Development and evaluation of a RT-RAA-combined CRISPR/Cas12a assay for the detection of African horse sickness virus

African horse sickness(AHS)is an acute and fatal vectorborne infectious disease of equids,caused by the African horse sickness virus(AHSV).The World Organization for Animal Health(WOAH)has classified AHS as a notifiable animal disease,and AHS has also been classified as a Class I animal infectious disease in China.AHS is mainly found in Africa,the Middle East and the Arabian Peninsula.China is currently recognized by the WOAH as an AHS-free zone.

High Altitude Adaptation and Phylogenetic Analysis of Tibetan Horse Based on the Mitochondrial Genome

To investigate genetic mechanisms of high altitude adaptations of animals living in the Tibetan Plateau, three mitochondrial genomes （mt-genome） of Tibetan horses living in Naqu （4,500 m） of Tibetan, Zhongdian （3,300 m） and Deqin （3,100 m） of Yunnan province were sequenced. The structures and lengths of these three mt-genomes are similar to the Cheju horse, which is related to Tibetan horses, but little shorter than the Swedish horse. The pair-wise identity of these three horses on nucleotide level is more than 99.3%. When the gene encoding the mitochondrial protein of Tibetan horses was analyzed, we found that NADH6 has higher non-synonymous mutation rate in all of three Tibetan horses. This implies that NADH6 may play a role in Tibetan horses＇ high altitude adaptation. NADH6 is one of the subunits of the complex I in the respiratory chain. Furthermore, 7 D-loop sequences of Tibetan horse from different areas were sequenced, and the phylogeny tree was constructed to study the origin and evolutionary history of Tibetan horses. The result showed that the genetic diverse was high among Tibetan horses. All of Tibetan horses from Naqu were clustered into one clade, and Tibetan horses from Zhongdian and Deqin were clustered into others clades. The first molecular evidence of Tibetan horses indicated in this study is that Tibetan horse population might have multiple origins.

Father,Son and A Horse

Once there lived a very old peasant in a village. His name was Jack.He had a horse.It was almost as old as him. One day he set off with his young son to sell the horse before it died.They walked because the father did not want the horse to be too tired. They met two neighbours (邻居)on their way.The neighbours said, "Hello,why are

如何理解“closing the barn door after the horse have escaped”?

然而,由教育部考试中心组织编写、高等教育出版社出版的《全国硕士研究生入学英语考试分析(2001年版)》对这道题的分析是:“……并且打个比喻说,马逃走了,关上马棚的大门,人们却发现骑在马上的正是这些受过教育的人他们自己。”显然,这样的分析对理解原文并不能起多少作用,读后考生仍然感到云里雾里。

Atmospheric Profile Retrieval with AIRS Data and Validation at the ARM CART Site

The physical retrieval algorithm of atmospheric temperature and moisture distribution from the Atmospheric InfraRed Sounder （AIRS） radiances is presented. The retrieval algorithm is applied to AIRS clear-sky radiance measurements. The algorithm employs a statistical retrieval followed by a subsequent nonlinear physical retrieval. The regression coefficients for the statistical retrieval are derived from a dataset of global radiosonde observations （RAOBs） comprising atmospheric temperature, moisture, and ozone profiles. Evaluation of the retrieved profiles is performed by a comparison with RAOBs from the Atmospheric Radiation Measurement （ARM） Program Cloud And Radiation Testbed （CART） in Oklahoma, U. S. A.. Comparisons show that the physically-based AIRS retrievals agree with the RAOBs from the ARM CART site with a Root Mean Square Error （RMSE） of 1K on average for temperature profiles above 850 hPa, and approximately 10% on average for relative humidity profiles. With its improved spectral resolution, AIRS depicts more detailed structure than the current Geostationary Operational Environmental Satellite （GOES） sounder when comparing AIRS sounding retrievals with the operational GOES sounding products.

