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两种新的push-pull平衡的大数据量无线传感器网络数据分发算法 被引量:5
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作者 陶孜谨 龚正虎 卢泽新 《计算机研究与发展》 EI CSCD 北大核心 2008年第7期1115-1125,共11页
无线传感器网络中如何获得较低的通信代价同时在事件数据的push和pull之间实现更好的平衡是各种数据分发算法共同追求的目标.分析了目前已公认较好的两种典型的有结构和无结构的数据分发算法,指出了它们的优缺点.在此基础上,结合这两种... 无线传感器网络中如何获得较低的通信代价同时在事件数据的push和pull之间实现更好的平衡是各种数据分发算法共同追求的目标.分析了目前已公认较好的两种典型的有结构和无结构的数据分发算法,指出了它们的优缺点.在此基础上,结合这两种算法使用的push-pull策略,针对不同应用环境下的无线传感器网络的ALL型查询的特定需求,提出了两种基于有结构和无结构存储模式相结合的混合型数据分发算法,分别是Hybrid-Dcs-Cn1(HDC1)算法和Hybrid-Dcs-Cn2(HDC2)算法.分析表明这两种算法在保证push-pull之间平衡的前提下解决了已有算法存在的热点问题:存储拷贝数多和查询性能低,能更好地适应传感器网络的特点,是两种能量高效的数据分发算法. 展开更多
关键词 数据分发 push-pull平衡 热点 通信代价 负载均衡 无线传感器网络
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Push-Pull型pFLASH开关单元结构设计及特性
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作者 刘国柱 洪根深 +5 位作者 于宗光 赵文彬 曹利超 吴素贞 李燕妃 李冰 《半导体技术》 CAS CSCD 北大核心 2018年第9期652-658,663,共8页
基于90 nm eFLASH工艺设计并制备了一种新型抗辐照Push-Pull型pFLASH开关单元,并对其特性进行了研究。该结构由2个2T-FLASH管(T1/T2)和1个信号传输PMOS管(T3)组成,采用带带隧穿(BTBT)编程方式和福勒-诺德海姆(FN)擦除方式实现... 基于90 nm eFLASH工艺设计并制备了一种新型抗辐照Push-Pull型pFLASH开关单元,并对其特性进行了研究。该结构由2个2T-FLASH管(T1/T2)和1个信号传输PMOS管(T3)组成,采用带带隧穿(BTBT)编程方式和福勒-诺德海姆(FN)擦除方式实现其"开/关"态功能。同时,对其"开/关"态特性进行表征,研究其耐久性和电荷保持特性,最后,对其抗总剂量(TID)能力进行评估。实验结果表明:该器件的"T1编程-T2擦除"与"T1擦除-T2编程"态均可以实现信号传输管的"开/关"态功能,其阈值窗口的均值约为10.5 V;在工作电压为-1.2 V条件下,T3管的"开"态驱动电流均值约为0.92 mA,"关"态漏电流低于40 pA,且均表现出了良好的一致性。同时,该器件的循环擦/写次数可达10 000次,在25℃的"开/关"态应力条件下寿命大于10年,抗总剂量能力可达150 krad(Si)以上。 展开更多
关键词 push-pull型pFLASH开关单元 “开/关”态 阈值窗口 电荷保持性 总剂量(TID)
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基于第三方的Push-Pull逆向物流模式
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作者 于建红 鲁耀斌 《管理学报》 2005年第z1期174-177,共4页
首先阐述了逆向物流的内涵、功能以及基于第三方逆向物流的提出,其次分析了外包逆向物流的优势,最后分析了生产控制系统中的Push-Pull方法.在此基础上,提出基于第三方的Push-Pull逆向物流模式.
