Brain radiomics can reflect the characteristics of brain pathophysiology.However,the value of T1-weighted images,quantitative susceptibility mapping,and R2*mapping in the diagnosis of Parkinson’s disease(PD)was under...Brain radiomics can reflect the characteristics of brain pathophysiology.However,the value of T1-weighted images,quantitative susceptibility mapping,and R2*mapping in the diagnosis of Parkinson’s disease(PD)was underestimated in previous studies.In this prospective study to establish a model for PD diagnosis based on brain imaging information,we collected high-resolution T1-weighted images,R2*mapping,and quantitative susceptibility imaging data from 171 patients with PD and 179 healthy controls recruited from August 2014 to August 2019.According to the inclusion time,123 PD patients and 121 healthy controls were assigned to train the diagnostic model,while the remaining 106 subjects were assigned to the external validation dataset.We extracted 1408 radiomics features,and then used data-driven feature selection to identify informative features that were significant for discriminating patients with PD from normal controls on the training dataset.The informative features so identified were then used to construct a diagnostic model for PD.The constructed model contained 36 informative radiomics features,mainly representing abnormal subcortical iron distribution(especially in the substantia nigra),structural disorganization(e.g.,in the inferior temporal,paracentral,precuneus,insula,and precentral gyri),and texture misalignment in the subcortical nuclei(e.g.,caudate,globus pallidus,and thalamus).The predictive accuracy of the established model was 81.1±8.0%in the training dataset.On the external validation dataset,the established model showed predictive accuracy of 78.5±2.1%.In the tests of identifying early and drug-naïve PD patients from healthy controls,the accuracies of the model constructed on the same 36 informative features were 80.3±7.1%and 79.1±6.5%,respectively,while the accuracies were 80.4±6.3%and 82.9±5.8%for diagnosing middle-to-late PD and those receiving drug management,respectively.The accuracies for predicting tremor-dominant and non-tremor-dominant PD were 79.8±6.9%and 79.1±6.5%,respectively.In conclusion,the multiple-tissue-specific brain radiomics model constructed from magnetic resonance imaging has the ability to discriminate PD and exhibits the advantages for improving PD diagnosis.展开更多
目的:探讨动态增强磁共振成像(DCE-MRI)定量参数联合血清内皮素-1(ET-1)水平检测对子宫肌瘤患者治疗效果的评估价值。方法:回顾性选取2021年6月至2023年6月于郑州市第十五人民医院行子宫肌瘤剔除术患者126例为研究对象,术前1 d、术后3 ...目的:探讨动态增强磁共振成像(DCE-MRI)定量参数联合血清内皮素-1(ET-1)水平检测对子宫肌瘤患者治疗效果的评估价值。方法:回顾性选取2021年6月至2023年6月于郑州市第十五人民医院行子宫肌瘤剔除术患者126例为研究对象,术前1 d、术后3 d均行DCE-MRI检查,并根据术后6个月复发情况将患者分为有效组97例与无效组29例。比较两组术前1 d、术后3 d DCE-MRI定量参数[速率常数(K_(ep))、容量转移常数(K^(trans))、血管外细胞外间隙容积比(V_(e))]及血清ET-1水平,分析DCE-MRI定量参数、ET-1水平与治疗效果的相关性,并分析联合应用对治疗效果的评估价值。结果:无效组术后3 d K_(ep)、K^(trans)、V_(e)值及血清ET-1水平高于有效组(P<0.05);术后3 d DCE-MRI定量参数K_(ep)、K^(trans)、V_(e)值及血清ET-1水平与子宫肌瘤患者治疗效果均呈正相关,且联合评估治疗无效的曲线下面积(area under curve,AUC)为0.897,最佳预测敏感度、特异度分别为87.63%、82.76%。结论:DCE-MRI定量参数联合血清ET-1水平对子宫肌瘤治疗效果具有一定评估价值,可作为临床早期评估治疗效果的有效方案之一。展开更多
