Dry Breeding and Dry Planting Techniques for Indica Hybrid Rice in Karst Mountain Areas of Gejiu City

Based on the arable land situation in Gejiu City,upland dry planting of indica hybrid rice is being expanded in Karst mountain areas with a rainfall of over 1400 mm and an altitude of 1100-1600 m to develop grain production.This paper gives a specific description of hybrid rice upland dry seedling technology,upland transplanting technology,fertilization technology,field management,weed prevention and control technology,and disease and pest control.

Grain yield,nitrogen use efficiency and physiological performance of indica/japonica hybrid rice in response to various nitrogen rates

Utilizing the heterosis of indica/japonica hybrid rice(IJHR)is an effective way to further increase rice grain yield.Rational application of nitrogen(N)fertilizer plays a very important role in using the heterosis of IJHR to achieve its great yield potential.However,the responses of the grain yield and N utilization of IJHR to N application rates and the underlying physiological mechanism remain elusive.The purpose of this study was to clarify these issues.Three rice cultivars currently used in rice production,an IJHR cultivar Yongyou 2640(YY2640),a japonica cultivar Lianjing 7(LJ-7)and an indica cultivar Yangdao 6(YD-6),were grown in the field with six N rates(0,100,200,300,400,and 500 kg ha^(-1))in 2018 and 2019.The results showed that with the increase in N application rates,the grain yield of each test cultivar increased at first and then decreased,and the highest grain yield was at the N rate of 400 kg ha^(-1)for YY2640,with a grain yield of 13.4 t ha^(-1),and at 300 kg ha^(-1)for LJ-7 and YD-6,with grain yields of 9.4–10.6 t ha^(-1).The grain yield and N use efficiency(NUE)of YY2640 were higher than those of LJ-7 or YD-6 at the same N rate,especially at the higher N rates.When compared with LJ-7 or YD-6,YY2640 exhibited better physiological traits,including greater root oxidation activity and leaf photosynthetic rate,higher cytokinin content in the roots and leaves,and more remobilization of assimilates from the stem to the grain during grain filling.The results suggest that IJHR could attain both higher grain yield and higher NUE than inbred rice at either low or high N application rates.Improved shoot and root traits of the IJHR contribute to its higher grain yield and NUE,and a higher content of cytokinins in the IJHR plants plays a vital role in their responses to N application rates and also benefits other physiological processes.

Dry matter production and panicle characteristics of high yield and good taste indica hybrid rice varieties

Indica hybrid rice(Oryza sativa) production aims to achieve two crucial targets: high yield and good taste. This study selected three types of indica hybrid rice according to grain yield and taste value, including high yield and good taste(HYGT), low yield and good taste(LYGT), and high yield and poor taste(HYPT), to analyze yield components,corresponding growth characteristics, and rice taste quality. When values were averaged across varieties and years,there were almost no differences in taste value between HYGT and LYGT;HYGT showed a significant increase in yield,owing to a higher number of panicles and spikelets per panicle, with a respective increment of 16.2 and 20.6%. The higher grain yield of HYGT compared with LYGT was attributed to three key factors: a higher leaf area index(LAI) during heading, a higher ratio of grain to leaf, and a higher biomass accumulation at maturity. HYGT and HYPT achieved similar high yields;however, HYGT had more panicle numbers and lower grain weight. In addition, HYGT showed a significantly higher taste value than HYPT, attributed to its significantly lower protein and amylose contents, with reductions of 8.8and 15.7%, respectively. Lower protein and amylose contents might be caused by a proper matter translocation from vegetative organs to panicle. This study suggested that reasonable panicle characteristics and translocation efficiency from vegetative organs to panicle during heading to maturity are the key factors in balancing yield and rice taste quality.These results will provide valuable insights for rice breeders to improve the grain yield and quality of indica hybrid rice.