The Emergency Department Crash Cart: A systematic review and suggested contents

BACKGROUND: As the field of Emergency Medicine grows worldwide, the importance of an Emergency Department Crash Cart(EDCC) has long been recognized. Yet, there is paucity of relevant peer-reviewed literature specifically discussing EDCCs or proposing detailed features for an EDCC suitable for both adult and pediatric patients. METHODS: The authors performed a systematic review of EDCC-specific literature indexed in Pubmed and Embase on December 20, 2016. In addition, the authors reviewed the 2015 American Heart Association(AHA) guidelines for cardiopulmonary resuscitation and emergency cardiovascular care, the 2015 European Resuscitation Council(ERC) guidelines for resuscitation, and the 2013 American College of Surgeons(ACS) Advanced Trauma Life Support(ATLS) 9 th edition.RESULTS: There were a total of 277 results, with 192 unique results and 85 duplicates. After careful review by two independent reviewers, all but four references were excluded. None of the four included articles described comprehensive contents of equipment and medications for both the adult and pediatric populations. This article describes in detail the final four articles specific to EDCC, and proposes a set of suggested contents for the EDCC. CONCLUSION: Our systematic review shows the striking paucity of such a high impact indispensable item in the ED. We hope that our EDCC content suggestions help enhance the level of response of EDs in the resuscitation of adult and pediatric populations, and encourage the implementation of and adherence to the latest evidence-based resuscitation guidelines.

Mathematical modelling of translational motion of rail-guided cart with suspended payload

In this paper modelling of the translational motion of transportation rail-guided cart with rope suspended payload is considered. The linearly moving cart,driven by a travel mechanism,is modelled as a discrete six degrees of freedom (DOF) dynamic system. The hoisting mechanism for lowering and lifting the payload is considered and is included in the dynamic model as one DOF system. Differential equations of motion of the cart elements are derived using Lagrangian dynamics and are solved for a set of real-life constant parameters of the cart. A two-sided interaction was observed between the swinging payload and the travel mechanism. Results for kinematical and force parameters of the system are obtained. A verification of the proposed model was conducted.

Effects of Motivation and Demography on the Quantitative Plausibility of the Trojan Horse Defence Against Possession of Child Pornography

The Enhanced Complexity Model( ECM) developed previously has been further extended to produce a Motivationally Enhanced Complexity Model( MECM) which enables the degree of motivation,capability and opportunity of a hypothetical Trojan Horse author to be included in quantifying the relative plausibility of competing explanations for the existence of uncontested digital evidence.This new model has been applied to the case of the Trojan Horse defence( THD) against the possession of child pornography.Our results demonstrate that the THD in this case cannot be plausibly sustained unless it can be shown that an ‘off-theshelf'( OTS) Trojan Horse for this task is available and it is not detectable by the target computer,at the material time.

A Therapeutic Sheep in Metastatic Wolf’s Clothing: Trojan Horse Approach for Cancer Brain Metastases Treatment

Early-stage brain metastasis of breast cancer(BMBC), due to the existence of an intact blood–brain barrier(BBB), is one of the deadliest neurologic complications. To improve the efficacy of chemotherapy for BMBC, a Trojan horse strategy-based nanocarrier has been developed by integrating the cell membrane of a brain-homing cancer cell and a polymeric drug depot. With the camouflage of a MDA-MB-231/Br cell membrane, doxorubicin-loaded poly(D, L-lactic-co-glycolic acid) nanoparticle(DOX-PLGA@CM) shows enhanced cellular uptake and boosted killing potency for MDA-MB-231/Br cells. Furthermore, DOX-PLGA@CM is equipped with naturally selected molecules for BBB penetration, as evidenced by its boosted capacity in entering the brain of both healthy and early-stage BMBC mouse models. Consequently, DOX-PLGA@CM effectively reaches the metastatic tumor lesions in the brain, slows down cancer progression, reduces tumor burden, and extends the survival time for the BMBC animal.Furthermore, the simplicity and easy scale-up of the design opens a new window for the treatment of BMBC and other brain metastatic cancers.

