstructure was poor, sandy fabric-granular fabric. After continuously applied chemical fertilizers only for more than two decades, the soil particles did not evolve into soil structures and formed little porosity. The microstructures of soil in N, NP and NPK treatments were porphyroskelic fabric-fine sandy granular fabric, better than that of the soil in CK treatment. Adding manure obviously improved the quantity of groundmass and endowed the soil a loose structure and plenty porosity, enriched animal and plant residues, and well-formed iron-manganese nodules and humus ma- terials, all resulting in better micro-aggregates development. The type of soil microstructures in MNPK treatment was pectized-compacted takyric fabric-intertextic fabric, the best among all the treatments. [Conclusion] Combined application of both or- ganic and inorganic fertilizers can significantly improve the structure of the purple soil, enhance soil fertility and achieve soil sustainable development.

Optimized Ultrasound-assisted Extraction of Proacyanidins from Purple Cabbage

[Objective] The paper was to optimize ultrasound-assisted extraction of proacyanidins from purple cabbage. [Method] The ultrasound-assisted extraction technology was used to study the extraction of proacyanidins from purple cabbage. The effect of ethanol concentration, temperature, time, material-solution ratio and the power of ultrasound on extracting efficiency were studied by single-factor experiment. Based on this, L16（4%orthogonal test were conducted to determine the optimal condi- tion for extraction of proacyanidins from purple cabbage. [Result] The optimal condi- tion for extraction of proacyanidins from purple cabbage was as follows： ethanol concentration 50%, extraction temperature 50℃, extraction time 1 h, extraction power 540 W, material-liquid ratio 1：20. The extraction rate of procyanidins under this condition reached 104.8 mg/g. [Conclusion] The study provided a theoretical basis for scientific and reasonable utilization of procyanidins in purple cabbage.

Influence of Replacing Bacterial Pheophytin with Pheophytin on the Picosecond Fluorescence from Reaction Centers of Purple Bacteria

Fluorescence spectra of native purple bacterial reaction center (RC) and bacterial pheophytin (Bphe) replaced RCs were obtained from 600 nm to 900 nm with a selective excitation at 597 nm. With the help of measuring the fluorescence from bacterial chlorophyll, bacterial pheophytin and plant pheophytin, the corresponding components in the RCs are classified for fluorescence emission. Results showed that pheophytin substitution influences the composition of fluorescence spectra. Therefore, four, three and two components were obtained from fluorescence spectra of native RC, Bphe B_replaced RC and Bphe A,B _replaced RC, respectively. Fluorescence components are well correlated to the binding of plant pheophytin. The decay of excited state of primary electron donor P in different RCs was also studied by measuring the fluorescence decay at 686.4, 674.1 and 681.1 nm, respectively. The decaying kinetics changed in different RCs, indicating that pheophytin replacement influenced the energy transduction and primary photochemical reaction in purple bacterial reaction centers.

Effects of Transplanting Density on Yield Formation Characteristics of Purple Rice

Liangtianzi 1 was used as a material to study the effects of transplanting density on the yield formation characteristics of purple rice. The results showed that the whole growth period of Liangtianzi 1 was 146 d. Its biomass yield was above 10 t/hm^2, while its economic yield was only about 3 t/hm^2. Transplanting density had a large impact on the number of effective panicles, number of grains par panicle, seed setting rate and yield of purple rice variety. In this study, the planting spacing of 20.0 cm × 20.0 cm gained the highest yield, 3.63 t/hm^2. Key techniques for high-yielding cultivation of Liangtianzi 1 remain to further research.

Effect of Purple Yam(Dioscorea alata L.) on Nutritional and Physiological Functions of Growing Rats

[Objective] This study was to explore the effect of purple yam on the nutritional and physiological functions of rats.[Method] Forty SD rats were randomly divided into four groups with ten rats in each group which were reared with artificial semisynthetic feed added with 10% steamed yam powder.The four groups,the casein（CK） group which was used as the control,the purple yam（PY） group,the Tiegun yam（TY） group and common Huai yam（HY） group,were supplied with feed of equal content of energy,protein and fat.The rats were pair-fed for 56 days to observe changes of relevant nutritional and physiological indices.[Result] There were no significant differences of body weight,food conversion rate and organ indices between rats in PY group and CK group.Mean corpuscular hemoglobin in PY group was significantly lower than that in CK group;hemoglobin showed no significant difference with that in CK group;eosnophils was lower than that in CK group.Low density lipoprotein-cholesterol,total cholesterol and atherogenic index in PY group were lower than that in the other three groups;anti-atherogenic index was the highest among the four groups,while showing no significant difference;triglyceride content was lower than that in CK group.Among the antioxidant indices,the activity of catalase and superoxide dismutase were higher than that in CK group,while malondialdehyde content was lower than that in CK group.[Conclusion] Purple yam showed the trend of reducing the content of blood glucose,total cholesterol,low density lipoprotein-cholesterol,triglyceride,malondialdehyde,and raising the activity of glutathione peroxidase,superoxide dismutase and catalase,thus having certain antioxidative function.

