Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and ant...Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and antiviral agents now. It also decreases bloodsugar, enhances immunity and adjusts blood lipids. The anticancer and antifungal effects ofPortulaca oleracea L. were reported in several patents. But no active component has been found. Inthis study, the chemical compositions from Portulaca oleracea L. were investigated to provideevidence for the activity of Portulaca oleracea L. .展开更多
目的基于网络药理学及分子对接技术探讨马齿苋改善肝损伤的有效活性成分及分子机制。方法通过Traditional Chinese Medicine Systems Pharmacology(TCMSP)数据库检索马齿苋的活性化合物及相关靶点;GeneCards、On⁃line Mendelian Inherit...目的基于网络药理学及分子对接技术探讨马齿苋改善肝损伤的有效活性成分及分子机制。方法通过Traditional Chinese Medicine Systems Pharmacology(TCMSP)数据库检索马齿苋的活性化合物及相关靶点;GeneCards、On⁃line Mendelian Inheritance in Man(OMIM)数据库收集肝损伤的相关靶点;通过Venny 2.1.0网站筛选马齿苋与肝损伤的共表达靶点。运用Cytoscape 3.9.1软件和Search Tool for the Retrieval of Interacting Genes/Proteins数据库构建马齿苋活性成分与肝损伤共表达靶点的“药物-主要活性成分-共表达靶点-疾病”网络以及蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络图。通过the Database for Annotation,Visu⁃alization and Integrated Discovery数据库对共表达靶点进行基因本体论(gene ontology,GO)富集和京都基因与基因组百科全书(Kyoto encycloppedia genes and genomes,KEGG)通路注释分析,阐述马齿苋改善肝损伤的作用通路及分子作用机制。通过CB-Dock在线分子对接网站验证马齿苋有效活性成分与关键靶点之间的关系。结果从TCMSP数据库中筛选出10种马齿苋活性化合物及其对应的78个靶点,从GeneCards和OMIM数据库中筛选出1489个肝损伤的相关靶点,通过Venny 2.1.0网站找到54个马齿苋活性成分与肝损伤的共表达靶点。通过Cytoscape 3.9.1软件获得“药物-主要活性成分-共表达靶点-疾病”网络并找出马齿苋改善肝损伤的主要活性成分,在PPI网络图中得到关键靶点为TNF、IL6、IL1B、MMP9、CASP3、IL10、PPARG。GO富集分析结果显示马齿苋主要对转录、凋亡等生物过程起调节作用,通过KEGG通路分析筛选出与马齿苋改善肝损伤关系密切的通路为Non-alcoholic fatty liver disease通路,并且其重要的靶点有TGFB1,INSR,TNF,TNFRSF1A,IL1A,CASP7,IL6,MAPK8,CASP8,IL1B,CASP3,PPARG和PPARA。分子对接结果表明,马齿苋中主要活性成分β-胡萝卜素、槲皮素与核心靶点TNF、CASP3、PPARG稳定结合。结论马齿苋中的β-胡萝卜素、槲皮素通过与TNF、CASP3、PPARG结合调节Non-alcoholic fatty liver disease等信号通路发挥改善肝损伤的作用。展开更多
[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw mate...[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw material.[Method] The insecticidal activities of the ethanol extracts and 5 kinds of extracts with petroleum ether,chloroform,ethyl acetate,n-butanol,water from Portulaca oleracea L..against Aphis sp.was studied;Leaves are using drug treatments of 5 extracts anti-feedant activity of Aphis sp.[Result] The results showed that 95% ethanol extract displayed high contact activity to Aphis sp.The corrected mortality after 48 h reached 95.4 % under the concentration of 50.00 mg·ml-1 and the insecticidal activities of 5 extracts against Aphis sp.was in the order that petroleum ether > chloroform > n-butanol > ethyl acetate >water.Five kinds of anti-feeding activity in the extract of the order for the water> ethyl acetate> n-butanol > chloroform >petroleum ether.[Conclusion] The extract from Portulaca oleracea L.has a strong contact toxicity of pesticide active on Aphis sp.substances and for a small polar compounds,the strong anti-feeding activity is a kind of larger polar compounds.展开更多
Purslane, a common weed, has been used as food or folk medicine in many countries. The growth, medicinal components and nutrient contents of the plant are closely associated with endophytes, especially endophytic fung...Purslane, a common weed, has been used as food or folk medicine in many countries. The growth, medicinal components and nutrient contents of the plant are closely associated with endophytes, especially endophytic fungi. In this study, the endophytic fungi associated with purslane were isolated, and the effects of the isolates on the host were investigated to lay a foundation for further research and development of purslane resources. The results showed that a total of eight endophytic fungi were isolated from purslane (collected from Hohhot, Inner Mongolia, China), and they belonged to the genera Penicillium (isolates K, N, P, M and I), Chaetomium (isolate J), Fusarium (isolate H) and Petriella (isolate O). Moreover, the growth of purslane was significantly influenced by its endophytic fungi. Isolate M can significantly decrease the germination rate, while J can significantly increase the germination rate of purslane. In addition, H, J and M can significantly increase the bud length of purslane, and the fermentation broth of P has a negative influence on the bud length of purslane. M and I can significantly increase the height, fresh weight and chlorophyll content of purslane due, in part, to the lower pH of the fermentation broth of I and M.展开更多
With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oler...With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s.展开更多
文摘Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and antiviral agents now. It also decreases bloodsugar, enhances immunity and adjusts blood lipids. The anticancer and antifungal effects ofPortulaca oleracea L. were reported in several patents. But no active component has been found. Inthis study, the chemical compositions from Portulaca oleracea L. were investigated to provideevidence for the activity of Portulaca oleracea L. .