关键词 逆向物流 第三方逆向物流 push-pull
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基于DTN网络的PUSH-PULL策略研究
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作者 崔永锋 《河南师范大学学报(自然科学版)》 CAS CSCD 北大核心 2010年第6期54-56,共3页
DTN网络是新一代的网络体系结构,PUSH-PULL是解决DTN网络中拥塞控制的一种有效策略,介绍了DTN网络中拥塞控制的研究现状,并对PUSH-PULL的各个策略进行了阐述,通过NS2搭建DTN网络环境并通过模拟对PUSH-PULL的7种策略进行仿真,模拟仿真结... DTN网络是新一代的网络体系结构,PUSH-PULL是解决DTN网络中拥塞控制的一种有效策略,介绍了DTN网络中拥塞控制的研究现状,并对PUSH-PULL的各个策略进行了阐述,通过NS2搭建DTN网络环境并通过模拟对PUSH-PULL的7种策略进行仿真,模拟仿真结果表明,采用PUSH-PULL策略的可以有效的提高信息的传输率. 展开更多
关键词 DTN push-pull NS2 仿真
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高并发数据共享系统基于Push-Pull混合模式的性能优化
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作者 王子光 王子明 《现代信息科技》 2018年第4期65-67,共3页
随着面向服务架构的大规模分布式系统的应用,多个系统之间由于数据集成的需要,彼此间要高并发传递大量数据,特别是状态数据的同步,对数据的实时性要求越来越高。数据单纯通过客户端以Pull(拉取)模式获取已经不能满足实时性要求,高频率... 随着面向服务架构的大规模分布式系统的应用,多个系统之间由于数据集成的需要,彼此间要高并发传递大量数据,特别是状态数据的同步,对数据的实时性要求越来越高。数据单纯通过客户端以Pull(拉取)模式获取已经不能满足实时性要求,高频率服务调用也会给服务端数据库带来较大的压力。单纯通过服务端以Push(推送)模式推送给客户端也不能满足客户端对个性化数据的需求,大量推送既会给服务端带来较大压力,又会造成客户端数据处理不及时。本文针对高并发数据共享系统应用过程中产生的性能问题,提出一种基于pub/sub消息处理的Push-Pull混合模式优化方案,实践表明该方案极大地提高了系统可用性及数据安全性,对同类系统的性能优化具有较好的借鉴作用。 展开更多
关键词 高并发 数据共享系统 性能优化 pub/sub消息处理 push-pull混合模式
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Push-Pull型不对称钴(III)咔咯的合成与性质 被引量:1
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作者 张振 温俊霞 +2 位作者 张生玉 朱卫华 梁旭 《合成化学》 CAS 北大核心 2020年第9期759-763,共5页
酸催化下,在水-甲醇体系中,合成并分离了3种含有Push-Pull取代基的新型低对称A2B型钴(III)咔咯,其结构经1H NMR,19F NMR和HR-MS(ESI-TOF)表征。并利用光谱学和电化学方法对3种钴咔咯的电子结构进行了深入研究。结果表明:引入Push-Pull... 酸催化下,在水-甲醇体系中,合成并分离了3种含有Push-Pull取代基的新型低对称A2B型钴(III)咔咯,其结构经1H NMR,19F NMR和HR-MS(ESI-TOF)表征。并利用光谱学和电化学方法对3种钴咔咯的电子结构进行了深入研究。结果表明:引入Push-Pull型不对称对钴(III)咔咯分子的电子结构有明显的调控作用,且随着引入基团的改变而产生显著的规律性差异,为进一步的分子设计、合成及其应用奠定基础。 展开更多
关键词 push-pull型咔咯 光谱性质 电化学性质 电子结构 合成
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Exploiting push-pull strategy to combat the tea green leafhopper based on volatiles of Lavandula angustifolia and Flemingia macrophylla 被引量:10
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作者 HAN Shan-jie WANG Meng-xin +3 位作者 WANG Yan-su WANG Yun-gang CUI Lin HAN Bao-yu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第1期193-203,共11页
Thirteen volatile compounds were identified from Flemingia macrophylla plants. Eight major components significantly attracted the tea green leafhoppers, Empoasca flavescens F. Based on their relative abundances, follo... Thirteen volatile compounds were identified from Flemingia macrophylla plants. Eight major components significantly attracted the tea green leafhoppers, Empoasca flavescens F. Based on their relative abundances, following synthetic blends were made for field experiments: 1) eight-component-attractant blend included Z-3-hexen-1-ol, Z-3-hexenyl acetate, Z-ocimene, Me SA, Z-3-hexenyl butyrate, dodecane, hexadecane and nonanal at 10, 10, 1, 11, 2, 6, 2 and 4 mg mL^-1 in n-hexane, respectively;2) four-component-attractant blend #1 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and nonanal at 2, 10, 10 and 4 mg mL^-1 in n-hexane, respectively;3) four-component-attractant blend #2 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and Me SA at 2, 10, 10 and 11 mg mL^-1 in n-hexane, respectively. Thymol and 1-methoxy-4-methyl-2-(1-methylethyl)-benzene, identified from Lavandula angustifolia aeration samples, significantly repelled the leafhopper as strong repellents when tested alone or in combination at 10 mg mL^-1. For field bioassays, each attractant lure was attached to a bud green sticky board hung from a bamboo stick at above tea plant level for catching the leafhoppers, whereas the repellent dispenser was tied to a tea branch inside tea clump for pushing the leafhoppers away from tea clumps. The results showed that the eight-component-attractant blend caught similar numbers of the leafhopper as did the four-component-attractant blend #1 at about 53–79 leafhoppers/trap/day, which were significantly higher than those on the hexane-control bud green sticky boards. Average leafhopper catches from un-baited sticky boards were about 51–73 leafhoppers/trap/day when pushed by the repellents placed inside tea plants, with the two-component-repellent blend being more effective than their single components. When the two-component-repellent blend was further tested with the three attractant blends in a push-pull fashion, average trap catches ranged from 62 to 92 leafhoppers/trap/day. Control efficacy on the leafhoppers within the push-pull zones increased progressively from day 1(43%) to day 5(73%). This push-pull approach might have a great potential as a green control strategy for combating the tea green leafhoppers. 展开更多