Extraction Characteristics of Anthocyanins from Preliminarily-processed Products of Purple Sweet Potatoes during the Gelatinization Process

Objective] This study aimed to investigate the method for efficient utilization and development of purple sweet potatoes. [Method] Purple sweet potatoes were dried at two specific temperatures and prepared into preliminarily-processed products for gelatinization simulation to analyze the extraction amount of anthocyanins from gelatinized samples at different gelatinization stages. [Result] During the gelatinization process, the extraction rate of anthocyanins from purple sweet potato samples reached the highest as the temperature rised from 90 ℃ to 95 ℃,and the extraction amount of anthocyanins reached the maximum at 15 min postheat preservation at 95 ℃. Purple sweet potato samples dried at 60 ℃ exhibited larger retention amount, larger maximum extraction amount and higher maximum extraction rate of anthocyanins compared with those dried at 110 ℃. [Conclusion] Drying at low temperatures and appropriately shortening the initial gelatinization stage below 90 ℃ is conducive to the retention and extraction of anthocyanins from purple sweet potatoes.

Breeding and Mechanized Cultivation of the New Bulk Purple Yam Variety Suyu 2

The new bulk purple yam variety Suyu 2,which had high quality,efficiency and was suitable for production in a highly mechanized cultivation,was bred by special and was suitable for whole-course mechanized cultivation.Integrated with agricultural machinery and agricultural trait,four kinds of machines produced by two factories from Jiangsu and Shandong could complete 10 kinds of main production processes,including rotary tillage,ridging,sowing,intertillage,topdressing,drug delivery,weeding,hilling,vine cutting and harvest.The work efficiency of single machine was 4-5 hm^2/d,and the production pattern got the cost saving and effectiveness increasing in the main yam producing area.The benefit reached over RMB 37500 yuan/hm^2 in special vegetable area.It was simple,feasible and easy to popularize the mechanization cultivation technique.

翻译与性禁忌--以The Color Purple的汉译本为例

性描写是许多经典文学作品的重要组成部分。但在文学翻译中,这样的描写往往被删除或做淡化处理,而且这样的处理方法还得到了翻译学者们的支持,理由是我国传统的性伦理容忍不了这些描写。可是在中国文学中,类似的性描写早已屡见不鲜,其艺术价值也得到了普遍的肯定。对The Color Purple的两个汉译本的分析表明,译者或出版商对性伦理的理解并不正确。他们对性描写加以特殊处理,是出于对性禁忌的顾虑。

Screening and Evaluation of Different Purple Sweet Potato Varieties Specially Used for Processing

Objective] This study almed to screen high-quality and high-yield purpIe sweet potato varieties special y used for processing which were suitabIe for pIanting in Liancheng County. [Method] By using a randomized bIock design, with ‘Longyan 7-3’ as a controI, five purpIe sweet potato varieties were introduced in the autumn of 2012 for comparaton test to evaluate agronomic tralts, economic characters, nu-tritional quality, and processing quantity of dried sweet potato products and fried chips. [Result] ‘Weixiang No.1’ exhibited the highest yield of 41.9 t/hm2, which was improved by 25.8% compared with ‘Longyan 7-3’; fresh tuber yield of ‘Jishu No. 18’ reached 31.6 t/hm2, which was simiIar to that of ‘Longyan 7-3’; dried sweet potato products of ‘Jishu No.18’ and ‘Weixiang No.1’ not onIy had simiIar process stabiIity, taste and coIor to ‘Longyan 7-3’, but also exhibited exceI ent morphoIogy, coIor, transparency, taste and texture, which indicated that these two purpIe sweet potato varieties were suitabIe for processing dried sweet potato products; fried sweet potato chips of ‘Jishu No.18’ and ‘Weixiang No.1’ had moderate oiI content, high expansion degree and simiIar quality to ‘Longyan 7-3’, which indicated that these two purpIe sweet potato varieties were suitabIe for processing fried sweet potato chips; ‘Fushu No.9’, ‘Quanzi No.1’ and ‘Ninzi No.1’ exhibited beautifuI shape, high nutritional quality and good eating quality, which were suitabIe for fresh eating or processing sweet potato powder. [Conclusion] This study provided a scientific ba-sis for Iarge-scale popuIarization and appIication of new purpIe sweet potato vari-eties special y used for processing.

