Objective To identify the coumarins constituents in Cnidium monnieri and classify ten samples into three groups and this helpful chemical information could be used for the further pharmacological and clinical study on...Objective To identify the coumarins constituents in Cnidium monnieri and classify ten samples into three groups and this helpful chemical information could be used for the further pharmacological and clinical study on C. monnieri. Methods Qualitative analysis of coumarins in C. monnieri was detected by UHPLC-ESI-Q-TOF/MS. Quadrupole TOF/MS in either full scan mode or extracted ion mode was used for the qualitative analysis of the constituents. Relative peak area of each component was used for the hierarchical cluster analysis. Results According to UHPLC-ESI-QTOF-MS data, chemical structures of 28 coumarins in the fruits of C. monnieri were identified, including 19 simple coumarins, seven linear coumarins, and two angular coumarins. Among these constituents, ten coumarins were firstly identified in C. monnieri. In addition, hierarchical cluster analysis suggested that C. monnieri from different regions could be classified into four groups, and this clustering was correlated to the distribution significantly, Xuzhou could be regarded as the genuine producing area. Conclusion UHPLC-ESI-Q-TOF-MS is a viable method for qualitative analysis and quality evaluation of coumarins from the fruit of C. monnieri. Coumarins in C. monnieri exists intra-species variance, which indicates significant meaning for the quality control and choice of famous region drug for C. monnieri in the clinic medication.展开更多
目前地球化学样品分析管理和质量监控系统多为单机版程序。文章介绍基于.net framework 3.5、以Microsoft Visual Studio 2005为开发工具、以Microsoft SQL server 2005为数据库服务器、采用ADO数据库访问技术的地球化学样品分析管理和...目前地球化学样品分析管理和质量监控系统多为单机版程序。文章介绍基于.net framework 3.5、以Microsoft Visual Studio 2005为开发工具、以Microsoft SQL server 2005为数据库服务器、采用ADO数据库访问技术的地球化学样品分析管理和质量监控系统。系统包括基于C/S体系结构的地球化学样品分析管理与质量监控子系统和基于B/S体系结构的分析任务下载与分析结果上传Web子系统,在网络上实现从样品接收、重新编号、分配任务、数据上传到质量监控的所有功能。系统的核心功能是管理地球化学样品分析过程中的任务和数据,进行质量监控。程序调试的结果表明,数据传输和处理准确无误,各功能模块均达到设计要求。展开更多
基金National Natural Science Foundation of China(No.81202865)
文摘Objective To identify the coumarins constituents in Cnidium monnieri and classify ten samples into three groups and this helpful chemical information could be used for the further pharmacological and clinical study on C. monnieri. Methods Qualitative analysis of coumarins in C. monnieri was detected by UHPLC-ESI-Q-TOF/MS. Quadrupole TOF/MS in either full scan mode or extracted ion mode was used for the qualitative analysis of the constituents. Relative peak area of each component was used for the hierarchical cluster analysis. Results According to UHPLC-ESI-QTOF-MS data, chemical structures of 28 coumarins in the fruits of C. monnieri were identified, including 19 simple coumarins, seven linear coumarins, and two angular coumarins. Among these constituents, ten coumarins were firstly identified in C. monnieri. In addition, hierarchical cluster analysis suggested that C. monnieri from different regions could be classified into four groups, and this clustering was correlated to the distribution significantly, Xuzhou could be regarded as the genuine producing area. Conclusion UHPLC-ESI-Q-TOF-MS is a viable method for qualitative analysis and quality evaluation of coumarins from the fruit of C. monnieri. Coumarins in C. monnieri exists intra-species variance, which indicates significant meaning for the quality control and choice of famous region drug for C. monnieri in the clinic medication.
文摘目前地球化学样品分析管理和质量监控系统多为单机版程序。文章介绍基于.net framework 3.5、以Microsoft Visual Studio 2005为开发工具、以Microsoft SQL server 2005为数据库服务器、采用ADO数据库访问技术的地球化学样品分析管理和质量监控系统。系统包括基于C/S体系结构的地球化学样品分析管理与质量监控子系统和基于B/S体系结构的分析任务下载与分析结果上传Web子系统,在网络上实现从样品接收、重新编号、分配任务、数据上传到质量监控的所有功能。系统的核心功能是管理地球化学样品分析过程中的任务和数据,进行质量监控。程序调试的结果表明,数据传输和处理准确无误,各功能模块均达到设计要求。