关键词 push-pull strategy ATTRACTANTS REPELLENTS Flemingia macrophylla tea green leafhopper tea plantation
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Influential Factors in Employment Location Selection Based on “Push-Pull” Migration Theory—A Case Study in Three Gorges Reservoir Area in China 被引量:10
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作者 XU Ding-de ZHANG Ji-fei +3 位作者 XIE Fang-ting LIU Shao-quan CAO Meng-tian LIU En-lai 《Journal of Mountain Science》 SCIE CSCD 2015年第6期1562-1581,共20页
In China, farmers employed in non-farm work have become important socio-economic actors, but few studies have examined the farmers' perspective in making their work location choices. Based on "push-pull"... In China, farmers employed in non-farm work have become important socio-economic actors, but few studies have examined the farmers' perspective in making their work location choices. Based on "push-pull" migration theory, this paper utilizes sectional data from a 2013 survey of farmers in China's Three Gorges Reservoir area to empirically analyze the factors influencing migrant workers' choice of employment location. The results indicate that 60.46% of laborers have migrated from their home province, whereas 39.54% have remained in their home province. Focusing on personal, household, and community characteristics—in addition to the economic characteristics of the sample counties—multinomial logistic regression models reveal that farmer-laborers' employment location decisions are influenced by their personal capital endowment(age, years of education and social networks), family structure(the number of laborers, elders, children and students), home village characteristics(location, economic development level and the degree of relief of the land) and home county economic development level. Notably, male and female laborers' location decisions reveal a converging trend, and their differences are not pronounced. Per capita arable land area has little influence on location decisions, whereas the educational level of laborers has a significant impact. The results differ significantly from those found in previous studies. 展开更多
关键词 Off-farm employment Location selection Migrants push-pull migration theory Three Gorges Reservoir region China
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Push-Pull Finite-Time Convergence Distributed Optimization Algorithm 被引量:1
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作者 Xiaobiao Chen Kaixin Yan +3 位作者 Yu Gao Xuefeng Xu Kang Yan Jing Wang 《American Journal of Computational Mathematics》 2020年第1期118-146,共29页
With the widespread application of distributed systems, many problems need to be solved urgently. How to design distributed optimization strategies has become a research hotspot. This article focuses on the solution r... With the widespread application of distributed systems, many problems need to be solved urgently. How to design distributed optimization strategies has become a research hotspot. This article focuses on the solution rate of the distributed convex optimization algorithm. Each agent in the network has its own convex cost function. We consider a gradient-based distributed method and use a push-pull gradient algorithm to minimize the total cost function. Inspired by the current multi-agent consensus cooperation protocol for distributed convex optimization algorithm, a distributed convex optimization algorithm with finite time convergence is proposed and studied. In the end, based on a fixed undirected distributed network topology, a fast convergent distributed cooperative learning method based on a linear parameterized neural network is proposed, which is different from the existing distributed convex optimization algorithms that can achieve exponential convergence. The algorithm can achieve finite-time convergence. The convergence of the algorithm can be guaranteed by the Lyapunov method. The corresponding simulation examples also show the effectiveness of the algorithm intuitively. Compared with other algorithms, this algorithm is competitive. 展开更多