Mitigating risks of hybrid rice use in terrace agriculture

The Honghe Hani Rice Terraces of Yunnan Province have become a national emblem for China and a UNESCO World Heritage Site,but some are beginning to crumble.This research attempts to address why this is happening and what can be done about it.Previous work has failed to adequately address the possible shortcomings of recently introduced seed and water management technologies,their particular effect on rice terraces and the people who depend upon them.In an effort to better understand the issue,field observation was triangulated with in-depth interviews with local people,and the examination of scientific literature.To do this,the authors spent time in China with a translator in a key village known to be first in the area to truly succeed in carving the rice terraces and in making the mountain slope irrigation system required actually working.Results validated by experts in each field indicate that while the new seed and technologies do save water and improve lives,paired with migration,they may also be threatening the long-term viability of rice terracing in the region.The authors conclude that an integrated approach is needed and put forward a strategic blueprint to reinforce ecological,social and economic longevity.They also call for more research into the applicability of these resilience measures in other hybrid rice terracing regions,such as in the Philippines and Vietnam.

Irrigating with cooler water does not reverse high temperature impact on grain yield and quality in hybrid rice

Rice grain yield and quality are negatively impacted by high temperature stress.Irrigation water temperature significantly affects rice growth and development,thus influencing yield and quality.The role of cooler irrigation water in counteracting high temperature induced damages in rice grain yield and quality are not explored.Hence,in the present study two rice hybrids,Liangyoupeijiu(LYPJ)and IIyou 602(IIY602)were exposed to heat stress and irrigated with water having different temperatures in a splitsplit plot experimental design.The stress was imposed starting from heading until maturity under field-based heat tents,over two consecutive years.The maximum day temperature inside the heat tents was set at 38℃.For the irrigation treatments,two different water sources were used including belowground water with cooler water temperature and pond water with relatively higher water temperature.Daytime mean temperatures in the heat tents were increased by 1.2–2.0℃ across two years,while nighttime temperature remained similar at both within and outside the heat tents.Cooler belowground water irrigation did have little effect on air temperature at the canopy level but decreased soil temperature(0.2–1.4℃)especially under control.Heat stress significantly reduced grain yield(33%to 43%),panicles m^(-2)(9%to 10%),spikelets m^(-2)(15%to 22%),grain-filling percentage(13%to 26%)and 1000-grain weight(3%to 5%).Heat stress significantly increased chalkiness and protein content and decreased grain length and amylose content.Grain yield was negatively related to air temperature at the canopy level and soil temperature.Whereas grain quality parameters like chalkiness recorded a significantly positive association with both air and soil temperatures.Irrigating with cooler belowground water reduced the negative effect of heat stress on grain yield by 8.8%in LYPJ,while the same effect was not seen in IIY602,indicating cultivar differences in their response to irrigation water temperature.Our findings reveal that irrigating with cooler belowground water would not significantly mitigate yield loss or improve grain quality under realistic field condition.The outcome of this study adds to the scientific knowledge in understanding the interaction between heat stress and irrigation as a mitigation tool.Irrigation water temperature regulation at the rhizosphere was unable to counteract heat stress damages in rice and hence a more integrated management and genetic options at canopy levels should be explored in the future.

Hyperparameter Optimization for Capsule Network Based Modified Hybrid Rice Optimization Algorithm

Hyperparameters play a vital impact in the performance of most machine learning algorithms.It is a challenge for traditional methods to con-figure hyperparameters of the capsule network to obtain high-performance manually.Some swarm intelligence or evolutionary computation algorithms have been effectively employed to seek optimal hyperparameters as a com-binatorial optimization problem.However,these algorithms are prone to get trapped in the local optimal solution as random search strategies are adopted.The inspiration for the hybrid rice optimization(HRO)algorithm is from the breeding technology of three-line hybrid rice in China,which has the advantages of easy implementation,less parameters and fast convergence.In the paper,genetic search is combined with the hybrid rice optimization algorithm(GHRO)and employed to obtain the optimal hyperparameter of the capsule network automatically,that is,a probability search technique and a hybridization strategy belong with the primary HRO.Thirteen benchmark functions are used to evaluate the performance of GHRO.Furthermore,the MNIST,Chest X-Ray(pneumonia),and Chest X-Ray(COVID-19&pneumonia)datasets are also utilized to evaluate the capsule network learnt by GHRO.The experimental results show that GHRO is an effective method for optimizing the hyperparameters of the capsule network,which is able to boost the performance of the capsule network on image classification.