文摘目的基于网络药理学及分子对接技术探讨马齿苋改善肝损伤的有效活性成分及分子机制。方法通过Traditional Chinese Medicine Systems Pharmacology(TCMSP)数据库检索马齿苋的活性化合物及相关靶点;GeneCards、On⁃line Mendelian Inheritance in Man(OMIM)数据库收集肝损伤的相关靶点;通过Venny 2.1.0网站筛选马齿苋与肝损伤的共表达靶点。运用Cytoscape 3.9.1软件和Search Tool for the Retrieval of Interacting Genes/Proteins数据库构建马齿苋活性成分与肝损伤共表达靶点的“药物-主要活性成分-共表达靶点-疾病”网络以及蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络图。通过the Database for Annotation,Visu⁃alization and Integrated Discovery数据库对共表达靶点进行基因本体论(gene ontology,GO)富集和京都基因与基因组百科全书(Kyoto encycloppedia genes and genomes,KEGG)通路注释分析,阐述马齿苋改善肝损伤的作用通路及分子作用机制。通过CB-Dock在线分子对接网站验证马齿苋有效活性成分与关键靶点之间的关系。结果从TCMSP数据库中筛选出10种马齿苋活性化合物及其对应的78个靶点,从GeneCards和OMIM数据库中筛选出1489个肝损伤的相关靶点,通过Venny 2.1.0网站找到54个马齿苋活性成分与肝损伤的共表达靶点。通过Cytoscape 3.9.1软件获得“药物-主要活性成分-共表达靶点-疾病”网络并找出马齿苋改善肝损伤的主要活性成分,在PPI网络图中得到关键靶点为TNF、IL6、IL1B、MMP9、CASP3、IL10、PPARG。GO富集分析结果显示马齿苋主要对转录、凋亡等生物过程起调节作用,通过KEGG通路分析筛选出与马齿苋改善肝损伤关系密切的通路为Non-alcoholic fatty liver disease通路,并且其重要的靶点有TGFB1,INSR,TNF,TNFRSF1A,IL1A,CASP7,IL6,MAPK8,CASP8,IL1B,CASP3,PPARG和PPARA。分子对接结果表明,马齿苋中主要活性成分β-胡萝卜素、槲皮素与核心靶点TNF、CASP3、PPARG稳定结合。结论马齿苋中的β-胡萝卜素、槲皮素通过与TNF、CASP3、PPARG结合调节Non-alcoholic fatty liver disease等信号通路发挥改善肝损伤的作用。
文摘[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw material.[Method] The insecticidal activities of the ethanol extracts and 5 kinds of extracts with petroleum ether,chloroform,ethyl acetate,n-butanol,water from Portulaca oleracea L..against Aphis sp.was studied;Leaves are using drug treatments of 5 extracts anti-feedant activity of Aphis sp.[Result] The results showed that 95% ethanol extract displayed high contact activity to Aphis sp.The corrected mortality after 48 h reached 95.4 % under the concentration of 50.00 mg·ml-1 and the insecticidal activities of 5 extracts against Aphis sp.was in the order that petroleum ether > chloroform > n-butanol > ethyl acetate >water.Five kinds of anti-feeding activity in the extract of the order for the water> ethyl acetate> n-butanol > chloroform >petroleum ether.[Conclusion] The extract from Portulaca oleracea L.has a strong contact toxicity of pesticide active on Aphis sp.substances and for a small polar compounds,the strong anti-feeding activity is a kind of larger polar compounds.
文摘Purslane, a common weed, has been used as food or folk medicine in many countries. The growth, medicinal components and nutrient contents of the plant are closely associated with endophytes, especially endophytic fungi. In this study, the endophytic fungi associated with purslane were isolated, and the effects of the isolates on the host were investigated to lay a foundation for further research and development of purslane resources. The results showed that a total of eight endophytic fungi were isolated from purslane (collected from Hohhot, Inner Mongolia, China), and they belonged to the genera Penicillium (isolates K, N, P, M and I), Chaetomium (isolate J), Fusarium (isolate H) and Petriella (isolate O). Moreover, the growth of purslane was significantly influenced by its endophytic fungi. Isolate M can significantly decrease the germination rate, while J can significantly increase the germination rate of purslane. In addition, H, J and M can significantly increase the bud length of purslane, and the fermentation broth of P has a negative influence on the bud length of purslane. M and I can significantly increase the height, fresh weight and chlorophyll content of purslane due, in part, to the lower pH of the fermentation broth of I and M.
文摘With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s.