目的探讨距骨骨软骨损伤(osteochondral lesions of the talus,OLT)软骨基质金属蛋白酶-1(matrix metalloproteinase-1,MMP-1)的表达与磁共振T2值之间的关系及其在距骨骨软骨损伤的机制。材料与方法收集30例踝关节距骨骨软骨损伤行微创...目的探讨距骨骨软骨损伤(osteochondral lesions of the talus,OLT)软骨基质金属蛋白酶-1(matrix metalloproteinase-1,MMP-1)的表达与磁共振T2值之间的关系及其在距骨骨软骨损伤的机制。材料与方法收集30例踝关节距骨骨软骨损伤行微创治疗术患者及30名健康志愿者,均行3.0 T常规踝关节磁共振成像(magnetic resonance imaging,MRI)及T2-mapping功能成像,测量距骨软骨T2值。术后离体距骨骨软骨行苏木精-伊红染色(hematoxylin-eosin staining,HE)和Western Blot法分析距骨骨软骨MMP-1的表达,统计学分析距骨骨软骨T2值与MMP-1蛋白表达的相关性。结果距骨骨软骨损伤以内中侧最为多见(磁共振6分区法),距骨骨软骨T2值随软骨损伤Mankin分级明显增大(P<0.05)。软骨MMP-1蛋白表达高级别与低级别差异明显(P<0.05)。软骨T2值与软骨MMP-1表达呈线性相关趋势,Pearson分析距骨骨软骨损伤T2值增加与MMP-1蛋白表达增加呈直线性正相关(r=0.821,P<0.0001)。结论距骨骨软骨损伤以内中侧最为多见,距骨骨软骨磁共振T2值随软骨损伤分级逐级递增;距骨骨软骨损伤磁共振T2值与MMP-1蛋白表达呈正相关,距骨骨软骨磁共振T2-mapping功能成像T2值定量分析可在体、无创性预测不同级别软骨损伤MMP-1蛋白的表达含量,为临床治疗OLT提供影像学定量依据。展开更多
建立了核磁共振氟谱法测定氰化物的氟化衍生化合物1-氰基-2,2,2-三氟-1-苯乙基乙酸酯(CTPA)含量的方法。以乙酸3-氟苄酯为内标,采用5mm Auto XBB探头,探头温度298.2K,考察了不同浓度化合物的定量条件;通过比较化合物样品定量峰与内标物...建立了核磁共振氟谱法测定氰化物的氟化衍生化合物1-氰基-2,2,2-三氟-1-苯乙基乙酸酯(CTPA)含量的方法。以乙酸3-氟苄酯为内标,采用5mm Auto XBB探头,探头温度298.2K,考察了不同浓度化合物的定量条件;通过比较化合物样品定量峰与内标物质响应峰面积,计算化合物的含量。结果该化合物的19 F-NMR检测方法在0.5~50μg/mL具有良好的线性(r2=0.9966),该方法的定量限0.5μg/mL,检出限0.2μg/mL;同时考察了该化合物在不同浓度下的测量准确度。展开更多
建立食用醋中乙酸含量的快速定量分析方法。采用核磁共振氢谱(^(1)H nuclear magnetic resonance,^(1)H NMR),对多种食用醋进行了测定。核磁共振氢谱(^(1)H-NMR)测定参数为:温度25℃,谱宽2~14ppm,脉冲角度45度,脉冲延迟时间3s,采样时间...建立食用醋中乙酸含量的快速定量分析方法。采用核磁共振氢谱(^(1)H nuclear magnetic resonance,^(1)H NMR),对多种食用醋进行了测定。核磁共振氢谱(^(1)H-NMR)测定参数为:温度25℃,谱宽2~14ppm,脉冲角度45度,脉冲延迟时间3s,采样时间2s,扫描次数16次。实验结果表明,在优化的测试条件下,目标物与内标物定量特征峰面积比1∶3~4∶1范围内,该方法表现出良好的线性关系,线性回归方程为Y=-0.01996+0.01732X(R=0.9999);精密度和重复性实验相对标准偏差(RSD)分别为0.059%和0.249%;样品加标回收率为97.8%~101.4%,平均偏差0.9%。该方法样品需求量小,结果稳定可靠,是一种简便、快捷的食用醋中乙酸含量的检测方法。展开更多
基金supported by the National Natural Science Foundation of China, Nos.82001767(to XJG), 81971577(to MMZ), 82171888(to XJX)the Natural Science Foundation of Zhejiang Province of China, Nos.LQ21H180008(to XJG), LQ20H180012(to MX)+1 种基金the China Postdoctoral Science Foundation, Nos.2021T140599(to XJG), 2019M662082(to XJG)the 13th Five-year Plan for National Key Research and Development Program of China, No.2016YFC1306600(to MMZ)
文摘Brain radiomics can reflect the characteristics of brain pathophysiology.However,the value of T1-weighted images,quantitative susceptibility mapping,and R2*mapping in the diagnosis of Parkinson’s disease(PD)was underestimated in previous studies.In this prospective study to establish a model for PD diagnosis based on brain imaging information,we collected high-resolution T1-weighted images,R2*mapping,and quantitative susceptibility imaging data from 171 patients with PD and 179 healthy controls recruited