Effects of Different Seed Coating Agents on the Quality of"Grey-matter"Hybrid Rice Seeds

[Objectives]This study was conducted to select suitable mixed seed coating agents for improving the quality of"grey-matter"hybrid rice seeds.[Methods]Three different mixed seed coating agents(A,B,C)were used to coat the seeds of two varieties(Taiyou 390 and Zhenliangyou 8612)of hybrid rice with different"grey-matter"content(5%,15%,25%),and the results were investigated and compared.[Results]The combinations of treatment B(seed coating agent A+Linong)and treatment C(Manshijin+seed coating agent A)could significantly improve indexes including seed germination potential,germination rate,seed vigor,seedling height,fibrous roots and fresh weight of the two varieties with a"grey-matter"content greater than 15%,but had no significant effects on main root length,dry weight,leaf number and tiller number,and the effects of treatment B was better than those of treatment C.That is to say,mixed seed coating agent B(seed coating agent A+Linong)was suitable for use as a seed coating agent to improve the quality of"grey-matter"seeds.[Conclusions]This study provides a reference plan for the safe use of mixed seed batches with"grey-matter"deterioration.

Breeding and Seed Production Techniques of New Aromatic Danxiangyouzhenliang Rice Variety with Good Quality and High Yield

Danxiangyouzhenliang rice is a high-quality rice variety derived from the hybrid breeding of Danxiang 12A and Xianghuixiangmiao R133.Its unique temperature-sensitive three-line characteristics endow it with significant advantages of high and stable yield,and its rice fragrance is exquisite and its taste is delicate.Danxiangyouzhenliang rice showed good resistance to rice blast and bacterial blight.On June 10,2021,Danxiangyouzhenliang rice passed the Guangxi Crop Variety Approval(Guishendao 2021074).Danxiangyouzhenliang rice is suitable to be planted as early and late rice in southern,central and northern Guangxi.This study summarized the breeding process and high-yielding seed production techniques of Danxiangyouzhenliang rice,in order to provide useful reference for rice breeders and growers.

Breeding and Application Techniques of a New Rice Variety Liliang-you 3822 with High Yield and Disease Resistance

Liliangyou 3822 is a novel indica hybrid rice variety that exhibits disease resistance,high yield,lodging resistance,and late maturity.It employs a self-selected two-line sterile line,Li 38S,and a self-selected restorer line,R22.This variety was subjected to a regional test of indica late-maturing groups in the middle and lower reaches of the Yangtze River in 2020.The results demonstrated that the average yield of the variety was 9.95 t/hm 2,which was 10.67%higher than that of the control Fengliangyou 4,indicating a highly significant yield increase.In the continuous test in 2021,the average yield was 9.74 t/hm 2,representing a 6.52%increase over the control,which also exhibited a significant increase.Finally,the average yield of the two years regional test was 9.84 t/hm 2,which was 8.58%higher than that of the control.In the 2021 production test,the average yield of the variety was 9.32 t/hm 2,which was 12.19%higher than that of the control,indicating a remarkably significant yield increase.In 2022,the variety was validated by the National Crop Variety Approval Committee(GSD 20220143).

Photosynthetic Characteristics of Two Superhigh-yield Hybrid Rice

The photosynthetic functions and the sensitivity to photoinhibition were compared between two superhigh_yield hybrid rice (Oryza sativa L.) Liangyoupeijiu and X07S/Zihui 100, the newly developed from two parental lines and traditional hybrid rice Shanyou 63 developed from three parental lines. The results showed that, as compared to Shanyou 63, the net photosynthetic rate of Liangyoupeijiu and X07S/Zihui 100 was 9.1% and 11.9% higher, the transpiration rate was 37.4% and 31.4% lower, and their water use efficiency was 74.2% and 63.5% higher respectively. After strong light (2 000 μmol photons·m -2 ·s -1 ) treatment for 2 h, the photochemical quantum yield and the photochemical quenching increased by 37.0% and 18.0% respectively in Liangyoupeijiu, 28.3% and 46.2% in X07S/Zihui 100, but decreased a little in Shanyou 63. The non_photochemical quenching decreased in Liangyoupeijiu and X07S/Zihui 100 (about 50%) but increased greatly in Shanyou 63 (about 50%). Better photosynthetic functions, higher water use efficiency and stronger resistance to photoinhibition, may be the physiological basis for the super high_yield of the two hybrid rice under study.