关键词 DISTRIBUTED Optimization FINITE Time CONVERGENCE Linear Parameterized NEURAL Network push-pull Algorithm Undirected Graph
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A Case Study of China as an Exporter of Higher Education Using Push-Pull Theory
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作者 Zhang Li 《Review of Global Academics》 2014年第4期277-292,共16页
Developing countries establishing international branch campuses (IBCs) is an emerging trend, and currently compromises 17% percent of the market in terms of the number of IBCs. While researchers have applied differe... Developing countries establishing international branch campuses (IBCs) is an emerging trend, and currently compromises 17% percent of the market in terms of the number of IBCs. While researchers have applied different social science perspectives to analyze cross-border higher education and IBCs, they usually emphasize developed countries as exporters and developing countries as importers of higher education. The phenomenon of developing countries as exporters of higher education has seldom been looked at so far in the research. This paper first describes the current development of IBCs, then illustrates what social science perspectives have been used on this topic, followed by how push-pull theory relates to developing countries being exporters of IBCs. The paper aims to combine E.S. Lee's push-pull theory and Gu's push-pull framework to analyze the push and pull factors in their advertisement and marketing claims when higher education institutions (HEIs) from developing countries plan to establish branch campuses in foreign countries. Soochow University in Laos is used as a case study. Furthermore, intermediate barriers and solutions are also identified in this case study. However, this case study has its own uniqueness and only serves heuristic for future research. 展开更多
关键词 push-pull theory international branch campuses cross-border higher education importers and exporters of higher education
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Comparison of the severity of injury of hippocampal neuron in rats induced by simulated push-pull maneuver at various degrees
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作者 Suhong Guo Hui Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第1期10-13,共4页
BACKGROUND: Push-pull effect is often caused during maneuver, and the changes of unconsciousness induced can affect or damage cerebral neurons at various degrees. OBJECTIVE: To observe the effect of simulated push-p... BACKGROUND: Push-pull effect is often caused during maneuver, and the changes of unconsciousness induced can affect or damage cerebral neurons at various degrees. OBJECTIVE: To observe the effect of simulated push-pull maneuver at various degrees on injury of hippocampal neurons in rats and analyze its phase effect. DESIGN: Randomized control study.SETTING : Physiological Department of Jilin Medical College.MATERIALS: A total of 40 healthy male Wistar rats, of clean grade, weighting 205-300 g, aged 3-4 months, were randomly divided into control group (n=4) and three push-pull experimental groups, including +2 Gz group (intensity: -2 Gz to +2 Gz, n=12), +6 Gz group (-6 Gz to +6 Gz, n=12) and +8 Gz group (-8 Gz to +8 Gz, n=12).METHODS: The experiment was completed in the Physiological Department of Jilin Military Medical College from March 2002 to May 2003. ① Rats in the experimental groups were put at the specially rolling arm of animal centrifugal machine. Then, they were pushed and pulled with ±2 Gz, ±6 Gz and ±8 Gz, respectively. The jolt was 1 Gz/s. However, rats in control group were not treated with any ways. ② Stroke index and neurological evaluation were performed on rats in the experimental groups at 0.5, 6 and 24 hours after push-pull. Stroke index was 25 points in total. The higher the scores were, the severer the cerebral injury was. Neurological evaluation was 10 points in total. The higher the scores were, the severer the nerve injury was. ③ Hippocampal tissue in brain of rats were selected to cut into sections at each time points, and form and distribution of neurons were observed in hippocampal areas with HE staining. Degrees of neuronal injury in hippocampal CA1 area were assayed after push-pull at various degrees with electron microscope. ④ Measurement data were compared with t test.MAIN OUTCOME MEASURES:① Stroke index and neurological evaluation; ② form and distribution of neurons in hippocampal areas;③ degrees of neuronal injury in hippocampal CA1 area.RESULTS: A total of 40 rats were involved in the final analysis. ① Stroke index and neurological evaluation of rats in experimental groups: At 30 minutes and 6 hours after push-pull exposure, stroke index and neurological evaluation were higher in ±6Gz group and ±8 Gz group than those in control group (P 〈 0.01), especially at 6 hours after push-pull exposure, those in ±8 Gz group were the highest at each time points [(11.00±2.16), (5.75±1.70) points]. At 24 hours after exposure, those were decreased as compared with those within the former two time points, but the values were still higher than those in control group (P 〈 0.05-0.01). ② Results of HE staining: At 6 and 24 hours after exposure, partially neuronal degeneration was observed in pyramidal layer in ±6 Gz group and ±8 Gz group, including crenation of neurons, tdangle or polygon, and karyopycnosis, especially the injury in ±8 Gz group was the most obvious at 6 hours after exposure. ③ Results of ultrastructure with electron microscope: Partially neuronal degeneration at various degrees was observed in hippocampal CA1 area in ±2 Gz group at 6 hours after exposure and in ±6 Gz group and ±8 Gz group at 6 and 24 hours after exposure. At 6 hours after exposure, nucleus of hippocampal neurons in ±8 Gz group was irregular and umbilication. Caryotin was aggregated, nuclear matrix was swelled and disorder, and vacuolation was also observed. Rough endoplasmic reticulum was expanded, mitochondrium was swelled, and crista was disappeared.CONCLUSION: ① Push-pull cannot damage hippocampal neurons of rats in ±2 Gz group. ② Exposure can cause injury of hippocampal neurons of rats in ±6Gz group and ±8 Gz group, especially the injury is the severest at 6 hours after exposure in ±8 Gz group and relieves gradually 24 hours later. 展开更多
关键词 Comparison of the severity of injury of hippocampal neuron in rats induced by simulated push-pull maneuver at various degrees
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Basic Study on Perfect Push-Pull Local Ventilation
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作者 Yukihiro Hattori Koichi Nakabayashi +1 位作者 Osami Kitoh Motoyuki Ito 《Journal of Energy and Power Engineering》 2012年第12期1940-1944,共5页
In this study, the authors experimentally investigated the changes of the mean velocity component profiles, half-widths (b12), turbulence intensities, Reynolds shear stress and intermittency of turbulence of a trans... In this study, the authors experimentally investigated the changes of the mean velocity component profiles, half-widths (b12), turbulence intensities, Reynolds shear stress and intermittency of turbulence of a transient plane turbulent jet developing from a jet exit into a hood opening. The values of maximum mean-velocity and half-widths of the axial velocity profile along the center-line of the jet are greater than those for a fully developed two-dimensional jet. Turbulence intensity in the axial direction is not affected by the flow rate ratio. At the same time, turbulence intensity in the lateral direction becomes greater as the hood is approached and the flow rate ratio Q3/Q1 becomes larger (QI is jet flow rate from nozzle and Q3 is suction flow rate produced by the hood). These experimental results are in accord with the distributions of production terms in the axial and lateral directions. Reynolds shear stress becomes smaller as the flow rate ratio becomes larger near the hood. Dimensionless distance y1/br2, from the center axis of the flow to the point where intermittency factor y becomes a constant value, narrows as the flow rate ratio becomes larger near the hood. 展开更多
关键词 Turbulence intensity intermittency factor Reynolds shear stress push-pull ventilation.