Investigation on the Appropriate Dose of ^(60)Co-γ Irradiation for Tubers of Purple Sweet Potato

[Objective] This study aimed to investigate the appropriate dose of 60^Co-γ irradiation for tubers of purple sweet potato.[Methods] The tubers of two purple sweet potato varieties Xuzi L-7 and Yuenanzi were used as experimental materials,and treated with 60^Co-γ irradiation at different doses（0,25,50,75,100,125 and 150 Gy） to determine the emergence rate,number of emerged seedlings per tuber and fresh weight per seedling.[Results] After the 60^Co-γ irradiation at different doses,the emergence period of tubers was postponed while the emergence rate,number of emerged seedlings per tuber and fresh weight per plant were decreased,and the differences between different varieties and different irradiation doses reached significance level.Compared with the control,the emergence period of tubers at irradiation doses above 25 Gy were postponed by 2,3,5,6 and 12 d,respectively.[Conclusion] The half lethal dose（LD50） of Xuzi L-7 was 66.73 Gy,and that of Yuenanzi was 74.96 Gy.

Comparative Study on Seed Traits of Purple Flower Platycodon grandiflorum and White Flower Platycodon grandiflorum

[ Objective] The experiment aimed to carry out investigation and analysis on seed traits of white flower Platycodon grandiflorum and purple flower Platycodon grandif/orum. [Method] 13 white flower Plalycodon grandiflorumand purple flower Platycodon grandiflorumfrom China and foreign countrias were taken as research objects. Their seed traits such as seed length, seed width, thickness of seed and 1 000-grain weight were comparatively analyzed. [ Result] The average seed length, average seed width, average seed thickness and average 1 000-grain weight of white flower Platycodon grandiflorum were all smaller than these of purple flower Platycodon grandiflorum. The seed length of Korean white flower Platycodon grandiflorum was relatively longer and seed lengths of other white flower Platycodon grandiflorum were all shorter than these of purple flower Platycodon grandiflorum. The seed widths of white flower Platycodon grandiflorum were smaller than these of purple flower P/atycodon grandiflorum, except these of Korean white flower Platycodon grandiflorum, Heilongjiang Ning＇an and South Korean Jiangyuan. The 1 000-grain weights of white flower Platycodon grandiflorum were all smaller than these of purple flower Platycodon grandiflorum, except these of Korean Rajin and Sancheng white flower Platycodon grandiflorum. [ Conclusion] The result of this experiment provided reference for rational utilizations of purple flower Platycodon grandiflorum and white flower Platycodon grandiflorum.

Prediction Model of Secondary Substances in Anthocyanins Synthesis of Purple Corn

The aim of this study was to assay the polyphenols,flavonoid,polyphenol oxidase and phenylalnine ammonialyase which were relative to the anthocyanins synthesis of purple corn. The optimization of multiple linear regression model of anthocyanins synthesis was y=4.383 86-0.205 45x1＋5.479 638x2＋0.195 575x4. According to standard partial regression coefficient testing,the result indicated that polyphenols content was negatively correlated with anthocyanins and the relative influence to anthocyanins synthesis was-42.7%; flavonoid content and activity of polyphenol oxidase were positively correlated with anthocyanins of purple corn and the relative influence to anthocyanins synthesis were 71.45% and 73.32% respectively. There was no positive correlation between the activity of phenylalnine ammonialyase and anthocyanins of purple corn. The establishment of multiple linear regression model of anthocyanins synthesis was to provide theory foundation of producing anthocyanins in laboratory.

Quality Research on the Purple Leaf Mutant of Rice——PLM

[Objective] The aim was to carry out the quality research on a purple leaf mutant （PLM） of rice and provide the basis for applied research of purple rice.[Method] A newly discovered purple mutant of rice and its hybrid filial generations （F1 and F2） were employed as the experimental materials to determine its characteristic indexes,such as grain type,chalky grain rate,chalkiness,1 000-grain weight,brown rice percentage,protein content,amylose content,gelatinization temperature and consistency.[Result] The grain type and brown rice percentage of the parent （pro-Z） both reached standard of Ⅰ Grade,while chalky grain rate,chalkiness,amylose content and consistency did not meet the requirements of the standard.The F2 generation displayed some optimized properties,including larger grain,lower amylose content,reduced chalkiness,lower chalky grain rate and softened consistency.[Conclusion] The majority of the characteristic indexes of pro-Z did not meet the requirements of standard,but the qualities of F2 generation were all optimized to some extent.

Black Women Oppressed by the Three Giant Mountains-Reinterpretation of The Color Purple

From the perspective of black feminism, the feminist consciousness in the novel The Color Purple can be disclosed utterly that Sofia, the supporting role, faces the oppression of the three Giant Mountains, namely, the whites, the black males, and the black females.