The traditional explanation was found to be inconsistent with the empirical evidence that mainly caused the agricultural crisis in 1959-1961, so Yifu Lin tried to explain this catastrophe through using Game Theory bas...The traditional explanation was found to be inconsistent with the empirical evidence that mainly caused the agricultural crisis in 1959-1961, so Yifu Lin tried to explain this catastrophe through using Game Theory based on ＂one-shot game＂. This paper tested the hypothesis through quantitative test. The results showed that system factors mainly resulted in low productivity, and the natural disaster should not be ignored.展开更多
Studying urea formation by ammonolysis of propylene carbonate in a liquid phase showed that this process is two-stage, and proceeds through preferred formation of 2-hydroxypropyl carbamate, besides, the rate of the fi...Studying urea formation by ammonolysis of propylene carbonate in a liquid phase showed that this process is two-stage, and proceeds through preferred formation of 2-hydroxypropyl carbamate, besides, the rate of the first stage is much higher than the rate of the second stage （urea formation）. With the aid of the HPLC and HPLC/MS methods found some other intermediate products and offered consecutive-parallel scheme of the process. The obtained results explain the composition of impurities obtained during the production of the 13C-urea for diagnostic breath tests.展开更多
Objective To develop a universal quantitative immunoenzyme assay (EIA) for detecting amplified products of nucleic acid and its application in hepatit is C virus (HCV). Methods The appropriate cycle number of am...Objective To develop a universal quantitative immunoenzyme assay (EIA) for detecting amplified products of nucleic acid and its application in hepatit is C virus (HCV). Methods The appropriate cycle number of amplification was selected to s top polymerase chain reaction (PCR) before the “plateau stage”. At the same ti me, primers HCV(3) of the second PCR were modified with biotin so that the ampli fied products were labeled. The products were diluted and subsequently added t o the streptavidin coated wells, and the biotinylated products were captured, f ollowed by denaturation of NaOH, and non biotinylated strands were removed. Hy bridization was performed by adding the specific probe labeled with fluorescein. Finally anti fluorescein horse radish peroxidase (HRP) conjugates were added, after washing, 3,3',5,5', tetramethylbenzidine (TMB) was added to the well s and then measured on a microplate reader. Results EIA detection of amplified products of HCV showed that this ass ay was rapid, sensitive, specific and accurate. Correlation between the initial number of viral template and the EIA of amplified products was good. We also pro spectively investigated the response to interferon in five patients with HCV coi nfection. Results showed that this assay could be used as a guidance to the clin ical therapy in directing the use of antiviral drugs. Conclusions This assay could be widely used as a universal technique fo r the quantitative detection of amplified products of all nucleic acid (such as virus, bacterium) and other human genes (such as HLA B 27 ), it has vast vistas.展开更多
基金This paper is supported by National Nature Science Foundation of China （No. 70071041）
文摘The traditional explanation was found to be inconsistent with the empirical evidence that mainly caused the agricultural crisis in 1959-1961, so Yifu Lin tried to explain this catastrophe through using Game Theory based on ＂one-shot game＂. This paper tested the hypothesis through quantitative test. The results showed that system factors mainly resulted in low productivity, and the natural disaster should not be ignored.
文摘Studying urea formation by ammonolysis of propylene carbonate in a liquid phase showed that this process is two-stage, and proceeds through preferred formation of 2-hydroxypropyl carbamate, besides, the rate of the first stage is much higher than the rate of the second stage （urea formation）. With the aid of the HPLC and HPLC/MS methods found some other intermediate products and offered consecutive-parallel scheme of the process. The obtained results explain the composition of impurities obtained during the production of the 13C-urea for diagnostic breath tests.
文摘Objective To develop a universal quantitative immunoenzyme assay (EIA) for detecting amplified products of nucleic acid and its application in hepatit is C virus (HCV). Methods The appropriate cycle number of amplification was selected to s top polymerase chain reaction (PCR) before the “plateau stage”. At the same ti me, primers HCV(3) of the second PCR were modified with biotin so that the ampli fied products were labeled. The products were diluted and subsequently added t o the streptavidin coated wells, and the biotinylated products were captured, f ollowed by denaturation of NaOH, and non biotinylated strands were removed. Hy bridization was performed by adding the specific probe labeled with fluorescein. Finally anti fluorescein horse radish peroxidase (HRP) conjugates were added, after washing, 3,3',5,5', tetramethylbenzidine (TMB) was added to the well s and then measured on a microplate reader. Results EIA detection of amplified products of HCV showed that this ass ay was rapid, sensitive, specific and accurate. Correlation between the initial number of viral template and the EIA of amplified products was good. We also pro spectively investigated the response to interferon in five patients with HCV coi nfection. Results showed that this assay could be used as a guidance to the clin ical therapy in directing the use of antiviral drugs. Conclusions This assay could be widely used as a universal technique fo r the quantitative detection of amplified products of all nucleic acid (such as virus, bacterium) and other human genes (such as HLA B 27 ), it has vast vistas.