Functional Microvascular Anatomy of the Horse Eye: A Scanning Electron Microscopic Study of Corrosion Casts

Objective: This study presents the microvasculature of the horse iris, ciliary process, retina, and choroid and discusses the functional significance of the vasculature. Procedure: Seven horses were used for this study. The ocular vascular system was injected with methylmethacrylate resin via the carotid artery, and the vascular corrosion casts were observed using a scanning electron microscope. Results: The iridial vessels showed a wavy course. The ciliary process was supplied by 2 arterial routes: the iridial and ciliary arterial circles. The subjects displayed a paurangiotic retina with retinal vessels extending only a short distance around the disc. The retinal arterioles and venules ran in closely related pairs, and the capillaries formed hairpin loops. No central retinal artery was seen in the equine eyes examined. The choriocapillaris in the avascular retina was arranged in honeycomb hexagon lobules and formed a more densely packed network than that in the vascular retina. There were 2 distinct venous drainage systems in the horse choroid: the vortex veins and the posterior ciliary veins. The vortex vein ampulla was flattened and showed a slit-like lumen at the merge site with the ophthalmic vein. The vortex veins demonstrated a marked constriction before leaving the eye. Discussion: The 2 choroidal drainage systems may compensate each other in event of occlusion. The ampulla and the constriction in the vortex veins may act as a valve regulating the blood flow to keep the eye at an optimum size and the intraocular pressure within the normal physiological range.

Economic Impact of the Horse Industry： A Special Reference to Spain

The economy of the equestrian sector encompasses all the activities related to the equine world. All activities revolving around the use of a horse as entertainment, sport or business play a role in the ＂horse industry＂. These activities take place due to the existence of people demanding horses and a variety of goods and services associated with them Previous studies reveal that as income per capita becomes larger, the equestrian demand increases and, consequently, the global expenditure incurred by those individuals interested in horses. More than 500,000 horse, 9,000 breeders and 50,000 equestrian businesses, show the economic importance of the equestrian sector in Spain. That part of global expenditure that adds to the GDP of a country is what is generally defined as economic impact of the sector. Its relation will depend mostly on： the percentage of the expenditure that adds to national production and the participation in the country＇s global expenditure of those domestic goods and services associated with the equestrian demand. The activities related to sports are those with most economic impact, about 8,000 ∈ per horse, measured as total expenditure or with respect to the employment that they generate in the economy.

“horse＂趣闻The stories of HORSE

在中国的十二生肖中，马（horse）被当作一种神圣的，传奇式的动物。它四肢强壮，叫声响亮，善跑，健美，并能认路。在中国神话故事《西道纪》中，唐僧乘坐的是由小白龙变成的白龙马；在《圣经》（Bible）中，耶稣和他的天国军士也用白马：在希腊神话中，太阳神Apollo也用白马作为交通工具等。由此看来，马不论在东方，还是西方；也无论在童话，还是神话里，都被当作一种善解人意，能给主人以信赖的吉祥动物。

A report of left dorsal displacement of the large colon in a tropical horse

We report one such case which was diagnosed intraoperatively as left dorsal colon volvulus due to multiple mesenteric abnormalities.A 17-year-old castrated male horse was taken to the Tehran University Veterinary Hospital for treatment of metacarpal wound accompanied by severe abdominal distension and acute colic.The treatment and measurement were taken for a month,and the prepared biopsy indicated that the healing trend was obvious.Unfortunately,prior to discharge,the clinical colic manifestations emerged and the animal suddenly died.Dilated large intestine was palpated per rectum and a ventral midline exploratory laparotomy was performed,a complete volvulus of the ascending colon was identified with multiple mesenteric anomalies of unknown aetiology.The pathologic changes observed in this study accurately reflect those changes reported in horse with naturally occurring colonic volvulus and can serve as a reference for subsequent studies on attenuating bowel injury.The present study results can be used to make a scientific assessment of prognosis in the pre-operative,operative,and post-operative management of horses with large colon volvulus.