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小区播种机供种装置的技术研究现状及展望
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作者 叶岩 吴泽全 +5 位作者 蔡晓华 刘俊杰 杨存志 李源源 赵丽平 李尧 《农机化研究》 北大核心 2025年第2期262-268,共7页
供种装置是小区播种机的关键装置之一。为此,简述了国内外小区播种机配套的主要供种装置与技术研究现状。从供种装置的主要结构形式和供种方式出发,阐述了现有单漏斗式、中央锥斗式、弹匣式和新型弹匣式供种装置的工作原理、结构组成和... 供种装置是小区播种机的关键装置之一。为此,简述了国内外小区播种机配套的主要供种装置与技术研究现状。从供种装置的主要结构形式和供种方式出发,阐述了现有单漏斗式、中央锥斗式、弹匣式和新型弹匣式供种装置的工作原理、结构组成和前沿技术研究进展。同时,分析了当前国内外供种装置的研发现状,列举国内外具有代表性的小区播种机供种装置的研究成果,并结合国内供种装置发展现状展望了国内小区播种机配套供种装置的未来发展方向,为企业及研究人员了解国内小区播种机供种装置的技术研究现状提供一定的参考。 展开更多
关键词 小区播种机 供种装置 研究现状
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Comparative Analysis of Five Different Homologous Feeder Cell Lines in the Ability to Support Rhesus Embryonic Stem Cells
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作者 陈栋梁 李荣荣 +5 位作者 张敬 卢斌 魏强 王淑芬 谢云华 季维智 《Zoological Research》 CAS CSCD 北大核心 2009年第4期345-353,共9页
In our previous study, five homologous feeder cell lines, Monkey ear skin fibroblasts (MESFs), clonally derived fibroblasts from the MESFs (CMESFs), monkey oviductal fibroblasts (MOFs), monkey follicular granulo... In our previous study, five homologous feeder cell lines, Monkey ear skin fibroblasts (MESFs), clonally derived fibroblasts from the MESFs (CMESFs), monkey oviductal fibroblasts (MOFs), monkey follicular granulosa fibroblast-like (MFGs) cells, monkey follicular granulosa epithelium-like (MFGEs) cells, were developed for the maintenance of rhesus embryonic stem cells (rESCs). We found that MESFs, CMESFs, MOFs and MFGs, but not MFGEs, support the growth of rhesus embryonic stem cells. Moreover, we detected some genes that are upregulated in supportive feeder cell lines by semi-quantitative PCR. In the present study, we applied the GeneChip Rhesus Macaque Genome Array of Affymetrix Corporation to study the expression profiles of these five feeder cell lines, in purpose to find out which cytokines and signaling pathways were important in maintaining the rESCs, mRNAs of eight genes, including GREM2, bFGF, KITLG, DKK3, GREM1, AREG, SERPINF1 and LTBP1, were found to be upregulated in supportive feeder cell lines, but not in MFGE. The results indicate that many signaling pathways may play redundant roles in supporting the undifferentiated growth and maintenance of pluripotency in rESCs. 展开更多
关键词 Embryonic stem cells Rhesus monkey feeders SELF-RENEWAL PLURIPOTENCY Expression profile Signaling pathway
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Mechanical Analysis and Optimization of ITER Upper ELM Coil & Feeder 被引量:1
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作者 张善文 宋云涛 +8 位作者 王忠伟 卢速 戢翔 杜双松 刘旭峰 冯昌乐 杨洪 王松可 罗志仁 《Plasma Science and Technology》 SCIE EI CAS CSCD 2014年第8期794-799,共6页
International thermonuclear experimental reactor (ITER) edge localized mode (ELM) coils are used to mitigate or suppress ELMs. The location of the coils in the vacuum vessel and behind the blankets exposes them to... International thermonuclear experimental reactor (ITER) edge localized mode (ELM) coils are used to mitigate or suppress ELMs. The location of the coils in the vacuum vessel and behind the blankets exposes them to high radiation levels and high temperatures. The feeders provide the power and cooling water for ELM coils. They are located in the chinmey ports and experience lower radiation and temperature levels. These coils and feeders work in a high magnetic field environment and are subjected to alternating electromagnetic force due to the interaction between high magnetic field and alternating current (AC) current in the coils. They are also subjected to thermal stresses due to thermal expansion. Using the ITER upper ELM coil and feeder as an example, mechanical analyses are performed to verify and optimize the updated design to enhance their structural performance. The results show that the conductor, jacket and bracket can meet the static, fatigue and crack threshold criteria. The optimization indicates that adding chamfers to the bracket can reduce the high stress of the bracket, and removing two rails can reduce the peak reaction force on the two rails arising from thermal expansion. 展开更多
关键词 ELM coil feeder mechanical analysis OPTIMIZATION
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Effects of feeder layer and BRL conditioned medium on mouse embryonic stem cells 被引量:9