Study on Mechanization Hybrid Rice Seed Production Technology and Combine Breeding Advances

[Objectives]Current hybrid rice seed production mechanization and a new kind of hybrid rice seed production mechanization using Bentazone sensitive lethal genes for mechanized seed production technology are introduced in this paper.[Method]Sensitive Restorer Bentazone lethal dose of filtering,parental and seed weight are bred combinations of several aspects of the system described,and presented mechanized seed technology new research directions.[Results]The method of using Bentazone sensitive lethal genes for mechanized seed production technology is feasible,economic,seed production and combination advantages.[Conclusions] Using Bentazone sensitive lethal genes for mechanized seed production technology is the new study direction of mechanic production.

Correlation Analysis of Characters of Indica Hybrid Rice with High Content of Amylose

[Objective] The aim was to research and analyze characters and related correlation of indica hybrid rice containing high amylose. [Method] In the research, major characters of 16 species of groups of {ndica hybrid rice and the correlation were explored in rice belts in Sichuan in 2011. [Result] The variation of chalkiness degree was the highest at 26.8%, followed by rate of head rice at 22.89%; varia- tions of head rice rate and chalkiness degree were lower at 0.72% and 2.61%. In addition, rate of unpolished rice was of significantly negative correlation with chalki- ness degree; rate of chalky rice was of extremely positive correlation with chalkiness degree and gel consistency; chalkiness degree was of significantly positive correla- tion with gel consistency; gel consistency was of significantly negative correlation with protein content; amylose content was of insignificant correlation with gel consis- tency （r=0.166 9） of the 16 hybrid groups. These indicated that gel consistency would be still high as the content of amylose grows （AC≥25%）. For example, when the content of anylose in Chuanxiang 29A/Luhui 37, Kangfeng AJ6684, and Lefeng A/SR536 exceeded 27%, the gel consistency was over 80 mm in the research. [Conclusion] The research provides references for improvement of amylose content in breeding hybrid rice.

Effects of Tiller-inhibitor on Growth and Yield Formation of Super Early Hybrid Rice Jinyou458

[Objective]The paper was to study the effect of tiller-inhibitor on the growth and yield formation of super early rice Jinyou 458.[Method] Two treatments including spraying tiller-inhibitor（TI） and spraying water（CK） at the SN-n stage were set in the test,the effect of tiller-inhibitor on yield and its components,leaf and plant morphology,field microclimate at booting stage and quality characteristics of rice population were studied.[Result] Spraying tiller-inhibitor could effectively reduce the occurrence of invalid and inefficient tillers,increase the proportion of high effective tillers（tiller with 4 or more leaves） in tiller composition at the maximal tiller stage.The panicle length,spikelets per panicle,spikelets density,number of secondary branches and the secondary spikelets,seed setting rate of rice plant sprayed with tiller-inhibitor were significantly higher than CK.Meanwhile,spraying tiller-inhibitor could increase plant height,biomass weight and leaf area index of rice population during middle and late stages of rice growing,improve the leaf temperature of top three leaves and the light transmittance of rice population at the booting stage,increase the leaf SPAD value,thus enhance photosynthetic capacity of rice plants.It also showed the characteristics of elongating the internode of rice plant and decreasing the stem thickness,etc.[Conclusion] The panicle-bearing rate of stems and tillers,effective panicles,spikelets per panicle,seed setting rate and 1 000-grain weight had coordinately increased after spraying tiller-inhibitor,thus increased the yield.

