ADAM9 decreases in castration resistant prostate cancer and is a prognostic factor for overall survival

Background A disintegrin and metalloprotease 9 （ADAM9） is a membrane-anchored enzyme which is considered to be involved in some diseases including tumor. However, the role of ADAM9 in castration resistant prostate cancer （CRPC） is not clear. This study aimed to explore the different expressions on protein and messenger RNA （mRNA） level of ADAM9 between hormonal sensitive prostate cancer （HSPC） and CRPC tissue, and find the correlation with prognosis.

猪德尔塔冠状病毒荧光定量PCR检测方法的建立及应用

猪德尔塔冠状病毒(PDCoV)作为一种新型的猪肠道腹泻病毒,建立快速有效的检测手段十分必要。本研究根据PDCoV N基因中的保守序列设计了1对特异性引物,PCR扩增后克隆至pEASY-Blunt Zero载体,构建了阳性质粒pEASY-PDCoV/N。以阳性质粒pEASY-PDCoV/N为模板,建立了检测PDCoV的SYBR GreenⅠ荧光定量PCR方法,并对其敏感性、特异性和重复性等进行了优化与验证。结果显示,C_(1)值与标准模板在5.83×10^(7)~5.83×10^(1)copies/μL范围内呈良好的线性关系,相关系数(R^(2))为0.999,线性方程的斜率为-3.323,最低检测限度为5.83×10^(1)copies/μL。该方法特异性和重复性均较好,对PEDV、PRV、TGEV等病毒均未出现检测信号,批内和批间变异系数分别小于1%和2%。利用建立的PDCoV荧光定量PCR检测方法对60份猪腹泻样品进行核酸检测,阳性率为18.3%,比普通PCR检出率高6.7%。结果表明,该方法可适用于临床快速检测PDCoV,为该病的预防和控制提供技术支持。