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作者 TsungHsiaochien christine,L.Mummery 《Cell Research》 SCIE CAS CSCD 1990年第1期35-52,共18页
In vitro growth and maintenance of embryonic stem (ES) cell lines derived from ICM cells of various blastocysts of 129 strain mice, the sustenance of their pluripotency and normal karyotype depend on the feeder layer ... In vitro growth and maintenance of embryonic stem (ES) cell lines derived from ICM cells of various blastocysts of 129 strain mice, the sustenance of their pluripotency and normal karyotype depend on the feeder layer of mouse embryonic fibroblasts (MEF). Compared with the feeder layer of MEF cells, medium conditioned by Buffalo rat liver cells (BRL-CM) is able to maintain pluripotency and karyo-typic normality of ES cells only in short term cell propagation. Besides, ES cells grown in BRL-CM are also capable of aggregation with 8-cell embryos of Swiss strain and develop into germ line chimaeras. Modification to the method of aggregating ES cells with early embryos by making a hole in agar layer on the top of MEF feeder cells was shown to be more convenient and efficient than the conventional microdrop method. 展开更多
关键词 embryonic stem (ES) cells feeder layer BRL conditioned medium chimearas.
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Relationship between alumina mixing characteristics and feeder configurations in aluminum reduction cells 被引量:3
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作者 Hong-liang ZHANG Shuai YANG Jie LI 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2017年第11期2512-2520,共9页
Because of the increasingly prominent problem of alumina content inhomogeneity in large or super-scale aluminum reduction cells,a transient numerical model for the alumina mixing process was developed for a400kA cell,... Because of the increasingly prominent problem of alumina content inhomogeneity in large or super-scale aluminum reduction cells,a transient numerical model for the alumina mixing process was developed for a400kA cell,and the relationship between the alumina content distribution and electrolyte flow field was analyzed.In the ANSYS software platform,several numerical simulation cases were presented to display the influence of the feeder configuration on the alumina mixing characteristics.The results showed that a large vortex flow of the molten electrolyte is beneficial for alumina mixing and uniform distribution in the inner areas of the vortexes.The alumina particles reach the inter-electrode zone in10?15s from the beginning of the feeding action,and the risk of early precipitation occurs in10?25s after the feeding.It was also found that a suitable grouping of feeders could reduce the content fluctuation and gradient.Therefore,a feeding on demand strategy was proposed,and the simulation results showed that although the spatial characteristics are not changed,the uniformity of the alumina content was markedly improved. 展开更多
关键词 aluminum electrolysis content distribution feeder configuration alumina precipitation
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Probabilistic Fuzzy Approach to Assess RDS Vulnerability and Plan Corrective Action Using Feeder Reconfiguration 被引量:2
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作者 Mini S Thomas Rakesh Ranjan Roma Raina 《Energy and Power Engineering》 2012年第5期330-338,共9页
Two common problems for a typical Power distribution system are voltage collapse & instability. Challenge is to identify the vulnerable nodes and apply the effective corrective actions. This paper presents a proba... Two common problems for a typical Power distribution system are voltage collapse & instability. Challenge is to identify the vulnerable nodes and apply the effective corrective actions. This paper presents a probabilistic fuzzy approach to assess the node status and proposes feeder reconfiguration as a method to address the same. Feeder reconfiguration is altering the topological structures of distribution feeders by changing the open/closed states of the sectionalizing and ties switches. The solution is converge using a probabilistic fuzzy modeled solution, which defines the nodal vulnerability index (VI) as a function of node voltage and node voltage stability index and predicts nodes critical to voltage collapse. The information is further used to plan best combination of feeders from each loop in distribution system to be switched out such that the resulting configuration gives the optimal performance i.e. best voltage profile and minimal kW losses. The proposed method is tested on established radial distribution system and results are presented. 展开更多