Breeding of New Japonica-indica Hybrid Rice Combination——Chunyou 618

[Objective] The aim was to select and breed Chunyou 618 which is a novel combination among japonica-indica hybrid rice subspecies. [Method] Chunyou 618 is a japonica-indica hybrid rice combination developed from Chunjiang 16A, a late japonica CMS line with dwarfness and early anthesis time, and C18, an indicaclinous wide compatibility restorer line. In addition, the concerning characters were researched. [Result] Chunyou 618, which is japonica inclining, proved high in pro- duction and in resistance to lodging with Cheng＇s index at 15. It is of moderate re- sistance against Xanthomonas oryzae and stripe virus disease, of moderate susceptibility on rice blast, and of susceptibility on Nilaparvata lugens. The indices of rice quality achieved departmental standard 4 and grade 4 of Cooking Rice Variety Quality, respectively. [Conclusion] Chunyou 618 enjoys strong heterosis, strong stem, good lodging resistance, large panicles, good cold tolerance, high yield and wide adaptability, which is suitable to be grown in Zhejiang Province as single cropping late rice.

Research on Yield and Quality of Hybrid Rice under Efficient Application Amount of Phosphorus and Potassium

[Objective] Application of P and K was not reasonable in southwest rice producing area, with no effective method for guiding application of P and K. This study aimed to conduct experiments with the same N application and different P and K application in different ecological points, to explore a scientific and efficient management method of P and K nutrients and provide guidance for application of P and K in rice production. [Method] Hybrid rice Chuanxiang9838 was used as experimental material to study the relationship among application amount of P and K, utilization rates of P and K, rice yield and rice quality in seven ecological points of four provinces （municipalities） including Sichuan, Chongqing, Yunnan and Guizhou. The application amount per hectare of P was 37.5, 75, 112.5 and 150 kg, respectively; the application amount per hectare of K was 45, 135 and 180 kg, respectively. A total of 10 treatments were set for split-plot design with three replications. [Result] Yield of Chuanxiang9838 significantly varied in different experimental locations and under different levels of P and K, interaction of ecological point and different P and K treatments varied significantly. Yield had reached the maximum in ecological point of Yunnan Binchuan and in treatment with application of N 150 kg P 75 kg K 90 kg. Efficient utilization rates of P and K varied in each ecological point, efficient application amount of P and K in Sichuan Dongpo, Sichuan Luxian, Guizhou Xiaohe and Chongqing Yongchuan was the same, while that in Sichuan Guanghan, Sichuan Zhongjiang and Yunnan Binchuan was different. The main objective of high-yield rice production was to increase the number of effective panicles per hectare and seed setting rate. Rice quality was clustered into 2 categories by locations and 6 categories by treatments. [Conclusion] Prediction models of efficient application of P and K in rice production were established, which provided guidance for high-yield rice production and rational and efficient utilization of P and K in southwest rice area.

Relationship between Leaf Area Index and Yield of Hybrid Rice

In the research, 20 indica hybrid rice varieties were investigated under high-dense and low-amount, mid-dense and mid-amount and low-dense and high-amount fertilization conditions in order to explore correlation between LAI in ful heading stage with yield and to analyze yield differences, yield components, material accumulation and transferring, as wel as correlation between key cultivation factors and yield. Final y, approaches and key technology for yield breakthrough were dis-cussed, which provides theoretical and technological references for rice high yield.

The Principal Component Analysis on Yielding and Agronomic Traits of Hybrid Rice of Liangyou 2111

In order to define the relationship between yield and important agronomic traits of two lines hybrid Uangyou 2111, the principal component analysis method was used to analyze the expadmental data of six test points in Yunnan Province. The results showed that the main factors influencing the production of Liangyou 2111 were grain number, grains seed number, panicle length, growth padod and panicle rate; then were 1 O00-grain weight, seed setting rate, effective panicle and highest stem tillers number; again was plant height. Therefore, when hybrid rice of Uangyou 2111 will be planted widely in yunnan province, we should focus on en- sudng the panicle traits, especially increase grain number and grain seed number, and coordinately develop other traits to achieve high yield.