China-India Relationship and Buddhism—From the perspective of the discursive practice of the White Horse Temple

India and China, even though close in distance, but distant in relationship. Recently, the world has witnessed the improvement of the bilateral relations between the two big nations. The White Horse Temple, as a site constructed by discourses has transformed from‘the first government-funded temple in China'to one of the symbols of China-India friendship in the cultural exchange between the two nations. Critical discourse analysis(CDA) is an interdisciplinary approach to the study of discourse that views language as a form of social practice and focuses on the ways social and political domination are reproduced in text and talk. This paper, starts from the perspective of critical discourse analysis, is exploratory in that it aims to see how the discursive practice of the White Horse Temple constitutes the discourse of China-India relationship and is constituted by the power relationship between the two nations.

The Little Gypsy Horse

1. It was the day after the circus(马戏团) came to our village that Yakub and I first saw the little horse. He looked terrible. Every rib (肋骨)showed clearly. His hair-was coming out in patches. His head hang down.

Plasma and Red Blood Cells Concentration Profiles of Ktamine after Single Intravenous Administration in an Anaesthetic Protocol in Horses

The aim of this study was to describe the concentration profile of ketamine in plasma and red blood cells following an intravenous (IV) bolus in the horse. Ten healthy standardbred horses (two males and height females) 7.7 ± 4.6 (mean value ± SD) years old and weighting 380 ± 21 kg (mean value ± SD) were recruited. The horses were premedicated with acepromazine (0.04 mg·kg-1·IV). Fifteen minutes later they received romifidine (0.08 mg·kg-1·IV), and 5 minutes after they were administered midazolam (0.06 mg·kg-1·IV). Immediately, anaesthesia was induced by ketamine (2.2 mg·kg-1·IV). Venous blood samples were collected at scheduled time points. Plasma and red blood cells (RBCs) concentration of ketamine was assayed using a high performance liquid chromatographic method (HPLC/UV-DAD). The high mean recovery rates, the high sensitivity, the good linearity, suggest a clinical applicability of the analytical method. A bicompartmental model resulted as the most appropriate to describe the ketamine concentration—time profile for both plasma and RBCs. The fitted regression line between ketamine plasma concentrations and RBC concentrations supports the good correlation between ketamine concentrations in plasma and in RBCs. The kinetic parameters of ketamine calculated for RBC are equal or very similar to the plasma ones. The study confirms the kinetic behaviour of ketamine used in the horse as anaesthetic inducers in routine surgery. Finally, the bicompartmental model well describes the ketamine profile also in RBCs, that it is very close to the plasma profile, underlining the great importance of RBCs as blood subcompartment.

Acoustic Myography: Its Assessment of Ground Reaction Forces as Measured through Forelimb and Hind Limb of the Horse during Mild Exercise

Background: Fractures in the limbs of racehorses are common, resulting among other factors, as the result of repeated ground reaction forces on bones and joints, leading to catastrophic failure. Aim: To quantify ground impact transmission through the limb bones of un-shod healthy horses using the non-invasive technique of acoustic myography (AMG). Methods: Four sites were selected for AMG measurements at the walk and trot, hoof wall (site 1) and sites 2 - 4, metacarpal 3, carpals and the radius of the left forelimb of two healthy horses. Measurements were on both rubber and concrete. AMG of the equine hind limb suspensory system was made and analyzed (amplitude and timing) for the proximal suspensory ligament (PSL) and the SDFT/DDFT. Results: AMG signal amplitude at site 1 (1.5 ± 0.2 versus 1.1 ± 1.5) was not found to be significantly different at the trot compared to the walk;however, sites 2, 3 and 4 were all significantly different when compared between the two gaits;site 2 P = 0.008;site 3 P = 0.006;site 4 P = 0.005. AMG signals recorded on the rubber surface had smaller amplitude than the equivalent signal and site on the concrete surface. Ground Reaction Force (GRF) transmission in the equine forelimb was 22 m/sec, whilst that of the hind limb suspensory system was 25 m/sec. Conclusion: Findings indicate that GRFs are transmitted proximally along the limb at considerable speeds, that they are dampened by tissues and structures in the limb, and that the GRFs are present and detectable proximal to the fetlock joint.