关键词 Branch VOLTAGE Three Phase Load Flow VOLTAGE Stability INDEX (SI) Radial Distribution System (RDS) Monte Carlo Probability Distributions Fuzzy Set Node VULNERABILITY Index(VI) feeder Reconfiguration
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Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells 被引量:3
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作者 Hui-Xian Wang Xiao-Wei Gao +4 位作者 Bing Ren Yan Cai Wen-Jing Li Yu-Li Yang Yi-Jian Li 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2017年第7期1021-1027,共7页
AIM: To explore the possibility of human umbilical cord mesenchymal stem cells(h UCMSCs), human umbilical vein endothelial cells(h UVECs), human dental pulp stem cells(h DPSCs) and human periodontal ligament st... AIM: To explore the possibility of human umbilical cord mesenchymal stem cells(h UCMSCs), human umbilical vein endothelial cells(h UVECs), human dental pulp stem cells(h DPSCs) and human periodontal ligament stem cells(h PDLSCs) serving as feeder cells in co-culture systems for the cultivation of limbal stem cells.METHODS: Different feeder layers were cultured in Dulbecco's modified Eagle's medium(DMEM)/F12 and were treated with mitomycin C. Rabbits limbal stem cells(LSCs) were co-cultured on h UCMSCs, h UVECs, h DPSCs, h PDLSCs and NIH-3T3, and then comparative analysis were made between each group to see their respective colony-forming efficiency(CFE) assay and immunofluorescence(IPO13,CK3/12).RESULTS: The efficiency of the four type cells in supporting the LSCs morphology and its cellular differentiation was similar to that of NIH-3T3 fibroblasts as demonstrated by the immunostaining properties analysis, with each group exhibiting a similar strong expression pattern of IPO13, but lacking CK3 and CK12 expression in terms of immunostaining. But h UCMSCs, h DPSCs and h PDLSCs feeder layers were superior in promoting colony formation potential of cells when compared to h UVECs and feedercell-free culture.CONCLUSION: hUCMSCs, hDPSCs and hPDLSCs can be a suitable alternative to conventional mouse NIH-3T3 feeder cells, so that risk of zoonotic infection can be diminished. 展开更多
关键词 limbal stem cells feeder layers umbilical cord mesenchymal stem cells umbilical vein endothelial cells dental pulp stem cells periodontal ligament stem cells
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Dynamic Analysis for the Global Performance of An SPM-Feeder-Cage System Under Waves and Currents 被引量:2
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作者 Cristian CIFUENTES M.H.KIM 《China Ocean Engineering》 SCIE EI CSCD 2015年第3期415-430,共16页
In the present study, the dynamic response of a coupled SPM-feeder-cage system under irregular waves and shear currents is analyzed. A numerical model is developed by using the commercial software Orca Flex. Hydrodyna... In the present study, the dynamic response of a coupled SPM-feeder-cage system under irregular waves and shear currents is analyzed. A numerical model is developed by using the commercial software Orca Flex. Hydrodynamics coefficients of the vessel are calculated by using a 3D diffraction/radiation panel program. First- and second-order wave forces are included in the calculations. Morison equation is used to compute the drag force on line elements representing the net. Drag coefficients are determined at every time step in the simulation considering the relative normal velocity between the structural elements and the fluid flow. The dynamic response of the coupled system is analyzed for various environments and net materials. The results of the study show the effects of solidity ratio of the net and vertical positions of the cage on the overall dynamic response of the system, confirming the viability of this type of configuration for future development of offshore aquaculture in deep waters. 展开更多
关键词 offshore aquaculture SPM(single point mooring) feeder vessel connecting lines operational/survival environment drag force equivalent net cage grouping net modeling line tensions solidity ratio
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