Correlations between Drought Resistance and Shoot Traits of Mid-season Hybrid Rice

[Objective] This study aimed to investigate the correlations between the drought tolerance and plant traits of hybrid rice, so as to provide theoretical basis for the breeding of drought-tolerant rice varieties. [Method] In the field experiment in 2011, 30 hybrid rice cultivars were grown under three different conditions： drought at til ering stage, drought at panicle initiation stage and control （keeping shal ow wa-ter during the whole growth period）. Then, the main plant traits were measured, and the related drought tolerance indices were calculated. In 2012, 16 hybrid rice culti-vars were grown in pots under drought and normal water conditions respectively. And their main plant traits were measured, and the related drought tolerance indices were also calculated. [Result] The water content in soil under drought stress at til ering stage was nearly 60%, and that under drought stress at panicle initiation stage was 80%. Such low water content significantly reduced the rice yield. The cultivars with large panicle and great root growth potential had strong drought toler-ance at panicle initiation stage. Under drought stress, the cultivars with higher yield had stronger tolerance to drought. The drought tolerance indices at til ering stage had no correlations with those at panicle initiation stage. Under normal water condi-tions, the cultivars with smal er 1 000-grain weight had stronger tolerance to drought . Under drought stress , the cultivars with smal er 1 000-grain weight and higher grain yield had stronger tolerance to drought. [Conclusion] Among al the rice cultivars tested in the present study, D You 6511, Taiyou 99, Nei 5 You 317, Neixiangyou 18, Yixiangyou 7633, Tianyouhuazhan, II You 615, Neixiang 5306, Chuanguyou 7329 and Neixiang 7539 exhibited better tolerance to drought, and thus can be promoted in production.

Variation of Nitrogen Uptake and Utilization Efficiency of Mid-Season Hybrid Rice at Different Ecological Sites under Different Nitrogen Application Levels

[Objective] The study aimed at investigating the effects of different geographic sites,soil chemical characteristics and nitrogen application levels on nitrogen accumulation and distribution in different organs and utilization efficiency for mid-season hybrid rice.[Method] By using mid-season rice varieties II-you 7 and Yuxiangyou203 as the experimental materials,field experiment was conducted at seven ecological sites in four provinces or cities in Southwestern China in 2009.A total of four nitrogen application levels were set as follows：by using 75 kg/hm2 of P2O5 and 75 kg/hm2 of K2O as the base fertilizer,extra 0,90,150 and 210 kg/hm2 of nitrogen fertilizer（in which,base fertilizer,base-tillering fertilizer and base-earing fertilizer respectively accounted for 60%,20% and 20%.） was applied,respectively.In the split-plot design,fertilizer was considered as the main factor while rice variety was taken as the secondary factor.A total of eight treatments were set with three replications.[Result] Highly significant differences of grain yield were found among seven locations,two varieties,four nitrogen application levels,interactions of site × variety and site × nitrogen application level,but the interaction of variety ×nitrogen application level had no significant influence on rice yield.There were highly significant effects of site,varieties and nitrogen application level on dry matter production,nitrogen content,nitrogen utilization efficiency.Highly significant negative correlations between uptake efficiency and utilization efficiency for nitrogen were found;and multiple stepwise regression analysis showed that nitrogen uptake-utilization efficiency were significantly influenced by different ecological sites,chemical quality of soil and the levels of nitrogen application.[Conclusion] The research will provide theoretical and practical basis for the highly efficient application of nitrogen in mid-season hybrid rice cultivation.

Identification of Phosphorus Starvation Inducible Genes in Rice by Suppression Subtractive Hybridization

Phosphorus is one of the three essential macroelements for plant growth. Plants respond to phosphorus starvation through adaptive mechanisms involved in morphological, biochemical and molecular changes. To investigate the molecular background of the adaptive mechanisms, the suppression subtractive hybridization (SSH) method was used to construct a rice phosphorus-starvation ( Pi-starvation) induced cDNA library. Through screening of the cDNA library and sequencing of the enriched cDNAs, 18 known genes and 47 novel genes were identified. The known genes are involved in different metabolic processes, including phosphate uptake and transport, signal transduction, protein synthesis and degradation, carbon metabolism and stress response. Northern analysis was performed to detect the expression patterns of some known genes and novel genes under different phosphorus levels. Different expression patterns of the selected genes were identified, which suggests that genes involved in different pathways may have different responses to Pi-starvation.