from August 2014 to August 2019.According to the inclusion time,123 PD patients and 121 healthy controls were assigned to train the diagnostic model,while the remaining 106 subjects were assigned to the external validation dataset.We extracted 1408 radiomics features,and then used data-driven feature selection to identify informative features that were significant for discriminating patients with PD from normal controls on the training dataset.The informative features so identified were then used to construct a diagnostic model for PD.The constructed model contained 36 informative radiomics features,mainly representing abnormal subcortical iron distribution(especially in the substantia nigra),structural disorganization(e.g.,in the inferior temporal,paracentral,precuneus,insula,and precentral gyri),and texture misalignment in the subcortical nuclei(e.g.,caudate,globus pallidus,and thalamus).The predictive accuracy of the established model was 81.1±8.0%in the training dataset.On the external validation dataset,the established model showed predictive accuracy of 78.5±2.1%.In the tests of identifying early and drug-naïve PD patients from healthy controls,the accuracies of the model constructed on the same 36 informative features were 80.3±7.1%and 79.1±6.5%,respectively,while the accuracies were 80.4±6.3%and 82.9±5.8%for diagnosing middle-to-late PD and those receiving drug management,respectively.The accuracies for predicting tremor-dominant and non-tremor-dominant PD were 79.8±6.9%and 79.1±6.5%,respectively.In conclusion,the multiple-tissue-specific brain radiomics model constructed from magnetic resonance imaging has the ability to discriminate PD and exhibits the advantages for improving PD diagnosis.
文摘目的:探讨动态增强磁共振成像(DCE-MRI)定量参数联合血清内皮素-1(ET-1)水平检测对子宫肌瘤患者治疗效果的评估价值。方法:回顾性选取2021年6月至2023年6月于郑州市第十五人民医院行子宫肌瘤剔除术患者126例为研究对象,术前1 d、术后3 d均行DCE-MRI检查,并根据术后6个月复发情况将患者分为有效组97例与无效组29例。比较两组术前1 d、术后3 d DCE-MRI定量参数[速率常数(K_(ep))、容量转移常数(K^(trans))、血管外细胞外间隙容积比(V_(e))]及血清ET-1水平,分析DCE-MRI定量参数、ET-1水平与治疗效果的相关性,并分析联合应用对治疗效果的评估价值。结果:无效组术后3 d K_(ep)、K^(trans)、V_(e)值及血清ET-1水平高于有效组(P<0.05);术后3 d DCE-MRI定量参数K_(ep)、K^(trans)、V_(e)值及血清ET-1水平与子宫肌瘤患者治疗效果均呈正相关,且联合评估治疗无效的曲线下面积(area under curve,AUC)为0.897,最佳预测敏感度、特异度分别为87.63%、82.76%。结论:DCE-MRI定量参数联合血清ET-1水平对子宫肌瘤治疗效果具有一定评估价值,可作为临床早期评估治疗效果的有效方案之一。
文摘建立了核磁共振氟谱法测定氰化物的氟化衍生化合物1-氰基-2,2,2-三氟-1-苯乙基乙酸酯(CTPA)含量的方法。以乙酸3-氟苄酯为内标,采用5mm Auto XBB探头,探头温度298.2K,考察了不同浓度化合物的定量条件;通过比较化合物样品定量峰与内标物质响应峰面积,计算化合物的含量。结果该化合物的19 F-NMR检测方法在0.5~50μg/mL具有良好的线性(r2=0.9966),该方法的定量限0.5μg/mL,检出限0.2μg/mL;同时考察了该化合物在不同浓度下的测量准确度。
文摘建立食用醋中乙酸含量的快速定量分析方法。采用核磁共振氢谱(^(1)H nuclear magnetic resonance,^(1)H NMR),对多种食用醋进行了测定。核磁共振氢谱(^(1)H-NMR)测定参数为:温度25℃,谱宽2~14ppm,脉冲角度45度,脉冲延迟时间3s,采样时间2s,扫描次数16次。实验结果表明,在优化的测试条件下,目标物与内标物定量特征峰面积比1∶3~4∶1范围内,该方法表现出良好的线性关系,线性回归方程为Y=-0.01996+0.01732X(R=0.9999);精密度和重复性实验相对标准偏差(RSD)分别为0.059%和0.249%;样品加标回收率为97.8%~101.4%,平均偏差0.9%。该方法样品需求量小,结果稳定可靠,是一种简便、快捷的食用醋中乙酸含量的检测方法。
