Objective： The present study was designed to investigate whether quercetin exerts increasing chemosensitivity on human lung adenocarcinoma cells when quercetin combined with cisplatin （DDP） and vincristine （VCR） ...Objective： The present study was designed to investigate whether quercetin exerts increasing chemosensitivity on human lung adenocarcinoma cells when quercetin combined with cisplatin （DDP） and vincristine （VCR） in vitro respectively and its possible antitumor mechanism. To provide experimental proof for clinical combination application. Methods： Using intermittent administration of high dose VCR, human lung adenocarcinoma sensitive cell line （A549/S） was induced to VCR- resistant human lung adenocarcinoma cell line （A549NCR）. MTT assay was adapted for examing the 50% inhibition （IC50） value of DDP and VCR on A549/S and A549/VCR when quercetin combined with DDP and VCR respectively. Results： IC50 of DDP on A549/S and A549/VCR was 10.18 and 12.35 mg/L, and the IC50 of VCR on the two cell lines was 1.21 and 12.77 rag/L, respectively. The resistance fold of A549/VCR on VCR and DDP was 10.55 and 121, respectively. When quercetin at concentration of 50, 100 and 200 pmol/L in combination with DDP and VCR respectively, the IC50 of DDP and VCR on A549/S and A549/VCR were obvious decreased （P 〈 0.05 - P 〈 0.01）. Conclusion： The experiment results suggested that quercetin could increase the chemosensitivity and partly revise the resistance of A549NCR.展开更多
Introduction: The aim of this study is to test the factorial structure and the internal consistency of the 12-items General Health Questionnaire adapted to work-related psychological distress (GHQW). Methods: A valida...Introduction: The aim of this study is to test the factorial structure and the internal consistency of the 12-items General Health Questionnaire adapted to work-related psychological distress (GHQW). Methods: A validated French version of the GHQ-12 was used and transformed to remind of the occupational context. A sample of 1014 workers completed the GHQW. Internal consis- tency was assessed by Cronbach’s α coefficient. The factorial structure was extracted with an exploratory factorial analysis (EFA). Results: The EFA run on the data yield to a one- factor structure explaining 60.5% of the total variance of the scale. The Cronbach’s alpha showed a very good internal consistency of the scale (α = 0.94). Conclusion: The findings support that the GHQW is a reliable and valid instrument for measuring work-related psychological distress in workers. This work-related version could find some applications in epide-miological research at work, in the study of psychosocial risk factors and in the occupational health physi- cian’s daily activity.展开更多
En francais moderne,on constate une extension de l'emploi de “le fait que”.Cela tient au fait que cet emploi facilite beaucoup la construction de certaines phrases.L'emploi de “le fait que” est divers,
Objective To study the effects of different concentrations of Quercetin on nitric oxide (NO) production and membrane fluidity of the injured human umbilical vein vascular endothelial cell line(ECV 304) with hypoxia...Objective To study the effects of different concentrations of Quercetin on nitric oxide (NO) production and membrane fluidity of the injured human umbilical vein vascular endothelial cell line(ECV 304) with hypoxia and the lack of glucose. Methods The experiments were performed in the culture of ECV 304 injured with hypoxia and the lack of glucose in vitro. The releases of intracellular lactate dehydrogenase(LDH) of ECV 304 was measured with automatic biochemistry analysis. NO level of ECV 304 was monitored with colorimetry. The membrane fluidity of ECV 304 was measured with the fluorescence polarization method. Results After ECV 304 was cultured in hypoxia and the the lack of glucose for 24 hours, the release of LDH and the membrane fluidity were increased significantly; NO level was decreased. Preincubation of ECV 304 with 20, 80,160 μ mol·L -1 of Quercetin for 24 hours reduced LDH activity, membrane fluidity and increased the level of NO in hypoxia and the lack of glucose induced ECV 304. Conclusion These results demonstrate that Quercetin can produce the protective effect on hypoxia and the lack of glucose induced injury of ECV 304 by increasing release of NO and changing membrane fluidity.展开更多
基金Supported by a grant from the Health Department of Jiangxi Province for Science Technical Program Foundation(No.2005-22A)
文摘Objective： The present study was designed to investigate whether quercetin exerts increasing chemosensitivity on human lung adenocarcinoma cells when quercetin combined with cisplatin （DDP） and vincristine （VCR） in vitro respectively and its possible antitumor mechanism. To provide experimental proof for clinical combination application. Methods： Using intermittent administration of high dose VCR, human lung adenocarcinoma sensitive cell line （A549/S） was induced to VCR- resistant human lung adenocarcinoma cell line （A549NCR）. MTT assay was adapted for examing the 50% inhibition （IC50） value of DDP and VCR on A549/S and A549/VCR when quercetin combined with DDP and VCR respectively. Results： IC50 of DDP on A549/S and A549/VCR was 10.18 and 12.35 mg/L, and the IC50 of VCR on the two cell lines was 1.21 and 12.77 rag/L, respectively. The resistance fold of A549/VCR on VCR and DDP was 10.55 and 121, respectively. When quercetin at concentration of 50, 100 and 200 pmol/L in combination with DDP and VCR respectively, the IC50 of DDP and VCR on A549/S and A549/VCR were obvious decreased （P 〈 0.05 - P 〈 0.01）. Conclusion： The experiment results suggested that quercetin could increase the chemosensitivity and partly revise the resistance of A549NCR.
文摘Introduction: The aim of this study is to test the factorial structure and the internal consistency of the 12-items General Health Questionnaire adapted to work-related psychological distress (GHQW). Methods: A validated French version of the GHQ-12 was used and transformed to remind of the occupational context. A sample of 1014 workers completed the GHQW. Internal consis- tency was assessed by Cronbach’s α coefficient. The factorial structure was extracted with an exploratory factorial analysis (EFA). Results: The EFA run on the data yield to a one- factor structure explaining 60.5% of the total variance of the scale. The Cronbach’s alpha showed a very good internal consistency of the scale (α = 0.94). Conclusion: The findings support that the GHQW is a reliable and valid instrument for measuring work-related psychological distress in workers. This work-related version could find some applications in epide-miological research at work, in the study of psychosocial risk factors and in the occupational health physi- cian’s daily activity.
文摘En francais moderne,on constate une extension de l'emploi de “le fait que”.Cela tient au fait que cet emploi facilite beaucoup la construction de certaines phrases.L'emploi de “le fait que” est divers,
文摘Objective To study the effects of different concentrations of Quercetin on nitric oxide (NO) production and membrane fluidity of the injured human umbilical vein vascular endothelial cell line(ECV 304) with hypoxia and the lack of glucose. Methods The experiments were performed in the culture of ECV 304 injured with hypoxia and the lack of glucose in vitro. The releases of intracellular lactate dehydrogenase(LDH) of ECV 304 was measured with automatic biochemistry analysis. NO level of ECV 304 was monitored with colorimetry. The membrane fluidity of ECV 304 was measured with the fluorescence polarization method. Results After ECV 304 was cultured in hypoxia and the the lack of glucose for 24 hours, the release of LDH and the membrane fluidity were increased significantly; NO level was decreased. Preincubation of ECV 304 with 20, 80,160 μ mol·L -1 of Quercetin for 24 hours reduced LDH activity, membrane fluidity and increased the level of NO in hypoxia and the lack of glucose induced ECV 304. Conclusion These results demonstrate that Quercetin can produce the protective effect on hypoxia and the lack of glucose induced injury of ECV 304 by increasing release of NO and changing membrane fluidity.