Molecular investigation of the Arabian horse breeds of racing, productivity and longevity

The Arabian;horse Equus caballus is belonging to family Equidae that all of its species are in danger of extinction. We sequenced 1059 nucleotides from three mitochondrial DNA protein coding genes for some breeds of the Arabian and English horses designated for race, productivity and longevity in order to investigate their genetic variation. The sequenced genes were NADH dehydrogenase subunit 2 (ND2), cyto-chrome C oxidase subunit 1 (CO1) and ATPase subunit 6 (ATP6). ATP6 discriminated among the different breeds where seven substitutions were recorded of which 2 were non-synonymous. The other loci recoded 8 synonymous substitutions of transitions most of which were among the breeds of longevity. The data of CO1 gene were used to construct the relationship of the Arabian horse to the thoroughbreds. Maximum-likelihood (ML) and Neighbor-joining (NJ) analytical methods were used. The two analytical methods showed clustering of the Arabian breeds in one group and the non-sister relationship within the thoroughbreds. One of the thoroughbreds clustered within the Arabian breeds suggesting the possibility of their out breeding. Increasing tendency in the variation of the studied genes was proportional to the utility of these genes in energy production within the mitochondria. The present study is therefore a preliminary step in the conservational efforts of the Arabian horse of equestrian..

High Plasma Concentrations of Sclerostin, an Inhibitor of the Wnt Signaling Pathway, in Young Horses Affected by Osteochondrosis

Osteochondrosis (OC) is a developmental disease in horses with a significant impact on the horse’s welfare and performance. The early disturbance of enchondral ossification progresses to inflammatory and healing process in older horses. Metabolic pathway analysis showed an obvious dysregulation of several signaling pathways related to cartilage formation and cartilage repair such as Wnt/β-catenin, Indian hedgehog and TGF-β signaling pathways. Other regulated genes appeared to be involved in high carbohydrate diet, abnormal insulin metabolism or inflammation. Sclerostin is an osteocyte-secreted soluble antagonist of the Wnt/β-catenin signaling pathway. It is crucial for osteoblast development and activity and is increased in naturally occurring lesions of equine osteochondrosis. The aim of this study is to compare the circulating sclerostin levels between OC-affected (n = 20) and healthy horses (n = 19). A significant linear regression between plasma sclerostin and age is observed especially in the healthy young horses. The mean plasma sclerostin concentration is significantly higher in young horses suffering from osteochondrosis compared to the control horses. These results reinforce the possible role of the Wnt/β-catenin signaling pathway in the OC pathogeny. The inhibition of this essential pathway could disturb the osteo-chondral differentiation. More studies are currently needed to define the eventual clinic interest of plasma sclerostin as future biomarker in bone and cartilage diseases.

Early pregnancy in the horse revisited–does exception prove the rule?

Early equine pregnancy shares many features with that of more intensively assessed domestic animals species, but there are also characteristic differences. Some of those are poorly understood. Descent of the equine conceptus into the uterine lumen occurs at day 5 to 6 after ovulation but is only possible when the embryo secretes prostaglandin E2. Although maintenance of equine pregnancy probably involves secretion of a conceptus derived anti-luteolytic factor, this agent has not been identified. Rapid growth, conceptus mobility and presence of an acellular capsule at the time of maternal recognition of pregnancy, i.e. between days 12 and 14, are prerequisites to avoid pregnancy loss. Progesterone together with 5α-pregnanes is secreted by the corpus luteum and induces the production of endometrial histotroph which is responsible for conceptus nutrition until placention. A stable contact between the outer trophoblast layer of the allantochorion and the luminal epithelium of the endometrium is not established before days 40 to 42 of pregnancy.